| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Yang Yu, Feng Lv, Dong Liang, Qinheng Yang, Bin Zhang, Hong Lin, Xiaofang Wang, Guo Qian, Jinzhong Xu, Wei Yo. HOTAIR may regulate proliferation, apoptosis, migration and invasion of MCF-7 cells through regulating the P53/Akt/JNK signaling pathway. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie. vol 90. 2018-02-23. PMID:28407576. |
mcf-7 cells were cultured and transfected with hotair sirna, and the proliferation rate of cells was determined using mtt and colony-forming assay; moreover, the apoptosis as well as cell cycles were determined using annexin v/propidium iodide staining methods and analyzed using flow cytometery; furthermore, cell scratch and transwell assays have been performed to examine the migration and invasion of mcf-7 cells; next, cells were collected, and rt-qpcr as well as western blotting assay were performed to examine the expression of p53, mdm2, akt, jnk, mmp-2 and mmp-9. |
2018-02-23 |
2023-08-13 |
human |
| Lianshun Ma, Yaping Yang, Zizhang Yin, Mei Liu, Liwei Wang, Lixin Chen, Linyan Zhu, Haifeng Yan. Emodin suppresses the nasopharyngeal carcinoma cells by targeting the chloride channels. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie. vol 90. 2018-02-23. PMID:28411554. |
the proliferation, cell cycle arrest and apoptosis of poorly differentiated human nasopharyngeal carcinoma cells (cne-2z) and normal nasopharyngeal epithelial cells (np69-sv40t) were detected by 3-(4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide(mtt)and flow cytometry. |
2018-02-23 |
2023-08-13 |
human |
| Quan Zhou, Fengli Chen, Jiali Zhao, Baojun Li, Yong Liang, Wei Pan, Shaoxian Zhang, Xinhong Wang, Donghui Zhen. Long non-coding RNA PVT1 promotes osteosarcoma development by acting as a molecular sponge to regulate miR-195. Oncotarget. vol 7. issue 50. 2018-02-22. PMID:27813492. |
mtt and clonal colony formation assays were used to detect the effect of pvt1 on proliferation, and flow cytometry was performed to assess apoptosis and the cell cycle. |
2018-02-22 |
2023-08-13 |
Not clear |
| Chun-Li Song, Bin Liu, Yong-Feng Shi, Ning Liu, You-You Yan, Ji-Chang Zhang, Xin Xue, Jin-Peng Wang, Zhuo Zhao, Jian-Gen Liu, Yang-Xue Li, Xiao-Hao Zhang, Jun-Duo W. MicroRNA-130a alleviates human coronary artery endothelial cell injury and inflammatory responses by targeting PTEN via activating PI3K/Akt/eNOS signaling pathway. Oncotarget. vol 7. issue 44. 2018-02-21. PMID:27713121. |
mtt assay and hoechst 33258 staining were adopted to testify cell growth and apoptosis. |
2018-02-21 |
2023-08-13 |
human |
| Guohui Du, Dongmei Cao, Lingzheng Men. miR-21 inhibitor suppresses cell proliferation and colony formation through regulating the PTEN/AKT pathway and improves paclitaxel sensitivity in cervical cancer cells. Molecular medicine reports. vol 15. issue 5. 2018-02-16. PMID:28447761. |
cell viability, colony formation and apoptosis were then analyzed using an mtt assay, crystal violet and annexin v-fluorescein isothiocyanate/propidium iodide staining, respectively. |
2018-02-16 |
2023-08-13 |
Not clear |
| Weipeng Zhang, Xiaowei Zheng, Ti Meng, Haisheng You, Yalin Dong, Jianfeng Xing, Siying Che. SET protein overexpression contributes to paclitaxel resistance in MCF-7/S cells through PI3K/Akt pathway. Journal of drug targeting. vol 25. issue 3. 2018-02-15. PMID:27718638. |
subsequently, cell viability to paclitaxel was assessed by mtt assay and cell apoptosis was analyzed by flow cytometry. |
2018-02-15 |
2023-08-13 |
human |
| Xin Lian, Junlian Gu, Baoshan Gao, Yan Li, Chendil Damodaran, Wei Wei, Yaowen Fu, Lu Ca. Fenofibrate inhibits mTOR-p70S6K signaling and simultaneously induces cell death in human prostate cancer cells. Biochemical and biophysical research communications. vol 496. issue 1. 2018-02-15. PMID:29305864. |
the methods used include cell viability analysis with an mtt assay, as well as apoptosis and related signaling pathway analyses with flow cytometry and western blotting. |
2018-02-15 |
2023-08-13 |
human |
| Elham Kashafi, Maliheh Moradzadeh, Ashraf Mohamadkhani, Saiedeh Erfania. Kaempferol increases apoptosis in human cervical cancer HeLa cells via PI3K/AKT and telomerase pathways. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie. vol 89. 2018-02-14. PMID:28258039. |
cell viability was determined by mtt assay and real time pcr was used to investigate apoptosis and telomerase genes expression. |
2018-02-14 |
2023-08-13 |
human |
| Wen-Ji Shangguan, Yue-Hui Zhang, Zhan-Chun Li, Lu-Min Tang, Jiang Shao, He L. Naringin inhibits vascular endothelial cell apoptosis via endoplasmic reticulum stress‑ and mitochondrial‑mediated pathways and promotes intraosseous angiogenesis in ovariectomized rats. International journal of molecular medicine. vol 40. issue 6. 2018-02-14. PMID:29039439. |
mtt assay was used to detect cell proliferation while hoechst 33258 staining and flow cytometry were used to detect apoptosis. |
2018-02-14 |
2023-08-13 |
rat |
| Chunling Jiang, Tao Song, Jingao Li, Fan Ao, Xiaochang Gong, Yicheng Lu, Chenran Zhang, Liangyu Chen, Yunhui Liu, Hua He, Ouping Huan. RAS Promotes Proliferation and Resistances to Apoptosis in Meningioma. Molecular neurobiology. vol 54. issue 1. 2018-02-08. PMID:27021020. |
methyl-thiazole-tetrazolium bromide (mtt) assay and flow cytometer (with cells after fts (75 μmol/l) treatment for 48 h) were utilized to determine the proliferation and apoptosis, respectively, of iomm-lee cells after ras inhibition. |
2018-02-08 |
2023-08-13 |
mouse |
| Gilda Kalinec, Pru Thein, Channy Park, Federico Kaline. HEI-OC1 cells as a model for investigating drug cytotoxicity. Hearing research. vol 335. 2018-02-05. PMID:26930622. |
we evaluated cell toxicity, apoptosis, viability, proliferation, senescence and autophagy in response to apap (acetaminophen), cisplatin, dexamethasone, gentamicin, penicillin, neomycin, streptomycin, and tobramycin, at five different doses and two time-points (24 and 48 h), by flow cytometry techniques and caspase 3/7, mtt, cytotoxicity, brdu, beclin1, lc3 and sa-β-galactosidase assays. |
2018-02-05 |
2023-08-13 |
mouse |
| Ayman A M Alameen, Carolina Simioni, Alberto M Martelli, Giorgio Zauli, Simona Ultimo, James A McCubrey, Arianna Gonelli, Giorgia Marisi, Paola Ulivi, Silvano Capitani, Luca M Ner. Healthy CD4+ T lymphocytes are not affected by targeted therapies against the PI3K/Akt/mTOR pathway in T-cell acute lymphoblastic leukemia. Oncotarget. vol 7. issue 34. 2018-02-05. PMID:27494886. |
inhibitor effects on cell cycle and apoptosis were analyzed by flow cytometry, while cytotoxicity was assessed by mtt assays. |
2018-02-05 |
2023-08-13 |
human |
| Xiuyuan He, Jing Wu, Liyun Yuan, Feng Lin, Jine Yi, Jing Li, Hui Yuan, Jinling Shi, Tingting Yuan, Shufang Zhang, Yongheng Fan, Zhihang Zha. Lead induces apoptosis in mouse TM3 Leydig cells through the Fas/FasL death receptor pathway. Environmental toxicology and pharmacology. vol 56. 2018-01-31. PMID:28889079. |
the effects of pb on cell proliferation and apoptosis were analyzed with mtt and annexin v-fitc/pi via flow cytometry, respectively. |
2018-01-31 |
2023-08-13 |
mouse |
| Chen Liu, Chaoyu Mu, Zeng Li, Liang X. Imazamethabenz inhibits human breast cancer cell proliferation, migration and invasion via combination with Pin1. Molecular medicine reports. vol 15. issue 5. 2018-01-22. PMID:28350086. |
mtt assays, western blotting, wound healing assay and matrigel invasion assays revealed that imazamethabenz induced apoptosis and inhibited migration and invasion of the breast cancer cell line mcf‑7, which overexpresses pin1, by inhibiting the pin1‑mediated signaling pathway involving vascular endothelial growth factor and matrix metalloproteinase 9. |
2018-01-22 |
2023-08-13 |
human |
| Liping Liu, Yonghua Yuan, Xuehua He, Xiaohui Xia, Xiaoyang M. MicroRNA-1 upregulation promotes myocardiocyte proliferation and suppresses apoptosis during heart development. Molecular medicine reports. vol 15. issue 5. 2018-01-19. PMID:28260051. |
the effect of mir‑1 overexpression on the viability and apoptosis of differentiated p19 cells was assessed using mtt and annexin v‑fitc assays, respectively. |
2018-01-19 |
2023-08-13 |
Not clear |
| R Nedaeinia, M Sharifi, A Avan, M Kazemi, L Rafiee, M Ghayour-Mobarhan, R Saleh. Locked nucleic acid anti-miR-21 inhibits cell growth and invasive behaviors of a colorectal adenocarcinoma cell line: LNA-anti-miR as a novel approach. Cancer gene therapy. vol 23. issue 8. 2018-01-12. PMID:27364574. |
the viability of the cells was evaluated by mtt (3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyl tetrazolium bromide) assay and annexin v/propidium iodide staining assay was used to detect apoptosis. |
2018-01-12 |
2023-08-13 |
human |
| Panshi Yan, Shu Tang, Haifeng Zhang, Yuanyuan Guo, Zhiwen Zeng, Qiang We. Palmitic acid triggers cell apoptosis in RGC-5 retinal ganglion cells through the Akt/FoxO1 signaling pathway. Metabolic brain disease. vol 32. issue 2. 2018-01-12. PMID:27928692. |
we found that pa exposure significantly decreased cell viability by enhancing apoptosis in rgc-5 cells, as measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (mtt) assay and flow cytometry. |
2018-01-12 |
2023-08-13 |
rat |
| Lei Yan, Yan Wang, Jun Liang, Zhixin Liu, Xiaodong Sun, Kerui Ca. MiR-301b promotes the proliferation, mobility, and epithelial-to-mesenchymal transition of bladder cancer cells by targeting EGR1. Biochemistry and cell biology = Biochimie et biologie cellulaire. vol 95. issue 5. 2018-01-12. PMID:28521108. |
cell proliferation, cell cycle, and apoptosis were analyzed by mtt assay, colony-forming assay, and flow cytometry, respectively. |
2018-01-12 |
2023-08-13 |
human |
| Lei Yan, Kerui Cai, Jun Liang, Haifeng Liu, Yang Liu, Jinqiu Gu. Interaction between miR-572 and PPP2R2C, and their effects on the proliferation, migration, and invasion of nasopharyngeal carcinoma (NPC) cells. Biochemistry and cell biology = Biochimie et biologie cellulaire. vol 95. issue 5. 2018-01-12. PMID:28525724. |
mtt assay and flow cytometry were applied to investigate the viability and apoptosis levels of npc cells. |
2018-01-12 |
2023-08-13 |
Not clear |
| Buhai Wang, Yizhi Ge, Xiang G. Analysis of esophageal cancer cell lines exposed to X-ray based on radiosensitivity influence by tumor necrosis factor-α. Journal of X-ray science and technology. vol 24. issue 5. 2018-01-11. PMID:27127936. |
mtt assay was used to evaluate the cell growth curve, and flow cytometry was performed to assess the cell apoptosis. |
2018-01-11 |
2023-08-13 |
Not clear |