| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Wei Zhang, Yan-Dong Wang, Yong-Jun Xing, Peng-Jun Liu, Jian-Hui Yan. Silencing of circ-NT5C2 retards the progression of IL-1β-induced osteoarthritis in an in vitro cell model by targeting miR-142-5p/NAMPT axis. Microbiology and immunology. 2022-12-21. PMID:36540014. |
the viability, proliferation, apoptosis and inflammation were examined using 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (mtt) assay, 5-ethynyl-2'-deoxyuridine (edu) assay, flow cytometry, and enzyme-linked immunosorbent assay (elisa), respectively. |
2022-12-21 |
2023-08-14 |
Not clear |
| Fatemeh Javaheri-Ghezeldizaj, Maryam Ghaffari, Jafar Ezzati Nazhad Dolatabadi, Parvin Dehgha. In vitro safety assessment of alkyl lactate esters in human umbilical vein endothelial cells (HUVECs). Toxicology reports. vol 10. 2022-12-20. PMID:36536829. |
the cytotoxicity effect of these additives was determined by mtt and annexin v-fitc apoptosis assays. |
2022-12-20 |
2023-08-14 |
human |
| Ning-Xin Zhang, Jun-Feng Ma, Si-Qi Li, Zhe Yin, Li L. Down-regulation of MDM2 by small interference RNA induces apoptosis and sensitizes MCF-7 breast cells to resveratrol. Chemical biology & drug design. 2022-12-14. PMID:36515454. |
proliferation and apoptosis were evaluated by mtt assay and cell death elisa assay, respectively. |
2022-12-14 |
2023-08-14 |
mouse |
| Guojing Sun, Jingwei Lu, Zhenfang Wu, Xian Zhang, Yunfan T. STAT3 expression in patients with fragility fractures and its effect on the biological function of osteoblasts. Cell and tissue banking. 2022-12-12. PMID:36508120. |
transfection of stat3 mrna mimics resulted in increased stat3 mrna expression, inhibited cell proliferation as detected by an mtt assay, and increased apoptosis rate, which was determined using flow cytometry with human fetal osteoblastic cell line 1.19 cells. |
2022-12-12 |
2023-08-14 |
human |
| Wang Li, Zhou Ping, Gao Xuemei, Meng Hongjuan, He Yi, Liu Xiaoli, Zhu Zhongxian. Chlorogenic acid regulates the proliferation and migration of high-grade serous ovarian cancer cells through modulating the miR199a5p/DDR1 axis. Acta biochimica Polonica. 2022-12-12. PMID:36508480. |
the mtt assay was used to assess the optimum concentrations of cga on the ovarian cancer cell lines ovca433 and skov3, followed by the rate of apoptosis using annexin v-fitc/pi. |
2022-12-12 |
2023-08-14 |
Not clear |
| Nan Zheng, Shan Liu, Huiting Zeng, Huajun Zhao, Lixu Ji. Molecular Mechanism of Curcumin Derivative on YAP Pathway against Ovarian Cancer. Journal of clinical medicine. vol 11. issue 23. 2022-12-11. PMID:36498794. |
mtt assay was used to detect the effect of wz10 on the proliferation of ovarian cancer ovcar3 cells; annexin v/pi double staining flow cytometry was used to detect the effect of wz10 on cell apoptosis; transwell method was used to detect the effect of wz10 on cell invasiveness; western blot was used to investigate the effect of wz10 mechanisms affecting ovcar3 activity in ovarian cancer in vitro. |
2022-12-11 |
2023-08-14 |
Not clear |
| Ayat Moradipour, Hassan Dariushnejad, Changiz Ahmadizadeh, Hamed Esmaiil Lashgaria. Dietary flavonoid carvacrol triggers the apoptosis of human breast cancer MCF-7 cells via the p53/Bax/Bcl-2 axis. Medical oncology (Northwood, London, England). vol 40. issue 1. 2022-12-10. PMID:36495389. |
after preparation and cultivation of mcf-7 cells, the ic50 value of carvacrol on the cells was evaluated by mtt assay, and then apoptosis induction was observed in the cell line treated with different concentrations of carvacrol by dapi staining. |
2022-12-10 |
2023-08-14 |
human |
| Haibing Gao, Xiangmei Wang, Huaxi Ma, Shenglong Lin, Dongqing Zhang, Wenjun Wu, Ziyuan Liao, Mengyun Chen, Hanhui Ye, Qin Li, Minghua Lin, Dongliang L. LncRNA CCAT2, involving miR-34a/TGF-β1/Smad4 signaling, regulate hepatic stellate cells proliferation. Scientific reports. vol 12. issue 1. 2022-12-09. PMID:36482069. |
the effects of ccat2 and mir-34a-5p on cell proliferation and apoptosis were verified by mtt assay, colony formation assay, and flow cytometry assay. |
2022-12-09 |
2023-08-14 |
Not clear |
| Tao Li, Hong Ge, Qingyan Yang, Junmei Wang, Qian Yin, Hongbin Wang, Gaolei Ho. Oncogenic role of microRNA-19b-3p-mediated SOCS3 in glioma through activation of JAK-STAT pathway. Metabolic brain disease. 2022-12-09. PMID:36484970. |
cell senescence was assessed by β-galactosidase staining, proliferation by mtt assay and apoptosis by flow cytometry following gain- and/or loss-of-function of mir-19b-3p or socs3. |
2022-12-09 |
2023-08-14 |
mouse |
| Yang Yang, Xiaoli Zheng, Peizan Ni, Dingjie Li, Qinfu Dan, Xiaohui Wang, Yunhan Wang, Yanan Sun, Kangdong Liu, Zigang Dong, Hong G. Targeting the STAT5A/IDO1 axis overcomes radioresistance and reverses the immunosuppressive tumor microenvironment in NSCLC. International journal of oncology. vol 62. issue 1. 2022-12-01. PMID:36453241. |
mtt assay, immunofluorescence, apoptosis analysis, cell cycle analysis, and c57bl/6 and balb/c nude mouse tumor models were utilized to evaluate the roles of the stat5a/ido1/kynurenine axis in radioresistance and in the immune microenvironment of nsclc. |
2022-12-01 |
2023-08-14 |
mouse |
| Amir-Reza Javanmard, Amin Jahanbakhshi, Hossein Nemati, Seyed Javad Mowla, Bahram M Soltan. ADAMTS9-AS1 Long Non‑coding RNA Sponges miR‑128 and miR-150 to Regulate Ras/MAPK Signaling Pathway in Glioma. Cellular and molecular neurobiology. 2022-11-30. PMID:36449154. |
at the cellular level, adamts9-as1 overexpression brought about reduced sub-g1 cell population, increased proliferation rate, reduced apoptosis level, increased migration rate, shortened bax/bcl2 ratio, induced emt, and stemness characteristics of transfected cells, detected by flow cytometry, mtt assay, scratch test, and rt-qpcr. |
2022-11-30 |
2023-08-14 |
Not clear |
| Zhang-Yong Ren, Bing Pan, Fang-Fei Wang, Shao-Cheng Lyu, Qiang H. Effects of different preservation methods of human iliac veins. Cell and tissue banking. 2022-11-28. PMID:36441442. |
survival and apoptosis of vascular cell were evaluated by mtt assay and tunel fluorescence staining. |
2022-11-28 |
2023-08-14 |
human |
| Pegah Kiamehr, Minoo Shahidi, Amir Samii, Farhad Zake. Dual Effects of Resveratrol on the Expression and Secretion of Angiogenic Factors. International journal of molecular and cellular medicine. vol 11. issue 1. 2022-11-18. PMID:36397806. |
the effects of rsv on huvecs and jurkat cell viability and apoptosis were measured by mtt and annexin-v/pi methods. |
2022-11-18 |
2023-08-14 |
human |
| Fang Wen, Zhigang Tan, Dezhi Huang, Yugang Jiang, Jun Xian. LncRNA PVT1 Promotes Neuronal Cell Apoptosis and Neuroinflammation by Regulating miR-488-3p/FOXD3/SCN2A Axis in Epilepsy. Neurochemical research. 2022-11-15. PMID:36378391. |
mtt assay and tunel staining were conducted to assess cell viability and apoptosis, respectively. |
2022-11-15 |
2023-08-14 |
rat |
| Rui Zhang, Ziqi Meng, Xuwei Wu, Meihua Zhang, Zhengri Piao, Tiefeng Ji. PD-L1/p-STAT3 promotes the progression of NSCLC cells by regulating TAM polarization. Journal of cellular and molecular medicine. 2022-11-14. PMID:36372977. |
after constructing the co-culture system and conditioned medium system, the proliferation, apoptosis, metastasis, angiogenesis, emt process and stemness of nsclc were detected by mtt, flow cytometry, transwell, endothelial cell tube formation and western blot assays. |
2022-11-14 |
2023-08-14 |
Not clear |
| Chunli Li, Jiejun Chi, Hongyan Dai, Ming Liang, Yangyang Wang, Songxin Tian, Huiyan Zhu, Hai X. Salidroside attenuates cerebral ischemia/reperfusion injury by regulating TSC2-induced autophagy. Experimental brain research. 2022-11-14. PMID:36374318. |
the results of flow cytometry and 3-(4, 5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide (mtt) assays showed that sal reduced apoptosis, enhanced cell viability and protected neuronal cells from damage by decreasing lactate dehydrogenase (ldh) activity. |
2022-11-14 |
2023-08-14 |
rat |
| Miguel Alcaraz, Amparo Olivares, Marina Andreu-Gálvez, Daniel Gyingiri Achel, Ana María Mercado, Miguel Alcaraz-Saur. Paradoxical Radiosensitizing Effect of Carnosic Acid on B16F10 Metastatic Melanoma Cells: A New Treatment Strategy. Antioxidants (Basel, Switzerland). vol 11. issue 11. 2022-11-11. PMID:36358539. |
radioprotective capacity was evaluated by clonogenic cell survival, mtt, apoptosis and intracellular glutathione assays comparing radiosensitive cells (human prostate epithelium, pnt2) with radioresistant cells (murine metastatic melanoma, b16f10). |
2022-11-11 |
2023-08-14 |
human |
| Grzegorz Adamczuk, Ewelina Humeniuk, Kamila Adamczuk, Aneta Grabarska, Jarosław Dudk. 2,4-Dinitrophenol as an Uncoupler Augments the Anthracyclines Toxicity against Prostate Cancer Cells. Molecules (Basel, Switzerland). vol 27. issue 21. 2022-11-11. PMID:36364051. |
the synergistic effect of the anthracyclines and 2,4-dnp was determined using an mtt assay, apoptosis detection and a cell cycle analysis. |
2022-11-11 |
2023-08-14 |
Not clear |
| Soumya Menon, Santhoshkumar Jayakodi, Kanti Kusum Yadav, Prathap Somu, Mona Isaq, Venkat Kumar Shanmugam, Amballa Chaitanyakumar, Nagaraj Basavegowd. Preparation of Paclitaxel-Encapsulated Bio-Functionalized Selenium Nanoparticles and Evaluation of Their Efficacy against Cervical Cancer. Molecules (Basel, Switzerland). vol 27. issue 21. 2022-11-11. PMID:36364115. |
quantitative evaluation of anti-cancer properties was performed via mtt analysis, apoptosis, gene expression analysis, cell cycle arrest, and over-production of ros. |
2022-11-11 |
2023-08-14 |
Not clear |
| Zhe Li, Yan-Long Rong, Yuan-Shi Zhan. MiR-33-5p alleviates spinal cord injury in rats and protects PC12 cells from lipopolysaccharide-induced apoptosis. The Kaohsiung journal of medical sciences. 2022-11-10. PMID:36354186. |
moreover, as mtt assays and flow cytometry showed, the suppressive effects of mir-33-5p upregulation on cell apoptosis were attenuated by rps6kb1 upregulation. |
2022-11-10 |
2023-08-14 |
rat |