| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Z Gao, H Matsuo, Y Wang, S Nakago, T Maru. Up-regulation by IGF-I of proliferating cell nuclear antigen and Bcl-2 protein expression in human uterine leiomyoma cells. The Journal of clinical endocrinology and metabolism. vol 86. issue 11. 2001-12-13. PMID:11701740. |
the effects of igf-i on bcl-2 protein and pcna expression in cultured leiomyoma cells were assessed by western immunoblot analysis and immunocytochemical staining, whereas the effects of igf-i on the cell viability and apoptosis of the cultured cells were determined by 3-(4,5-dimethylatriazol-2-yl)-2,5diphenyltetrasodium bromide (mtt) assay and terminal deoxynucleotidyl transferase-mediated 2'-deoxyuridine 5'-triphosphate nick end labeling assay, respectively. |
2001-12-13 |
2023-08-12 |
human |
| L Weinrib, J H Li, J Donovan, D Huang, F F Li. Cisplatin chemotherapy plus adenoviral p53 gene therapy in EBV-positive and -negative nasopharyngeal carcinoma. Cancer gene therapy. vol 8. issue 5. 2001-09-27. PMID:11477455. |
clonogenic and mtt assays were used to assess the sensitivity of cells to these treatments, and apoptosis was also quantified. |
2001-09-27 |
2023-08-12 |
human |
| Y Muneta, Y Shimoji, S Inumaru, Y Mor. Molecular cloning, characterization, and expression of porcine Fas ligand (CD95 ligand). Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research. vol 21. issue 5. 2001-08-23. PMID:11429161. |
the recombinant porcine fasl expressed by recombinant baculovirus containing the whole coding sequences of porcine fasl showed cytotoxic effect and induced apoptosis in porcine renal tubular cell line pk-15 cells sensitized by cycloheximide (chx), which was confirmed by mtt assay, dna fragmentation assay, and tunel staining, respectively. |
2001-08-23 |
2023-08-12 |
human |
| E Lee, S H Jeon, J Y Yi, Y J Jin, Y S So. Calcipotriol inhibits autocrine phosphorylation of EGF receptor in a calcium-dependent manner, a possible mechanism for its inhibition of cell proliferation and stimulation of cell differentiation. Biochemical and biophysical research communications. vol 284. issue 2. 2001-07-12. PMID:11394895. |
based on mtt assay, pcna staining, dapi staining, and involucrin immunocytochemical staining, we showed that calcipotriol inhibited cell growth and stimulated differentiation but did not induce apoptosis. |
2001-07-12 |
2023-08-12 |
Not clear |
| B Montaner, R Pérez-Tomá. Prodigiosin-induced apoptosis in human colon cancer cells. Life sciences. vol 68. issue 17. 2001-06-14. PMID:11388704. |
dld-1 and sw-620 human colon adenocarcinoma cells, nrk and swiss-3t3 nonmalignant cells were assayed by the mtt assay, fragmentation pattern of dna, hoechst 33342 staining and study of parp cleavage by western blot, in order to characterize the prodigiosin-induced apoptosis. |
2001-06-14 |
2023-08-12 |
human |
| S Yokoyama, H Hirano, N Wakimaru, K P Sarker, J Kurats. Inhibitory effect of epigallocatechin-gallate on brain tumor cell lines in vitro. Neuro-oncology. vol 3. issue 1. 2001-05-31. PMID:11305413. |
cell viability was determined by assay with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (mtt), and the extent of apoptosis was studied by flow cytometric analysis. |
2001-05-31 |
2023-08-12 |
human |
| K Sennvik, E Benedikz, J Fastbom, E Sundström, B Winblad, M Ankarcron. Calcium ionophore A23187 specifically decreases the secretion of beta-secretase cleaved amyloid precursor protein during apoptosis in primary rat cortical cultures. Journal of neuroscience research. vol 63. issue 5. 2001-05-17. PMID:11223918. |
cell viability was determined by mtt measurements and apoptosis was further confirmed by annexin v and propidium iodide staining. |
2001-05-17 |
2023-08-12 |
rat |
| J G Zhang, S Ghosh, C D Ockleford, M Galiñane. Characterization of an in vitro model for the study of the short and prolonged effects of myocardial ischaemia and reperfusion in man. Clinical science (London, England : 1979). vol 99. issue 5. 2001-03-15. PMID:11052925. |
however, the results of mtt reduction show that there is a progressive decrease in tissue viability over the 24 h reperfusion period, possibly reflecting the occurrence of tissue necrosis and apoptosis at different reperfusion times. |
2001-03-15 |
2023-08-12 |
human |
| S V Yim, K H Kim, C J Kim, J H Chun. Serotonin induces apoptosis in PGT-beta pineal gland tumor cells. Japanese journal of pharmacology. vol 84. issue 1. 2001-02-15. PMID:11043456. |
to investigate the involvement of serotonin-induced apoptosis as a potential mechanism in the pathophysiology of serotonin-related diseases, mtt (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay, dapi (4',6-diamidino-2-phenylindole) staining, tunel (terminal deoxynucleotidyl transferase-mediated dutp-biotin nick end labeling) assay and flow cytometric analysis were performed using the immortalized pineal cell line pgt-beta. |
2001-02-15 |
2023-08-12 |
Not clear |
| C M Perks, C McCaig, J M Holl. Differential insulin-like growth factor (IGF)-independent interactions of IGF binding protein-3 and IGF binding protein-5 on apoptosis in human breast cancer cells. Involvement of the mitochondria. Journal of cellular biochemistry. vol 80. issue 2. 2001-01-26. PMID:11074596. |
1: they were incubated with increasing doses of antimycin a for 24 h. 2: they were coincubated with an apoptotic dose of either c2 or rgd together with a nonapoptotic dose of antimycin a for 24 h. 3: they were incubated with a binding protein (100 ng/ml) for 24 h followed by coincubation of the binding protein with an apoptotic dose of antimycin a for a further 24 h. cell viability was assessed by trypan blue dye exclusion and mtt assay, and apoptosis was confirmed and measured by morphologic assessment and flow cytometry. |
2001-01-26 |
2023-08-12 |
human |
| D Lamarque, A P Moran, Z Szepes, J C Delchier, B J Whittl. Cytotoxicity associated with induction of nitric oxide synthase in rat duodenal epithelial cells in vivo by lipopolysaccharide of Helicobacter pylori: inhibition by superoxide dismutase. British journal of pharmacology. vol 130. issue 7. 2001-01-25. PMID:10928954. |
the epithelial cell viability, as assessed by trypan blue exclusion and mtt conversion, was reduced 5 h after challenge with h. pylori lps, while the incidence of apoptosis was increased. |
2001-01-25 |
2023-08-12 |
rat |
| C A Fowler, C M Perks, P V Newcomb, P B Savage, J R Farndon, J M Holl. Insulin-like growth factor binding protein-3 (IGFBP-3) potentiates paclitaxel-induced apoptosis in human breast cancer cells. International journal of cancer. vol 88. issue 3. 2000-11-13. PMID:11054675. |
paclitaxel-induced apoptosis was assessed in human hs578t breast cancer cells, using the mtt assay, cell counting, morphological features and flow cytometry. |
2000-11-13 |
2023-08-12 |
human |
| L Maksumova, K Ohnishi, F Muratkhodjaev, W Zhang, L Pan, A Takeshita, R Ohn. Increased sensitivity of multidrug-resistant myeloid leukemia cell lines to lovastatin. Leukemia. vol 14. issue 8. 2000-08-24. PMID:10942241. |
mtt and apoptosis assays revealed that k562/adr, nomo-1/adr and nb4/ra/mdr were more sensitive to lovastatin than their parental cell lines, while nb4/mdr showed the same level of sensitivity as parental nb4 cells, which already were very sensitive to lovastatin. |
2000-08-24 |
2023-08-12 |
Not clear |
| J V Vadgama, Y Wu, D Shen, S Hsia, J Bloc. Effect of selenium in combination with Adriamycin or Taxol on several different cancer cells. Anticancer research. vol 20. issue 3A. 2000-08-17. PMID:10928049. |
the effect of selenium was assessed by apoptosis; dna synthesis; growth rate by mtt assay; uptake of amino acid meaib by system a; and morphological changes. |
2000-08-17 |
2023-08-12 |
Not clear |
| X W Zhang, C Qing, B X. Apoptosis induction and cell cycle perturbation in human hepatoma hep G2 cells by 10-hydroxycamptothecin. Anti-cancer drugs. vol 10. issue 6. 2000-07-21. PMID:10885905. |
we studied hcpt to determine whether or not its anti-hepatoma activity occurs through apoptosis induction and cell cycle disturbance using the mtt method, dapi staining, agarose gel electrophoresis and flow cytometric analysis. |
2000-07-21 |
2023-08-12 |
human |
| D Tong, M Poot, D Hu, D Od. 5-Fluorouracil-induced apoptosis in cultured oral cancer cells. Oral oncology. vol 36. issue 2. 2000-07-17. PMID:10745178. |
we investigated the pathway of apoptosis using the pan-caspase inhibitor idn-1529 by methylthiazolyl diphenyl tetrazolium bromide (mtt) colorimetric analysis. |
2000-07-17 |
2023-08-12 |
human |
| S Kanno, M Ishikawsa, M Takayanagi, Y Takayanagi, K Sasak. Potentiation of acetaminophen hepatotoxicity by doxapram in mouse primary cultured hepatocytes. Biological & pharmaceutical bulletin. vol 23. issue 4. 2000-06-21. PMID:10784425. |
loss of viability on exposure to aa and/or dop in cultured hepatocytes was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (mtt) assay and the apoptosis of cultured hepatocytes was detected by nuclear morphologic observation and from a ladder-like dna fragmentation pattern. |
2000-06-21 |
2023-08-12 |
mouse |
| D Lobne. Comparison of the LDH and MTT assays for quantifying cell death: validity for neuronal apoptosis? Journal of neuroscience methods. vol 96. issue 2. 2000-05-08. PMID:10720679. |
comparison of the ldh and mtt assays for quantifying cell death: validity for neuronal apoptosis? |
2000-05-08 |
2023-08-12 |
Not clear |
| D Lobne. Comparison of the LDH and MTT assays for quantifying cell death: validity for neuronal apoptosis? Journal of neuroscience methods. vol 96. issue 2. 2000-05-08. PMID:10720679. |
neuronal apoptosis induced in cortical cultures by exposure to serum deprivation, staurosporine, nifedipine, or c2-ceramide was assayed by lactate dehydrogenase (ldh) release or inhibition of 3-(4, 5-dimethylthiazol-2-yl)2,5-diphenyl-tetrazolium bromide (mtt) reduction. |
2000-05-08 |
2023-08-12 |
Not clear |
| U Ozgen, S Savaşan, M Stout, S Buck, Y Ravindranat. Further elucidation of mechanism of resistance to vincristine in myeloid cells: role of hypochlorous acid in degradation of vincristine by myeloperoxidase. Leukemia. vol 14. issue 1. 2000-02-02. PMID:10637476. |
vcr cytotoxicity was evaluated by mtt assay and by quantitative measurement of apoptosis. |
2000-02-02 |
2023-08-12 |
human |