| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Hong-Yan Tong, Mao-Fang Li. [Effect of 5-aza-2'-deoxycytidine on cell of high-risk patients with myelodysplastic syndrome in vitro]. Zhongguo shi yan xue ye xue za zhi. vol 12. issue 4. 2005-01-26. PMID:15363133. |
to investigate the effect of 5-aza-2'-deoxycytidine (5-aza-cdr) on cell of high-risk patients with myelodysplastic syndrome (mds) in vitro, the growth inhibition of mutz-1 cell induced by 5-aza-cdr was detected by mtt method; apoptosis was detected by morphological observation and translocation of phosphatidylserine (ps) was examined by flow cytometry assay; the expressions of p15ink4b, dna methyltransferases (dnmt)(1), dnmt(3a) and dnmt(3b) gene on mrna level were detected by rt-pcr; methylation of p15ink4b gene in mutz-1 cells was detected by pcr using methylation specific primer (msp). |
2005-01-26 |
2023-08-12 |
Not clear |
| Zhi-Gang Peng, Jun Luo, Ling-Hui Xia, Yan Chen, San-Jun Son. [CML cell line K562 cell apoptosis induced by mangiferin]. Zhongguo shi yan xue ye xue za zhi. vol 12. issue 5. 2005-01-26. PMID:15498116. |
the antiproliferation effects of mangiferin on k562 leukemia cells were tested by tetrazolium salt (mtt) method; the apoptosis induced by mangiferin on k562 cell line was explored by means of cell morphology, dna gel electrophoresis and flow cytometry. |
2005-01-26 |
2023-08-12 |
Not clear |
| Jia Fei, Huan Zhan. [Effect and mechanism of VEGF antisense phosphorothioate deoxynucleotides on HL-60 leukemic cells]. Zhongguo shi yan xue ye xue za zhi. vol 12. issue 5. 2005-01-26. PMID:15498126. |
after culture for 72 hours, inhibitive rate of cell growth was detected with mtt methods, viable cells were counted with trypan blue exclusion each 24 hour, cell configuration and apoptosis were observed with geimsa staining and flow cytometry respectively, level of vegf protein was detected with vegf elisa kit. |
2005-01-26 |
2023-08-12 |
Not clear |
| Ya-Wun Yang, Ching-An Wu, W J W Morro. The apoptotic and necrotic effects of tomatine adjuvant. Vaccine. vol 22. issue 17-18. 2005-01-18. PMID:15149791. |
cytotoxicity was monitored using the mtt [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] and lactate dehydrogenase release assays, apoptosis and necrosis were quantified by flow cytometry using annexin v and propidium iodide staining, and morphology was examined by hoechst 33342 staining. |
2005-01-18 |
2023-08-12 |
Not clear |
| Bettina Zödl, Mansour Sargazi, Michaela Zeiner, Norman B Roberts, Ilse Steffan, Wolfgang Marktl, Cem Ekmekciogl. Toxicological effects of iron on intestinal cells. Cell biochemistry and function. vol 22. issue 3. 2004-11-24. PMID:15124177. |
cellular damage was assessed using cell proliferation (serial cell counting), tetrazolium dye (mtt) uptake, lactate dehydrogenase (ldh) release and apoptosis studies based on caspase-3 activities. |
2004-11-24 |
2023-08-12 |
human |
| Wei-Kai Chen, Yan Chen, Jun-Xia Gu, Guo-Hui Cu. [Effect of trichostatin A on histone acetylation level and apoptosis in HL-60 cells]. Zhongguo shi yan xue ye xue za zhi. vol 12. issue 3. 2004-10-28. PMID:15228659. |
in order to explore the underlying mechanism of high effects and low toxicity of trichostatin a (tsa), the effect of tsa on growth inhibition, histone acetylation level and apoptosis in hl-60 cells and normal human peripheral blood mononuclear cells (npbmnc) were examined using mtt method, immunocytochemistry technology, and annexin-v-fitc/pi double staining flow cytometry. |
2004-10-28 |
2023-08-12 |
human |
| Ze-Lin Liu, Jian-Min Luo, Zuo-Ren Dong, Fu-Xu Wang, Xue-Jun Zhang, Jing-Ci Yang, Xing-Yan Du, Li Ya. [In vitro effects of mevastatin on the proliferation and apoptosis in human multiple myeloma cell line U266]. Zhongguo shi yan xue ye xue za zhi. vol 12. issue 3. 2004-10-28. PMID:15228663. |
in order to investigate the anti-tumor activity of 3-hydroxy-3-methylglutaryl coenzyme a (hmg-coa) reductase inhibitors and the mechanism underlying the cell proliferation and apoptosis modulated in myeloma cells, the effects of mevastatin, an hmg-coa reductase inhibitor, on cell growth, cell cycle progression and apoptosis in u266 human multiple myeloma (mm) cell line in vitro were explored by mtt colorimetric assay, morphologic observation, flow cytometry, dna gel electrophoresis, and rt-pcr. |
2004-10-28 |
2023-08-12 |
human |
| Min Zhang, Fang Liu, Wei He, Yong You, Ping Zou, Ling-Bo Li. Inhibition of Fas-mediated apoptosis in Yac-1 cell via Anti-Fas ribozyme. Acta biochimica et biophysica Sinica. vol 36. issue 3. 2004-10-19. PMID:15202504. |
after treated with anti-fas antibody (jo2), yac-1 cell viability was measured with mtt assay, caspase-3 proteolytic activity was detected, and cell apoptosis was measured according to annexin v apoptosis detecting kit. |
2004-10-19 |
2023-08-12 |
mouse |
| Sai Latha Shankar, Melissa Krupski, Bhupesh Parashar, Chidi Okwuaka, Kathy O'Guin, Sridhar Mani, Bridget Shafit-Zagard. UCN-01 alters phosphorylation of Akt and GSK3beta and induces apoptosis in six independent human neuroblastoma cell lines. Journal of neurochemistry. vol 90. issue 3. 2004-09-10. PMID:15255949. |
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (mtt), terminal deoxynucleotidyl transferase-mediated dutp nick-end-labeling (tunel) assays, detection of active caspase-3 and cleaved poly adp-ribose polymerase (parp) confirmed that ucn-01 induced apoptosis. |
2004-09-10 |
2023-08-12 |
human |
| Christina Löfgren, Linn Hjortsberg, Malin Blennow, Kourosh Lotfi, Christer Paul, Staffan Eriksson, Freidoun Albertion. Mechanisms of cross-resistance between nucleoside analogues and vincristine or daunorubicin in leukemic cells. Biochemical and biophysical research communications. vol 320. issue 3. 2004-08-26. PMID:15240122. |
all resistant strains exhibited cross-resistance to na (cladribine and cytosine arabinoside)-induced apoptosis, assessed by caspase-3-like activation and were less sensitive to na cytotoxicity in mtt assay. |
2004-08-26 |
2023-08-12 |
Not clear |
| Nai-Kuei Huang, Yun-Lian Lin, Jing-Jy Cheng, Wen-Lin La. Gastrodia elata prevents rat pheochromocytoma cells from serum-deprived apoptosis: the role of the MAPK family. Life sciences. vol 75. issue 13. 2004-08-25. PMID:15261768. |
in this study, differential methanol (meoh) extracts of g. elata were found to prevent serum-deprived rat pheochromocytoma (pc12) cell apoptosis by the mtt assay and hoechst staining. |
2004-08-25 |
2023-08-12 |
rat |
| Xiu-jin Ye, Mao-fang Li. Homoharringtonine induces apoptosis of endothelium and down-regulates VEGF expression of K562 cells. Journal of Zhejiang University. Science. vol 5. issue 2. 2004-08-23. PMID:14674038. |
cellular proliferation was determined by mtt assay and apoptosis was analyzed by flow cytometry. |
2004-08-23 |
2023-08-12 |
human |
| Saiko Asahara, Asomi Sato, Ahmed Ali Aljonaid, Takeshi Maru. Thyroid hormone synergizes with follicle stimulating hormone to inhibit apoptosis in porcine granulosa cells selectively from small follicles. The Kobe journal of medical sciences. vol 49. issue 5-6. 2004-08-17. PMID:15141146. |
relative viability, proliferative activity, and apoptosis of cultured granulosa cells were evaluated with 3-(4.5-dimethylahiazol-2-yl)-2,5-diphenyltetrazolium bromide [mtt] assay, ki67 expression and activated caspase-3 protein expression, respectively. |
2004-08-17 |
2023-08-12 |
Not clear |
| Hiromi Sekido, Takeshi Suzuki, Takahito Jomori, Masayoshi Takeuchi, Chihiro Yabe-Nishimura, Soroku Yagihash. Reduced cell replication and induction of apoptosis by advanced glycation end products in rat Schwann cells. Biochemical and biophysical research communications. vol 320. issue 1. 2004-08-13. PMID:15207727. |
age-2 and -3, but not age-1, induced apoptosis, and significantly decreased the viability measured by mtt assay. |
2004-08-13 |
2023-08-12 |
rat |
| Wei Xiang, Xianli Zhu, Hongyang Zha. The effects of wild-type p53 gene transfection on the growth and chemotherapeutic sensitivity of human glioma cells. Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban. vol 22. issue 1. 2004-08-09. PMID:12658781. |
p53 gene expression in transfected cells was detected by rt-pcr, the cell growth inhibition and apoptosis in either the absence or the presence of cisplatin was assessed by mtt and flow cytometry. |
2004-08-09 |
2023-08-12 |
human |
| Wei Xue, Shuhua Du, Yong Li, Yejing Yang, Jinghua Su. Effects of nitric oxide on proliferation and apoptosis of cultured bovine trabecular meshwork cells. Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban. vol 22. issue 1. 2004-08-09. PMID:12658790. |
no2- in supernate, the proliferation and apoptosis of tm cells and mrna expression of bcl-2 and bax were measured by griess reagent, terminal deoxynucleotidyl transferase mediated dutp nick end labeling (tunel), mtt assay and in situ hybridization, respectively. |
2004-08-09 |
2023-08-12 |
cattle |
| Jin-Woo Park, Mariwil G Wong, Margaret Lobo, William C Hyun, Quan-Yang Duh, Orlo H Clar. Modulation of tumor necrosis factor-related apoptosis-inducing ligand-induced apoptosis by chemotherapy in thyroid cancer cell lines. Thyroid : official journal of the American Thyroid Association. vol 13. issue 12. 2004-08-04. PMID:14751030. |
we used flow cytometry to measure apoptosis, dimethyl-thiazol-diphenyltetrazolium bromide (mtt) assay to measure antiproliferation effects and western blot to determine the expression of bcl family proteins. |
2004-08-04 |
2023-08-12 |
human |
| J H Yang, F Feng, H Qian, H Chen. Chemosensitization of breast carcinoma cells with the use of bcl-2 antisense oligodeoxynucleotide. Breast (Edinburgh, Scotland). vol 13. issue 3. 2004-07-30. PMID:15177426. |
changes in expression of the bcl-2 gene were examined by western blot; cell growths were detected by mtt assay, and apoptosis rates were detected by flow cytometry (fcm). |
2004-07-30 |
2023-08-12 |
Not clear |
| Min Zhang, Ping Zou, Ming Bai, Yang Jin, Xiaonan Ta. Peroxisome proliferator-activated receptor-gamma activated by ligands can inhibit human lung cancer cell growth through induction of apoptosis. Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban. vol 23. issue 2. 2004-07-22. PMID:12973930. |
to study the expression of peroxisome proliferator-activated receptor-gamma (ppar-gamma) in lung cancer cells, and to testify if the ppar-gamma agonists can inhibit human lung cancer cell growth through induction of apoptosis, ppar-gamma was detected in two lung cancer cell lines by rt-pcr and immunohistochemistry, the inhibition of human lung cancer cell growth was investigated by mtt and cell counts, and the apoptosis was assessed by tunel. |
2004-07-22 |
2023-08-12 |
human |
| Zhongzhi Tang, Ying Tang, Libo F. Growth inhibition and apoptosis induction in human hepatoma cells by tanshinone II A. Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban. vol 23. issue 2. 2004-07-22. PMID:12973939. |
in order to study the effect of tanshinone ii a on growth and apoptosis in human hepatoma cell line bel-7402 in vitro, the human hepatoma cell line bel-7402 was treated with tanshinone ii a at various concentrations for 72 h. growth suppression was evaluated by mtt assay; apoptosis-related alterations in morphology and biochemistry were ascertained under cytochemical staining (hoechst 33258), transmission electron microscopy (tem), and dna agarose gel electrophoresis. |
2004-07-22 |
2023-08-12 |
human |