| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Rong Ding, Wenyue Kang, Duozhi Wu, Lin Wan. Protective effect of propofol via the regulation of ovarian granulosa cell proliferation and apoptosis. Experimental and therapeutic medicine. vol 22. issue 3. 2021-08-06. PMID:34345270. |
human ovarian granulosa cell-like kgn cells, which were used as a representative of granulosa cells in the present study, were treated with different concentrations (0, 1, 5 and 10 µg/ml) of propofol for 48 h and cell proliferation and apoptosis were assessed using mtt and flow cytometry assays, respectively. |
2021-08-06 |
2023-08-13 |
human |
| Rong Ding, Wenyue Kang, Duozhi Wu, Lin Wan. Protective effect of propofol via the regulation of ovarian granulosa cell proliferation and apoptosis. Experimental and therapeutic medicine. vol 22. issue 3. 2021-08-06. PMID:34345270. |
kgn cells were transfected with mir-451a inhibitor or inhibitor control sequences for 6 h and treated with 10 µg/ml propofol for an additional 48 h. the results from the mtt, rt-qpcr and western blot assays indicated that 10 µg/ml propofol inhibited cell viability, induced apoptosis, enhanced cleaved caspase 3 expression, reduced pro-caspase 3 levels and inhibited the protein and mrna expression of wnt3a and β-catenin. |
2021-08-06 |
2023-08-13 |
human |
| Rongjun Gao, Hui Ye, Qingjun Gao, Nanpeng Wang, Yan Zhou, Haisong Dua. Inhibition of circular RNA_0000285 prevents cell proliferation and induces apoptosis in thyroid cancer by sponging microRNA-654-3p. Oncology letters. vol 22. issue 3. 2021-08-06. PMID:34345298. |
thyroid cancer cell viability and apoptosis were determined via the mtt assay and flow cytometric analysis, respectively, whereas the expression levels of circrna_0000285 and mir-654-3p were determined via reverse transcription-quantitative pcr analysis. |
2021-08-06 |
2023-08-13 |
Not clear |
| L I Dong, Yumin Zheng, Lianbo Gao, Xiaoguang Lu. lncRNA NEAT1 prompts autophagy and apoptosis in MPTP-induced Parkinson's disease by impairing miR-374c-5p. Acta biochimica et biophysica Sinica. vol 53. issue 7. 2021-08-05. PMID:33984130. |
the cellular viability and apoptosis of sh-sy5y were detected by mtt assay and flow cytometric analysis, respectively, and the number of autophagosomes was subsequently measured by transmission electron microscopy. |
2021-08-05 |
2023-08-13 |
mouse |
| Jing Zhao, Peifeng Li, Hua Zhu, Fengqin Ge, Jie Liu, Jingjun Xia, Pengzhou Han. 7,8-Dihydroxyflavone suppresses proliferation and induces apoptosis of human osteosarcoma cells. Acta biochimica et biophysica Sinica. vol 53. issue 7. 2021-08-05. PMID:34019097. |
human os cell lines (u2os and 143b) were treated with 7,8-dhf; cell viability and cell migration were assessed by methylthiazolyldiphenyl-tetrazolium bromide (mtt) assay and wound-healing assay, respectively; and cell death and apoptosis were evaluated by live/dead staining and terminal deoxynucleotidyl transferase (tdt) dutp nick-end labeling (tunel) assay, respectively. |
2021-08-05 |
2023-08-13 |
human |
| Jilong Hu, Zhinan Zheng, Jia Lei, Yuxin Cao, Qiyun Li, Zhi Zheng, Chuanjun Che. Targeting the EZH2-PPAR Axis Is a Potential Therapeutic Pathway for Pancreatic Cancer. PPAR research. vol 2021. 2021-08-03. PMID:34335707. |
in vitro, pc cell lines panc-1 and aspc-1 were cultured, and mtt and flow cytometry were performed to observe the effects of pan-ppar agonist bezafibrate and ezh2 selective inhibitor gsk126 on cell viability and apoptosis. |
2021-08-03 |
2023-08-13 |
Not clear |
| Ji Li, Qing Xiang, Mei Wang, Hongchang Zhang, Rong Lian. All-Trans Retinoic Acid Enhances Chemosensitivity to 5-FU by Targeting miR-378c/E2F7 Axis in Colorectal Cancer. Journal of oncology. vol 2021. 2021-08-03. PMID:34335757. |
in this study, we utilized mtt (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide), transwell assay, and flow cytometry to confirm that atra was able to enhance the inhibitory effects of 5-fu on hct116 cells effectively by promoting cell apoptosis. |
2021-08-03 |
2023-08-13 |
Not clear |
| Ting-Yu Wu, Qin Leng, Li-Qun Tia. The microRNA-210/Casp8ap2 Axis Alleviates Hypoxia-Induced Myocardial Injury by Regulating Apoptosis and Autophagy. Cytogenetic and genome research. vol 161. issue 3-4. 2021-08-02. PMID:33882492. |
mtt was used to evaluate cell survival, and flow cytometry was used to assess apoptosis and the cell cycle distribution. |
2021-08-02 |
2023-08-13 |
Not clear |
| Ping Xiang, Youwen Sun, Zhiqing Fang, Keqiang Yan, Yidong Fa. Eukaryotic translation initiation factor 3 subunit b is a novel oncogenic factor in prostate cancer. Mammalian genome : official journal of the International Mammalian Genome Society. vol 31. issue 7-8. 2021-07-30. PMID:32556998. |
small interfering rna (sirna) knockdown of eif3b was introduced into prostate cancer cell line pc-3 and lncap, followed by examination of cell viability, proliferation and apoptosis using the mtt, cell counting and terminal deoxynucleotidyl transferase dutp nick end labeling assays, respectively. |
2021-07-30 |
2023-08-13 |
mouse |
| Juan He, Huaping Zhao, Xing Liu, Dongmei Wang, Yong Wang, Yanqiu Ai, Jianjun Yan. Sevoflurane suppresses cell viability and invasion and promotes cell apoptosis in colon cancer by modulating exosome‑mediated circ‑HMGCS1 via the miR‑34a‑5p/SGPP1 axis. Oncology reports. vol 44. issue 6. 2021-07-30. PMID:33125091. |
mtt assay, flow cytometric analysis and transwell assay were conducted to evaluate cell viability, apoptosis and invasion, respectively. |
2021-07-30 |
2023-08-13 |
human |
| Yalda Baghay Esfandyari, Mohammad Amin Doustvandi, Mohammad Amini, Behzad Baradaran, Sheyda Jodeiry Zaer, Nazila Mozammel, Mehdi Mohammadzadeh, Ahad Mokhtarzade. MicroRNA-143 Sensitizes Cervical Cancer Cells to Cisplatin: a Promising Anticancer Combination Therapy. Reproductive sciences (Thousand Oaks, Calif.). vol 28. issue 7. 2021-07-30. PMID:33569751. |
the cell viability and apoptosis induction were evaluated by mtt, annexin v-fitc/pi, and dapi staining tests. |
2021-07-30 |
2023-08-13 |
Not clear |
| Leila Soufi, Alireza Farasat, Hossein Ahmadpour-Yazdi, Leila Zolghadr, Nematollah Gheib. The effects of the esterified Quercetin with omega3 and omega6 fatty acids on viability, nanomechanical properties, and BAX/BCL-2 gene expression in MCF-7 cells. Molecular biology reports. vol 48. issue 6. 2021-07-29. PMID:34254227. |
using the mtt and flow cytometry assays, the cytotoxic potential, apoptosis, and necrosis were investigated. |
2021-07-29 |
2023-08-13 |
Not clear |
| Bin Huang, Gongzhi Wu, Chongxiong Peng, Xuyang Peng, Mingjiang Huang, Jianyang Ding, Huaizhong Zhang, Xuhui W. miR-126 regulates the proliferation, migration, invasion, and apoptosis of non-small lung cancer cells via AKT2/HK2 axis. IUBMB life. 2021-07-28. PMID:34320278. |
win vitro experiments including 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-h-tetrazolium bromide (mtt), wound healing assay, transwell, and flow cytometry assay were used for evaluating the effect of mir-126 on cell proliferation, migration, invasion, and apoptosis. |
2021-07-28 |
2023-08-13 |
Not clear |
| Qiang Wang, Peigang Chu, Xia Yu, Jun Li, Wenzheng Zhang, Mingzhi Gon. ZFAS1 knockdown inhibits fibroblast-like synoviocyte proliferation, migration, invasion and inflammation, and promotes apoptosis via miR-3926/FSTL1 in rheumatoid arthritis. Experimental and therapeutic medicine. vol 22. issue 3. 2021-07-27. PMID:34306188. |
mtt assay, flow cytometric analysis and transwell assay were performed to examine the proliferation, apoptosis, migration and invasion of fibroblast-like synoviocytes (flss), respectively. |
2021-07-27 |
2023-08-13 |
human |
| Jin Zhang, Wei Hua, Xinyuan Zhao, Fan Yang, Ting Guo, Jianhua Zhang, Xuerong Zheng, Wanqi Lian. Paeoniflorin alleviates endothelial dysfunction caused by overexpression of soluble fms-like tyrosine kinase 1 and soluble endoglin in preeclampsia via VEGFA upregulation. Bioscience, biotechnology, and biochemistry. vol 85. issue 4. 2021-07-26. PMID:33590855. |
cell viability, migration, invasion, tube formation, and apoptosis were assessed by (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) tetrazolium mtt assay, scratch assay, transwell assay, tube formation assay, and flow cytometry. |
2021-07-26 |
2023-08-13 |
human |
| Difang Chen, Kunwei Wang, Yan Zheng, Guangyu Wang, Mei Jian. Exosomes-Mediated LncRNA ZEB1-AS1 Facilitates Cell Injuries by miR-590-5p/ETS1 Axis Through the TGF-β/Smad Pathway in Oxidized Low-density Lipoprotein-induced Human Umbilical Vein Endothelial Cells. Journal of cardiovascular pharmacology. vol 77. issue 4. 2021-07-26. PMID:33818551. |
cell proliferation and apoptosis were assessed by mtt assay and flow cytometry analysis, respectively. |
2021-07-26 |
2023-08-13 |
human |
| Zhuangzhi Wu, Xiaoning He, Siqi Che. Oncogenic circDHTKD1 promotes tumor growth and metastasis of oral squamous cell carcinoma in vitro and in vivo via upregulating miR-326-mediated GAB1. Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas. vol 54. issue 10. 2021-07-23. PMID:34287578. |
cellular behaviors were detected by 3-(4, 5-dimethylthiazol-2-y1)-2,5-diphenyl tetrazolium bromide (mtt) for cell viability, colony formation assay for clonal capacity, flow cytometry for cell apoptosis, wound healing assay for migration, and transwell assay for migration/invasion. |
2021-07-23 |
2023-08-13 |
mouse |
| Chen-Jun Zhang, Yu Huang, Ji-De Lu, Jie Lin, Zhi-Ru Ge, Hui Huan. Upregulated microRNA-132 rescues cardiac fibrosis and restores cardiocyte proliferation in dilated cardiomyopathy through the phosphatase and tensin homolog-mediated PI3K/Akt signal transduction pathway. Journal of cellular biochemistry. 2021-07-21. PMID:30216493. |
cell proliferation and apoptosis were tested by the mtt assay and flow cytometry. |
2021-07-21 |
2023-08-13 |
rat |
| Zun-Wen Lin, Wei Zhang, Si-Ding Jiang, Wen-Bo Wei, Xiao-Feng L. Inhibition of microRNA-940 suppresses the migration and invasion of human osteosarcoma cells through the secreted frizzled-related protein 1-mediated Wnt/β-catenin signaling pathway. Journal of cellular biochemistry. 2021-07-21. PMID:30324663. |
mtt (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) assay, scratch test, transwell assay, and flow cytometry were carried out to detect proliferation, migration, invasion, and apoptosis, respectively. |
2021-07-21 |
2023-08-13 |
mouse |
| Dan Wang, Suhong Zhang, Min Zhao, Fengling Che. LncRNA MALAT1 accelerates non-small cell lung cancer progression via regulating miR-185-5p/MDM4 axis. Cancer medicine. vol 9. issue 23. 2021-07-21. PMID:33146951. |
cell proliferation and apoptosis were, respectively, determined by 3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2-h-tetrazolium bromide (mtt) assay, and flow cytometry. |
2021-07-21 |
2023-08-13 |
mouse |