| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Hairuo Lin, Liang Shen, Xiajun Zhang, Jiahe Xie, Huixin Hao, Yingxue Zhang, Zhenhuan Chen, Hiroshi Yamamoto, Wangjun Liao, Jianping Bin, Shiping Cao, Xiaobo Huang, Yulin Lia. HMGB1-RAGE Axis Makes No Contribution to Cardiac Remodeling Induced by Pressure-Overload. PloS one. vol 11. issue 6. 2017-07-19. PMID:27355349. |
cultured neonatal rat cardiomyocytes were treated with different concentrations of recombinant hmgb1, then cell viability was determined using mtt and cck8 assays and cell apoptosis was determined by hoechst staining and tunel assay. |
2017-07-19 |
2023-08-13 |
mouse |
| Hongxin Chen, Zhao Zhong Chong, Sonia M De Toledo, Edouard I Azzam, Stella Elkabes, Nizar Souaya. Delayed activation of human microglial cells by high dose ionizing radiation. Brain research. vol 1646. 2017-07-18. PMID:27265419. |
by one week after irradiation, 20-30% of the cells exposed to high doses of γ rays underwent apoptosis, which correlated with significant concomitant decrease in metabolic activity as assessed by the mtt assay, and microglial activation as judged by both morphological changes and increased expression of glut-5 and cr43. |
2017-07-18 |
2023-08-13 |
human |
| Yun Luan, Junli Liu, Xiaoli Liu, Xia Xue, Feng Kong, Chao Sun, Jue Wang, Ling Liu, Hongying Ji. Tetramethypyrazine inhibits renal cell carcinoma cells through inhibition of NKG2D signaling pathways. International journal of oncology. vol 49. issue 4. 2017-07-13. PMID:27633040. |
we showed that tmp significantly inhibited ccrcc cell viability, proliferation, apoptosis, invasion and migration through the methods of mtt, flow cytometry, wound healing and transwell assays. |
2017-07-13 |
2023-08-13 |
human |
| Yiran Yin, Lian Tang, Lei Sh. The metastasis suppressor gene KISS-1 regulates osteosarcoma apoptosis and autophagy processes. Molecular medicine reports. vol 15. issue 3. 2017-07-13. PMID:28075440. |
the mtt method was used to detect apoptosis by flow cytometry, and the mrna levels of apoptosis and autophagy markers caspase-3, bcl-2, bax, lc3 and beclin1 were assessed by rt-pcr. |
2017-07-13 |
2023-08-13 |
Not clear |
| Jai-Sing Yang, Chia-An Lin, Chi-Cheng Lu, Yen-Fang Wen, Fuu-Jen Tsai, Shih-Chang Tsa. Carboxamide analog ITR-284 evokes apoptosis and inhibits migration ability in human lung adenocarcinoma A549 cells. Oncology reports. vol 37. issue 3. 2017-07-13. PMID:28098885. |
we showed that itr-284 inhibited human lung cancer a549 cells using the thiazolyl blue tetrazolium bromide (mtt) assay and evoked apoptosis via the cell cycle distribution at s phase arrest. |
2017-07-13 |
2023-08-13 |
human |
| Feng Liang, Bing Wang, Long Bao, Yin-Sheng Zhao, Shi-Min Zhang, Shu-Qin Zhan. Overexpression of ILK promotes temozolomide resistance in glioma cells. Molecular medicine reports. vol 15. issue 3. 2017-07-13. PMID:28138714. |
cell growth and apoptosis were measured by mtt and hoechst staining, and flow cytometry, respectively. |
2017-07-13 |
2023-08-13 |
human |
| Junqing Gao, Tao Chen, Deqiang Zhao, Jianpu Zheng, Zongjun Li. Ginkgolide B Exerts Cardioprotective Properties against Doxorubicin-Induced Cardiotoxicity by Regulating Reactive Oxygen Species, Akt and Calcium Signaling Pathways In Vitro and In Vivo. PloS one. vol 11. issue 12. 2017-07-11. PMID:27973574. |
cell viability and cell apoptosis were assessed by mtt assay and hoechst staining, respectively. |
2017-07-11 |
2023-08-13 |
mouse |
| Hong-Zhou Meng, Xiao-Feng Ni, Hai-Ning Yu, Shan-Shan Wang, Sheng-Rong She. Effects of astaxanthin on oxidative stress induced by Cu Journal of Zhejiang University. Science. B. vol 18. issue 2. 2017-07-11. PMID:28124844. |
in the current paper, the effects of ast on viability of prostate cells were investigated by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (mtt) assay; cell apoptosis and intracellular reactive oxygen species (ros) levels were determined by flow cytometry; the mitochondrial membrane potential (mmp) was measured by fluorospectrophotometer; and activities of malondialdehyde (mda), superoxide dismutase (sod), catalase (cat), and glutathione peroxidase (gsh-px) were evaluated by a detection kit. |
2017-07-11 |
2023-08-13 |
cat |
| Zhiqiang Li, Yang Sun, Hongxing Wan, Fang Cha. [Overexpression of N-myc downstream regulated gene 2 (NDRG2) inhibits proliferation, migration and promotes apoptosis in SW480 rectal cancer cells]. Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology. vol 33. issue 1. 2017-07-10. PMID:28031114. |
cell proliferation was detected in sw480 cells, sw480-ve cells and sw480-ndrg2 cells by mtt assay; cell migration distance in the three groups at 24, 48, 72 hours was tested by wound healing assay; apoptosis rate was determined in the three groups at 48 hours by flow cytometry; the expressions of bax, caspase-3, bcl-2 proteins in the three groups were examined by western blotting. |
2017-07-10 |
2023-08-13 |
human |
| Xia Yang, Rui Wang, Kai Xu, Fan Yang, Yanbin Chen, Zhi Zuo, Bin Son. [Knockdown of myeloid cell leukemia 1 gene inhibits the proliferation and promotes apoptosis of prostate cancer cells]. Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology. vol 33. issue 1. 2017-07-10. PMID:28031117. |
in addition, after the expression of human mcl-1 protein in prostate cancer cell line was interfered, mtt assay, clone formation assay and tunel assay were performed to test the ability of cell proliferation and apoptosis. |
2017-07-10 |
2023-08-13 |
human |
| Yiying Wu, Xing Zhang, Zhengzhao Li, Haibiao Yan, Jian Qin, Tianyu L. Formononetin inhibits human bladder cancer cell proliferation and invasiveness via regulation of miR-21 and PTEN. Food & function. vol 8. issue 3. 2017-07-10. PMID:28139790. |
t24 cells were treated with different concentrations of formononetin, and then the cell proliferation was assessed by mtt assay, cell apoptosis by hoechst 33258 stain assay, cell invasiveness by transwell invasion assay, microrna-21 (mir-21) expression by real-time pcr and the protein level of phosphatase and tensin homolog (pten) and phosphorylated homolog of akt (p-akt) by western blotting. |
2017-07-10 |
2023-08-13 |
human |
| Yuan Mao, Jun Wang, Mingzhi Zhang, Weifei Fan, Qi Tang, Siping Xiong, Xiaojun Tang, Juqing Xu, Lin Wang, Shu Yang, Suyao Liu, Li Xu, Yan Chen, Lin Xu, Rong Yin, Jin Zh. A neutralized human LMP1-IgG inhibits ENKTL growth by suppressing the JAK3/STAT3 signaling pathway. Oncotarget. vol 8. issue 7. 2017-07-07. PMID:28009988. |
cck-8, mtt, apoptosis assays, antibody-dependent cell-mediated cytotoxicity (adcc) and cdc (complement-dependent cytotoxicity) assays were performed to evaluate the inhibitory effects of lmp1-igg on extranodal nasal-type natural killer (nk)/t-cell lymphoma (enktl). |
2017-07-07 |
2023-08-13 |
human |
| Hongying Liu, Gang Wang, Lili Yang, Jianjun Qu, Zhihui Yang, Xiangyu Zho. Knockdown of Long Non-Coding RNA UCA1 Increases the Tamoxifen Sensitivity of Breast Cancer Cells through Inhibition of Wnt/β-Catenin Pathway. PloS one. vol 11. issue 12. 2017-07-05. PMID:27977766. |
we tested the viability, invasive ability and apoptosis rate in mcf-7 and t47d cells using mtt assay, transwell assay and flow cytometry assay, respectively. |
2017-07-05 |
2023-08-13 |
mouse |
| Gamze Altıntaş Kazar, Ece Şe. [Investigation of antitumorigenic effects of food-borne non-pathogenic and pathogenic Salmonella enterica strains on MEF, DU145 and HeLa cell lines]. Mikrobiyoloji bulteni. vol 50. issue 3. 2017-06-30. PMID:27525394. |
the cell viability was measured by colorimetric mtt cytotoxicity assay, the percentage of apoptosis was assessed by tali® apoptosis assay-annexin v alexa fluor® 488 kit (invitrogen, molecular probes, life technologies, usa), and the caspase-3 activity was determined by colorimetric protease apotarget™ kit (invitrogen, biosource international, usa). |
2017-06-30 |
2023-08-13 |
mouse |
| Jia-Zhi Wang, Bi-Sheng Huang, Yan Cao, Ke-Li Chen, Juan L. Anti-hepatoma activities of ethyl acetate extract from Ampelopsis sinica root. Oncology reports. vol 37. issue 4. 2017-06-29. PMID:28350090. |
this study was designed to investigate cytotoxicity by mtt assay, induction of apoptosis via hoechst 33258 staining, scanning electron microscopy and bivariate flow cytometric analysis (annexin v-fitc/pi), inflammation and apoptosis related genes expression by rt-pcr and p53 protein expression by immunofluorescence assay in hepg2 cells. |
2017-06-29 |
2023-08-13 |
mouse |
| Linghong Wu, Yuan Zhang, Ying Zhu, Qingwei Cong, Yan Xiang, Linlin F. The effect of LOXL2 in hepatocellular carcinoma. Molecular medicine reports. vol 14. issue 3. 2017-06-27. PMID:27430160. |
the present study constructed loxl2‑small interfering rna with a lentiviral vector, investigated the effect of loxl2 on proliferation using hcc cell lines via a series of assays, including reverse transcription‑quantitative polymerase chain reaction, cell counting, colony formation, assessment of cell cycle and apoptosis using flow cytometry, mtt and brdu. |
2017-06-27 |
2023-08-13 |
Not clear |
| Famin Ye, Rongqing Sun, Qingmin Li, Jingping Lian. Influence of hemodynamics on myocardial cell cardiac index and its molecular mechanism. Pakistan journal of pharmaceutical sciences. vol 29. issue 5 Suppl. 2017-06-26. PMID:28476698. |
and the mtt was adopted to detect huvec growth and the flow cytometry (flow cytometry, fct) to detect huvec apoptosis. |
2017-06-26 |
2023-08-13 |
human |
| Lijie Song, Weijie Zhang, Zhiwei Chang, Yanfeng Pan, Hong Zong, Qingxia Fan, Liuxing Wan. miR-4417 Targets Tripartite Motif-Containing 35 (TRIM35) and Regulates Pyruvate Kinase Muscle 2 (PKM2) Phosphorylation to Promote Proliferation and Suppress Apoptosis in Hepatocellular Carcinoma Cells. Medical science monitor : international medical journal of experimental and clinical research. vol 23. 2017-06-22. PMID:28394882. |
cell proliferation was measured by mtt assay and apoptosis in a549 cells was examined by flow cytometry. |
2017-06-22 |
2023-08-13 |
Not clear |
| Zhankun Zhu, Jinhua Dai, Yufeng Liao, Tao Wan. Sox9 Protects against Human Lung Fibroblast Cell Apoptosis Induced by LPS through Activation of the AKT/GSK3β Pathway. Biochemistry. Biokhimiia. vol 82. issue 5. 2017-06-21. PMID:28601070. |
then 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2h-tetrazolium bromide (mtt) assay and flow cytometry were used to explore, respectively, proliferation and apoptosis in vitro. |
2017-06-21 |
2023-08-13 |
human |
| Lele Lu, Linlin Chai, Wenping Wang, Xi Yuan, Shirong Li, Chuan Ca. A Selenium-Enriched Ziyang Green Tea Polysaccharide Induces Bax-Dependent Mitochondrial Apoptosis and Inhibits TGF-β1-Stimulated Collagen Expression in Human Keloid Fibroblasts via NG2 Inactivation. Biological trace element research. vol 176. issue 2. 2017-06-19. PMID:27565798. |
3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyltetrazolium bromide (mtt) and annexin v/propidium iodide (pi) staining assays demonstrated that se-zgtp-i or neuron-glia 2 (ng2) short hairpin rna (shrna) significantly inhibited proliferation of human keloid fibroblasts via induction of apoptosis. |
2017-06-19 |
2023-08-13 |
human |