| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Yingliang Ou, Jiafa He, Yubin Li. MiR-490-3p inhibits autophagy via targeting ATG7 in hepatocellular carcinoma. IUBMB life. vol 70. issue 6. 2019-05-28. PMID:29676845. |
the cell proliferation was tested via colony formation assay and cck-8 after the mir-490-3p mimics were transfected into hepg2 cells; the variations of cell cycle and apoptosis was examined by flow cytometry assay; the number of autophagosome was observed by electron microscopy and the changes of autophagy-relative protein lc-ii and lc-i as well as their ratio was tested by western blot. |
2019-05-28 |
2023-08-13 |
Not clear |
| Mustafa Caglar Beker, Berrak Caglayan, Esra Yalcin, Ahmet Burak Caglayan, Seyma Turkseven, Busra Gurel, Taha Kelestemur, Elif Sertel, Zafer Sahin, Selim Kutlu, Ulkan Kilic, Ahmet Tarik Baykal, Ertugrul Kili. Time-of-Day Dependent Neuronal Injury After Ischemic Stroke: Implication of Circadian Clock Transcriptional Factor Bmal1 and Survival Kinase AKT. Molecular neurobiology. vol 55. issue 3. 2019-05-23. PMID:28421530. |
besides infarct volume and brain swelling, neuronal survival, apoptosis, ischemia, and circadian rhythm related proteins were examined using immunohistochemistry, western blot, planar surface immune assay, and liquid chromatography-mass spectrometry tools. |
2019-05-23 |
2023-08-13 |
mouse |
| Sang Hee Park, Dong Sam Kim, Sunggyu Kim, Laura Rojas Lorz, Eunju Choi, Hye Yeon Lim, Mohammad Amjad Hossain, SeokGu Jang, Young Im Choi, Kyung Ja Park, Keejung Yoon, Jong-Hoon Kim, Jae Youl Ch. Loliolide Presents Antiapoptosis and Antiscratching Effects in Human Keratinocytes. International journal of molecular sciences. vol 20. issue 3. 2019-05-21. PMID:30717391. |
the apoptosis signaling pathway was confirmed by western blot analysis, which showed that loliolide was able to reduce the expression of caspases 3, 8, and 9, which are related to ros-induced apoptosis. |
2019-05-21 |
2023-08-13 |
human |
| Yi Zhang, Li Zhang, Qiyu Zhang, Weilong Hong, Xiaohua Li. [microRNA-222 regulates proliferation and apoptosis of fibroblasts in hypertrophic scar via matrix metalloproteinase 1]. Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences. vol 46. issue 6. 2019-05-20. PMID:29658663. |
the cell viability was tested with mtt assay, cell cycle distribution and apoptosis were detected with flow cytometry and the expression levels of proliferation, apoptosis and cell cycle related proteins were determined with western blot. |
2019-05-20 |
2023-08-13 |
Not clear |
| Pei Chen, Hui-Ping Liu, Hai-Hu Ji, Na-Xin Sun, Ying-Ying Fen. A cold-water soluble polysaccharide isolated from Grifola frondosa induces the apoptosis of HepG2 cells through mitochondrial passway. International journal of biological macromolecules. vol 125. 2019-05-20. PMID:30236758. |
and western blot analysis of bax, bcl-2, cytochrome c (cyto-c), caspase-3, and caspase-9 further demonstrated that the cgfp-induced apoptosis effect functioned through the mitochondrial pathway. |
2019-05-20 |
2023-08-13 |
human |
| Min Zhou, Weibing L. Ent-Dihydrotucumanoic acid promotes apoptosis in PC-3 human prostate cancer cells. Cellular and molecular biology (Noisy-le-Grand, France). vol 65. issue 3. 2019-05-20. PMID:30942164. |
the effect of dta on the growth of tumor cell line was studied by cck-8 method, mitochondrial membrane potential and apoptosis were detected by flow cytometry, apoptosis-related genes were detected by western blot assay, and the absorptivity of caspase-3 and caspase-9 was measured by spectrophotometer. |
2019-05-20 |
2023-08-13 |
human |
| Tianqi Liu, Tianshu Yang, Zhe Xu, Siran Tan, Tingru Pan, Na Wan, Shu L. MicroRNA-193b-3p regulates hepatocyte apoptosis in selenium-deficient broilers by targeting MAML1. Journal of inorganic biochemistry. vol 186. 2019-05-16. PMID:29990747. |
we tested the apoptosis of se-deficient broiler livers and microrna-193b-transfected primary hepatocytes using qpcr, western blot (wb) and flow cytometry. |
2019-05-16 |
2023-08-13 |
Not clear |
| Jing Xu, Jian He, Huang He, Renjun Peng, Jian X. TWEAK-Fn14 Influences Neurogenesis Status via Modulating NF-κB in Mice with Spinal Cord Injury. Molecular neurobiology. vol 54. issue 9. 2019-05-08. PMID:27822714. |
apoptosis status in the injured area was examined via tdt-mediated dutp-biotin nick end-labeling (tunel) staining, the expression of gap-43 in injured spinal cord was quantified by immunohistochemistry and expressions of tweak, fn14, nf-κb, tnf-α, and il-1β were evaluated by either western blot or elisa. |
2019-05-08 |
2023-08-13 |
mouse |
| Ya-Nan Song, Ding Yuan, Chang-Cheng Zhang, Luo-Pei Wang, Yu-Min He, Ting Wang, Zhi-Yong Zho. [Effect of saponins extracted from Panax japonicus on inhibiting cardiomyocyte apoptosis by AMPK/Sirt1/NF-κB signaling pathway in aging rats]. Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica. vol 42. issue 23. 2019-05-08. PMID:29376267. |
their myocardial morphology changes were observed by using haematoxylin-eoin(he) staining; cardiomyocyte apoptosis was tested by using tunel assays; and the protein expression levels of bcl-2, bax, il-1β, tnf-α, ampk, p-ampk, sirt1, and ac-nf-κb p65 in myocardial tissues of rats were detected by western blot. |
2019-05-08 |
2023-08-13 |
rat |
| Haibin Yan, Bingyun Zhang, Chongbin Fang, Liqiu Che. miR-340 alleviates chemoresistance of osteosarcoma cells by targeting ZEB1. Anti-cancer drugs. vol 29. issue 5. 2019-05-08. PMID:29494357. |
plasmid construction and transfection, mirna arrays, pcr analyses, and western blot analysis, as well as mtt, apoptosis, and luciferase assays were carried out in mg-63 cells and mg-63/cisplatin (ddp)-resistant cells. |
2019-05-08 |
2023-08-13 |
Not clear |
| Yan Wang, Hui Wang, Hui Ge, Zhuo Yan. AG-1031 induced autophagic cell death and apoptosis in C6 glioma cells associated with Notch-1 signaling pathway. Journal of cellular biochemistry. vol 119. issue 7. 2019-05-08. PMID:29575026. |
results from hoechst 33342 staining and western blot assay indicated that ag-1031 induced c6 cell apoptosis. |
2019-05-08 |
2023-08-13 |
Not clear |
| Rahyza I F Assis, Malgorzata Wiench, Karina G Silvério, Rodrigo A da Silva, Geórgia da Silva Feltran, Enilson A Sallum, Marcio Z Casati, Francisco H Nociti, Denise C Andi. RG108 increases NANOG and OCT4 in bone marrow-derived mesenchymal cells through global changes in DNA modifications and epigenetic activation. PloS one. vol 13. issue 12. 2019-05-06. PMID:30507955. |
hbmscs were treated with complete culture medium, 50 μm rg108 and dmso for three days and subjected to viability and apoptosis assays, global and gene-specific methylation/hydroxymethylation, transcript levels' analysis of epigenetic machinery enzymes and multipotency markers, protein activities of dnmts and tets, immunofluorescence staining and western blot analysis for nanog and oct4 and flow cytometry for cd105. |
2019-05-06 |
2023-08-13 |
human |
| Dong Wang, Liang Tang, Huihui Wu, Kai Wang, Dongyun G. MiR-127-3p inhibits cell growth and invasiveness by targeting ITGA6 in human osteosarcoma. IUBMB life. vol 70. issue 5. 2019-05-03. PMID:29573114. |
the expression of apoptosis and migration related were also detected by western blot. |
2019-05-03 |
2023-08-13 |
human |
| Xiaoxiao Gao, Li Han, Nan Ding, Yu Mu, Peipei Guan, Caijuan Hu, Xueshi Huan. Bafilomycin C1 induces G0/G1 cell-cycle arrest and mitochondrial-mediated apoptosis in human hepatocellular cancer SMMC7721 cells. The Journal of antibiotics. vol 71. issue 9. 2019-05-03. PMID:29752478. |
cell-cycle arrest and induced apoptosis in tumor tissues in a mouse model treated with bafilomycin c1 were demonstrated by histological analyses, western blot and tunel. |
2019-05-03 |
2023-08-13 |
mouse |
| Jindao Wu, Xiongxiong Pan, Heling Fu, Yuan Zheng, Youjin Dai, Yuan Yin, Qin Chen, Qingting Hao, Dan Bao, Daorong Ho. Effect of curcumin on glycerol-induced acute kidney injury in rats. Scientific reports. vol 7. issue 1. 2019-05-01. PMID:28860665. |
finally, cell apoptosis in renal tissue was detected using in situ tunel apoptosis fluorescence staining and expression of proteins associated with apoptotic, oxidative stress and lipid oxidative related signaling pathways were detected using immunohistochemical staining and western blot. |
2019-05-01 |
2023-08-13 |
rat |
| C Wang, Z P Mao, L Wang, F H Zhang, G H Wu, D Y Wang, J L Sh. Cordycepin inhibits cell growth and induces apoptosis in human cholangiocarcinoma. Neoplasma. vol 64. issue 6. 2019-04-29. PMID:28895407. |
in the present study, colony formation assay, cell-counting kit-8 (cck-8) assay and tumor xenograft experiment were performed to evaluate the effect of cordycepin on human cca cell growth in vitro and in vivo; flow cytometric analysis was performed to evaluate the effect of cordycepin on cell apoptosis; quantitative real-time reverse transcription pcr (qrt-pcr) and western blot assays were performed to evaluate the expression levels of caspase-3, bcl-2 and bax. |
2019-04-29 |
2023-08-13 |
human |
| C Wang, Z P Mao, L Wang, F H Zhang, G H Wu, D Y Wang, J L Sh. Cordycepin inhibits cell growth and induces apoptosis in human cholangiocarcinoma. Neoplasma. vol 64. issue 6. 2019-04-29. PMID:28895407. |
furthermore, the flow cytometric analysis, qrt-pcr and western blot assays showed that cordycepin could trigger qbc939 and rbe cells apoptosis by regulating the expression levels of caspase-3, bcl-2 and bax. |
2019-04-29 |
2023-08-13 |
human |
| Wen-Ying Wei, Zhen-Guo Ma, Ning Zhang, Si-Chi Xu, Yu-Pei Yuan, Xiao-Feng Zeng, Qi-Zhu Tan. Overexpression of CTRP3 protects against sepsis-induced myocardial dysfunction in mice. Molecular and cellular endocrinology. vol 476. 2019-04-29. PMID:29655602. |
additionally, ctrp3 suppressed cardiac apoptosis induced by lps in mice as indicated by terminal deoxynucleotidyl transferase nick-end labeling (tunel) staining and western blot for cleaved-caspase3, bax and bcl-2. |
2019-04-29 |
2023-08-13 |
mouse |
| Lulu Liu, Yun Huang, Xiangling Feng, Jihua Chen, Yanying Dua. Overexpressed Hsp70 alleviated formaldehyde-induced apoptosis partly via PI3K/Akt signaling pathway in human bronchial epithelial cells. Environmental toxicology. vol 34. issue 4. 2019-04-22. PMID:30600586. |
apoptosis and the expression levels of pi3k, akt, p-akt, mek, p-mek, and gli2 were detected by annexin-apc/7aad double-labeled flow cytometry and western blot. |
2019-04-22 |
2023-08-13 |
human |
| Yuqian Wang, Hong Zhao, Dezhou Wang, Miao Hao, Chenfei Kong, Xiaoming Zhao, Yiyao Gao, Jing Li, Baocai Liu, Bai Yang, Hao Zhang, Jinlan Jian. Inhibition of Autophagy Promoted Apoptosis and Suppressed Growth of Hepatocellular Carcinoma Upon Photothermal Exposure. Journal of biomedical nanotechnology. vol 15. issue 4. 2019-04-22. PMID:30841973. |
apoptosis was evaluated based on flow cytometry, western blot analysis of apoptosis related proteins (bax, bcl2, caspase 3), hoechst staining, and tunel staining. |
2019-04-22 |
2023-08-13 |
Not clear |