| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| G K Jayaprakasha, Y Jadegoud, G A Nagana Gowda, Bhimanagouda S Pati. Bioactive compounds from sour orange inhibit colon cancer cell proliferation and induce cell cycle arrest. Journal of agricultural and food chemistry. vol 58. issue 1. 2010-03-22. PMID:20000570. |
cell proliferation, arrest of the cell cycle, and induction of apoptosis were assessed by mtt assay, flow cytometry, and nuclear staining methods, respectively. |
2010-03-22 |
2023-08-12 |
human |
| Stéphane Pédeboscq, Béatrice L'Azou, Isabelle Passagne, Francesca De Giorgi, François Ichas, Dominique Liguoro, Jean-Paul Pometan, Jean Camba. Anticancer drugs exert differential apoptotic and cytotoxic effects on glioblastoma primary cultures with various EGFR and bcl-2 profiles. Journal of experimental therapeutics & oncology. vol 8. issue 2. 2010-03-19. PMID:20192117. |
the cytotoxic effects of drugs were determined by mtt (dimethylthiazolyl diphenyl tetrazolium bromide) assay and apoptosis was evaluated by measuring mitochondrial potential by flow cytometry. |
2010-03-19 |
2023-08-12 |
Not clear |
| Ning Zhang, Zhen-Ming Wu, Eileen McGowan, Jun Shi, Zu-Bei Hong, Chuan-Wei Ding, Pu Xia, Wen D. Arsenic trioxide and cisplatin synergism increase cytotoxicity in human ovarian cancer cells: therapeutic potential for ovarian cancer. Cancer science. vol 100. issue 12. 2010-03-18. PMID:19769630. |
ato-mediated growth inhibition and apoptosis in human suspension ovarian cancer coc1 cells were evaluated by mtt assay and annexin v assay using flow cytometry, respectively. |
2010-03-18 |
2023-08-12 |
human |
| Wen-jian Zhang, Lian-qiu Wu, Hong-lin Liu, Li-ya Ye, Yu-ling Xin, Georges E Grau, Jin-ning Lo. Abnormal blood vessels formed by human liver cavernous hemangioma endothelial cells in nude mice are suitable for drug evaluation. Microvascular research. vol 78. issue 3. 2010-03-04. PMID:19729028. |
in addition, by mtt assay, the drug podophyllotoxin was found inhibiting hchec viability, and by tunel and dna ladder assays, podophyllotoxin was found inducing apoptosis of hchec. |
2010-03-04 |
2023-08-12 |
mouse |
| Yingjiao Yu, Siyang Zhang, Xuemei Wang, Zhiwei Yang, Guocheng O. Overexpression of TrkB promotes the progression of colon cancer. APMIS : acta pathologica, microbiologica, et immunologica Scandinavica. vol 118. issue 3. 2010-03-02. PMID:20132184. |
to investigate the effects of trkb on the progression of colon cancer, sirna specific for trkb was transfected into lovo cells, and proliferation, apoptosis and invasion of transfected cells were examined using mtt [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide], flow cytometry and transwell assays, respectively. |
2010-03-02 |
2023-08-12 |
Not clear |
| Shuji Nakagawa, Yuji Arai, Osam Mazda, Tsunao Kishida, Kenji A Takahashi, Kei Sakao, Masazumi Saito, Kuniaki Honjo, Jiro Imanishi, Toshikazu Kub. N-acetylcysteine prevents nitric oxide-induced chondrocyte apoptosis and cartilage degeneration in an experimental model of osteoarthritis. Journal of orthopaedic research : official publication of the Orthopaedic Research Society. vol 28. issue 2. 2010-02-18. PMID:19725096. |
cell viability was assessed by mtt assay, while apoptosis was evaluated by hoechst 33342 and tunel staining. |
2010-02-18 |
2023-08-12 |
rat |
| Hui Liu, Tiantuo Zhang, Benquan Wu, Jing Huang, Yuqi Zhou, Jiaxin Zh. Chronic exposure to exogenous matrilysin induces chemoresistance and enhances Bcl-2 expression in A549 lung adenocarcinoma cells. Molecular biology reports. vol 36. issue 8. 2010-02-02. PMID:19107575. |
the viability of a549 lung adenocarcinoma cells was determinated by mtt and the apoptosis was assessed by hoechst 33342 staining and annexin v-fitc/pi apoptosis kit. |
2010-02-02 |
2023-08-12 |
Not clear |
| Hao Peng, Hui Lv, Ying Wang, Yan-hong Liu, Chun-yang Li, Liang Meng, Fang Chen, Jin-ku Ba. Clematis montana lectin, a novel mannose-binding lectin from traditional Chinese medicine with antiviral and apoptosis-inducing activities. Peptides. vol 30. issue 10. 2010-02-01. PMID:19577602. |
furthermore, cml specially induced l929 cell apoptosis in dose-dependent manner as evidenced by mtt, fluorescent microscopy, ldh activity-based cytotoxicity assays and dna ladder. |
2010-02-01 |
2023-08-12 |
Not clear |
| Fen Guo, Zhi-Wen Luo, Zhao-Yu Liu, Yue-Qin Li, Hong-Jian Li, Tian-Hong Zho. [Studies of effect of prosaposin on cell proliferation, cell apoptosis and its possible molecular mechanism]. Yi chuan = Hereditas. vol 31. issue 12. 2010-02-01. PMID:20042390. |
mtt assay and annexin v/pi apoptosis kit were used to detect the effect of prosaposin on cell proliferation and cell apoptosis induced by se-rum-starvation stress, respectively. |
2010-02-01 |
2023-08-12 |
Not clear |
| Liang Xia, Min Zhao, Mei X. [Experimental investigation of pEGFP-bFGF gene transfer to human limbal stem cells]. Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi. vol 26. issue 1. 2010-01-28. PMID:19334574. |
the cellular viability in each group was measured by mtt colorimetry, and the cellular apoptosis rate and necrosis rate were observed by laser scanning confocal microscopy. |
2010-01-28 |
2023-08-12 |
human |
| Jing Shen, Wei-Jing Zhang, Yan-Chin Tai, Ching-Ho Stephen Wong, Zhigang Xie, Chien-Shing Che. [Synergistic antileukemic effect of phytoestrogens and chemotherapeutic drugs on leukemic cell lines in vitro]. Zhongguo shi yan xue ye xue za zhi. vol 16. issue 2. 2010-01-21. PMID:18426648. |
cell viability, apoptosis induction and cell cycle arrest were detected by trypan blue staining, mtt assay, optical microscopy, flow cytometry (fcm). |
2010-01-21 |
2023-08-12 |
Not clear |
| Xue-Lan Zuo, Ying Zhou, Rui-Fang Li, Ying-Qian Feng, Li He, Ming-Hui Li. [Relation of apoptosis of K562 cells induced by naringenin in vitro to enzyme activity changes of caspase-3 and caspase-8 and expression of FAS/FASL proteins]. Zhongguo shi yan xue ye xue za zhi. vol 16. issue 2. 2010-01-21. PMID:18426650. |
the inhibition of ngen on k562 cells was evaluated by means of mtt assay so as to observe the cytotoxicity of ngen; the morphological changes of the cells treated by ngen were observed by transmission electron microscope; cell apoptosis rate influenced by ngen was assessed by flow cytometry; the enzyme activity changes of caspase-3 and caspase-8 in the process of ngen-induced k562 apoptosis were detected by caspase colorimetric assay kit; immunohistochemistry technique was used to detect fas, fasl protein expression in k562 cells. |
2010-01-21 |
2023-08-12 |
Not clear |
| Hao-Bo Huang, Rong Zha. [Effect of VEGF antisense RNA on inducing apoptosis of myeloma cells and inhibition of angiogenesis in endothelial cells in vitro]. Zhongguo shi yan xue ye xue za zhi. vol 16. issue 2. 2010-01-21. PMID:18426655. |
the viability and apoptosis of u266 cells were observed by mtt assay, flow cytometry. |
2010-01-21 |
2023-08-12 |
human |
| Bruno C Cavalcanti, Daniel P Bezerra, Hemerson I F Magalhães, Manoel O Moraes, Mary Anne S Lima, Edilberto R Silveira, Cláudio A G Câmara, Vietla S Rao, Cláudia Pessoa, Letícia V Costa-Lotuf. Kauren-19-oic acid induces DNA damage followed by apoptosis in human leukemia cells. Journal of applied toxicology : JAT. vol 29. issue 7. 2010-01-21. PMID:19391104. |
these assays revealed that ka primarily intercalates with dna and not with topoisomerase i. fluorescence microscopy using ao/eb (acridine orange/ethidium bromide) staining indicated that ka can induce both apoptosis and necrosis in hl-60 cell cultures, which corroborate the findings with mtt. |
2010-01-21 |
2023-08-12 |
mouse |
| Jin Shao, Xiangyang Xu, Guosong Li, Wei Zhang, Jin Qi, Jinshen Wang, Ming Cai, Lun Cui, Lianfang Zhang, Qiang Zhao, Lianfu Den. Inhibitory effects of pharmacological doses of dexamethasone on mineralization of mesenchymal progenitor cells in vitro. Die Pharmazie. vol 64. issue 10. 2010-01-21. PMID:19947171. |
in addition, dex treatment led to inhibition of cell proliferation and a decrease of cell numbers as assessed by brdu incorporation and mtt methods, while it increased apoptosis as shown by flow cytometry of annexin v-stained cells. |
2010-01-21 |
2023-08-12 |
Not clear |
| Lan Wu, Ji-Hong Lin, Kai Bao, Peng-Fei Li, Wei-Ge Zhan. In vitro effects of erythromycin on RANKL and nuclear factor-kappa B by human TNF-alpha stimulated Jurkat cells. International immunopharmacology. vol 9. issue 9. 2010-01-14. PMID:19500694. |
we also examined proliferation of jurkat t cells by mtt assay, apoptosis by flow cytometry analysis after staining with pi and morphological observation after ao/eb staining. |
2010-01-14 |
2023-08-12 |
human |
| Jia Rao, Rong-Yan Zhang, Yan Chen, Guo-An Chen, Cheng-Jing Xia. [Combined effect of TNF-related apoptosis induced ligand and Ara-C in inducing apoptosis of HL-60 cells and its mechanism]. Zhongguo shi yan xue ye xue za zhi. vol 16. issue 3. 2010-01-12. PMID:18549619. |
the cytotoxic effect was measured by mtt assay; cell apoptosis rate was determined by flow cytometry after annexin v/pi staining; the expression level of dr5 on surface of hl-60 cells treated with ara-c at different concentrations for 24 hours was determined by flow cytometry. |
2010-01-12 |
2023-08-12 |
human |
| Guo-Guang Wang, Min Xu, Hai-Feng Duan, Gen-Bao Zhan. [Apoptosis of K562 cells induced by extract of Agkistrodon Halys' venom]. Zhongguo shi yan xue ye xue za zhi. vol 16. issue 3. 2010-01-12. PMID:18549620. |
the inhibition of k562 cell proliferation was measured by mtt assay; the morphologic changes of k562 cells was observed by microscopy; the apoptosis of k562 cells was measured by flow cytometry; the activity of extracellular signal-regulated kinase (erk) in k562 cells was detected by western blot. |
2010-01-12 |
2023-08-12 |
Not clear |
| Xiang-Liang Yuan, Bei Zhang, Li-Min Jiang, Li-Song She. [Effect of pax5 gene blocked by RNAi on biological characteristics of B cell malignant lymphoma]. Zhongguo shi yan xue ye xue za zhi. vol 16. issue 4. 2010-01-12. PMID:18718064. |
to detect the biological characteristics of pax5-silenced hematological tumor cells, the immunological phenotype, apoptosis and cell proliferation were measured by using real-time rt-pcr, mtt assay and flow cytometry respectively. |
2010-01-12 |
2023-08-12 |
Not clear |
| Hai-Yan Hu, Zhao-Yang Song, Lan Deng, Mei-Xia Zhan. Immunoregulatory effect of evodiamine in mice of various germlines. Zhongguo shi yan xue ye xue za zhi. vol 16. issue 4. 2010-01-12. PMID:18718084. |
cell proliferation was detected by mtt method, while the concentration of il-2 was detected by elisa, mrna levels of bcl-2 and cdk2 in cells treated with evodiamine were detected by rt-pcr, the apoptosis rate and intracellular reactive oxygen species (ros) concentration were analyzed by fcm, and the protein levels of bcl-2, cdk2 and bax were determined by fluorescence microscope. |
2010-01-12 |
2023-08-12 |
mouse |