| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Wei Huang, Xiuwen Lan, Xueting Li, Dawei Wang, Yinghao Sun, Qian Wang, Hong Gao, Kaijiang Y. Long non-coding RNA PVT1 promote LPS-induced septic acute kidney injury by regulating TNFα and JNK/NF-κB pathways in HK-2 cells. International immunopharmacology. vol 47. 2018-03-26. PMID:28391160. |
cell viability and apoptosis rate were detected by mtt assay and annexin v-fitc/pi apoptosis detection kit, respectively. |
2018-03-26 |
2023-08-13 |
Not clear |
| Zuowen Liang, Hongliang Wang, Baofeng Guo, Fubiao Li, Jinsha Liu, Zhewen Liu, Libo Xu, Wenjing Yun, Xuejian Zhao, Ling Zhan. Inhibition of prostate cancer RM1 cell growth in vitro by hydroxyapatite nanoparticle‑delivered short hairpin RNAs against Stat3. Molecular medicine reports. vol 16. issue 1. 2018-03-26. PMID:28534932. |
mtt assays were used to measure rm1 cell viability 24 and 48 h following transfection, and the apoptosis rate and cell cycle phase distribution were determined by flow cytometry. |
2018-03-26 |
2023-08-13 |
Not clear |
| Jiann-Shing Wu, Jan-Yi Chang, C Will Chen, Ming-Tse Lin, Dey-Chyi Sheu, Shun-Mei Le. Neokestose suppresses the growth of human melanoma A2058 cells via inhibition of the nuclear factor‑κB signaling pathway. Molecular medicine reports. vol 16. issue 1. 2018-03-26. PMID:28535012. |
the cells were initially treated with neokestose; subsequently, in vitro cytotoxicity was assessed using mtt, and cell cycle progression and apoptosis were detected using flow cytometry. |
2018-03-26 |
2023-08-13 |
human |
| Y Huang, S-H Liang, L-B Xiang, X-T Han, W Zhang, J Tang, X-H Wu, M-Q Zhan. [miR-218 Promoted the Apoptosis of Human Ovarian Carcinoma Cells via Suppression of the WNT/β-Catenin Signaling Pathway]. Molekuliarnaia biologiia. vol 51. issue 4. 2018-03-23. PMID:28900081. |
after over-expressing mir-218 in human ovarian carcinoma (ovcar3) cells, cell viability was determined by mtt method, cell apoptosis was observed by flow cytometry (fcm), mrna expression of mir-218, bcl2, bax was measured by rt-pcr and protein expression levels of wnt, tankyrase and β-catenin were quantified by western blots. |
2018-03-23 |
2023-08-13 |
human |
| Sheng Wang, Xuan Zhang, Yuanyuan Guo, Han Rong, Tiebang Li. The long noncoding RNA HOTAIR promotes Parkinson's disease by upregulating LRRK2 expression. Oncotarget. vol 8. issue 15. 2018-03-22. PMID:28445933. |
mtt and flow cytometry assay were performed to measure the cell viability and apoptosis of sh-sy5y cells. |
2018-03-22 |
2023-08-13 |
mouse |
| Nicholas E Thomford, Buyisile Mkhize, Kevin Dzobo, Keleabetswe Mpye, Arielle Rowe, M Iqbal Parker, Ambroise Wonkam, Michelle Skelton, Alison V September, Collet Dandar. African Lettuce (Launaea taraxacifolia) Displays Possible Anticancer Effects and Herb-Drug Interaction Potential by CYP1A2, CYP2C9, and CYP2C19 Inhibition. Omics : a journal of integrative biology. vol 20. issue 9. 2018-03-14. PMID:27631192. |
antiproliferative and apoptotic effects were evaluated using the mtt [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay and flow cytometry, while examining, in parallel, the genes regulating apoptosis and cell cycle in this cell culture model. |
2018-03-14 |
2023-08-13 |
human |
| Linsen Feng, Jianhua Ma, Haiming Ji, Yichun Liu, Weixing H. miR-330-5p suppresses glioblastoma cell proliferation and invasiveness through targeting ITGA5. Bioscience reports. vol 37. issue 3. 2018-03-13. PMID:28336765. |
the present study used mtt assay, colony formation assay, transwell assay, wound healing assay, and flow cytometry analysis in order to determine the biological functions of gbm cells (including cell proliferation, invasion, migration, apoptosis, and cell cycle). |
2018-03-13 |
2023-08-13 |
Not clear |
| Bing Zhao, Zebao Zhen. Insulin Growth Factor 1 Protects Neural Stem Cells Against Apoptosis Induced by Hypoxia Through Akt/Mitogen-Activated Protein Kinase/Extracellular Signal-Regulated Kinase (Akt/MAPK/ERK) Pathway in Hypoxia-Ishchemic Encephalopathy. Medical science monitor : international medical journal of experimental and clinical research. vol 23. 2018-03-13. PMID:28420864. |
the cell viability and apoptosis were respectively measured by 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (mtt) assay and flow cytometry. |
2018-03-13 |
2023-08-13 |
rat |
| Liang Zhou, Jie Zhang, Chao Wang, Qiangsan Su. Tanshinone inhibits neuronal cell apoptosis and inflammatory response in cerebral infarction rat model. International journal of immunopathology and pharmacology. vol 30. issue 2. 2018-03-12. PMID:28402151. |
after pretreatment with tsn, cell viability and apoptosis were observed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (mtt) assay and flow cytometry analysis, respectively. |
2018-03-12 |
2023-08-13 |
rat |
| Mahmoud Abudayyak, Elif Guzel, Gül Özha. Nickel Oxide Nanoparticles Induce Oxidative DNA Damage and Apoptosis in Kidney Cell Line (NRK-52E). Biological trace element research. vol 178. issue 1. 2018-03-06. PMID:27878512. |
the following assays were used: the nanoparticle characterization by transmission electron microscopy (tem) and dynamic light scattering (dls); the determination of cellular uptake and morphologic changes by tem and inductively coupled plasma-mass spectrometry (icp-ms); mtt and neutral red uptake (nru) assays for cytotoxicity; comet assay for genotoxicity; the determination of malondialdehyde (mda), 8-hydroxydeoxyguanosine (8-ohdg), protein carbonyl (pc) and glutathione (gsh) levels by enzyme-linked immune sorbent assays (elisa) for the potential of oxidative damage; and annexin v-fitc apoptosis detection assay with propidium iodide (pi) for apoptosis. |
2018-03-06 |
2023-08-13 |
human |
| Fang Wang, Pengwei Lv, Yuanting Gu, Lin Li, Xin Ge, Guangcheng Gu. Galectin-1 knockdown improves drug sensitivity of breast cancer by reducing P-glycoprotein expression through inhibiting the Raf-1/AP-1 signaling pathway. Oncotarget. vol 8. issue 15. 2018-03-05. PMID:28212576. |
mtt assay and flow cytometry revealed that gal-1 knockdown improved sensitivity to paclitaxel (ptx) and adriamycin (adr) in mcf-7/ptx and mcf-7/adr cells via inhibition of cell viability and promotion of cell apoptosis, while mdr1 overexpression weakened the sensitivity to ptx and adr induced by gal-1 knockdown. |
2018-03-05 |
2023-08-13 |
Not clear |
| Ting Zhang, Ting Wang, Peiling Ca. Sclareol inhibits cell proliferation and sensitizes cells to the antiproliferative effect of bortezomib via upregulating the tumor suppressor caveolin-1 in cervical cancer cells. Molecular medicine reports. vol 15. issue 6. 2018-03-05. PMID:28440485. |
the present study analyzed cell proliferation and cell apoptosis by mtt and fitc‑annexin v assays. |
2018-03-05 |
2023-08-13 |
human |
| Yong Li, Dapeng Wu, Pei Wang, Xiaohui Li, Gongning Sh. miR-195 Regulates Proliferation and Apoptosis through Inhibiting the mTOR/p70s6k Signaling Pathway by Targeting HMGA2 in Esophageal Carcinoma Cells. Disease markers. vol 2017. 2018-03-05. PMID:28487599. |
mtt assay and flow cytometry revealed that mir-195 overexpression inhibited cell proliferation and induced apoptosis by targeting hmga2. |
2018-03-05 |
2023-08-13 |
Not clear |
| Boda Ying, Hong Huang, Hongfei Li, Meng Song, Sizhan Wu, Hongliang Yin. Procaine Inhibits Proliferation and Migration and Promotes Cell Apoptosis in Osteosarcoma Cells by Upregulation of MicroRNA-133b. Oncology research. vol 25. issue 9. 2018-03-01. PMID:28251881. |
mg63 cells were treated with different concentrations of pca, and cell viability, apoptosis, and mir-133b expression were then detected by mtt, flow cytometry, and qrt-pcr, respectively. |
2018-03-01 |
2023-08-13 |
Not clear |
| Rokas Račkauskas, Dachen Zhou, Simonas Ūselis, Kęstutis Strupas, Ingrid Herr, Peter Schemme. Sulforaphane sensitizes human cholangiocarcinoma to cisplatin via the downregulation of anti-apoptotic proteins. Oncology reports. vol 37. issue 6. 2018-03-01. PMID:28498473. |
viability, the platinated dna content, and apoptosis were assessed using both mtt assay and flow cytometry, while western blotting was used to analyze the expression of proteins involved in apoptosis and dna damage. |
2018-03-01 |
2023-08-13 |
human |
| Yuan Qin, Jing Wei, Yi Zhang, Ling Kang, Bibo Kang, Huanjun Shen, Xuyang Zheng, Yaning Wang, Zhansheng Jia, Ying Zhan. [Mechanism for the inhibition of proliferation and promotion of apoptosis in Huh7.5 cells by iron overload]. Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology. vol 33. issue 8. 2018-03-01. PMID:28871946. |
fluorescence microscopy was employed to determine cell iron load labeled by phen green fl; proliferation activity of huh7.5 cells was evaluated by mtt assay; protein and mrna levels of transferrin receptor (tfr1), tfr2, divalent metal transporter 1 (dmt1) and ferroportin 1 (fpn1) in huh7.5 cells were detected by western blotting and real-time pcr, respectively; cell reactive oxygen species (ros) labeled by dichlorofluorescin diacetate (dcfh-da) and cell apoptosis labeled by annexinv-fitc/pi were analyzed by flow cytometry. |
2018-03-01 |
2023-08-13 |
Not clear |
| Dazhong Cao, Ting Yu, Xilong O. MiR-873-5P controls gastric cancer progression by targeting hedgehog-GLI signaling. Die Pharmazie. vol 71. issue 10. 2018-02-27. PMID:29441930. |
the role of gli1 and mir-873-5p on gastric cancer cell viability, apoptosis and cell cycle was determined using mtt assay and flow cytometry, respectively. |
2018-02-27 |
2023-08-13 |
human |
| Zhigang Chen, Shaoli Zhang, Changlei Guo, Jianhua Li, Wenfeng San. Downregulation of miR-200c protects cardiomyocytes from hypoxia-induced apoptosis by targeting GATA-4. International journal of molecular medicine. vol 39. issue 6. 2018-02-26. PMID:28440427. |
the lactate dehydrogenase, mtt, annexin v/propidium iodide apoptosis and caspase-3 activity assays showed that downregulation of mir-200c markedly improved cell survival and suppressed the apoptosis of cardiomyocytes in response to hypoxia. |
2018-02-26 |
2023-08-13 |
Not clear |
| Yi-Fei Chen, Shu-Ying Wang, You-Hui Yang, Jiang Zheng, Ting Liu, Li Wan. Targeting HSF1 leads to an antitumor effect in human epithelial ovarian cancer. International journal of molecular medicine. vol 39. issue 6. 2018-02-26. PMID:28487934. |
cell proliferative activity was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (mtt) assay; cell cycle distribution and apoptosis were determined by flow cytometric analysis. |
2018-02-26 |
2023-08-13 |
mouse |
| Shuqing Li, Lina Yang, Jingshu Wang, Fan Liang, Bin Chang, Huafen Gu, Honglin Wang, Gong Yang, Yaping Che. Analysis of the chemotherapeutic effects of a propadiene compound on malignant ovarian cancer cells. Oncotarget. vol 7. issue 35. 2018-02-23. PMID:27494891. |
mtt assay showed that phpo displayed a much lower ic50 than cisplatin and paclitaxel, while combination treatment of cells with phpo + cisplatin induced more apoptosis than with phpo + paclitaxel or with cisplatin + paclitaxel (p < 0.05). |
2018-02-23 |
2023-08-13 |
Not clear |