| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Yuqiang Wang, Xiaohua Wang, Xuetao Su, Tiansheng Li. HIF-2α affects proliferation and apoptosis of MG-63 osteosarcoma cells through MAPK signaling. Molecular medicine reports. vol 15. issue 4. 2017-05-15. PMID:28259908. |
cell viability of mg‑63 in the silenced and the control groups was determined by mtt assay; cell apoptosis was measured by flow cytometry; the expression of hif‑2α and mitogen-activated protein kinase (mapk)-p38 were measured by western blotting. |
2017-05-15 |
2023-08-13 |
Not clear |
| Jia Huang, Lan Xiao, Jing-Xiang Wei, Ya-Hai Shu, Shi-Qi Fang, Yong-Tang Wang, Xiu-Min L. Protective effect of arctigenin on ethanol-induced neurotoxicity in PC12 cells. Molecular medicine reports. vol 15. issue 4. 2017-05-15. PMID:28260103. |
to address this issue, the present study used rat pheochromocytoma pc12 cells to investigate the neuroprotective effects of atg on ethanol-induced cell damage by performing an mtt reduction assay, cell cycle analysis, hoechst33342/propidium iodide fluorescence staining and flow cytometry to examine apoptosis. |
2017-05-15 |
2023-08-13 |
mouse |
| Lu Jin, Xi Li, Yifan Li, Zeng Zhang, Tao He, Jia Hu, Jiaju Liu, Mingwei Chen, Min Shi, Zhimao Jiang, Yaoting Gui, Shangqi Yang, Xiangming Mao, Yongqing La. Identification of miR‑195‑3p as an oncogene in RCC. Molecular medicine reports. vol 15. issue 4. 2017-05-12. PMID:28260025. |
to assess the function of mir‑195‑3p in rcc cell lines, cell proliferation was examined using mtt and cck‑8 assays, mobility was assessed using a cell scratch assay, transwell migration assay and invasion assay, and apoptosis was examined using flow cytometry. |
2017-05-12 |
2023-08-13 |
Not clear |
| Di Wu, Weiguo Shi, Jing Zhao, Zhengren Wei, Zhijia Chen, Dawei Zhao, Shijie Lan, Jiandong Tai, Bohua Zhong, Hong Y. Assessment of the chemotherapeutic potential of a new camptothecin derivative, ZBH-1205. Archives of biochemistry and biophysics. vol 604. 2017-05-09. PMID:27302903. |
comparisons between the different compounds were made on the basis of ic50 values as determined by the mtt assay, and flow cytometry was used to evaluate cell cycle progression, apoptosis, and the levels of pro- and active caspase-3 among different treatment groups. |
2017-05-09 |
2023-08-13 |
human |
| Feng Liu, Zhuang-Zhuang Huang, Yu-Hong Sun, Ting Li, Dong-Hua Yang, Gang Xu, Ying-Ying Su, Tao Zhan. Four Main Active Ingredients Derived from a Traditional Chinese Medicine Guanxin Shutong Capsule Cause Cardioprotection during Myocardial Ischemia Injury Calcium Overload Suppression. Phytotherapy research : PTR. vol 31. issue 3. 2017-05-09. PMID:28164397. |
in vitro, rat neonatal cardiomyocytes were isolated, the cell viability and apoptosis rate were, respectively, measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (mtt) method and fluorescence activating cell sorter, and the intracellular calcium concentration ([ca |
2017-05-09 |
2023-08-13 |
rat |
| Jin-Guo Li, Qi Hao, Ying-Xue Liu, Peng Li, Peng Li, Xia-Yan Shao, Hua Tian, Yong-Qi Fang, Shu-Tong Ya. [Advanced glycated albumin induces macrophage apoptosis via activating caspase-12 pathway]. Sheng li xue bao : [Acta physiologica Sinica]. vol 68. issue 6. 2017-05-05. PMID:28004067. |
after incubation for 24 h, the cell viability and apoptosis were determined by using mtt assay and tunel detection kit, respectively. |
2017-05-05 |
2023-08-13 |
Not clear |
| S J Peng, J Li, Y Zhou, M Tuo, X X Qin, Q Yu, H Cheng, Y M L. In vitro effects and mechanisms of lycopene in MCF-7 human breast cancer cells. Genetics and molecular research : GMR. vol 16. issue 2. 2017-05-04. PMID:28407181. |
in addition, reduced cell proliferation and increased apoptosis (p < 0.05) were observed using mtt assay and flow cytometry, respectively. |
2017-05-04 |
2023-08-13 |
human |
| Chen-Bin Yu, Guo-Long Zhao, Li-Ming Yu, Shi-Qiang Yu, Wei-Xun Duan, Hai-Feng Zhan. [Proanthocyanidin protects H9C2 cells against hypoxia/reoxygenation injury via JAK2/STAT3 signaling pathway]. Sheng li xue bao : [Acta physiologica Sinica]. vol 68. issue 5. 2017-05-03. PMID:27778021. |
cellular viability and apoptosis rate were detected by mtt and tunel methods, and superoxide generation was measured. |
2017-05-03 |
2023-08-13 |
Not clear |
| Noelia Nikoloff, Ana M Pascua, Juan M Anchordoquy, Juan P Anchordoquy, Matías A Sirini, Analia Seoane, Cecilia C Furnu. Effect of eicosapentaenoic acid on bovine cumulus-oocyte complex in vitro. Cell biology international. vol 41. issue 5. 2017-05-01. PMID:28198569. |
the effect of epa was evaluated on cumulus-oocyte complexes (coc) by oocyte maturation (cumulus expansion area and oocyte nuclear maturation), genotoxicity [single cell gel electrophoresis (scge)], and cytotoxicity (apoptosis, viability, and mtt assays) end points. |
2017-05-01 |
2023-08-13 |
cattle |
| Wen-Bin Liu, Fei Xie, Hui-Qing Sun, Meng Meng, Zhen-Yuan Zh. Anti-tumor effect of polysaccharide from Hirsutella sinensis on human non-small cell lung cancer and nude mice through intrinsic mitochondrial pathway. International journal of biological macromolecules. vol 99. 2017-04-24. PMID:28235606. |
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (mtt) assay showed that hsp-iii induces the apoptosis of h1299 cells; however the proliferation viability of normal lung epithelial cells is not affected. |
2017-04-24 |
2023-08-13 |
mouse |
| Qian Wang, Chao Yan, Miaomiao Xin, Li Han, Yunqing Zhang, Mingshu Su. Sirtuin 1 (Sirt1) Overexpression in BaF3 Cells Contributes to Cell Proliferation Promotion, Apoptosis Resistance and Pro-Inflammatory Cytokine Production. Medical science monitor : international medical journal of experimental and clinical research. vol 23. 2017-04-24. PMID:28346398. |
then the transfected cells viability and apoptosis were respectively determined by mtt assay and flow cytometry. |
2017-04-24 |
2023-08-13 |
mouse |
| Chengyi Huang, Yufeng Y. Synergistic Cytotoxicity of β-Elemene and Cisplatin in Gingival Squamous Cell Carcinoma by Inhibition of STAT3 Signaling Pathway. Medical science monitor : international medical journal of experimental and clinical research. vol 23. 2017-04-24. PMID:28355175. |
mtt assay, clonogenic assay, annexin v/pi apoptosis assay, western blot analysis, and xenograft model treatment were carried out in vitro and in vivo. |
2017-04-24 |
2023-08-13 |
Not clear |
| Ying-Ying Shao, Tao-Lan Zhang, Lan-Xiang Wu, He-Cun Zou, Shuang Li, Jin Huang, Hong-Hao Zho. AKT Axis, miR-21, and RECK Play Pivotal Roles in Dihydroartemisinin Killing Malignant Glioma Cells. International journal of molecular sciences. vol 18. issue 2. 2017-04-20. PMID:28208619. |
in this study, methyl thiazolyl tetrazolium (mtt), colony-forming, wound healing, invasion, and apoptosis assays were performed to investigate the effect of dha on malignant glioma cells. |
2017-04-20 |
2023-08-13 |
Not clear |
| Haiyan Ding, Rong Han, Xueshan Chen, Weirong Fang, Meng Liu, Xuemei Wang, Qin Wei, Nandani Darshika Kodithuwakku, Yunman L. Clematichinenoside (AR) Attenuates Hypoxia/Reoxygenation-Induced H9c2 Cardiomyocyte Apoptosis via a Mitochondria-Mediated Signaling Pathway. Molecules (Basel, Switzerland). vol 21. issue 6. 2017-04-18. PMID:27248986. |
the viability of h9c2 cardiomyocytes was determined by mtt assay; apoptosis was evaluated by flow cytometry and tunel experiments; mitochondrial permeability transition pore (mptp) opening was analyzed by a calcein-cobalt quenching method; and mitochondrial membrane potential (ΔΨm) was detected by jc-1. |
2017-04-18 |
2023-08-13 |
Not clear |
| Shi Zhong, You-Gui Li, Dong-Feng Ji, Tian-Bao Lin, Zhi-Qiang L. Protocatechualdehyde Induces S-Phase Arrest and Apoptosis by Stimulating the p27(KIP1)-Cyclin A/D1-CDK2 and Mitochondrial Apoptotic Pathways in HT-29 Cells. Molecules (Basel, Switzerland). vol 21. issue 7. 2017-04-18. PMID:27447597. |
we performed an in vitro study involving mtt, flow cytometry, rt-pcr, and western blot analyses to investigate the effects of pca treatment on cell proliferation, cell cycle distribution, apoptosis, and expression of several cell cycle-related genes in ht-29 cells. |
2017-04-18 |
2023-08-13 |
human |
| Kai Wu, Longan Ma, Jinxiang Zh. miR‑483‑5p promotes growth, invasion and self‑renewal of gastric cancer stem cells by Wnt/β‑catenin signaling. Molecular medicine reports. vol 14. issue 4. 2017-04-17. PMID:27511210. |
an mtt assay demonstrated that overexpression of mir‑483‑5p by transfection with mir‑483‑5p mimics significantly increased cell proliferation and annexin v‑propidium iodide staining indicated the suppression of cell apoptosis, suggesting that mir‑483‑5p has an important function in gcsc growth. |
2017-04-17 |
2023-08-13 |
Not clear |
| Lin Yang, Yanqing Liu, Mei Wang, Yayun Qian, Xiaoyun Dong, Hao Gu, Haibo Wang, Shiyu Guo, Tadashi Hisamits. Quercetin-induced apoptosis of HT-29 colon cancer cells via inhibition of the Akt-CSN6-Myc signaling axis. Molecular medicine reports. vol 14. issue 5. 2017-04-17. PMID:27748879. |
the present study investigated the effects of quercetin on ht-29 human colorectal cancer cell viability, apoptosis and cell cycle arrest using an mtt assay, flow cytometry, transmission electron microscopy and western blotting. |
2017-04-17 |
2023-08-13 |
human |
| Feng-Xiao Ge, Haitao Li, Xin Yi. Upregulation of microRNA-125b-5p is involved in the pathogenesis of osteoarthritis by downregulating SYVN1. Oncology reports. vol 37. issue 4. 2017-04-17. PMID:28260078. |
finally, mtt assay was employed to estimate the effect of mir-125b-5p on apoptosis. |
2017-04-17 |
2023-08-13 |
Not clear |
| Rebecca Klippstein, Sukhvinder S Bansal, Khuloud T Al-Jama. Doxorubicin enhances curcumin's cytotoxicity in human prostate cancer cells in vitro by enhancing its cellular uptake. International journal of pharmaceutics. vol 514. issue 1. 2017-04-14. PMID:27863661. |
cell size and complexity, cytotoxicity and apoptosis were studied by flow cytometry, mtt assay and sub-g1 quantification, respectively. |
2017-04-14 |
2023-08-13 |
human |
| Longwei Hu, Jing Han, Xi Yang, Yang Wang, Hongya Pan, Liqun X. Apoptosis repressor with caspase recruitment domain enhances survival and promotes osteogenic differentiation of human osteoblast cells under Zoledronate treatment. Molecular medicine reports. vol 14. issue 4. 2017-04-13. PMID:27573706. |
cell numbers were detected using the mtt assay, and flow cytometry was used to identity cell apoptosis. |
2017-04-13 |
2023-08-13 |
mouse |