| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Chuangui Chen, Zhao Ma, Hongdian Zhang, Xiaoqiong Liu, Zhentao Y. Krüppel-Like Factor 4 Enhances Sensitivity of Cisplatin to Esophageal Squamous Cell Carcinoma (ESCC) Cells. Medical science monitor : international medical journal of experimental and clinical research. vol 23. 2018-04-23. PMID:28694421. |
cell viability, apoptosis, and cell cycle were measured using methyl thiazolyl tetrazolium (mtt) assay, annexin v affinity assay, and flow cytometry, respectively. |
2018-04-23 |
2023-08-13 |
human |
| Wenlei Li, Guotao Jia, Yanwen Qu, Qian Du, Baoguo Liu, Bin Li. Long Non-Coding RNA (LncRNA) HOXA11-AS Promotes Breast Cancer Invasion and Metastasis by Regulating Epithelial-Mesenchymal Transition. Medical science monitor : international medical journal of experimental and clinical research. vol 23. 2018-04-23. PMID:28701685. |
material and methods in this study, fluorescent quantitative real-time pcr (qrt-pcr), mtt assay and clone formation assay, flow cytometry, transwell assay and wound healing assay, immunofluorescence, and western blot analysis were conducted to detect the expression of lncrna hoxa11-as, cell proliferation activity, cell apoptosis rate and cell cycle distribution, the changes of cell invasion and metastasis capacity, and the expressions of molecular markers of epithelial-mesenchymal transition (emt), respectively. |
2018-04-23 |
2023-08-13 |
mouse |
| Wenlei Li, Guotao Jia, Yanwen Qu, Qian Du, Baoguo Liu, Bin Li. Long Non-Coding RNA (LncRNA) HOXA11-AS Promotes Breast Cancer Invasion and Metastasis by Regulating Epithelial-Mesenchymal Transition. Medical science monitor : international medical journal of experimental and clinical research. vol 23. 2018-04-23. PMID:28701685. |
mtt assay suggested that tumor cell proliferation capacity was suppressed followed by the knockdown of lncrna hoxa11-as expression in mda-mb-231 and mcf-7 cells; flow cytometry results demonstrated that interfering in lncrna hoxa11-as could induce tumor cell apoptosis and promote cell cycle progression to be arrested in g1/g0 stage; experiments in vivo/vitro manifested that interfering in lncrna hoxa11-as could inhibit tumor cell invasion and migration capacity by affecting the expressions of emt-related molecular markers (e-cadherin, n-cadherin, vimentin). |
2018-04-23 |
2023-08-13 |
mouse |
| Yingyi Wang, Ming Zhao, Jieying Liu, Zhao Sun, Jianjiao Ni, Hongsheng Li. miRNA-125b regulates apoptosis of human non-small cell lung cancer via the PI3K/Akt/GSK3β signaling pathway. Oncology reports. vol 38. issue 3. 2018-04-23. PMID:28713974. |
proliferation and apoptosis assays were subsequently conducted in the human nsclc cell line a549 using mtt assay and annexin v-fitc/pi kits, respectively. |
2018-04-23 |
2023-08-13 |
human |
| Hailing Hou, Puchun Er, Jingjing Cheng, Xiuli Chen, Xiaofeng Ding, Yuwen Wang, Xi Chen, Zhiyong Yuan, Qingsong Pang, Ping Wang, Dong Qia. High expression of FUNDC1 predicts poor prognostic outcomes and is a promising target to improve chemoradiotherapy effects in patients with cervical cancer. Cancer medicine. vol 6. issue 8. 2018-04-23. PMID:28719148. |
mtt assays and flow cytometric analysis were applied to examine cell proliferation and apoptosis. |
2018-04-23 |
2023-08-13 |
human |
| Jinpeng Jia, Pei Yin, Gang Han, Meng Xu, Wei Wang, Wenzhi B. MicroRNA-300 decreases cell viability, inhibits migration and promotes apoptosis of osteosarcoma cells via downregulation of Twist1. Molecular medicine reports. vol 16. issue 3. 2018-04-23. PMID:28765882. |
following transfection with mir‑300 mimic, mir‑300 inhibitor or scramble control, mg63 cell viability, migration and apoptosis were respectively measured by 3‑(4,5‑dimethyl‑2‑thiazolyl)‑2,5‑diphenyltetrazolium bromide (mtt), modified two‑chamber migration assay and flow cytometry. |
2018-04-23 |
2023-08-13 |
human |
| Dongmei Su, Yunjun Zhou, Shanshan Hu, Lina Guan, Cuige Shi, Qi Wang, Yingyu Chen, Cailing Lu, Qian Li, Xu M. Role of GAB1/PI3K/AKT signaling high glucose-induced cardiomyocyte apoptosis. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie. vol 93. 2018-04-20. PMID:28738535. |
mtt and apoptosis assay showed gab1 played a key role in mediating hg-induced apoptosis of cardiomyocytes. |
2018-04-20 |
2023-08-13 |
rat |
| Jun-Wei Zhao, Hai-Lou Li, Xing Ma, Zhigang Xie, Li-Juan Chen, Yongsheng Zh. Lanthanide-Connecting and Lone-Electron-Pair Active Trigonal-Pyramidal-AsO3 Inducing Nanosized Poly(polyoxotungstate) Aggregates and Their Anticancer Activities. Scientific reports. vol 6. 2018-04-19. PMID:27193961. |
moreover, the cytotoxicity tests of 4 and 8 toward human cervical cancer (hela) cells, human breast cancer (mcf-7) cells and mouse fibroblast (l929) cells were performed by the 3-(4,5-cimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (mtt) assay and the cell apoptosis processes were characterized by calcein am/pi staining experiments, annexin v-fitc/pi staining experiments and morphological changes. |
2018-04-19 |
2023-08-13 |
mouse |
| Jing-Bo Li, Hai-Yang Wang, Ye Yao, Qing-Feng Sun, Zong-Hong Liu, Si-Qi Liu, Jun-Li Zhuang, Yun-Peng Wang, Hong-Yu Li. Overexpression of microRNA-138 alleviates human coronary artery endothelial cell injury and inflammatory response by inhibiting the PI3K/Akt/eNOS pathway. Journal of cellular and molecular medicine. vol 21. issue 8. 2018-04-17. PMID:28371277. |
mtt and flow cytometry were performed to assess the proliferation and apoptosis of hcaecs. |
2018-04-17 |
2023-08-13 |
human |
| Binbin Ma, Zebin Gao, Jiacheng Lou, Hongqiang Zhang, Zhongbo Yuan, Qiong Wu, Xinyu Li, Bo Zhan. Long non‑coding RNA MEG3 contributes to cisplatin‑induced apoptosis via inhibition of autophagy in human glioma cells. Molecular medicine reports. vol 16. issue 3. 2018-04-16. PMID:28677749. |
to investigate the role of meg3, the mrna levels of meg3 under cisplatin treatment were investigated by reverse transcription‑quantitative polymerase chain reaction, and the cell viability and apoptosis were examined by mtt assay, and flow cytometry analysis and western blotting, respectively. |
2018-04-16 |
2023-08-13 |
human |
| Jie Li, Hairong Jin, Hua Yu, Bin Wang, Jian Tan. miRNA‑1284 inhibits cell growth and induces apoptosis of lung cancer cells. Molecular medicine reports. vol 16. issue 3. 2018-04-16. PMID:28713980. |
subsequently, cell viability, growth and apoptosis of a459 cells in the mir‑1284 mimic, mir‑1284 inhibitor and control groups were assayed by mtt assay, bromodeoxyuridine assay and flow cytometry, respectively. |
2018-04-16 |
2023-08-13 |
Not clear |
| Xiaoqin Du, Wanhu Fan, Yunru Che. microRNA-520f inhibits hepatocellular carcinoma cell proliferation and invasion by targeting TM4SF1. Gene. vol 657. 2018-04-16. PMID:29505836. |
functional investigations revealed that overexpression of mir-520f significantly suppressed cell proliferation, invasion and migration, caused cell cycle arrested at g0/g1 phase, and promoted cell apoptosis in hcc cells according to mtt, colony formation, transwell, and flow cytometry assays, respectively, whereas, downregulation of mir-520f exhibited inverse effects. |
2018-04-16 |
2023-08-13 |
human |
| Mahmoud Abudayyak, Elif Guzel, Gül Özha. Nickel oxide nanoparticles are highly toxic to SH-SY5Y neuronal cells. Neurochemistry international. vol 108. 2018-04-13. PMID:28159626. |
then, the cytotoxicity was evaluated by mtt and neutral red uptake assays, the genotoxicity by comet assay, the oxidative potentials by the determination of malondialdehyde, 8-hydroxy deoxyguanosine, protein carbonyl, and glutathione levels with enzyme-linked immune sorbent assays, and the apoptotic potentials by annexin v-fitc apoptosis detection assay with propidium iodide. |
2018-04-13 |
2023-08-13 |
human |
| Yingchun Liu, Jiangguo Tang, Xiaoxiao Gao, Min Wang, Jie Shen, Xiaoqing Yo. Effect of retinoid X receptor-α nuclear export inhibition on apoptosis of neurons in vivo and in vitro. Molecular medicine reports. vol 16. issue 2. 2018-04-09. PMID:28627642. |
in addition, the apoptosis and viability of n2a cells was detected using flow cytometry and mtt assays, respectively, and the expression of b cell lymphoma 2 (bcl‑2) and bcl‑2 associated x (bax) was quantified by western blotting. |
2018-04-09 |
2023-08-13 |
mouse |
| Fen Wang, Nanfang Qu, Jin Peng, Chun Yue, Lingzhi Yuan, Yi Yua. CagA promotes proliferation and inhibits apoptosis of GES-1 cells by upregulating TRAF1/4-1BB. Molecular medicine reports. vol 16. issue 2. 2018-04-06. PMID:28627614. |
an mtt assay and flow cytometric analysis was used to assess cell viability and apoptosis, respectively. |
2018-04-06 |
2023-08-13 |
Not clear |
| Hui Liang, Ziyan Dong, Jiang-Feng Liu, Wei Chuang, Li-Zhen Gao, Yu-Guo Re. Targeting miR-155 suppresses proliferation and induces apoptosis of HL-60 cells by targeting Slug/PUMA signal. Histology and histopathology. vol 32. issue 9. 2018-04-05. PMID:27786352. |
hl-60 cells were treated with anti-mir-155 for 72 h. cell growth and apoptosis in vitro were detected by mtt, brdu proliferation, colony formation and flow cytometry assay. |
2018-04-05 |
2023-08-13 |
mouse |
| Aya Yamamura, Eri Fujitomi, Naoki Ohara, Kikuo Tsukamoto, Motohiko Sato, Hisao Yamamur. Tadalafil induces antiproliferation, apoptosis, and phosphodiesterase type 5 downregulation in idiopathic pulmonary arterial hypertension in vitro. European journal of pharmacology. vol 810. 2018-04-05. PMID:28603047. |
in the present study, the in vitro pharmacological profiles of tadalafil for cell proliferation and apoptosis were assessed in ipah-pasmcs using mtt, brdu incorporation, and caspase 3/7 assays. |
2018-04-05 |
2023-08-13 |
Not clear |
| Jian Chen, Qitian Mu, Xia Li, Xiufeng Yin, Mengxia Yu, Jing Jin, Chenying Li, Yile Zhou, Jiani Zhou, Shanshan Suo, Demin Lu, Jie Ji. Homoharringtonine targets Smad3 and TGF-β pathway to inhibit the proliferation of acute myeloid leukemia cells. Oncotarget. vol 8. issue 25. 2018-04-02. PMID:28454099. |
mtt assay was used for proliferation detection, flowcytometry was used to detect apoptosis and cell cycle arrest upon hht functioning, western blotting was used to detect the protein level changes, viral shrna transfection was used to suppress the expression level of the target protein candidate, and viral mrna transfection was used for over-expression. |
2018-04-02 |
2023-08-13 |
human |
| Yadong Wu, Xiangjie Sun, Bin Song, Xiaoling Qiu, Jianjiang Zha. MiR-375/SLC7A11 axis regulates oral squamous cell carcinoma proliferation and invasion. Cancer medicine. vol 6. issue 7. 2018-03-29. PMID:28627030. |
mtt, colony formation, transwell, wound healing assays and flow cytometry were used to detect oscc cell viability, proliferation, invasion, migration and apoptosis, respectively. |
2018-03-29 |
2023-08-13 |
Not clear |
| Geng Wang, Na Li, Xiaohong Lv, Naila Ahmed, Xinlei Li, Huidong Liu, Jing Ma, Yafang Zhan. Triptolide Suppresses Alkali Burn-Induced Corneal Angiogenesis Along with a Downregulation of VEGFA and VEGFC Expression. Anatomical record (Hoboken, N.J. : 2007). vol 300. issue 7. 2018-03-26. PMID:28233432. |
cell proliferation and apoptosis were measured by mtt assay and flow cytometry, respectively. |
2018-03-26 |
2023-08-13 |
mouse |