| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Mi-Hyeon Jang, Min-Chul Shin, Youn-Jung Kim, Chang-Ju Kim, Joo-Ho Chung, Jung-Chul Seo, Ee-Hwa Kim, Kyu-Yeol Kim, Choong-Yeol Lee, Kyeong-Mi Ki. Protective effect of Acanthopanax senticosus against ethanol-induced apoptosis of human neuroblastoma cell line SK-N-MC. The American journal of Chinese medicine. vol 31. issue 3. 2004-01-13. PMID:12943169. |
the protective effect of acanthopanax senticosus (as) against ethanol (etoh)-induced apoptosis of the human neuroblastoma cell line sk-n-mc was investigated via 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (mtt) assay, flow cytometric analysis, dna fragmentation assay, reverse transcription-polymerase chain reaction (rt-pcr), and caspase-3 assay. |
2004-01-13 |
2023-08-12 |
human |
| Rebecca L Uzarski, Zahidul Islam, James J Pestk. Potentiation of trichothecene-induced leukocyte cytotoxicity and apoptosis by TNF-alpha and Fas activation. Chemico-biological interactions. vol 146. issue 2. 2003-12-16. PMID:14597125. |
cytotoxicity and apoptosis were evaluated by mtt assay and morphologic assays, respectively. |
2003-12-16 |
2023-08-12 |
Not clear |
| Jae Joon Lee, Yu Mi Kim, Shou Yu Yin, Hee Dong Park, Min Hee Kang, Jin Tae Hong, Myung Koo Le. Aggravation of L-DOPA-induced neurotoxicity by tetrahydropapaveroline in PC12 cells. Biochemical pharmacology. vol 66. issue 9. 2003-11-21. PMID:14563489. |
exposure of pc12 cells up to 10 microm thp or 20 microm l-dopa after 24 or 48 hr, neither affected the cell viability determined by mtt assay, nor induced apoptosis by flow cytometry and tunel staining. |
2003-11-21 |
2023-08-12 |
rat |
| Shing-Chuan Shen, Yen-Chou Chen, Feng-Lin Hsu, Woan-Rouh Le. Differential apoptosis-inducing effect of quercetin and its glycosides in human promyeloleukemic HL-60 cells by alternative activation of the caspase 3 cascade. Journal of cellular biochemistry. vol 89. issue 5. 2003-11-18. PMID:12874837. |
results of the mtt assay showed that que, but not rut and qui, exhibits significant cytotoxic effect on hl-60 cells, accompanied by the dose- and time-dependent appearance of characteristics of apoptosis including an increase in dna ladder intensity, morphological changes, apoptotic bodies, and an increase in hypodiploid cells by flow cytometry analysis. |
2003-11-18 |
2023-08-12 |
human |
| Yen-Chou Chen, Shing-Chuan Shen, Hui-Yi Li. Rutinoside at C7 attenuates the apoptosis-inducing activity of flavonoids. Biochemical pharmacology. vol 66. issue 7. 2003-10-24. PMID:14505793. |
results of the mtt assay showed that ht and ne, but not hd and ni, exhibited significant cytotoxic effect in hl-60 cells, accompanied by the dose- and time-dependent appearance of characteristics of apoptosis including an increase in dna ladder intensity, morphological changes, appearance of apoptotic bodies, and an increase in hypodiploid cells by flow cytometry analysis. |
2003-10-24 |
2023-08-12 |
human |
| Xiao-Yong Lei, Yuan Zhan. [Design of antisense oligodeoxynucleotide targeting at bcl-2 mRNA and observation on its effect on the sensitivity of leukemia cells to arabinosyl cytosine]. Zhongguo shi yan xue ye xue za zhi. vol 11. issue 1. 2003-10-09. PMID:12667288. |
the median inhibitory concentration (ic(50)) of ara-c for hl-60 and k562 cells was determined with mtt method; the apoptosis rate and bcl-2 protein expression level were assayed by flow cytometry. |
2003-10-09 |
2023-08-12 |
Not clear |
| Ze-Lin Liu, Zuo-Ren Dong, Xue-Jun Zhang, Fu-Xu Wang, Jing-Ci Yang, Wei-Dong Ma, Xing-Yan Du, Li Ya. [Effects of aminophylline on proliferation and apoptosis in Raji lympho-blastoid cell line]. Zhongguo shi yan xue ye xue za zhi. vol 11. issue 1. 2003-10-09. PMID:12667289. |
mtt assay, light and transmission electron microscopy and annexin v staining were used to observe cell proliferation, morphologic changes and apoptosis rate in am-treated cells, and fcm and rt-pcr techniques were adopted to detect the effect on cell cycle, the expression of cyclin b1 and bcl-2 and mitochondrial transmembrane potential in am-treated cells. |
2003-10-09 |
2023-08-12 |
Not clear |
| Claudia A Vosseler, Wolfgang Erl, Peter C Webe. Structural requirements of cyclopentenone prostaglandins to induce endothelial cell apoptosis. Biochemical and biophysical research communications. vol 307. issue 2. 2003-09-16. PMID:12859958. |
cell viability was tested with a modified mtt assay and apoptosis was detected by annexin v staining and cell cycle analysis by flow cytometry. |
2003-09-16 |
2023-08-12 |
human |
| Shinji Matsumoto, Satoshi Yamamoto, Katsunari Sai, Keishi Maruo, Masaru Adachi, Masaru Saitoh, Tomoyuki Nishizak. Pipecolic acid induces apoptosis in neuronal cells. Brain research. vol 980. issue 2. 2003-09-16. PMID:12867256. |
notably, 72-h treatment with d-, l-, and dl-pipecolic acid reduced neuro-2a cell viability in a dose-dependent manner at concentrations ranging from 1 to 100 microm in a 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2h-tetrazolium bromide (mtt) assay, in parallel with reactions to propidium iodide, a marker of cell death, and hoechst 33,342, a marker of apoptosis in a fluorescent microscopic study, with dl-pipecolic acid being the most potent. |
2003-09-16 |
2023-08-12 |
mouse |
| Jingyuan Chen, Yaoming Chen, Wenjing Luo, Xiuhong Li. [Toxicity effects of manganese on PC12 cells]. Wei sheng yan jiu = Journal of hygiene research. vol 31. issue 4. 2003-08-21. PMID:12600022. |
mtt test, trypan blue exclusion test, malondiadehyde(mda) colorimetry and dna gel electrophoresis were performed to exam proliferation, lipid peroxidation, and apoptosis of pc12 cells. |
2003-08-21 |
2023-08-12 |
Not clear |
| Jian Li, Lu Zuo, Ti Shen, Zhi-Nan Zhan. [Inhibition of the activation of transcriptional factor NFkappaB during sodium selenite-induced apoptosis in NB4 cells]. Zhongguo shi yan xue ye xue za zhi. vol 10. issue 5. 2003-08-12. PMID:12513738. |
in order to evaluate the effect of sodium selenite on the activation of nfkappab during selenite-induced apoptosis in nb4 cells, western blot was used to measure the level of p65 in nuclear extraction of nb4 cells treated with sodium selenite to reflect the activation of nfkappab; the apoptosis of nb4 cells was determined by morphological observation, dna ladder electrophoresis and flow cytometry; and mtt test was used to measure the growth inhibition of cells. |
2003-08-12 |
2023-08-12 |
Not clear |
| Mark Arundine, Teresa Sanelli, Bei Ping He, Michael J Stron. NMDA induces NOS 1 translocation to the cell membrane in NGF-differentiated PC 12 cells. Brain research. vol 976. issue 2. 2003-07-28. PMID:12763249. |
although no evidence of cytotoxicity was observed utilizing either the mtt assay or measures of apoptosis within the maximal interval of nos1 translocation, cell viability was reduced following 10 h of continuous nmda exposure. |
2003-07-28 |
2023-08-12 |
Not clear |
| Deng-Ju Li, Yao-Zhen Zhang, Fan-Kai Meng, Dong-Hua Zhang, Wen-Li Li. Regulative function of extracellular regulated protein kinases and telomerase in apoptosis of hepatocarcinomatous and leukemic cell lines. Zhongguo shi yan xue ye xue za zhi. vol 10. issue 4. 2003-07-23. PMID:12513760. |
in order to investigate the change of telomerase activity and phosphorylated (activated) extracellular regulated protein kinases (erk) 1 and 2 in hepatocarcinomatous cell line smmc7721 and leukemic cell line k562 proliferation inhibition and apoptosis, three kinds of chemotherapeutic drugs harringtonine (hrt), vincristine (vcr) and etoposide (vp-16) were selected as inducers; and mtt assay, flow cytometry analysis, telomeric repeat amplification protocol (trap) assay and bioluminescence analysis were used. |
2003-07-23 |
2023-08-12 |
Not clear |
| Ze-Lin Liu, Zuo-Ren Dong, Fu-Xu Wang, Xue-Jun Zhang, Jing-Ci Yang, Wei-Dong Ma, Xing-Yan Du, Li Ya. [Effects of sodium orthovanadate on proliferation and apoptosis in raji cells and its mechanism]. Zhongguo shi yan xue ye xue za zhi. vol 10. issue 4. 2003-07-23. PMID:12513765. |
in order to investigate the role and the mechanism of protein tyrosine phosphatase (ptpase) signaling pathway in the regulation of proliferation, cell cycle and apoptosis in lymphoma cells, the effects of sodium orthovanadate, na(3)vo(4), a specific ptpase inhibitor, were explored on raji lymphoblast-like cell line by mtt assay and cfu-raji culture, morphologic observation, dna gel electrophoresis, fcm and rt-pcr. |
2003-07-23 |
2023-08-12 |
Not clear |
| Ze-Lin Liu, Zuo-Ren Dong, Fu-Xu Wang, Xue-Jun Zhang, Jing-Ci Yang, Wei-Dong Ma, Xing-Yan Du, Li Ya. [Effects of sodium orthovanadate on proliferation and apoptosis in raji cells and its mechanism]. Zhongguo shi yan xue ye xue za zhi. vol 10. issue 4. 2003-07-23. PMID:12513765. |
results showed that mtt assay and cfu-raji culture demonstrated that sodium or thovanadate inhibited the growth of raji cells in a concentration-dependent fashion; morphologic observations showed that raji cells exhibited cytoplasm shrinkage, cytoplasm membrane blebbing, nuclear fragmentation and chromatin condensation forming crescents along nuclear membrane characteristic of apoptosis in the presence of na(3)vo(4); dna gel electrophoresis revealed typical dna ladder reminiscent of dna cleavage at internucleosomal sites in na(3)vo(4) treated cells; fcm and rt-pcr indicated that na(3)vo(4) intervention increased the fraction of annexin v(+) pi(-) cells, reduced the value of mitochondrial transmembrane potential, induced g(2)/m arrest and down-regulated the expression of bcl-2 and cyclin b1 at both mrna and protein level in a concentration-dependent manner. |
2003-07-23 |
2023-08-12 |
Not clear |
| S F Picton, J T McCluskey, P R Flatt, N H McClenagha. Effects of cytotoxic agents on functional integrity and antioxidant enzymes in clonal beta-cells. Diabetes & metabolism. vol 28. issue 6 Pt 2. 2003-07-07. PMID:12688636. |
cell viability was determined using the mtt colorimetric assay and the tunel assay was used to assess apoptosis and acridine orange assay was used to determine levels of apoptosis versus necrosis. |
2003-07-07 |
2023-08-12 |
cat |
| Ahmed M Shoieb, Mona Elgayyar, Paul S Dudrick, John L Bell, Patricia K Titho. In vitro inhibition of growth and induction of apoptosis in cancer cell lines by thymoquinone. International journal of oncology. vol 22. issue 1. 2003-06-16. PMID:12469192. |
tq-induced cytotoxicity was determined using a proliferation assay (mtt assay) and apoptosis assays. |
2003-06-16 |
2023-08-12 |
human |
| Yuhong He, Jing Chen, Jianguo Ren, Guochun Wang, Guoping Ca. Type I collagen inhibits hydroxyl radical-induced apoptosis. Journal of biochemistry. vol 132. issue 3. 2003-05-16. PMID:12204105. |
further study showed that type i collagen also inhibited cell apoptosis induced by.oh, as evidenced by morphological criteria (dapi and annexin v staining) and quantitive assays for apoptotic cells (mtt and flow-cytometric assay for subg1 cells). |
2003-05-16 |
2023-08-12 |
Not clear |
| Jennifer Y Kong, Simon W Rabki. Mitochondrial effects with ceramide-induced cardiac apoptosis are different from those of palmitate. Archives of biochemistry and biophysics. vol 412. issue 2. 2003-05-13. PMID:12667483. |
in embryonic chick cardiomyocytes, ceramide, 100 microm for 24h, induced a 42.9+/-5.8% increase in cell death assessed by the mtt assay, and a significant (p<0.01) 3.9+/-0.6-fold increase in apoptosis assessed by propidium iodide staining of permeabilized cells. |
2003-05-13 |
2023-08-12 |
chicken |
| Jin-Won Hyun, Gi-Jeong Cheon, Hyun-Sook Kim, Yun-Sil Lee, Eun-Young Choi, Byung-Hak Yoon, Jeong-Soon Kim, Myung-Hee Chun. Radiation sensitivity depends on OGG1 activity status in human leukemia cell lines. Free radical biology & medicine. vol 32. issue 3. 2003-05-09. PMID:11827746. |
these were: a dramatic arrest at the g2/m phase indicated by a marked increase in both the number of g2/m cells and the expression of cyclin b1, cdc2, and mitotic phosphoprotein monoclonal-2 (mpm-2)-reactive proteins; a severe apoptosis shown by a marked increase in the number of cells with hypo-diploid dna and dna fragmentation; and as a result, a severe inhibition of cell growth and proliferation measured by the mtt test and [(3)h]-thymidine uptake assay. |
2003-05-09 |
2023-08-12 |
human |