| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Hiromi Sekido, Takeshi Suzuki, Takahito Jomori, Masayoshi Takeuchi, Chihiro Yabe-Nishimura, Soroku Yagihash. Reduced cell replication and induction of apoptosis by advanced glycation end products in rat Schwann cells. Biochemical and biophysical research communications. vol 320. issue 1. 2004-08-13. PMID:15207727. |
age-2 and -3, but not age-1, induced apoptosis, and significantly decreased the viability measured by mtt assay. |
2004-08-13 |
2023-08-12 |
rat |
| Wei Xiang, Xianli Zhu, Hongyang Zha. The effects of wild-type p53 gene transfection on the growth and chemotherapeutic sensitivity of human glioma cells. Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban. vol 22. issue 1. 2004-08-09. PMID:12658781. |
p53 gene expression in transfected cells was detected by rt-pcr, the cell growth inhibition and apoptosis in either the absence or the presence of cisplatin was assessed by mtt and flow cytometry. |
2004-08-09 |
2023-08-12 |
human |
| Wei Xue, Shuhua Du, Yong Li, Yejing Yang, Jinghua Su. Effects of nitric oxide on proliferation and apoptosis of cultured bovine trabecular meshwork cells. Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban. vol 22. issue 1. 2004-08-09. PMID:12658790. |
no2- in supernate, the proliferation and apoptosis of tm cells and mrna expression of bcl-2 and bax were measured by griess reagent, terminal deoxynucleotidyl transferase mediated dutp nick end labeling (tunel), mtt assay and in situ hybridization, respectively. |
2004-08-09 |
2023-08-12 |
cattle |
| Jin-Woo Park, Mariwil G Wong, Margaret Lobo, William C Hyun, Quan-Yang Duh, Orlo H Clar. Modulation of tumor necrosis factor-related apoptosis-inducing ligand-induced apoptosis by chemotherapy in thyroid cancer cell lines. Thyroid : official journal of the American Thyroid Association. vol 13. issue 12. 2004-08-04. PMID:14751030. |
we used flow cytometry to measure apoptosis, dimethyl-thiazol-diphenyltetrazolium bromide (mtt) assay to measure antiproliferation effects and western blot to determine the expression of bcl family proteins. |
2004-08-04 |
2023-08-12 |
human |
| J H Yang, F Feng, H Qian, H Chen. Chemosensitization of breast carcinoma cells with the use of bcl-2 antisense oligodeoxynucleotide. Breast (Edinburgh, Scotland). vol 13. issue 3. 2004-07-30. PMID:15177426. |
changes in expression of the bcl-2 gene were examined by western blot; cell growths were detected by mtt assay, and apoptosis rates were detected by flow cytometry (fcm). |
2004-07-30 |
2023-08-12 |
Not clear |
| Min Zhang, Ping Zou, Ming Bai, Yang Jin, Xiaonan Ta. Peroxisome proliferator-activated receptor-gamma activated by ligands can inhibit human lung cancer cell growth through induction of apoptosis. Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban. vol 23. issue 2. 2004-07-22. PMID:12973930. |
to study the expression of peroxisome proliferator-activated receptor-gamma (ppar-gamma) in lung cancer cells, and to testify if the ppar-gamma agonists can inhibit human lung cancer cell growth through induction of apoptosis, ppar-gamma was detected in two lung cancer cell lines by rt-pcr and immunohistochemistry, the inhibition of human lung cancer cell growth was investigated by mtt and cell counts, and the apoptosis was assessed by tunel. |
2004-07-22 |
2023-08-12 |
human |
| Zhongzhi Tang, Ying Tang, Libo F. Growth inhibition and apoptosis induction in human hepatoma cells by tanshinone II A. Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban. vol 23. issue 2. 2004-07-22. PMID:12973939. |
in order to study the effect of tanshinone ii a on growth and apoptosis in human hepatoma cell line bel-7402 in vitro, the human hepatoma cell line bel-7402 was treated with tanshinone ii a at various concentrations for 72 h. growth suppression was evaluated by mtt assay; apoptosis-related alterations in morphology and biochemistry were ascertained under cytochemical staining (hoechst 33258), transmission electron microscopy (tem), and dna agarose gel electrophoresis. |
2004-07-22 |
2023-08-12 |
human |
| Guo-Rui Ruan, Yan-Rong Liu, Shan-Shan Chen, Jia-Yu Fu, Yan Chang, Ya-Zhen Qin, Jin-Lan Li, Hong Yu, Hui Wan. Effect of antisense VEGF cDNA transfection on the growth of chronic myeloid leukemia K562 cells in vitro and in nude mice. Leukemia research. vol 28. issue 7. 2004-07-22. PMID:15158098. |
the growth of transfected cells was investigated by mtt and colony-formation assays, and apoptosis was measured by flow cytometry (fcm) of annexin-v-fitc/pi dual labeled cells. |
2004-07-22 |
2023-08-12 |
mouse |
| Dengju Li, Yaozhen Zhang, Wenjing Cao, Lan Sun, Huizhen Xu, Wu L. Regulative function of telomerase and extracellular regulated protein kinases to leukemic cell apoptosis. Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban. vol 22. issue 4. 2004-07-20. PMID:12674761. |
the proliferation inhibition rate was detected by mtt method, the cell cycle and cell apoptosis was analyzed by flow cytometry and the telomerase activity was detected by the telomeric repeat amplification protocol (trap) assay and bioluminescence analysis method. |
2004-07-20 |
2023-08-12 |
Not clear |
| Zhaohui Zhu, Shian Xing, Chen Lin, Fuqing Zeng, Gongcheng Lu, Ming Fu, Xueyan Zhang, Xiao Liang, Ming W. Adenovirus-mediated transfer of p53 and p16 inhibiting proliferating activity of human bladder cancer cell EJ in vitro and in vivo. Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban. vol 22. issue 4. 2004-07-20. PMID:12674770. |
cell growth, morphological change, cell cycle, apoptosis were measured using mtt assay, flow cytometry, cloning formation, immunocytochemical assays. |
2004-07-20 |
2023-08-12 |
human |
| Hiroshi Yamaguchi, Fumiaki Tanaka, Noriaki Sadanaga, Mitsuhiko Ohta, Hiroshi Inoue, Masaki Mor. Stimulation of CD40 inhibits Fas- or chemotherapy-mediated apoptosis and increases cell motility in human gastric carcinoma cells. International journal of oncology. vol 23. issue 6. 2004-07-20. PMID:14612943. |
in 3-4,5 dimethylthiozol-2-yl-2,5-diphenyl tetrazolium bromide (mtt) assay and annexin v/propidium iodide staining, cd40 stimulation using a soluble form of cd40 ligand did not affect cell viability, but significantly inhibited fas-mediated or chemotherapy-mediated apoptosis in three cd40-positive gastric cancer cell lines. |
2004-07-20 |
2023-08-12 |
human |
| Wanguang Zhang, Xiaoping Chen, Fazu Qi. An antisense plasmid targeting survivin expression induces apoptosis and sensitizes hepatocarcinoma cells to chemotherapy. Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban. vol 23. issue 4. 2004-07-20. PMID:15015643. |
survivin protein was detected by western-blot, the growth activity was measured by mtt, and apoptosis was detected by flow cytometry 12 h, 24 h, 48 h after transfection. |
2004-07-20 |
2023-08-12 |
Not clear |
| C B S Lau, C Y Ho, C F Kim, K N Leung, K P Fung, T F Tse, H H L Chan, M S S Cho. Cytotoxic activities of Coriolus versicolor (Yunzhi) extract on human leukemia and lymphoma cells by induction of apoptosis. Life sciences. vol 75. issue 7. 2004-07-20. PMID:15183073. |
hence, the objective of this study was to examine the in vitro cytotoxic activities of a standardized aqueous ethanol extract prepared from coriolus versicolor on a b-cell lymphoma (raji) and two human promyelocytic leukemia (hl-60, nb-4) cell lines using a mtt cytotoxicity assay, and to test whether the mechanism involves induction of apoptosis. |
2004-07-20 |
2023-08-12 |
human |
| Shing-Chuan Shen, Ching Huai Ko, Shi-Wen Tseng, Shu-Huei Tsai, Yen-Chou Che. Structurally related antitumor effects of flavanones in vitro and in vivo: involvement of caspase 3 activation, p21 gene expression, and reactive oxygen species production. Toxicology and applied pharmacology. vol 197. issue 2. 2004-07-13. PMID:15163544. |
results of the mtt assay indicate that 2'-oh flavanone showed the most potent cytotoxic effect on these three cells, and cell death induced by 2'-oh flavanone was via the occurrence of dna ladders, apoptotic bodies, and hypodiploid cells, all characteristics of apoptosis. |
2004-07-13 |
2023-08-12 |
mouse |
| Jun Yang, Scott F Gallagher, Krista Haines, P K Epling-Burnette, Fenqi Bai, William R Gower, Stephen Mastorides, James G Norman, Michel M Mur. Kupffer cell-derived Fas ligand plays a role in liver injury and hepatocyte death. Journal of gastrointestinal surgery : official journal of the Society for Surgery of the Alimentary Tract. vol 8. issue 2. 2004-07-01. PMID:15036192. |
hepatocytes were treated with fasl and their viability was assessed by monotetrazolium (mtt), apoptosis by flow cytometry, as well as caspase-3 and p38-mitogen-activated protein kinase (mapk) by immunoblotting. |
2004-07-01 |
2023-08-12 |
Not clear |
| Marina Ioudina, Etsuro Uemura, Heather West Greenle. Glucose insufficiency alters neuronal viability and increases susceptibility to glutamate toxicity. Brain research. vol 1004. issue 1-2. 2004-06-24. PMID:15033434. |
we investigated the effect of a restricted supply of glucose on neuronal vulnerability to glutamate by assaying cellular atp levels (cellular energy production), 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (mtt) reduction (mitochondrial function), lactate dehydrogenase (ldh) release (cellular viability) and activation of caspase-3 (apoptosis) in rat hippocampal neurons cultured in media (1.7, 5 and 25 mm glucose) with or without 100 microm glutamate. |
2004-06-24 |
2023-08-12 |
rat |
| Haitao Wang, Xiangliang Yang, Zhihong Zhang, Huibi X. Both calcium and ROS as common signals mediate Na(2)SeO(3)-induced apoptosis in SW480 human colonic carcinoma cells. Journal of inorganic biochemistry. vol 97. issue 2. 2004-06-22. PMID:14512201. |
when sw480 cells were exposed to 25-100 microm na(2)seo(3), both cell apoptosis and growth inhibition were observed by flow cytometric analysis and 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (mtt) assay. |
2004-06-22 |
2023-08-12 |
human |
| Kostas V Floros, Hellinida Thomadaki, George Lallas, Nikos Katsaros, Maroulio Talieri, Andreas Scorila. Cisplatin-induced apoptosis in HL-60 human promyelocytic leukemia cells: differential expression of BCL2 and novel apoptosis-related gene BCL2L12. Annals of the New York Academy of Sciences. vol 1010. 2004-06-15. PMID:15033711. |
the kinetics of apoptosis induction and cell toxicity were evaluated by dna laddering and the mtt method, respectively. |
2004-06-15 |
2023-08-12 |
human |
| Ryungsa Kim, Kazuaki Tanabe, Manabu Emi, Yoko Uchida, Tetsuya Tog. Death receptor-dependent and -independent pathways in anticancer drug-induced apoptosis of breast cancer cells. Oncology reports. vol 10. issue 6. 2004-06-10. PMID:14534720. |
drug sensitivity and apoptosis were assessed by mtt assay and tunel, respectively. |
2004-06-10 |
2023-08-12 |
Not clear |
| Ailing Li, Hongwei Li, Jing Zhang, Gang Jin, Ruijuan Xi. The mitogenic and anti-apoptotic activity of tumor conditioned medium on endothelium. Clinical hemorheology and microcirculation. vol 29. issue 3-4. 2004-05-19. PMID:14724364. |
huvecs were exposed to tcm from breast carcinoma cell line mda-mb-231, then we measured their proliferation, apoptosis and cell cycle distribution by mtt and flow cytometery (fcm). |
2004-05-19 |
2023-08-12 |
human |