| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Ze-Lin Liu, Zuo-Ren Dong, Fu-Xu Wang, Xue-Jun Zhang, Jing-Ci Yang, Wei-Dong Ma, Xing-Yan Du, Li Ya. [Effects of sodium orthovanadate on proliferation and apoptosis in raji cells and its mechanism]. Zhongguo shi yan xue ye xue za zhi. vol 10. issue 4. 2003-07-23. PMID:12513765. |
results showed that mtt assay and cfu-raji culture demonstrated that sodium or thovanadate inhibited the growth of raji cells in a concentration-dependent fashion; morphologic observations showed that raji cells exhibited cytoplasm shrinkage, cytoplasm membrane blebbing, nuclear fragmentation and chromatin condensation forming crescents along nuclear membrane characteristic of apoptosis in the presence of na(3)vo(4); dna gel electrophoresis revealed typical dna ladder reminiscent of dna cleavage at internucleosomal sites in na(3)vo(4) treated cells; fcm and rt-pcr indicated that na(3)vo(4) intervention increased the fraction of annexin v(+) pi(-) cells, reduced the value of mitochondrial transmembrane potential, induced g(2)/m arrest and down-regulated the expression of bcl-2 and cyclin b1 at both mrna and protein level in a concentration-dependent manner. |
2003-07-23 |
2023-08-12 |
Not clear |
| S F Picton, J T McCluskey, P R Flatt, N H McClenagha. Effects of cytotoxic agents on functional integrity and antioxidant enzymes in clonal beta-cells. Diabetes & metabolism. vol 28. issue 6 Pt 2. 2003-07-07. PMID:12688636. |
cell viability was determined using the mtt colorimetric assay and the tunel assay was used to assess apoptosis and acridine orange assay was used to determine levels of apoptosis versus necrosis. |
2003-07-07 |
2023-08-12 |
cat |
| Ahmed M Shoieb, Mona Elgayyar, Paul S Dudrick, John L Bell, Patricia K Titho. In vitro inhibition of growth and induction of apoptosis in cancer cell lines by thymoquinone. International journal of oncology. vol 22. issue 1. 2003-06-16. PMID:12469192. |
tq-induced cytotoxicity was determined using a proliferation assay (mtt assay) and apoptosis assays. |
2003-06-16 |
2023-08-12 |
human |
| Yuhong He, Jing Chen, Jianguo Ren, Guochun Wang, Guoping Ca. Type I collagen inhibits hydroxyl radical-induced apoptosis. Journal of biochemistry. vol 132. issue 3. 2003-05-16. PMID:12204105. |
further study showed that type i collagen also inhibited cell apoptosis induced by.oh, as evidenced by morphological criteria (dapi and annexin v staining) and quantitive assays for apoptotic cells (mtt and flow-cytometric assay for subg1 cells). |
2003-05-16 |
2023-08-12 |
Not clear |
| Jennifer Y Kong, Simon W Rabki. Mitochondrial effects with ceramide-induced cardiac apoptosis are different from those of palmitate. Archives of biochemistry and biophysics. vol 412. issue 2. 2003-05-13. PMID:12667483. |
in embryonic chick cardiomyocytes, ceramide, 100 microm for 24h, induced a 42.9+/-5.8% increase in cell death assessed by the mtt assay, and a significant (p<0.01) 3.9+/-0.6-fold increase in apoptosis assessed by propidium iodide staining of permeabilized cells. |
2003-05-13 |
2023-08-12 |
chicken |
| Jin-Won Hyun, Gi-Jeong Cheon, Hyun-Sook Kim, Yun-Sil Lee, Eun-Young Choi, Byung-Hak Yoon, Jeong-Soon Kim, Myung-Hee Chun. Radiation sensitivity depends on OGG1 activity status in human leukemia cell lines. Free radical biology & medicine. vol 32. issue 3. 2003-05-09. PMID:11827746. |
these were: a dramatic arrest at the g2/m phase indicated by a marked increase in both the number of g2/m cells and the expression of cyclin b1, cdc2, and mitotic phosphoprotein monoclonal-2 (mpm-2)-reactive proteins; a severe apoptosis shown by a marked increase in the number of cells with hypo-diploid dna and dna fragmentation; and as a result, a severe inhibition of cell growth and proliferation measured by the mtt test and [(3)h]-thymidine uptake assay. |
2003-05-09 |
2023-08-12 |
human |
| Oskar S Frankfurt, Awtar Krisha. Apoptosis enzyme-linked immunosorbent assay distinguishes anticancer drugs from toxic chemicals and predicts drug synergism. Chemico-biological interactions. vol 145. issue 1. 2003-05-09. PMID:12606157. |
short-term metabolic tetrazolium-based 3-(4,5-dimethylthiazol-yl)-2,5-diphenyformazan bromide (mtt) assay was significantly less sensitive than apoptosis elisa and the cell survival assay. |
2003-05-09 |
2023-08-12 |
Not clear |
| Jeong-Jin Je, Hyun-Taeg Shin, Seok-Hee Chung, Jong-Soo Lee, Sung-Soo Kim, Hyun-Dae Shin, Mi-Hyeon Jang, Youn-Jung Kim, Joo-Ho Chung, Ee-Hwa Kim, Chang-Ju Ki. Protective effects of Wuyaoshunqisan against H2O2-induced apoptosis on hippocampal cell line HiB5. The American journal of Chinese medicine. vol 30. issue 4. 2003-05-07. PMID:12568283. |
to investigate the protective effects of wuyaoshunqisan against h2o2-induced apoptosis in the central nervous system, the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (mtt) assay, the terminal deoxynucleotidyl transferase (tdt)-mediated dutp nick end-labeling (tunel) method, flow cytometric analysis, and the dna fragmentation assay were performed on cells of the hippocampal cell line hib5. |
2003-05-07 |
2023-08-12 |
Not clear |
| Xiang-Dong Gao, Wen-Cai Ye, Albert C H Yu, Yun Zhang, Ren-Xiang Tan, Ming Li, W L Wendy Hsia. Pulsatilloside A and anemoside A3 protect PC12 cells from apoptosis induced by sodium cyanide and glucose deprivation. Planta medica. vol 69. issue 2. 2003-05-07. PMID:12624827. |
additions of pulsatilloside a and anemoside a 3, at dosages ranging from 0.1, 1 and 10 microg/ml, protected pc12 cells from apoptosis determined by mtt, ldh release analysis, and flow cytometry measurement. |
2003-05-07 |
2023-08-12 |
Not clear |
| C G Yuan, L He, X L Xue, Y T Shi, X H B. [Effect of alpha-lipoic acid on the apoptosis of PC12 cells induced by 6-hydroxydopamine]. Shi yan sheng wu xue bao. vol 34. issue 1. 2003-05-06. PMID:12549012. |
it was confirmed by the different percentage of cell survival rate and apoptosis concluded from fcm and mtt. |
2003-05-06 |
2023-08-12 |
rat |
| C G Yuan, L He, X L Xue, Y T Shi, X H B. [Effect of alpha-lipoic acid on the apoptosis of PC12 cells induced by 6-hydroxydopamine]. Shi yan sheng wu xue bao. vol 34. issue 1. 2003-05-06. PMID:12549012. |
the result indicateed that alpha-lipoic acid can partly prevent apoptosis induced by 6-ohda but fail to prevent necrosis since it can decrease the apoptotic cell from 20.09% to 3.09%, just as increased cell survival rate from 56.8% to 72.6% but can not reach the normal level showed by mtt assay. |
2003-05-06 |
2023-08-12 |
rat |
| Mi-Hyeon Jang, Min-Chul Shin, In-Sug Kang, Hyung-Hwan Baik, Yong-Ho Cho, Jong-Phill Chu, Ee-Hwa Kim, Chang-Ju Ki. Caffeine induces apoptosis in human neuroblastoma cell line SK-N-MC. Journal of Korean medical science. vol 17. issue 5. 2003-05-01. PMID:12378022. |
to investigate whether caffeine induces apoptosis in the central nervous system, 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (mtt) assay, 4,6-diamidino-2-phenylindole (dapi) staining, terminal deoxynucleotidyl transferase (tdt)-mediated dutp nick end labeling (tunel) assay, flow cytometric analysis, dna fragmentation assay, and caspase-3 enzyme assay were performed on sk-n-mc human neuroblastoma cells. |
2003-05-01 |
2023-08-12 |
human |
| Akiko Tabuchi, Kouhei Funaji, Jun Nakatsubo, Mamoru Fukuchi, Tomofusa Tsuchiya, Masaaki Tsud. Inactivation of aconitase during the apoptosis of mouse cerebellar granule neurons induced by a deprivation of membrane depolarization. Journal of neuroscience research. vol 71. issue 4. 2003-04-01. PMID:12548706. |
suppression of the apoptosis of cgcs mediated by the ca(2+) influx or neurotrophic factors such as brain-derived neurotrophic factor (bdnf) and adenylate cyclase activating polypeptide-38 (pacap-38) attenuated the aconitase inactivation as well as the lactate dehydrogenase (ldh)-release and the decrease in mtt reduction. |
2003-04-01 |
2023-08-12 |
mouse |
| Mi-Hyeon Jang, Min-Chul Shin, Hye-Sook Shin, Khae-Hawn Kim, Heung-Jae Park, Ee-Hwa Kim, Chang-Ju Ki. Alcohol induces apoptosis in TM3 mouse Leydig cells via bax-dependent caspase-3 activation. European journal of pharmacology. vol 449. issue 1-2. 2003-03-20. PMID:12163104. |
to investigate whether ethanol induces apoptosis in leydig cells, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (mtt) assay, terminal deoxynuclotidyl transferase-mediated dutp nick end-labeling (tunel) assay, 4,6-diamidino-2-phenylindole (dapi) staining, dna fragmentation assay, caspase-3 enzyme assay, reverse transcription-polymerase chain reaction (rt-pcr) and western blot analysis were performed on tm3 mouse leydig cells. |
2003-03-20 |
2023-08-12 |
mouse |
| A L McKeague, D J Wilson, John Nelso. Staurosporine-induced apoptosis and hydrogen peroxide-induced necrosis in two human breast cell lines. British journal of cancer. vol 88. issue 1. 2003-03-14. PMID:12556971. |
staurosporine (ssp)-induced apoptosis and h(2)o(2)-induced necrosis were evaluated by two cytotoxicity assays, neutral red (nr) and methyl-thiazolyl tertrazolium (mtt), in comparison with a differential dye uptake assay, using hoechst33342/propidium iodide (hoechst/pi). |
2003-03-14 |
2023-08-12 |
human |
| A L McKeague, D J Wilson, John Nelso. Staurosporine-induced apoptosis and hydrogen peroxide-induced necrosis in two human breast cell lines. British journal of cancer. vol 88. issue 1. 2003-03-14. PMID:12556971. |
results also showed that neither mtt or nr can distinguish between the modes of cell death, nor detect the early onset of apoptosis revealed by hoechst/pi. |
2003-03-14 |
2023-08-12 |
human |
| Lu Zuo, Jian Li, Ti Shen, Zhi-Nan Zhan. [The comparison between the mechanisms of sodium selenite induced apoptosis and arsenic trioxide induced apoptosis in human acute promyelocytic leukemia cell line NB4 cells]. Zhongguo shi yan xue ye xue za zhi. vol 10. issue 3. 2003-03-10. PMID:12513784. |
in order to explore the differences between the mechanisms of selenite-induced apoptosis and arsenic induced apoptosis in nb4 cells, growth inhibition was determined by mtt test, apoptosis determined by dna electrophoresis and analysis of intracellular dna contents, reactive oxygen species and reduced glutathione in the cell were measured by lucigenin dependent chemoluminescent (cl) test and spectrophotometry, and mitochondrial transmembrane potential was measured by flow cytometry. |
2003-03-10 |
2023-08-12 |
human |
| Hui Yang, Shen-Wu Wang, Hui-Jun Yi. [p21(WAF1) transfection decreases sensitivity of K562 cells to VP-16]. Zhongguo shi yan xue ye xue za zhi. vol 10. issue 1. 2003-02-03. PMID:12513833. |
the k562-p21(waf1) cells were exposed to vp-16 at different concentrations and different times, then, the sensitivity to vp-16 was examined by cell viability and mtt assay, the apoptosis induced by vp-16 was examined by dna fragments electrophoresis and flow cytometric annexin v-pi dual labeling technique. |
2003-02-03 |
2023-08-12 |
Not clear |
| Gabriella Lupo, Giovanna Assero, Carmelina D Anfuso, Ambra Nicotra, Maddalena Palumbo, Giuseppe Cannavò, Marcella Renis, Nicola Ragusa, Mario Alberghin. Cytosolic phospholipase A2 mediates arachidonoyl phospholipid hydrolysis in immortalized rat brain endothelial cells stimulated by oxidized LDL. Biochimica et biophysica acta. vol 1585. issue 1. 2003-01-08. PMID:12457711. |
cell lysates and subcellular fractions were assayed for cpla(2) while the cytotoxic effect and apoptosis were monitored by morphological changes, trypan blue dye exclusion, mtt reduction test, caspase-3 activity, comet and laser confocal fluorescence microscopy (lcfm) analyses. |
2003-01-08 |
2023-08-12 |
rat |
| Rui F M Silva, Cecília M P Rodrigues, Dora Brite. Rat cultured neuronal and glial cells respond differently to toxicity of unconjugated bilirubin. Pediatric research. vol 51. issue 4. 2002-11-26. PMID:11919342. |
ucb toxicity was evaluated by lactate dehydrogenase release, induction of apoptosis, cytoskeleton degeneration, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (mtt) reduction, and glutamate uptake. |
2002-11-26 |
2023-08-12 |
rat |