| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Jae Han Lee, So Hee Han, You Min Kim, Sung Hyun Kim, Eun Seon Yoo, Joong Seok Woo, Gi Hwan Jung, Soo Hyun Jung, Bum Seok Kim, Ji Youn Jun. Shikonin inhibits proliferation of melanoma cells by MAPK pathway-mediated induction of apoptosis. Bioscience reports. vol 41. issue 1. 2021-07-21. PMID:33403388. |
we examined the anticancer effects of shikonin by in vitro experiments (mtt (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay, 4',6-diamidino-2-phenylindole (dapi) stain, annexin v/ propidium iodide (pi) stain, and protein analysis of apoptosis and mitogen-activated protein kinase (mapk) pathways). |
2021-07-21 |
2023-08-13 |
mouse |
| Xiao Chang, Yu-Ying Lin, Li-Na Bai, Wei Zh. miR-302a-3p suppresses melanoma cell progression via targeting METTL3. Journal of chemotherapy (Florence, Italy). 2021-07-21. PMID:34286671. |
the cell proliferation, cell cycle progression, apoptosis, colony formation, migration, and cell invasion ability were determined using mtt assay, propidium iodide (pi) staining, annexin v/pi flow cytometry, plate clone assay, and transwell migration and invasion assays, respectively. |
2021-07-21 |
2023-08-13 |
Not clear |
| Wangping Li, Chunmei Li, Lijie Ma, Faguang Ji. Resveratrol inhibits viability and induces apoptosis in the small‑cell lung cancer H446 cell line via the PI3K/Akt/c‑Myc pathway. Oncology reports. vol 44. issue 5. 2021-07-19. PMID:32901891. |
h446 cell viability and apoptosis were assessed with mtt and flow cytometry, and the expression of cytochrome c and the pi3k/akt/c‑myc pathway and the nuclear translocation of apoptosis inducing factor (aif) were assessed by western blotting. |
2021-07-19 |
2023-08-13 |
human |
| Hongyan Ma, Ying Liu, Zhengtian Li, Lu Yu, Yang Gao, Xiangmei Ye, Baoyi Yang, Hulun Li, Jinghui Sh. Propofol Protects Against Hepatic Ischemia Reperfusion Injury via Inhibiting Bnip3-Mediated Oxidative Stress. Inflammation. vol 44. issue 4. 2021-07-19. PMID:33496895. |
after the h/r liver cells were cultured and treated with pro, the viability, apoptosis, reactive oxygen species (ros) production, and the levels of lactate dehydrogenase (ldh), malondialdehyde (mda), tnf-α, and il-6 were detected by mtt, flow cytometry, colorimetry, and elisa. |
2021-07-19 |
2023-08-13 |
mouse |
| Dong-Ming Lai, Jiang-Jiang Bi, Yong-Hui Chen, Yu-Di Wu, Qing-Wen Huang, Hai-Jie Li, Sheng Zhang, Zheng Fu, Yi-Xin Ton. CCNI2 plays a promoting role in the progression of colorectal cancer. Cancer medicine. vol 10. issue 6. 2021-07-19. PMID:33620152. |
cell proliferation, colony formation, cell cycle, apoptosis, and mechanism investigation of ccni2 knockdown were investigated by mtt assay, colony formation assay, fluorescence-activated cell sorting, and human apoptosis antibody array, respectively. |
2021-07-19 |
2023-08-13 |
mouse |
| Qun Hang, Jie Lu, Lugen Zuo, Mulin Li. Linc00641 promotes the progression of gastric carcinoma by modulating the miR-429/Notch-1 axis. Aging. vol 13. issue 6. 2021-07-19. PMID:33714199. |
mtt assays, flow cytometry, wound healing assays, and transwell invasion assays were utilized to measure cell viability, apoptosis, migration and invasion, respectively. |
2021-07-19 |
2023-08-13 |
human |
| A Brito, T Santos, K Herculano, M Miranda, A K Sá, J L Carvalho, R Albertini, H Castro-Faria-Neto, A P Ligeiro-de-Oliveira, Flávio Aimbir. The MAPKinase Signaling and the Stimulatory Protein-1 (Sp1) Transcription Factor Are Involved in the Phototherapy Effect on Cytokines Secretion from Human Bronchial Epithelial Cells Stimulated with Cigarette Smoke Extract. Inflammation. vol 44. issue 4. 2021-07-19. PMID:33730343. |
human bronchial epithelial cell (beas) line was cultured in a medium with cse and irradiated (660 nm) at 9 j. apoptosis index was standardized with annexin v and the cellular viability was evaluated by mtt. |
2021-07-19 |
2023-08-13 |
human |
| Degui Shu, Yufen Xu, Wenyu Che. Knockdown of lncRNA BLACAT1 reverses the resistance of afatinib to non-small cell lung cancer via modulating STAT3 signalling. Journal of drug targeting. vol 28. issue 3. 2021-07-16. PMID:31359792. |
mtt assay, colony formation assay, apoptosis analysis, qrt-pcr and western blot analysis, immunohistochemistry, and in vivo study were carried out. |
2021-07-16 |
2023-08-13 |
Not clear |
| Yuanguo Xiong, Hao Lu, Hanlin X. Galangin Reverses Hepatic Fibrosis by Inducing HSCs Apoptosis via the PI3K/Akt, Bax/Bcl-2, and Wnt/β-Catenin Pathway in LX-2 Cells. Biological & pharmaceutical bulletin. vol 43. issue 11. 2021-07-16. PMID:32893252. |
cell viability was analyzed using cell counting kit-8 (cck-8) and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (mtt) assay, cell apoptosis was measured using flow cytometry, and the anti-fibrogenic effect of galangin was determined using rt-quantitative (q)pcr, immunofluorescence, and western blotting. |
2021-07-16 |
2023-08-13 |
Not clear |
| Wei-Ping Zheng, Fan-Long Meng, Lian-Yun Wan. miR-544a Stimulates endometrial carcinoma growth via targeted inhibition of reversion-inducing cysteine-rich protein with Kazal motifs. Molecular and cellular probes. vol 53. 2021-07-14. PMID:32525042. |
functionally, the mir-544a inhibitor restricted the proliferation [detected by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-h-tetrazolium bromide (mtt) assay], invasion, and migration (detected by transwell assay) of human endometrial adenocarcinoma cells (hec-1b and ishikawa) and facilitated cell apoptosis (detected by flow cytometry assay). |
2021-07-14 |
2023-08-13 |
mouse |
| Yameng Fan, Jiaqiao Li, Yuxuan Yang, Xiaodan Zhao, Yamei Liu, Yude Jiang, Long Zhou, Yang Feng, Yan Yu, Yilong Chen. Resveratrol modulates the apoptosis and autophagic death of human lung adenocarcinoma A549 cells via a p53‑dependent pathway: Integrated bioinformatics analysis and experimental validation. International journal of oncology. vol 57. issue 4. 2021-07-14. PMID:32945383. |
cell viability was determined by mtt assay, and flow cytometry and tunel assay were used to evaluate cell apoptosis. |
2021-07-14 |
2023-08-13 |
human |
| Chi Pan, Huijie Duan, Yinan Wu, Chunpeng Zhu, Chenghao Yi, Yin Duan, Demin Lu, Cheng Guo, Deqi Wu, Yanyan Wang, Xianhua Fu, Jing Xu, Yiding Chen, Meng Luo, Wei Tian, Tao Pan, Wenhong Xu, Suzhan Zhang, Jianjin Huan. Inhibition of DNA‑PK by gefitinib causes synergism between gefitinib and cisplatin in NSCLC. International journal of oncology. vol 57. issue 4. 2021-07-14. PMID:32945394. |
mtt assay, apoptosis analysis, tumorsphere formation and an orthotropic xenograft mouse model were used to examine the combination effects of gefitinib and cisplatin on nsclc. |
2021-07-14 |
2023-08-13 |
mouse |
| Xiao Shao, Zhaozheng Liu, Shanshan Liu, Na Lin, Yue Den. Astragaloside IV alleviates atherosclerosis through targeting circ_0000231/miR-135a-5p/CLIC4 axis in AS cell model in vitro. Molecular and cellular biochemistry. vol 476. issue 4. 2021-07-14. PMID:33439448. |
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (mtt) assay and flow cytometry were used to analyze cell viability and apoptosis. |
2021-07-14 |
2023-08-13 |
Not clear |
| Ming Chen, Meng Zhao, Yan Hou, Bin Zh. Expression of lncRNA CCAT2 in children with neuroblastoma and its effect on cancer cell growth. Molecular and cellular biochemistry. vol 476. issue 4. 2021-07-14. PMID:33475889. |
then a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (mtt) assay was carried out to analyze the cell proliferation, migration, and invasion ability, a flow cytometry to detect cell apoptosis, and a western blotting (wb) assay to quantify p53 and bcl-2 proteins. |
2021-07-14 |
2023-08-13 |
human |
| Fatemeh Hajipour, Masoud Mahdavinia, Masood Fereidoonnezha. Half-lantern Cyclometalated Platinum(II) Complexes as Anticancer Agents: Molecular docking, Apoptosis, Cell Cycle Analysis and Cytotoxic Activity Evaluations. Anti-cancer agents in medicinal chemistry. 2021-07-14. PMID:34259151. |
in this study, the anticancer activity of half-lantern cyclometalated pt(ii)‒pt(ii) complexes were was evaluated using mtt, apoptosis, cell cycle analysis, and dna binding studies. |
2021-07-14 |
2023-08-13 |
Not clear |
| Jiejie Yang, Bin Wang, Qian Xu, Yuling Yang, Lin Hou, Kan Yin, Qingming Guo, Yanan Hua, Li Zhang, Yixuan Li, Jinyu Zhang, Ning L. TMEM166 inhibits cell proliferation, migration and invasion in hepatocellular carcinoma via upregulating TP53. Molecular and cellular biochemistry. vol 476. issue 2. 2021-07-13. PMID:33200377. |
to investigate its biological function and underlying mechanism in hcc, tmem166 was overexpressed in hcc cell lines and assessed its effects on cell proliferation, migration, invasion, apoptosis and cell cycle by mtt assay, wound healing assay, transwell assay, annexin v-fitc/pi assay, jc-1 staining and flow cytometry assay, respectively. |
2021-07-13 |
2023-08-13 |
Not clear |
| Jia Xu, Xiangdong Ma, Hong Yang, Junru Zhang, Guoqing Cai, Nianling Ya. MiR-509-3p Induces Apoptosis and Affects the Chemosensitivity of Cervical Cancer Cells by Targeting the RAC1/PAK1/LIMK1/Cofilin Pathway. Chemical & pharmaceutical bulletin. vol 69. issue 4. 2021-07-13. PMID:33790078. |
additionally, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (mtt) assay and flow cytometry were performed to verify the viability and apoptosis of hcc94 and c-33a cells after upregulating mir-509-3p. |
2021-07-13 |
2023-08-13 |
Not clear |
| Bin Zhang, Jie Chen, Ming Cui, Yong Jian. LncRNA ZFAS1/miR-1271-5p/HK2 Promotes Glioma Development Through Regulating Proliferation, Migration, Invasion and Apoptosis. Neurochemical research. vol 45. issue 12. 2021-07-12. PMID:32964288. |
the cell proliferation, apoptosis, and mobility ability were assessed with 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl-2h-tetrazol-3-ium bromide (mtt), flow cytometry, and transwell assays. |
2021-07-12 |
2023-08-13 |
Not clear |
| Zhanqiang Zheng, Guanglin Zhang, Xiaodong Liang, Tianxiao L. LncRNA OIP5-AS1 facilitates ox-LDL-induced endothelial cell injury through the miR-98-5p/HMGB1 axis. Molecular and cellular biochemistry. vol 476. issue 1. 2021-07-12. PMID:32990894. |
3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-h-tetrazolium bromide (mtt) assay and flow cytometry were used to detect the cell proliferation and apoptosis. |
2021-07-12 |
2023-08-13 |
human |
| Yunjuan Zhang, Zengyan Cai, Jin Liang, Erqing Chai, Anqing Lu, Yinwu Shan. CircCPA4 Promotes the Malignant Phenotypes in Glioma via miR-760/MEF2D Axis. Neurochemical research. vol 45. issue 12. 2021-07-12. PMID:33068224. |
cell proliferation, apoptosis, migration, and invasion were analyzed using mtt (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide), colony formation, flow cytometry, and transwell assays, respectively. |
2021-07-12 |
2023-08-13 |
Not clear |