| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Jiangqi Xu, Xiaohong Huang, Shixiang Liu, Dongdong Chen, Yufang Xie, Zhenwu Zha. The protective effects of lncRNA ZFAS1/miR-421-3p/MEF2C axis on cerebral ischemia-reperfusion injury. Cell cycle (Georgetown, Tex.). 2022-07-26. PMID:35880950. |
overexpressed or silenced plasmids of zfas1 were transfected into the cells to analyze the effects of oxygen-glucose deprivation/reperfusion (ogd/r) treatment on the viability, apoptosis and related gene expressions of neuro-2a cell by performing mtt assay, flow cytometry, qrt-pcr, and western blot. |
2022-07-26 |
2023-08-14 |
mouse |
| Bo Min, Yan Wang, Feng Liang, Cheng-Xiang Wang, Fan Wang, Zhi Yan. The Protective Mechanism of Afuresertib against Esophageal Cancer. Disease markers. vol 2022. 2022-07-25. PMID:35872696. |
in this study, we determined the effect of different concentrations of afuresertib on cell viability by mtt assay and determined the effect of afuresertib on cell apoptosis by annexin v-fitc/pi dual staining. |
2022-07-25 |
2023-08-14 |
rat |
| Marianna Oláh, Enikő Farkas, Inna Székács, Robert Horvath, András Székác. Cytotoxic effects of Roundup Classic and its components on NE-4C and MC3T3-E1 cell lines determined by biochemical and flow cytometric assays. Toxicology reports. vol 9. 2022-07-25. PMID:35875257. |
higher sensitivity differences were detected in the viability test by flow cytometry (7-9-fold), than by the mtt assay (1.5-3-fold); in the genotoxicity test by the comet assay (3.5-403-fold), than by the dna-damage test (9.3-158-fold); and in the apoptosis test by the annexin v dead cell kit (1.1-12.7-fold), than by the caspase 3/7 kit (1-6.5-fold). |
2022-07-25 |
2023-08-14 |
Not clear |
| Mostafa Ghorban Khan Tafreshi, Zohreh Mazaheri, Mansour Heidari, Nahid Babaei, Abbas Doost. Induction of Apoptosis in the U937 Cell Line Co-cultured with Adipose-derived Stem Cells Secreting Bone Morphogenetic Protein-4. International journal of molecular and cellular medicine. vol 10. issue 4. 2022-07-25. PMID:35875334. |
the induction of cell death and apoptosis in the u937 cell line was assessed using mtt and annexin v/ pi assays, respectively. |
2022-07-25 |
2023-08-14 |
human |
| Yei-Jin Kang, Won-Geun Yang, Weon-Sik Chae, Dae-Won Kim, Seong-Gon Kim, Horatiu Rotar. Administration of 4‑hexylresorcinol increases p53‑mediated transcriptional activity in oral cancer cells with the p53 mutation. Oncology reports. vol 48. issue 3. 2022-07-20. PMID:35856441. |
to determine the effect of 4hr on p53 mutant carcinoma cells, western blot analysis, p53 transcriptional activity analysis, mtt assay and apoptosis immunocytochemistry were performed. |
2022-07-20 |
2023-08-14 |
human |
| Zimin Li, Junzheng Zheng, Jian Xu, Quan Ni. Knockdown of Circ_0000798 Impedes Cell Growth and Motility of Renal Cell Carcinoma Cells Through Functioning as miRNA Sponge for miR-589-5p. Biochemical genetics. 2022-07-20. PMID:35857217. |
in vitro assays, including mtt, edu, transwell, and plate colony, scratch wound, apoptosis, and cell cycle assays, and in vivo xenograft tumor model were launched to measure cell dysfunctions. |
2022-07-20 |
2023-08-14 |
Not clear |
| Mei-Zhu Wu, Yun Xiang, Cui-Yu Bao, Tao Li. [Protective effect of salidroside on high fat-induced apoptosis in H9c2 cardiomyocytes through AMPK/mTOR/p70S6K pathway]. Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica. vol 47. issue 14. 2022-07-19. PMID:35850842. |
the study explored the effect of salidroside(sal) on high fat-induced apoptosis in h9 c2 cardiomyocytes based on ampk/mtor/p70 s6 k pathway.h9 c2 cardiomyocytes were cultured in vitro and the lipotoxicity model of h9 c2 cardiomyocytes was constructed by 0.2 mmol·l~(-1) palmitic acid(pa) treatment for 24 hours.the cells were divided into control group, pa group, and sal group(20 μmol·l~(-1)).cell proliferation was detected with cell proliferation kit i(mtt) assay after sal and pa treatment.dihydroethidium(dhe) probe, annexin v-fitc/pi kit, and jc-1 probe were used to estimate reactive oxygen species(ros) level, cell apoptosis, and mitochondrial membrane potential(mmp) change, respectively.the expression levels of p-ampk/ampk, p-mtor/mtor, p-p70 s6 k/p70 s6 k and apoptosis-related proteins bax, bcl-2, and cleaved caspase-3 were investigated with western blot.the mrna levels of ampk, mtor and p70 s6 k were determined by quantitative reverse transcription-polymerase chain reaction(qrt-pcr).results:: showed that compared with control group, pa group had decreased cell proliferation ability, mmp, bcl-2 protein expression and ampk protein and mrna expression, while increased ros level, bax and cleaved caspase-3 protein expression, and mtor and p70 s6 k mrna and protein expression, and the difference was statistically significant(p<0.05, p<0.01).compared with pa group, sal improved cell proliferation ability, mmp level, bcl-2 protein expression, and ampk mrna and protein expression, while down-regulated ros level, cell apoptosis, bax and cleaved caspase-3 protein expression, and mtor and p70 s6 k mrna and protein expression, and the difference was statistically significant(p<0.05, p<0.01).in conclusion, sal exerted protective effects on high fat-induced lipotoxicity of h9 c2 cardiomyocytes, alleviated the oxidative stress injury and reduced cell apoptosis via regulating ampk/mtor/p70 s6 k signaling pathway. |
2022-07-19 |
2023-08-14 |
Not clear |
| Lin Zhu, Sha Liu, Yi-Fan Liao, Yan-Mei Sheng, Ji-Chun He, Zheng-Xue Cai, Qiong Man, Yi-Ying W. Calycosin suppresses colorectal cancer progression by targeting ERβ, upregulating PTEN, and inhibiting PI3K/Akt signal pathway. Cell biology international. 2022-07-17. PMID:35842774. |
in this study, we analyzed the effect of calycosin on the viability and apoptosis of human crc hct116 and sw480 cells via mtt assay, flow cytometry assay, and caspase-3/7 activity assay. |
2022-07-17 |
2023-08-14 |
mouse |
| Yuting Xu, Cheng Qian, Chunxiao Liu, Yingqiang Fu, Kaiyuan Zhu, Zhenbo Niu, Jiaxin Li. Investigation of the Mechanism of hsa_circ_000 1429 Adsorbed miR-205 to Regulate KDM4A and Promote Breast Cancer Metastasis. Contrast media & molecular imaging. vol 2022. 2022-07-14. PMID:35833065. |
rip verifies that hsa_circ_000 1429 binds to ago2; rna pull down results prove that hsa_circ_000 1429 binds to mir-205; mtt detects cell proliferation; transwell assay detects cell migration and invasion ability; flow cytometry detects cell apoptosis rate. |
2022-07-14 |
2023-08-14 |
human |
| Yu Jin Jung, Hyunsu Choi, Eungseok O. Melatonin attenuates MPP Biochemical and biophysical research communications. vol 621. 2022-07-10. PMID:35810592. |
cell viability and apoptosis were measured using mtt and dapi. |
2022-07-10 |
2023-08-14 |
Not clear |
| Farkas Sarnyai, Éva Kereszturi, Kitti Szirmai, Judit Mátyási, Johanna Iman Al-Hag, Tamás Csizmadia, Péter Lőw, Péter Szelényi, Viola Tamási, Kinga Tibori, Veronika Zámbó, Blanka Tóth, Miklós Csal. Different Metabolism and Toxicity of TRANS Fatty Acids, Elaidate and Vaccenate Compared to Cis-Oleate in HepG2 Cells. International journal of molecular sciences. vol 23. issue 13. 2022-07-09. PMID:35806300. |
cellular fas, triacylglycerols, diacylglycerols and ceramides were quantitated using chromatography, markers of stress and apoptosis were assessed at mrna and protein levels, ultrastructural changes were examined by electron microscopy and viability was evaluated by mtt assay. |
2022-07-09 |
2023-08-14 |
human |
| Zhang Yang, Wang Yaling, Liu Tao, Rao Mingyue, Li Yongju. Cardioprotective effect of NRG-4 gene expression on spontaneous hypertension rats and its mechanism through mediating the activation of ErbB signaling pathway. Cellular and molecular biology (Noisy-le-Grand, France). vol 68. issue 1. 2022-07-09. PMID:35809324. |
quantitative real-time polymerase chain reaction (qrt-pcr) and western blot were used to detect the expression levels in addition, methyl thiazolyl tetrazolium salt (mtt) assay and flow cytometry were performed to detect the proliferation and apoptosis of cardiomyocytes, respectively. |
2022-07-09 |
2023-08-14 |
rat |
| Ziqi Meng, Rui Zhang, Xuwei Wu, Tiefeng Jin, Meihua Zhan. [Ginsenoside Rg3 Regulates Cisplatin Resistance in Gastric Cancer by Wnt/β-catenin Signaling Pathway]. Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae. vol 44. issue 3. 2022-07-06. PMID:35791931. |
objective to investigate the inhibitory effect of ginsenoside rg3 combined with cisplatin (ddp) on ddp-resistant cell line sgc-7901/ddp and their molecular mechanism.methods sgc-7901/ddp cells were divided into four groups including a control group,a ginsenoside rg3 (40 μg/ml) treatment group,a ddp (1.40 μg/ml) treatment group,and a drug combination treatment group.the proliferation ability of sgc-7901/ddp cells was detected by mtt,edu,and colony formation assays.the apoptosis ability of sgc-7901/ddp cell was detected by flow cytometry and hoechst 33342 staining.the protein levels of apoptosis-related markers were detected by western blotting.the migration ability of sgc-7901/ddp cells was detected by wound healing and transwell assays.the expression levels of proteins in epithelial-mesenchymal transformation (emt) and wnt/β-catenin signaling pathway were determined by western blotting and immunofluorescence staining.results compared with the ginsenoside rg3 or the ddp treatment groups,the drug combination treatment group inhibited the proliferation ( |
2022-07-06 |
2023-08-14 |
Not clear |
| Zhiqin Qiu, Lin He, Feng Yu, Hui Lv, Ye Zho. LncRNA FAM13A-AS1 Regulates Proliferation and Apoptosis of Cervical Cancer Cells by Targeting miRNA-205-3p/DDI2 Axis. Journal of oncology. vol 2022. 2022-07-05. PMID:35783157. |
mtt, flow cytometry, and transwell assays were performed to explore the influence of fam13a-as1 on cervical cancer cell proliferation, apoptosis, invasion, and migration. |
2022-07-05 |
2023-08-14 |
mouse |
| Ruifang Sun, Zhigang Liu, Yun Lv, Yanqi Yang, Yang Yang, Yu Xiang, Qiuyu Jiang, Chang'an Zhao, Moqi Lv, Jian Zhang, Juan Zhang, Caixia Ding, Dangxia Zho. FOCAD/miR-491-5p, downregulated by EGR1, function as tumor suppressor by inhibiting the proliferation and migration of gastric cancer cells. Progress in biophysics and molecular biology. 2022-07-05. PMID:35788362. |
mtt, colony, transwell chamber, and flow cytometry assays revealed that sifocad promoted cell proliferation, growth, and migration, and inhibited apoptosis. |
2022-07-05 |
2023-08-14 |
human |
| Nana Su, Lanlan Li, Erle Zhou, Hong Li, Shuhua Wu, Zhang Ca. Resveratrol Downregulates miR-155-5p to Block the Malignant Behavior of Gastric Cancer Cells. BioMed research international. vol 2022. 2022-07-05. PMID:35789645. |
the results of mtt assay, plate clone formation test, cell scratch test, transwell test, and flow cytometry showed that mir-155-5p promoted the proliferation, invasion, and metastasis of gastric cancer cell lines and inhibited apoptosis, while res addition inhibited this effect ( |
2022-07-05 |
2023-08-14 |
Not clear |
| Tanachapa Jantalika, Sirikul Manochantr, Pakpoom Kheolamai, Duangrat Tantikanlayaporn, Weerachai Saijuntha, Somchai Pinlaor, Arthit Chairoungdua, Luminita Paraoan, Chairat Tantrawatpa. Human chorion-derived mesenchymal stem cells suppress JAK2/STAT3 signaling and induce apoptosis of cholangiocarcinoma cell lines. Scientific reports. vol 12. issue 1. 2022-07-05. PMID:35790790. |
mtt assay, annexin v/pi analysis, and jc-1 staining were used to assess the effects of ch-cm on proliferation and apoptosis of cca cells, respectively. |
2022-07-05 |
2023-08-14 |
human |
| Yueh-Min Lin, Jiro Hasegawa Situmorang, Jia-Zun Guan, Dennis Jine-Yuan Hsieh, Jaw-Ji Yang, Michael Yu-Chih Chen, Ching-Hui Loh, Chia-Hua Kuo, Shang-Yeh Lu, Ying-Ming Liou, Chih-Yang Huan. ZAKβ Alleviates Oxidized Low-density Lipoprotein (ox-LDL)-Induced Apoptosis and B-type Natriuretic Peptide (BNP) Upregulation in Cardiomyoblast. Cell biochemistry and biophysics. 2022-07-01. PMID:35776316. |
cell viability and apoptosis were measured by mtt and tunel assay, respectively. |
2022-07-01 |
2023-08-14 |
Not clear |
| Hongjie Chen, Ziyi Wang, Ninggang Yang, Jun Zhang, Zhong Lian. Decorin inhibits proliferation and metastasis in human bladder cancer cells by upregulating P21. Medicine. vol 101. issue 26. 2022-07-01. PMID:35777025. |
mtt, flow cytometry, and wound/transwell assays were used to detect cell proliferation, cycle arrest, apoptosis, migration, and invasion. |
2022-07-01 |
2023-08-14 |
human |
| Qi Liu, Feng Li. Lentivirus-induced knockdown of IARS2 expression inhibits the proliferation and promotes the apoptosis of human osteosarcoma cells. Oncology letters. vol 24. issue 2. 2022-06-29. PMID:35765273. |
the effects of iars2 knockdown on cell proliferation, colony formation and apoptosis were evaluated by celigo, mtt assays, colony formation assays and flow cytomeric analysis. |
2022-06-29 |
2023-08-14 |
human |