| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Hongyan Wang, Jun Wang, Jing Zhang, Shuguang Jin, Huan L. Role of PI3K/AKT/mTOR signaling pathway in DBP-induced apoptosis of testicular sertoli cells in vitro. Environmental toxicology and pharmacology. vol 53. 2017-11-27. PMID:28578144. |
mtt results showed that dbp can significantly reduce the survival rate of sertoli cells; hoechst staining results showed that the sertoli cells treated with dbp emerged with typical morphological characteristics of apoptosis, nuclear condensation and chromatin condensation; flow cytometry results showed that dbp significantly increased the apoptotic rate of sertoli cells, and dose-dependent; western blotting showed that the expression of pten protein in sertoli cells was significantly higher than that in the control group after treated with different concentrations of dbp for 24h, while the expression of p-pi3k1, p-akt, p70s6k and 4e-bp1 protein in the pi3k/akt/mtor signal pathway were significantly decreased. |
2017-11-27 |
2023-08-13 |
rat |
| Yan Zhong, Yi Xu, Ai-Xia Zhang, Xiao-Feng Li, Zhao-Ying Xu, Ping Li, Bin W. Synthesis and biological evaluation of aryloxyacetamide derivatives as neuroprotective agents. Bioorganic & medicinal chemistry letters. vol 26. issue 10. 2017-11-22. PMID:27038495. |
mtt assay, hoechst 33342/pi double staining, and high content screening (hcs) revealed that pretreatment of the cells with 10m, 10r, 14b and 14c has significantly decreased the extent of cell apoptosis in a dose-dependent manner. |
2017-11-22 |
2023-08-13 |
rat |
| Yonghong Tan, Qiong Wang, Baisong Zhao, Yingjun She, Xiaobao B. GNB2 is a mediator of lidocaine-induced apoptosis in rat pheochromocytoma PC12 cells. Neurotoxicology. vol 54. 2017-11-17. PMID:27018092. |
using mtt assay and flow cytometry, we found lidocaine significantly decreased the cell proliferation and s phases in pc12 cells with treatment concentrations, and significantly enhanced cell apoptosis with treatment concentrations. |
2017-11-17 |
2023-08-13 |
rat |
| Z W Zhang, H Li, S S Chen, Y Li, Z Y Cui, J M. MicroRNA-122 regulates caspase-8 and promotes the apoptosis of mouse cardiomyocytes. Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas. vol 50. issue 2. 2017-11-13. PMID:28177059. |
cell viability and apoptosis post-transfection were assessed by mtt assay and flow cytometry, respectively. |
2017-11-13 |
2023-08-13 |
mouse |
| Sha Liu, Guozhen Gao, Dexiong Yan, Xiangjun Chen, Xingwei Yao, Shuzhong Guo, Guirong Li, Yu Zha. Effects of miR-145-5p through NRAS on the cell proliferation, apoptosis, migration, and invasion in melanoma by inhibiting MAPK and PI3K/AKT pathways. Cancer medicine. vol 6. issue 4. 2017-11-10. PMID:28332309. |
after transfecting mir-145-5p mimics, mir-145-5p inhibitors, nras cdna and nras sirna into chl-1, vmm917 and sk-mel-28 cells, functional assays were used to detect the proliferation, apoptosis, invasion and migration, including mtt, flow cytometry, transwell and wound healing assays. |
2017-11-10 |
2023-08-13 |
mouse |
| Halimatu S Mohammed, Brittany L Snyder, Damien S K Samways, Devon A Ship. Quantitative and qualitative toxicological evaluation of thiol-ene "click" chemistry-based polyanhydrides and their degradation products. Journal of biomedical materials research. Part A. vol 104. issue 8. 2017-11-06. PMID:27012532. |
apoptosis was evaluated quantitatively and qualitatively using mtt assay and fluorescence microscopic imaging as indication of cytotoxicity. |
2017-11-06 |
2023-08-13 |
mouse |
| Chiara Santulli, Claudia Brizi, Matteo Micucci, Ambra Del Genio, Assunta De Cristofaro, Federica Bracco, Giuseppina Lucia Pepe, Ilaria di Perna, Roberta Budriesi, Alberto Chiarini, Maria Frosin. Castanea sativa Mill. Bark Extract Protects U-373 MG Cells and Rat Brain Slices Against Ischemia and Reperfusion Injury. Journal of cellular biochemistry. vol 118. issue 4. 2017-11-03. PMID:27739104. |
neuroprotective effects were determined by assessing cells or slices viability (mtt assay), ros formation (dcfh-da assay), apoptosis (sub g0/g1 peak), nuclear fragmentation and chromatin condensation (dapi staining) as well as changes in lysosomes and mitochondria morphology (acridine orange and rhodamine123 staining, respectively). |
2017-11-03 |
2023-08-13 |
human |
| Libo Hou, Wei Gu, Huanxi Zhu, Wei Yao, Wen Wang, Qingguo Men. Spiroplasma eriocheiris induces mouse 3T6-Swiss albino cell apoptosis that associated with the infection mechanism. Molecular immunology. vol 91. 2017-10-26. PMID:28889064. |
fcm analysis and mtt analysis clearly shown that s. eriocheiris could induce 3t6 cell apoptosis and cause cell viability decreased seriously. |
2017-10-26 |
2023-08-13 |
mouse |
| Ziming Zhao, Hongyu Wang, Liao Zhang, Xifan Mei, Jing Hu, Keqiang Huan. Receptor for advanced glycation end product blockade enhances the chemotherapeutic effect of cisplatin in tongue squamous cell carcinoma by reducing autophagy and modulating the Wnt pathway. Anti-cancer drugs. vol 28. issue 2. 2017-10-25. PMID:27831944. |
we assayed the combined use of rage blockade and cisplatin effect on tca-8113 cells' viability by mtt and apoptosis rate of tca-8113 cells on rage blockade+cisplatin treatment; cisplatin alone; or rage blockade alone by flow cytometry. |
2017-10-25 |
2023-08-13 |
Not clear |
| Fei-Ting Hsu, Cho-Chin Sun, Chia-Hsing Wu, Yen-Ju Lee, Chih-Hung Chiang, Wei-Shu Wan. Regorafenib Induces Apoptosis and Inhibits Metastatic Potential of Human Bladder Carcinoma Cells. Anticancer research. vol 37. issue 9. 2017-10-25. PMID:28870913. |
effects of regorafenib on cell viability, apoptosis pathways, metastatic potential, and expression of metastatic and anti-apoptotic proteins were evaluated with the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium (mtt) assay, flow cytometry, cell migration and invasion assay, and western blotting. |
2017-10-25 |
2023-08-13 |
human |
| Elham Farhadi, Majid Safa, Ali M Sharifi, Davood Bashas. PRIMA-1 induces caspase-mediated apoptosis in acute promyelocytic leukemia NB4 cells by inhibition of nuclear factor-κB and downregulation of Bcl-2, XIAP, and c-Myc. Anti-cancer drugs. vol 28. issue 1. 2017-10-19. PMID:27548348. |
prima-1-triggered apoptosis in a dose-dependent and time-dependent manner as indicated by the mtt assay and annexin-v staining. |
2017-10-19 |
2023-08-13 |
human |
| Andrey V Boroda, Yulia O Kipryushina, Konstantin V Yakovlev, Nelly A Odintsov. The contribution of apoptosis and necrosis in freezing injury of sea urchin embryonic cells. Cryobiology. vol 73. issue 1. 2017-10-18. PMID:27364314. |
to estimate the effect of cryopreservation on sea urchin cell cultures and identify the involved cell death pathways, we analyzed cell viability (via trypan blue exclusion test, mtt assay and dapi staining), caspase activity (via flow cytometry and spectrophotometry), the level of apoptosis (via annexin v-fitc staining), and cell ultrastructure alterations (via transmission electron microscopy). |
2017-10-18 |
2023-08-13 |
Not clear |
| C M Lopes-Ramos, T C Pereira, D B Dogini, R Gilioli, I Lopes-Cende. Lithium carbonate and coenzyme Q10 reduce cell death in a cell model of Machado-Joseph disease. Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas. vol 49. issue 12. 2017-10-16. PMID:27878228. |
cell viability and apoptosis were evaluated by mtt assay and by flow cytometry after staining with annexin v-fitc/propidium iodide. |
2017-10-16 |
2023-08-13 |
Not clear |
| X Huang, Y Teng, H Yang, J M. Propofol inhibits invasion and growth of ovarian cancer cells via regulating miR-9/NF-κB signal. Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas. vol 49. issue 12. 2017-10-16. PMID:27982283. |
ovarian cancer es-2 cells were transfected with anti-mir-9 or p65 cdna or p65 sirna for 24 h, after which the cells were treated with different concentrations of propofol (1, 5, and 10 μg/ml) for 24 h. cell growth and apoptosis were detected using mtt assay and flow cytometry analysis. |
2017-10-16 |
2023-08-13 |
Not clear |
| Yuanyuan Xu, Ming Shen, Ying Sun, Pei Gao, Yourong Dua. Polymer Nanocomposites Based Thermo-Sensitive Gel for Paclitaxel and Temozolomide Co-Delivery to Glioblastoma Cells. Journal of nanoscience and nanotechnology. vol 15. issue 12. 2017-10-13. PMID:26682412. |
finally, the cytotoxic and apoptosis-inducing effects of the gel on the human malignant glioblastoma cell line u87 and c6 rat glioblastoma cell line were evaluated by mtt and flow cytometry apoptosis assay, respectively. |
2017-10-13 |
2023-08-13 |
human |
| Lifeng Wang, Yingjie Lu, Xian Liu, Xiaoyun Wan. Ghrelin protected neonatal rat cardiomyocyte against hypoxia/reoxygenation injury by inhibiting apoptosis through Akt-mTOR signal. Molecular biology reports. vol 44. issue 2. 2017-10-10. PMID:28281036. |
we isolated the nrcms, established myocardial h/r model, blocked growth hormone secretagogue receptor (ghsr) by sirna technique, examined cell activity by mtt and ldh assay, detected apoptosis by hoechst 33258 staining and flow cytometry and determined the expression levels of apoptosis related proteins and signaling pathway proteins by western blot. |
2017-10-10 |
2023-08-13 |
rat |
| Sridaran Dhivya, Nidhi Khandelwal, Suresh K Abraham, Kumpati Premkuma. Impact of Anthocyanidins on Mitoxantrone-Induced Cytotoxicity and Genotoxicity: An In Vitro and In Vivo Analysis. Integrative cancer therapies. vol 15. issue 4. 2017-10-06. PMID:27146128. |
methods cytotoxicity of test agents and apoptosis induction in hepg2 cells was assessed by mtt assay, trypan blue dye exclusion assay and hoechst 33258 staining. |
2017-10-06 |
2023-08-13 |
mouse |
| Yi Xu, Jian-Yong Zhu, Zhang-Ming Lei, Li-Jun Wan, Xiu-Wen Zhu, Feng Ye, Yan-Yue Ton. Anti-proliferative effects of paeonol on human prostate cancer cell lines DU145 and PC-3. Journal of physiology and biochemistry. vol 73. issue 2. 2017-10-04. PMID:27834040. |
cell growth was measured by mtt, and the apoptosis was detected by the flow cytometry. |
2017-10-04 |
2023-08-13 |
mouse |
| Zheng Han, Xiaodong Huang, Meng Liu, Jie Tan, Qingxi Zhu, Yanfen Wang, Yishan Cai, Aifang Chen, Xia Tia. [Knock-down of cadherin 17 inhibits proliferation and promote apoptosis in noscapine-resistant human SW480 colon cancer cells]. Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology. vol 33. issue 5. 2017-10-04. PMID:28502297. |
transfected sw480 cells were treated with noscapine, and then the proliferation and cell viability of sw480 cells were measured by mtt assay and plate clone formation assay, respectively; the apoptosis of sw480 cells was detected by flow cytometry combined with annexinv-fitc/pi staining; the expressions of poly-adp-ribose polymerase (parp) and caspase-3 were determined by western blotting. |
2017-10-04 |
2023-08-13 |
human |
| Xiaojie Huang, Tianming Lian, Xiaoqian Guan, Bin Liu, Siyuan Hao, Jingjing Zhang, Shiting Bao, Xiaoyu Tan, Runzhi Zh. Dihydromyricetin Reduces TGF-β Via P53 Activation-dependent Mechanism in Hepatocellular Carcinoma HepG2 Cells. Protein and peptide letters. vol 24. issue 5. 2017-10-02. PMID:28240161. |
in this study, we demonstrated that dhm could significantly inhibit proliferation and induce apoptosis in hepg2 cells with mtt and flow cytometry methods. |
2017-10-02 |
2023-08-13 |
human |