| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| J Kumi-Diak. Chemosensitivity of human prostate cancer cells PC3 and LNCaP to genistein isoflavone and beta-lapachone. Biology of the cell. vol 94. issue 1. 2002-10-28. PMID:12000145. |
in this study the combined effects of genistein and beta-lapachone on the chemosensitivity of lncap and pc3 human prostate cancer cells was determined in vitro, using 3-[4,5-dimethylthiazol-2-yl]-2-,5-diphenyltetrazolium bromide (mtt) and lactate dehydrogenase (ldh) to study treatment-induced growth inhibition and cytotoxicity and, annexin v-fluoresceine (fi) and terminal deoxyribonucleotidyl transferase-mediated dutp nick-end labeling (tunel)-propidium iodide (pi) assays to determine potential treatment-induced apoptosis and/or necrosis. |
2002-10-28 |
2023-08-12 |
human |
| Michael André Kern, Dominic Schubert, Dina Sahi, Mirja Mareike Schöneweiss, Ilona Moll, Anke Maria Haugg, Hans Peter Dienes, Kai Breuhahn, Peter Schirmache. Proapoptotic and antiproliferative potential of selective cyclooxygenase-2 inhibitors in human liver tumor cells. Hepatology (Baltimore, Md.). vol 36. issue 4 Pt 1. 2002-10-24. PMID:12297835. |
the functional effects of the nonselective inhibitor sulindac sulfide and the cox-2 selective inhibitors sc-58635 and meloxicam were examined by 3(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazoliumbromide (mtt)-assays and brdu uptake, morphology, and tunel analysis of apoptosis. |
2002-10-24 |
2023-08-12 |
human |
| X Luan, T G H Diekwisc. CP27 affects viability, proliferation, attachment and gene expression in embryonic fibroblasts. Cell proliferation. vol 35. issue 4. 2002-09-13. PMID:12153613. |
following cell culture, cell viability, apoptosis, cell proliferation, cell shape, cellular attachment and fibronectin matrix production were assayed using mtt colourimetric assay, brdu staining, morphometry, immunostaining and western blot analysis. |
2002-09-13 |
2023-08-12 |
rabbit |
| Angela M Djanani, Ch M Kähle. Modulation of inflammation by vasoactive intestinal peptide and bombesin: lack of effects on neutrophil apoptosis. Acta medica Austriaca. vol 29. issue 3. 2002-09-11. PMID:12168570. |
for measuring effects on cell survival and apoptosis, trypan dye exclusion, colorimetric mtt assay to assess cell survival, and caspase-3 assay and annexin-v binding for analysing apoptosis rates were used. |
2002-09-11 |
2023-08-12 |
human |
| Ferenc Hollósy, Tamás Lóránd, László Orfi, Dániel Erös, György Kéri, Miklós Ide. Relationship between lipophilicity and antitumor activity of molecule library of Mannich ketones determined by high-performance liquid chromatography, clogP calculation and cytotoxicity test. Journal of chromatography. B, Analytical technologies in the biomedical and life sciences. vol 768. issue 2. 2002-08-29. PMID:11888064. |
compounds were tested on their ability to inhibit the proliferation of the a431 human adenocarcinoma cell line evaluated by mtt and apoptosis assays. |
2002-08-29 |
2023-08-12 |
human |
| Ming-Jen Hsu, Shiuh-Sheng Lee, Wan-Wan Li. Polysaccharide purified from Ganoderma lucidum inhibits spontaneous and Fas-mediated apoptosis in human neutrophils through activation of the phosphatidylinositol 3 kinase/Akt signaling pathway. Journal of leukocyte biology. vol 72. issue 1. 2002-08-12. PMID:12101282. |
annexin v staining and mtt assays reveal that ps-g is able to inhibit spontaneous and fas-induced neutrophil apoptosis, and this effect of ps-g is enhanced by the presence of zvad (a caspase inhibitor) and gm-csf. |
2002-08-12 |
2023-08-12 |
human |
| V N Komarevtsev, I A Komarevtseva, D A Fil'chukov, N N Golovchenko, V V Kharchenk. [Apoptosis and pathomorphology of renal tumors]. Likars'ka sprava. issue 4. 2002-05-30. PMID:11692689. |
the degree of dna fragmentation in a biopsy specimen proved to be of value as a guide to detection of apoptosis; the activity of proliferation was studied by the mtt method using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide. |
2002-05-30 |
2023-08-12 |
Not clear |
| Masafumi Takahashi, Hitoaki Okazaki, Yukiyo Ogata, Koichi Takeuchi, Uichi Ikeda, Kazuyuki Shimad. Lysophosphatidylcholine induces apoptosis in human endothelial cells through a p38-mitogen-activated protein kinase-dependent mechanism. Atherosclerosis. vol 161. issue 2. 2002-05-14. PMID:11888522. |
the presence of apoptosis was evaluated by morphological criteria, mtt assay, and electrophoresis of dna fragments showing the characteristic apoptotic ladder, tunel analysis, and quantified as the proportion of hypodiploid cells by flow cytometry. |
2002-05-14 |
2023-08-12 |
human |
| M F Lucero Gritti, M Beviacqua, R H Bordenave, L S Rum. Interferon-alpha 2b modulation of doxorubicin sensitivity in a multidrug resistant cell line. Journal of experimental & clinical cancer research : CR. vol 20. issue 3. 2002-05-02. PMID:11718220. |
dox 50 ng/ml - ifn alpha 2b 500 iu/ml treatment inhibited cell proliferation (47.2 +/- 1.4%, p < 0.0001; mtt assay: 40.6 +/- 1.2%, p < 0.0001) and augmented the expression of p-170, bcl-2 and hla-abc, while it didn't exert apoptosis, producing a slight g2/m arrest. |
2002-05-02 |
2023-08-12 |
Not clear |
| Tatiana Dilla, Jesús Romero, Pilar Sanstisteban, Juan A Velasc. The mdm2 proto-oncogene sensitizes human medullary thyroid carcinoma cells to ionizing radiation. Oncogene. vol 21. issue 15. 2002-04-29. PMID:11948421. |
mtt cells expressing mdm2 (mtt-mdm2) are unable to respond to dna damage with g2m arrest, and display a high percentage of apoptosis. |
2002-04-29 |
2023-08-12 |
human |
| Cecilia Anselmi, Anna Ettorre, Marco Andreassi, Marisanna Centini, Paolo Neri, Anna Di Stefan. In vitro induction of apoptosis vs. necrosis by widely used preservatives: 2-phenoxyethanol, a mixture of isothiazolinones, imidazolidinyl urea and 1,2-pentanediol. Biochemical pharmacology. vol 63. issue 3. 2002-04-25. PMID:11853695. |
using hl60 cells, we monitored the kinetics of cell toxicity with the mtt test and analysed extranuclear end points of apoptosis, i.e. |
2002-04-25 |
2023-08-12 |
Not clear |
| Mohamed M Rafi, Bret C Vastano, Nanquan Zhu, Chi-Tang Ho, Geetha Ghai, Robert T Rosen, Michael A Gallo, Robert S DiPaol. Novel polyphenol molecule isolated from licorice root (Glycrrhiza glabra) induces apoptosis, G2/M cell cycle arrest, and Bcl-2 phosphorylation in tumor cell lines. Journal of agricultural and food chemistry. vol 50. issue 4. 2002-04-23. PMID:11829627. |
pure beta-hydroxy-dhp induced bcl-2 phosphorylation in breast and prostate tumor cells, g2/m cell cycle arrest, apoptosis demonstrated by annexin v and tunel assay, decreased cell viability demonstrated by a tetrazolium (mtt) assay, and altered microtubule structure. |
2002-04-23 |
2023-08-12 |
Not clear |
| Enrico Lucarelli, Luca Sangiorgi, Veronica Maini, Giovanna Lattanzi, Sandra Marmiroli, Matteo Reggiani, Marina Mordenti, Giuliana Alessandra Gobbi, Francesca Scrimieri, Annarosa Zambon Bertoja, Piero Picc. Troglitazione affects survival of human osteosarcoma cells. International journal of cancer. vol 98. issue 3. 2002-04-16. PMID:11920584. |
in our experiments, tro never induced apoptosis of os cells; on the contrary, tro increased cell number, based on mtt proliferation assay. |
2002-04-16 |
2023-08-12 |
human |
| Z Z Guan, H Miao, J Y Tian, C Unger, A Nordberg, X Zhan. Suppressed expression of nicotinic acetylcholine receptors by nanomolar beta-amyloid peptides in PC12 cells. Journal of neural transmission (Vienna, Austria : 1996). vol 108. issue 12. 2002-03-07. PMID:11810405. |
abeta1-40 and abeta25-35 at the concentrations used in the treatment study neither bound to nachrs nor induced apoptosis, but significantly inhibited the mtt (3-(4,5-dimethylthiazol-2-yl)-2,5,diphenyl tetrazolium bromide) reduction. |
2002-03-07 |
2023-08-12 |
Not clear |
| Z Gao, H Matsuo, Y Wang, S Nakago, T Maru. Up-regulation by IGF-I of proliferating cell nuclear antigen and Bcl-2 protein expression in human uterine leiomyoma cells. The Journal of clinical endocrinology and metabolism. vol 86. issue 11. 2001-12-13. PMID:11701740. |
the effects of igf-i on bcl-2 protein and pcna expression in cultured leiomyoma cells were assessed by western immunoblot analysis and immunocytochemical staining, whereas the effects of igf-i on the cell viability and apoptosis of the cultured cells were determined by 3-(4,5-dimethylatriazol-2-yl)-2,5diphenyltetrasodium bromide (mtt) assay and terminal deoxynucleotidyl transferase-mediated 2'-deoxyuridine 5'-triphosphate nick end labeling assay, respectively. |
2001-12-13 |
2023-08-12 |
human |
| L Weinrib, J H Li, J Donovan, D Huang, F F Li. Cisplatin chemotherapy plus adenoviral p53 gene therapy in EBV-positive and -negative nasopharyngeal carcinoma. Cancer gene therapy. vol 8. issue 5. 2001-09-27. PMID:11477455. |
clonogenic and mtt assays were used to assess the sensitivity of cells to these treatments, and apoptosis was also quantified. |
2001-09-27 |
2023-08-12 |
human |
| Y Muneta, Y Shimoji, S Inumaru, Y Mor. Molecular cloning, characterization, and expression of porcine Fas ligand (CD95 ligand). Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research. vol 21. issue 5. 2001-08-23. PMID:11429161. |
the recombinant porcine fasl expressed by recombinant baculovirus containing the whole coding sequences of porcine fasl showed cytotoxic effect and induced apoptosis in porcine renal tubular cell line pk-15 cells sensitized by cycloheximide (chx), which was confirmed by mtt assay, dna fragmentation assay, and tunel staining, respectively. |
2001-08-23 |
2023-08-12 |
human |
| E Lee, S H Jeon, J Y Yi, Y J Jin, Y S So. Calcipotriol inhibits autocrine phosphorylation of EGF receptor in a calcium-dependent manner, a possible mechanism for its inhibition of cell proliferation and stimulation of cell differentiation. Biochemical and biophysical research communications. vol 284. issue 2. 2001-07-12. PMID:11394895. |
based on mtt assay, pcna staining, dapi staining, and involucrin immunocytochemical staining, we showed that calcipotriol inhibited cell growth and stimulated differentiation but did not induce apoptosis. |
2001-07-12 |
2023-08-12 |
Not clear |
| B Montaner, R Pérez-Tomá. Prodigiosin-induced apoptosis in human colon cancer cells. Life sciences. vol 68. issue 17. 2001-06-14. PMID:11388704. |
dld-1 and sw-620 human colon adenocarcinoma cells, nrk and swiss-3t3 nonmalignant cells were assayed by the mtt assay, fragmentation pattern of dna, hoechst 33342 staining and study of parp cleavage by western blot, in order to characterize the prodigiosin-induced apoptosis. |
2001-06-14 |
2023-08-12 |
human |
| S Yokoyama, H Hirano, N Wakimaru, K P Sarker, J Kurats. Inhibitory effect of epigallocatechin-gallate on brain tumor cell lines in vitro. Neuro-oncology. vol 3. issue 1. 2001-05-31. PMID:11305413. |
cell viability was determined by assay with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (mtt), and the extent of apoptosis was studied by flow cytometric analysis. |
2001-05-31 |
2023-08-12 |
human |