| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Oskar S Frankfurt, Awtar Krisha. Apoptosis enzyme-linked immunosorbent assay distinguishes anticancer drugs from toxic chemicals and predicts drug synergism. Chemico-biological interactions. vol 145. issue 1. 2003-05-09. PMID:12606157. |
short-term metabolic tetrazolium-based 3-(4,5-dimethylthiazol-yl)-2,5-diphenyformazan bromide (mtt) assay was significantly less sensitive than apoptosis elisa and the cell survival assay. |
2003-05-09 |
2023-08-12 |
Not clear |
| Jeong-Jin Je, Hyun-Taeg Shin, Seok-Hee Chung, Jong-Soo Lee, Sung-Soo Kim, Hyun-Dae Shin, Mi-Hyeon Jang, Youn-Jung Kim, Joo-Ho Chung, Ee-Hwa Kim, Chang-Ju Ki. Protective effects of Wuyaoshunqisan against H2O2-induced apoptosis on hippocampal cell line HiB5. The American journal of Chinese medicine. vol 30. issue 4. 2003-05-07. PMID:12568283. |
to investigate the protective effects of wuyaoshunqisan against h2o2-induced apoptosis in the central nervous system, the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (mtt) assay, the terminal deoxynucleotidyl transferase (tdt)-mediated dutp nick end-labeling (tunel) method, flow cytometric analysis, and the dna fragmentation assay were performed on cells of the hippocampal cell line hib5. |
2003-05-07 |
2023-08-12 |
Not clear |
| Xiang-Dong Gao, Wen-Cai Ye, Albert C H Yu, Yun Zhang, Ren-Xiang Tan, Ming Li, W L Wendy Hsia. Pulsatilloside A and anemoside A3 protect PC12 cells from apoptosis induced by sodium cyanide and glucose deprivation. Planta medica. vol 69. issue 2. 2003-05-07. PMID:12624827. |
additions of pulsatilloside a and anemoside a 3, at dosages ranging from 0.1, 1 and 10 microg/ml, protected pc12 cells from apoptosis determined by mtt, ldh release analysis, and flow cytometry measurement. |
2003-05-07 |
2023-08-12 |
Not clear |
| C G Yuan, L He, X L Xue, Y T Shi, X H B. [Effect of alpha-lipoic acid on the apoptosis of PC12 cells induced by 6-hydroxydopamine]. Shi yan sheng wu xue bao. vol 34. issue 1. 2003-05-06. PMID:12549012. |
it was confirmed by the different percentage of cell survival rate and apoptosis concluded from fcm and mtt. |
2003-05-06 |
2023-08-12 |
rat |
| C G Yuan, L He, X L Xue, Y T Shi, X H B. [Effect of alpha-lipoic acid on the apoptosis of PC12 cells induced by 6-hydroxydopamine]. Shi yan sheng wu xue bao. vol 34. issue 1. 2003-05-06. PMID:12549012. |
the result indicateed that alpha-lipoic acid can partly prevent apoptosis induced by 6-ohda but fail to prevent necrosis since it can decrease the apoptotic cell from 20.09% to 3.09%, just as increased cell survival rate from 56.8% to 72.6% but can not reach the normal level showed by mtt assay. |
2003-05-06 |
2023-08-12 |
rat |
| Mi-Hyeon Jang, Min-Chul Shin, In-Sug Kang, Hyung-Hwan Baik, Yong-Ho Cho, Jong-Phill Chu, Ee-Hwa Kim, Chang-Ju Ki. Caffeine induces apoptosis in human neuroblastoma cell line SK-N-MC. Journal of Korean medical science. vol 17. issue 5. 2003-05-01. PMID:12378022. |
to investigate whether caffeine induces apoptosis in the central nervous system, 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (mtt) assay, 4,6-diamidino-2-phenylindole (dapi) staining, terminal deoxynucleotidyl transferase (tdt)-mediated dutp nick end labeling (tunel) assay, flow cytometric analysis, dna fragmentation assay, and caspase-3 enzyme assay were performed on sk-n-mc human neuroblastoma cells. |
2003-05-01 |
2023-08-12 |
human |
| Akiko Tabuchi, Kouhei Funaji, Jun Nakatsubo, Mamoru Fukuchi, Tomofusa Tsuchiya, Masaaki Tsud. Inactivation of aconitase during the apoptosis of mouse cerebellar granule neurons induced by a deprivation of membrane depolarization. Journal of neuroscience research. vol 71. issue 4. 2003-04-01. PMID:12548706. |
suppression of the apoptosis of cgcs mediated by the ca(2+) influx or neurotrophic factors such as brain-derived neurotrophic factor (bdnf) and adenylate cyclase activating polypeptide-38 (pacap-38) attenuated the aconitase inactivation as well as the lactate dehydrogenase (ldh)-release and the decrease in mtt reduction. |
2003-04-01 |
2023-08-12 |
mouse |
| Mi-Hyeon Jang, Min-Chul Shin, Hye-Sook Shin, Khae-Hawn Kim, Heung-Jae Park, Ee-Hwa Kim, Chang-Ju Ki. Alcohol induces apoptosis in TM3 mouse Leydig cells via bax-dependent caspase-3 activation. European journal of pharmacology. vol 449. issue 1-2. 2003-03-20. PMID:12163104. |
to investigate whether ethanol induces apoptosis in leydig cells, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (mtt) assay, terminal deoxynuclotidyl transferase-mediated dutp nick end-labeling (tunel) assay, 4,6-diamidino-2-phenylindole (dapi) staining, dna fragmentation assay, caspase-3 enzyme assay, reverse transcription-polymerase chain reaction (rt-pcr) and western blot analysis were performed on tm3 mouse leydig cells. |
2003-03-20 |
2023-08-12 |
mouse |
| A L McKeague, D J Wilson, John Nelso. Staurosporine-induced apoptosis and hydrogen peroxide-induced necrosis in two human breast cell lines. British journal of cancer. vol 88. issue 1. 2003-03-14. PMID:12556971. |
staurosporine (ssp)-induced apoptosis and h(2)o(2)-induced necrosis were evaluated by two cytotoxicity assays, neutral red (nr) and methyl-thiazolyl tertrazolium (mtt), in comparison with a differential dye uptake assay, using hoechst33342/propidium iodide (hoechst/pi). |
2003-03-14 |
2023-08-12 |
human |
| A L McKeague, D J Wilson, John Nelso. Staurosporine-induced apoptosis and hydrogen peroxide-induced necrosis in two human breast cell lines. British journal of cancer. vol 88. issue 1. 2003-03-14. PMID:12556971. |
results also showed that neither mtt or nr can distinguish between the modes of cell death, nor detect the early onset of apoptosis revealed by hoechst/pi. |
2003-03-14 |
2023-08-12 |
human |
| Lu Zuo, Jian Li, Ti Shen, Zhi-Nan Zhan. [The comparison between the mechanisms of sodium selenite induced apoptosis and arsenic trioxide induced apoptosis in human acute promyelocytic leukemia cell line NB4 cells]. Zhongguo shi yan xue ye xue za zhi. vol 10. issue 3. 2003-03-10. PMID:12513784. |
in order to explore the differences between the mechanisms of selenite-induced apoptosis and arsenic induced apoptosis in nb4 cells, growth inhibition was determined by mtt test, apoptosis determined by dna electrophoresis and analysis of intracellular dna contents, reactive oxygen species and reduced glutathione in the cell were measured by lucigenin dependent chemoluminescent (cl) test and spectrophotometry, and mitochondrial transmembrane potential was measured by flow cytometry. |
2003-03-10 |
2023-08-12 |
human |
| Hui Yang, Shen-Wu Wang, Hui-Jun Yi. [p21(WAF1) transfection decreases sensitivity of K562 cells to VP-16]. Zhongguo shi yan xue ye xue za zhi. vol 10. issue 1. 2003-02-03. PMID:12513833. |
the k562-p21(waf1) cells were exposed to vp-16 at different concentrations and different times, then, the sensitivity to vp-16 was examined by cell viability and mtt assay, the apoptosis induced by vp-16 was examined by dna fragments electrophoresis and flow cytometric annexin v-pi dual labeling technique. |
2003-02-03 |
2023-08-12 |
Not clear |
| Gabriella Lupo, Giovanna Assero, Carmelina D Anfuso, Ambra Nicotra, Maddalena Palumbo, Giuseppe Cannavò, Marcella Renis, Nicola Ragusa, Mario Alberghin. Cytosolic phospholipase A2 mediates arachidonoyl phospholipid hydrolysis in immortalized rat brain endothelial cells stimulated by oxidized LDL. Biochimica et biophysica acta. vol 1585. issue 1. 2003-01-08. PMID:12457711. |
cell lysates and subcellular fractions were assayed for cpla(2) while the cytotoxic effect and apoptosis were monitored by morphological changes, trypan blue dye exclusion, mtt reduction test, caspase-3 activity, comet and laser confocal fluorescence microscopy (lcfm) analyses. |
2003-01-08 |
2023-08-12 |
rat |
| Rui F M Silva, Cecília M P Rodrigues, Dora Brite. Rat cultured neuronal and glial cells respond differently to toxicity of unconjugated bilirubin. Pediatric research. vol 51. issue 4. 2002-11-26. PMID:11919342. |
ucb toxicity was evaluated by lactate dehydrogenase release, induction of apoptosis, cytoskeleton degeneration, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (mtt) reduction, and glutamate uptake. |
2002-11-26 |
2023-08-12 |
rat |
| Rui F M Silva, Cecília M P Rodrigues, Dora Brite. Rat cultured neuronal and glial cells respond differently to toxicity of unconjugated bilirubin. Pediatric research. vol 51. issue 4. 2002-11-26. PMID:11919342. |
in conclusion, the study demonstrates that astrocytes are more susceptible to inhibition of glutamate uptake and mtt reduction by ucb, whereas neurons are more sensitive to cell death by necrosis or apoptosis. |
2002-11-26 |
2023-08-12 |
rat |
| Chun-Qiong Feng, Wen-Li Ma, Yan-Bing Song, Qiu-Ye Guo, Qing-Hua Wu, Wen-Ling Zhen. [Detection of cell apoptosis by MTT assay]. Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA. vol 22. issue 3. 2002-11-25. PMID:12390784. |
[detection of cell apoptosis by mtt assay]. |
2002-11-25 |
2023-08-12 |
Not clear |
| Chun-Qiong Feng, Wen-Li Ma, Yan-Bing Song, Qiu-Ye Guo, Qing-Hua Wu, Wen-Ling Zhen. [Detection of cell apoptosis by MTT assay]. Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA. vol 22. issue 3. 2002-11-25. PMID:12390784. |
to study the feasibility of using mtt assay to detect cell apoptosis. |
2002-11-25 |
2023-08-12 |
Not clear |
| Su-Min Chi, Cheng-Xin Li, Ya-Li Liu, Yun-Long Zhu, Jian-Wen Gu, Liang Du, Fu-Zhou Wan. [The effect of acetylcholine on the proliferation and apoptosis of three kinds of cultured human pituitary adenoma cells]. Sheng li xue bao : [Acta physiologica Sinica]. vol 54. issue 3. 2002-11-22. PMID:12075474. |
in order to elucidate the effect of acetylcholine (ach) on the occurrence and development of human pituitary adenoma, it was firstly observed whether there exists choline acetyl transferase (chat) which is necessary for the synthesis of acetylcholine in the cells of human pituitary adenoma, and then mtt method, (3)h tdr incorporation, cell cycle analysis and tunel were employed to estimate the influence of ach on the proliferation, dna synthesis and apoptosis of three kinds of human pituitary adenoma (human prolactinoma, somatotropinoma and non-functional tumor) cells cultured in vitro. |
2002-11-22 |
2023-08-12 |
human |
| X L Chen, S S Huang, Y X Yao, W B Li, X L Wang, A M Zho. Promotion of pulmonary fibroblast proliferation and apoptosis by sodium nitroprusside. Sheng li xue bao : [Acta physiologica Sinica]. vol 53. issue 6. 2002-11-19. PMID:11930231. |
the effects of nitric oxide (no) donor sodium nitroprusside (snp) on the proliferation and apoptosis and on bcl-2, bax and p53 proteins of pulmonary fibroblasts were investigated by using mtt cleavage assay, agarose gel electrophoresis and flow cytometric analysis. |
2002-11-19 |
2023-08-12 |
Not clear |
| Joon Song, Thomas Rutherford, Frederick Naftolin, Santiago Brown, Gil Mo. Hormonal regulation of apoptosis and the Fas and Fas ligand system in human endometrial cells. Molecular human reproduction. vol 8. issue 5. 2002-10-30. PMID:11994542. |
apoptosis was determined by 3-[4,5-dimethylthiazol-2-yl]-2,5-dephenyl tetrazolium bromide (mtt) assay and propidium iodide staining, while fas and fasl expression were evaluated by western blot analysis. |
2002-10-30 |
2023-08-12 |
human |