| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Lin Zhu, Sha Liu, Yi-Fan Liao, Yan-Mei Sheng, Ji-Chun He, Zheng-Xue Cai, Qiong Man, Yi-Ying W. Calycosin suppresses colorectal cancer progression by targeting ERβ, upregulating PTEN, and inhibiting PI3K/Akt signal pathway. Cell biology international. 2022-07-17. PMID:35842774. |
in this study, we analyzed the effect of calycosin on the viability and apoptosis of human crc hct116 and sw480 cells via mtt assay, flow cytometry assay, and caspase-3/7 activity assay. |
2022-07-17 |
2023-08-14 |
mouse |
| Yuting Xu, Cheng Qian, Chunxiao Liu, Yingqiang Fu, Kaiyuan Zhu, Zhenbo Niu, Jiaxin Li. Investigation of the Mechanism of hsa_circ_000 1429 Adsorbed miR-205 to Regulate KDM4A and Promote Breast Cancer Metastasis. Contrast media & molecular imaging. vol 2022. 2022-07-14. PMID:35833065. |
rip verifies that hsa_circ_000 1429 binds to ago2; rna pull down results prove that hsa_circ_000 1429 binds to mir-205; mtt detects cell proliferation; transwell assay detects cell migration and invasion ability; flow cytometry detects cell apoptosis rate. |
2022-07-14 |
2023-08-14 |
human |
| Yu Jin Jung, Hyunsu Choi, Eungseok O. Melatonin attenuates MPP Biochemical and biophysical research communications. vol 621. 2022-07-10. PMID:35810592. |
cell viability and apoptosis were measured using mtt and dapi. |
2022-07-10 |
2023-08-14 |
Not clear |
| Farkas Sarnyai, Éva Kereszturi, Kitti Szirmai, Judit Mátyási, Johanna Iman Al-Hag, Tamás Csizmadia, Péter Lőw, Péter Szelényi, Viola Tamási, Kinga Tibori, Veronika Zámbó, Blanka Tóth, Miklós Csal. Different Metabolism and Toxicity of TRANS Fatty Acids, Elaidate and Vaccenate Compared to Cis-Oleate in HepG2 Cells. International journal of molecular sciences. vol 23. issue 13. 2022-07-09. PMID:35806300. |
cellular fas, triacylglycerols, diacylglycerols and ceramides were quantitated using chromatography, markers of stress and apoptosis were assessed at mrna and protein levels, ultrastructural changes were examined by electron microscopy and viability was evaluated by mtt assay. |
2022-07-09 |
2023-08-14 |
human |
| Zhang Yang, Wang Yaling, Liu Tao, Rao Mingyue, Li Yongju. Cardioprotective effect of NRG-4 gene expression on spontaneous hypertension rats and its mechanism through mediating the activation of ErbB signaling pathway. Cellular and molecular biology (Noisy-le-Grand, France). vol 68. issue 1. 2022-07-09. PMID:35809324. |
quantitative real-time polymerase chain reaction (qrt-pcr) and western blot were used to detect the expression levels in addition, methyl thiazolyl tetrazolium salt (mtt) assay and flow cytometry were performed to detect the proliferation and apoptosis of cardiomyocytes, respectively. |
2022-07-09 |
2023-08-14 |
rat |
| Ziqi Meng, Rui Zhang, Xuwei Wu, Tiefeng Jin, Meihua Zhan. [Ginsenoside Rg3 Regulates Cisplatin Resistance in Gastric Cancer by Wnt/β-catenin Signaling Pathway]. Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae. vol 44. issue 3. 2022-07-06. PMID:35791931. |
objective to investigate the inhibitory effect of ginsenoside rg3 combined with cisplatin (ddp) on ddp-resistant cell line sgc-7901/ddp and their molecular mechanism.methods sgc-7901/ddp cells were divided into four groups including a control group,a ginsenoside rg3 (40 μg/ml) treatment group,a ddp (1.40 μg/ml) treatment group,and a drug combination treatment group.the proliferation ability of sgc-7901/ddp cells was detected by mtt,edu,and colony formation assays.the apoptosis ability of sgc-7901/ddp cell was detected by flow cytometry and hoechst 33342 staining.the protein levels of apoptosis-related markers were detected by western blotting.the migration ability of sgc-7901/ddp cells was detected by wound healing and transwell assays.the expression levels of proteins in epithelial-mesenchymal transformation (emt) and wnt/β-catenin signaling pathway were determined by western blotting and immunofluorescence staining.results compared with the ginsenoside rg3 or the ddp treatment groups,the drug combination treatment group inhibited the proliferation ( |
2022-07-06 |
2023-08-14 |
Not clear |
| Zhiqin Qiu, Lin He, Feng Yu, Hui Lv, Ye Zho. LncRNA FAM13A-AS1 Regulates Proliferation and Apoptosis of Cervical Cancer Cells by Targeting miRNA-205-3p/DDI2 Axis. Journal of oncology. vol 2022. 2022-07-05. PMID:35783157. |
mtt, flow cytometry, and transwell assays were performed to explore the influence of fam13a-as1 on cervical cancer cell proliferation, apoptosis, invasion, and migration. |
2022-07-05 |
2023-08-14 |
mouse |
| Ruifang Sun, Zhigang Liu, Yun Lv, Yanqi Yang, Yang Yang, Yu Xiang, Qiuyu Jiang, Chang'an Zhao, Moqi Lv, Jian Zhang, Juan Zhang, Caixia Ding, Dangxia Zho. FOCAD/miR-491-5p, downregulated by EGR1, function as tumor suppressor by inhibiting the proliferation and migration of gastric cancer cells. Progress in biophysics and molecular biology. 2022-07-05. PMID:35788362. |
mtt, colony, transwell chamber, and flow cytometry assays revealed that sifocad promoted cell proliferation, growth, and migration, and inhibited apoptosis. |
2022-07-05 |
2023-08-14 |
human |
| Nana Su, Lanlan Li, Erle Zhou, Hong Li, Shuhua Wu, Zhang Ca. Resveratrol Downregulates miR-155-5p to Block the Malignant Behavior of Gastric Cancer Cells. BioMed research international. vol 2022. 2022-07-05. PMID:35789645. |
the results of mtt assay, plate clone formation test, cell scratch test, transwell test, and flow cytometry showed that mir-155-5p promoted the proliferation, invasion, and metastasis of gastric cancer cell lines and inhibited apoptosis, while res addition inhibited this effect ( |
2022-07-05 |
2023-08-14 |
Not clear |
| Tanachapa Jantalika, Sirikul Manochantr, Pakpoom Kheolamai, Duangrat Tantikanlayaporn, Weerachai Saijuntha, Somchai Pinlaor, Arthit Chairoungdua, Luminita Paraoan, Chairat Tantrawatpa. Human chorion-derived mesenchymal stem cells suppress JAK2/STAT3 signaling and induce apoptosis of cholangiocarcinoma cell lines. Scientific reports. vol 12. issue 1. 2022-07-05. PMID:35790790. |
mtt assay, annexin v/pi analysis, and jc-1 staining were used to assess the effects of ch-cm on proliferation and apoptosis of cca cells, respectively. |
2022-07-05 |
2023-08-14 |
human |
| Yueh-Min Lin, Jiro Hasegawa Situmorang, Jia-Zun Guan, Dennis Jine-Yuan Hsieh, Jaw-Ji Yang, Michael Yu-Chih Chen, Ching-Hui Loh, Chia-Hua Kuo, Shang-Yeh Lu, Ying-Ming Liou, Chih-Yang Huan. ZAKβ Alleviates Oxidized Low-density Lipoprotein (ox-LDL)-Induced Apoptosis and B-type Natriuretic Peptide (BNP) Upregulation in Cardiomyoblast. Cell biochemistry and biophysics. 2022-07-01. PMID:35776316. |
cell viability and apoptosis were measured by mtt and tunel assay, respectively. |
2022-07-01 |
2023-08-14 |
Not clear |
| Hongjie Chen, Ziyi Wang, Ninggang Yang, Jun Zhang, Zhong Lian. Decorin inhibits proliferation and metastasis in human bladder cancer cells by upregulating P21. Medicine. vol 101. issue 26. 2022-07-01. PMID:35777025. |
mtt, flow cytometry, and wound/transwell assays were used to detect cell proliferation, cycle arrest, apoptosis, migration, and invasion. |
2022-07-01 |
2023-08-14 |
human |
| Qi Liu, Feng Li. Lentivirus-induced knockdown of IARS2 expression inhibits the proliferation and promotes the apoptosis of human osteosarcoma cells. Oncology letters. vol 24. issue 2. 2022-06-29. PMID:35765273. |
the effects of iars2 knockdown on cell proliferation, colony formation and apoptosis were evaluated by celigo, mtt assays, colony formation assays and flow cytomeric analysis. |
2022-06-29 |
2023-08-14 |
human |
| Zengmian Wang, Zhimin Na, Ying Cui, Chunjie Wei, Shuqiu Wan. LncRNA ZFAS1 regulates the hippocampal neurons injury in epilepsy through the miR-15a-5p/OXSR1/NF-κB pathway. Metabolic brain disease. 2022-06-25. PMID:35751788. |
cell viability and apoptosis were examined by mtt assay, edu staining and flow cytometry. |
2022-06-25 |
2023-08-14 |
mouse |
| Tian Xie, Jun Yuan, Ling Mei, Ping Li, Ruijie Pa. Luteolin suppresses TNF-α-induced inflammatory injury and senescence of nucleus pulposus cells via the Sirt6/NF-κB pathway. Experimental and therapeutic medicine. vol 24. issue 1. 2022-06-24. PMID:35747154. |
cell viability and apoptosis were assessed by mtt assay and tunel staining, respectively. |
2022-06-24 |
2023-08-14 |
human |
| Zundong Wang, Zhen Zeng, Yongsheng Hu, Hengcan Sun, Ying Tang, Weiqin Li. Network Pharmacology and Pharmacological Mechanism of CV-3 in Atrial Fibrillation. Evidence-based complementary and alternative medicine : eCAM. vol 2022. 2022-06-24. PMID:35747377. |
flow cytometry and the mtt assay were employed to detect cell apoptosis and cell viability, respectively. |
2022-06-24 |
2023-08-14 |
Not clear |
| Xin Zhang, Xuebing Zhou, Ming Gao, You Lyu, Ying Wang, Chunyu Yang, Yingshi Piao, Xiangshan Re. [Cordycepin inhibits the proliferation and migration of human gastric cancer cells by suppressing lipid metabolism via AMPK and MAPK activation]. Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology. vol 38. issue 6. 2022-06-22. PMID:35732609. |
the proliferation ability of mgc-803 and hgc-27 cells was detected by mtt assay and edu assay; the colony formation ability of cells was detected by colony formation assay; both wound healing assay and cell migration assay were applied to detect the cell migration ability of mgc-803 and hgc-27 cells; the chromatin agglutination was detected by hoechst 33342 staining; the apoptosis of gastric cancer cells was detected by annexin v-fitc/pi double labeling combined with flow cytometry; western blot was used to measure the protein expression levels of lipid metabolism-related proteins including sterol regulatory element binding transcription factor 1 (srebf1), fatty acid synthase (fasn), and acetyl coa carboxylase 1 (acc1), epithelial-mesenchymal transition (emt)-related proteins including e-cadherin, vimentin, snail, slug, matrix metalloproteinase 2 (mmp2), mmp9, ampk, and phosphorylated ampk (p-ampk), mapk signaling pathway-related proteins including jnk, phosphorylated jnk (p-jnk), p38 mapk, and p-p38 mapk, and apoptosis-related proteins including cleaved caspase-9 (c-caspase-9), c-caspase-3, and cleaved poly (adp-ribose) polymerase (c-parp). |
2022-06-22 |
2023-08-14 |
human |
| Hulya Elbe, Feral Ozturk, Gurkan Yigitturk, Tuba Baygar, Turker Cavusogl. Anticancer activity of linalool: comparative investigation of ultrastructural changes and apoptosis in breast cancer cells. Ultrastructural pathology. 2022-06-21. PMID:35727696. |
mtt assay for cell proliferation and annexin v assay for apoptosis was done. |
2022-06-21 |
2023-08-14 |
human |
| Yasin Tülüce, Azhee Ibrahim Hussein, İsmail Koyuncu, Ahmet Kiliç, Mustafa Durgu. The effect of a bis-structured Schiff base on apoptosis, cytotoxicity, and DNA damage of breast cancer cells. Journal of biochemical and molecular toxicology. 2022-06-20. PMID:35719061. |
in this perspective, the mcf-7 cell line was cultured for the examination of different molecular techniques, including mtt, apoptosis analysis by enzyme-linked immunosorbent assay (elisa), and comet assay. |
2022-06-20 |
2023-08-14 |
human |
| Coral Estefania Gutiérrez-Almeida, Anne Santerre, Lilia Carolina León-Moreno, Irene Guadalupe Aguilar-García, Rolando Castañeda-Arellano, Sergio Horacio Dueñas-Jiménez, Judith Marcela Dueñas-Jiméne. Proliferation and apoptosis regulation by G protein-coupled estrogen receptor in glioblastoma C6 cells. Oncology letters. vol 24. issue 1. 2022-06-20. PMID:35720489. |
cell viability was examined using the mtt assay and apoptosis using caspase-3 immunostaining and elisa. |
2022-06-20 |
2023-08-14 |
human |