| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Xinyue He, Sanying Wang, Bing Liu, Di Jiang, Fen Chen, Genxiang Mao, Weihua Jin, Hongying Pan, Weihong Zhon. Sulfated modification of hyaluronan tetrasaccharide enhances its antitumor activity on human lung adenocarcinoma A549 cells in vitro and in vivo. Bioorganic & medicinal chemistry letters. 2022-08-20. PMID:35987509. |
then, the anti-tumor activity was detected via mtt assay and xenograft tumor experiments, while the expression level change of apoptosis genes was analyzed by qrt-pcr. |
2022-08-20 |
2023-08-14 |
human |
| Yuan Wei, Liang Xiao, Liu Yingying, Wang Haiche. Pinoresinol diglucoside ameliorates H/R-induced injury of cardiomyocytes by regulating miR-142-3p and HIF1AN. Journal of biochemical and molecular toxicology. 2022-08-13. PMID:35962614. |
the viability and apoptosis of h9c2 cells were probed by mtt assay and flow cytometry. |
2022-08-13 |
2023-08-14 |
Not clear |
| Ana Cetkovic, Alessandro Bellapianta, Mihai Irimia-Vladu, Jakob Hofinger, Cigdem Yumusak, Andrea Corna, Markus Clark Scharber, Günther Zeck, Niyazi Serdar Sariciftci, Matthias Bolz, Ahmad Salt. In Vitro Cytotoxicity of D18 and Y6 as Potential Organic Photovoltaic Materials for Retinal Prostheses. International journal of molecular sciences. vol 23. issue 15. 2022-08-12. PMID:35955800. |
human rpe cells were put in direct and indirect contact with these materials to analyze their cytotoxicity by the mtt assay, apoptosis by flow cytometry, and measurements of cell morphology and proliferation by immunofluorescence. |
2022-08-12 |
2023-08-14 |
mouse |
| Poulami Tapadar, Ambika Pal, Nirajan Ghosal, Siddhartha Dutta, Ranjana Pa. Reactive oxygen species-dependent upregulation of death receptor, tumor necrosis factor receptor 1, is responsible for theophylline-mediated cytotoxicity in MDA-MB-231 breast cancer cells. Anti-cancer drugs. 2022-08-10. PMID:35946512. |
cell viability and apoptosis was measured in the presence or absence of tnfr1 inhibitor, r7050, using ao/etbr staining and mtt assay, respectively. |
2022-08-10 |
2023-08-14 |
Not clear |
| Jing Wang, Wang Dai, Ming Zhan. GATA3 positively regulates PAR1 to facilitate in vitro disease progression and decrease cisplatin sensitivity in neuroblastoma via inhibiting the hippo pathway. Anti-cancer drugs. 2022-08-10. PMID:35946556. |
nb cell sensitivity to cisplatin, viability, migration, invasion, cell cycle progression and apoptosis were evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (mtt) assay, wound healing assay, transwell assay and flow cytometry, respectively. |
2022-08-10 |
2023-08-14 |
Not clear |
| Vincent Harrison, Saif F Khan, Victoria Damerell, Jenna Bleloch, K N ArulJothi, Musalula Sinkala, Katie Lennard, Nicola Mulder, Bridget Calder, Jonathan Blackburn, Sharon Princ. Strongylopus grayii tadpole blastema extract exerts cytotoxic effects on embryonal rhabdomyosarcoma cells. In vitro cellular & developmental biology. Animal. 2022-08-10. PMID:35947290. |
we show using mtt assays that tad inhibited erms cell viability in a concentration-dependent manner, and phase contrast/fluorescent microscopy revealed that it induced morphological markers of senescence and apoptosis. |
2022-08-10 |
2023-08-14 |
Not clear |
| Ruijun Liu, Ruili Liu, Zhiyi Guo, Jianghao Ren, Jia Huang, Qingquan Luo, Qiang Ta. shRNA‑mediated knockdown of KNTC1 inhibits non-small-cell lung cancer through regulating PSMB8. Cell death & disease. vol 13. issue 8. 2022-08-06. PMID:35933405. |
the effects of kntc1 depletion on nsclc cell proliferation, migration, apoptosis, and tumor formation were analyzed by mtt assay, wound-healing assay, transwell assay, flow cytometry assay, and in nude mouse models in vivo. |
2022-08-06 |
2023-08-14 |
mouse |
| Zahra Gharari, Parichehr Hanachi, Hanie Sadeghinia, Tony R Walke. Cichorium intybus bio-callus synthesized silver nanoparticles: a promising antioxidant, antibacterial and anticancer compound. International journal of pharmaceutics. 2022-08-02. PMID:35917872. |
the ci-agnps were investigated for their anticancer activities on the cancerous mda-mb231 cells viability and apoptosis using mtt and flow cytometry, respectively. |
2022-08-02 |
2023-08-14 |
human |
| Wei Qin, Jing Zhang, Ruixue Rong, Litao Zhang, Huijie Gao, Chao Liu, Qiang Ren, Gongpu Zheng, Jian Wang, Lingxin Meng, Sen Qiao, Ruikun Qian, Caiju Zhou, Huiyun Wang, Yuntao Zhan. Osteoglycin (OGN) promotes tumorigenesis of pancreatic cancer cell via targeting ID4. Tissue & cell. vol 78. 2022-07-31. PMID:35908351. |
the effects of ogn on cell proliferation and apoptosis were analyzed using mtt, cck8, edu and tunel assays. |
2022-07-31 |
2023-08-14 |
human |
| Hamed Manoochehri, Akram Jalali, Hamid Tanzadehpanah, Amir Taherkhani, Rezvan Najaf. Aptamer-conjugated nanoliposomes containing COL1A1 siRNA sensitize CRC cells to conventional chemotherapeutic drugs. Colloids and surfaces. B, Biointerfaces. vol 218. 2022-07-29. PMID:35905589. |
cell viability, chemosensitivity and apoptosis were determined by mtt assay and annexin/pi kit. |
2022-07-29 |
2023-08-14 |
Not clear |
| Janani Ramesh, Rejani Chalikkaran Thilakan, Raja Mohan Gopalakrishnan, Singaravel Vijayapoopathi, Arianna Dorschel, Bhuvarahamurthy Venugopa. Ginsenoside Rg5 Sensitizes Paclitaxel-Resistant Human Cervical-Adeno-Carcinoma Cells to Paclitaxel-And Enhances the Anticancer Effect of Paclitaxel. Genes. vol 13. issue 7. 2022-07-27. PMID:35885925. |
mtt assay was carried out to assess whether grg5 can potentiate the cytotoxic effect of ptx in ptx- resistant hela cells; using flow cytometry-based annexin v-fitc assay, cellular apoptosis was analyzed; the rate of expression of the cell cycle, apoptosis and major cell-survival-signaling-related genes and its proteins were examined using rt-pcr and western blotting technique. |
2022-07-27 |
2023-08-14 |
human |
| Neera Yadav, Amit Kumar Tripathi, Amna Parvee. PLGA-Quercetin Nano-Formulation Inhibits Cancer Progression via Mitochondrial Dependent Caspase-3,7 and Independent FoxO1 Activation with Concomitant PI3K/AKT Suppression. Pharmaceutics. vol 14. issue 7. 2022-07-27. PMID:35890222. |
the cell viability was assessed through mtt assay and apoptosis was assayed through hoechst-pi and eb/ao double staining followed by mitochondrial damage through mito-tracker rmx-ros. |
2022-07-27 |
2023-08-14 |
human |
| Tingrui Ge, Yonggang Zhan. Tanshinone IIA reverses oxaliplatin resistance in colorectal cancer through microRNA-30b-5p/AVEN axis. Open medicine (Warsaw, Poland). vol 17. issue 1. 2022-07-27. PMID:35892081. |
the cell proliferation and apoptosis were examined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (mtt) and flow cytometry assays. |
2022-07-27 |
2023-08-14 |
Not clear |
| Jiangqi Xu, Xiaohong Huang, Shixiang Liu, Dongdong Chen, Yufang Xie, Zhenwu Zha. The protective effects of lncRNA ZFAS1/miR-421-3p/MEF2C axis on cerebral ischemia-reperfusion injury. Cell cycle (Georgetown, Tex.). 2022-07-26. PMID:35880950. |
overexpressed or silenced plasmids of zfas1 were transfected into the cells to analyze the effects of oxygen-glucose deprivation/reperfusion (ogd/r) treatment on the viability, apoptosis and related gene expressions of neuro-2a cell by performing mtt assay, flow cytometry, qrt-pcr, and western blot. |
2022-07-26 |
2023-08-14 |
mouse |
| Bo Min, Yan Wang, Feng Liang, Cheng-Xiang Wang, Fan Wang, Zhi Yan. The Protective Mechanism of Afuresertib against Esophageal Cancer. Disease markers. vol 2022. 2022-07-25. PMID:35872696. |
in this study, we determined the effect of different concentrations of afuresertib on cell viability by mtt assay and determined the effect of afuresertib on cell apoptosis by annexin v-fitc/pi dual staining. |
2022-07-25 |
2023-08-14 |
rat |
| Marianna Oláh, Enikő Farkas, Inna Székács, Robert Horvath, András Székác. Cytotoxic effects of Roundup Classic and its components on NE-4C and MC3T3-E1 cell lines determined by biochemical and flow cytometric assays. Toxicology reports. vol 9. 2022-07-25. PMID:35875257. |
higher sensitivity differences were detected in the viability test by flow cytometry (7-9-fold), than by the mtt assay (1.5-3-fold); in the genotoxicity test by the comet assay (3.5-403-fold), than by the dna-damage test (9.3-158-fold); and in the apoptosis test by the annexin v dead cell kit (1.1-12.7-fold), than by the caspase 3/7 kit (1-6.5-fold). |
2022-07-25 |
2023-08-14 |
Not clear |
| Mostafa Ghorban Khan Tafreshi, Zohreh Mazaheri, Mansour Heidari, Nahid Babaei, Abbas Doost. Induction of Apoptosis in the U937 Cell Line Co-cultured with Adipose-derived Stem Cells Secreting Bone Morphogenetic Protein-4. International journal of molecular and cellular medicine. vol 10. issue 4. 2022-07-25. PMID:35875334. |
the induction of cell death and apoptosis in the u937 cell line was assessed using mtt and annexin v/ pi assays, respectively. |
2022-07-25 |
2023-08-14 |
human |
| Yei-Jin Kang, Won-Geun Yang, Weon-Sik Chae, Dae-Won Kim, Seong-Gon Kim, Horatiu Rotar. Administration of 4‑hexylresorcinol increases p53‑mediated transcriptional activity in oral cancer cells with the p53 mutation. Oncology reports. vol 48. issue 3. 2022-07-20. PMID:35856441. |
to determine the effect of 4hr on p53 mutant carcinoma cells, western blot analysis, p53 transcriptional activity analysis, mtt assay and apoptosis immunocytochemistry were performed. |
2022-07-20 |
2023-08-14 |
human |
| Zimin Li, Junzheng Zheng, Jian Xu, Quan Ni. Knockdown of Circ_0000798 Impedes Cell Growth and Motility of Renal Cell Carcinoma Cells Through Functioning as miRNA Sponge for miR-589-5p. Biochemical genetics. 2022-07-20. PMID:35857217. |
in vitro assays, including mtt, edu, transwell, and plate colony, scratch wound, apoptosis, and cell cycle assays, and in vivo xenograft tumor model were launched to measure cell dysfunctions. |
2022-07-20 |
2023-08-14 |
Not clear |
| Mei-Zhu Wu, Yun Xiang, Cui-Yu Bao, Tao Li. [Protective effect of salidroside on high fat-induced apoptosis in H9c2 cardiomyocytes through AMPK/mTOR/p70S6K pathway]. Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica. vol 47. issue 14. 2022-07-19. PMID:35850842. |
the study explored the effect of salidroside(sal) on high fat-induced apoptosis in h9 c2 cardiomyocytes based on ampk/mtor/p70 s6 k pathway.h9 c2 cardiomyocytes were cultured in vitro and the lipotoxicity model of h9 c2 cardiomyocytes was constructed by 0.2 mmol·l~(-1) palmitic acid(pa) treatment for 24 hours.the cells were divided into control group, pa group, and sal group(20 μmol·l~(-1)).cell proliferation was detected with cell proliferation kit i(mtt) assay after sal and pa treatment.dihydroethidium(dhe) probe, annexin v-fitc/pi kit, and jc-1 probe were used to estimate reactive oxygen species(ros) level, cell apoptosis, and mitochondrial membrane potential(mmp) change, respectively.the expression levels of p-ampk/ampk, p-mtor/mtor, p-p70 s6 k/p70 s6 k and apoptosis-related proteins bax, bcl-2, and cleaved caspase-3 were investigated with western blot.the mrna levels of ampk, mtor and p70 s6 k were determined by quantitative reverse transcription-polymerase chain reaction(qrt-pcr).results:: showed that compared with control group, pa group had decreased cell proliferation ability, mmp, bcl-2 protein expression and ampk protein and mrna expression, while increased ros level, bax and cleaved caspase-3 protein expression, and mtor and p70 s6 k mrna and protein expression, and the difference was statistically significant(p<0.05, p<0.01).compared with pa group, sal improved cell proliferation ability, mmp level, bcl-2 protein expression, and ampk mrna and protein expression, while down-regulated ros level, cell apoptosis, bax and cleaved caspase-3 protein expression, and mtor and p70 s6 k mrna and protein expression, and the difference was statistically significant(p<0.05, p<0.01).in conclusion, sal exerted protective effects on high fat-induced lipotoxicity of h9 c2 cardiomyocytes, alleviated the oxidative stress injury and reduced cell apoptosis via regulating ampk/mtor/p70 s6 k signaling pathway. |
2022-07-19 |
2023-08-14 |
Not clear |