| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Zhilin Xiao, Meifang Chen, Mei Yang, Xiaobin Che. [STAT3 inhibitor Stattic induced IL-8 production and cell apotosis in THP-1 cells by activating ERK pathway]. Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology. vol 35. issue 6. 2019-09-10. PMID:31292053. |
reverse transcription pcr or real-time pcr was performed to detect the mrna expression of il-8, il-6, il-1β and tumor necrosis factor-α (tnf-α); elisa was used to detect the protein expression of il-8; flow cytometry was applied to evaluate the apoptosis of thp-1 cells; and western blot analysis was performed to detect the phosphorylation of stat3 and extracellular signal-regulated kinase (erk). |
2019-09-10 |
2023-08-13 |
Not clear |
| Yabin Hu, Yan Zhou, Gaoxiang Yang, Yalin Wang, Zhongnan Zheng, Juntao Li, Yuting Yan, Wei W. Sulforaphane-N-Acetyl-Cysteine inhibited autophagy leading to apoptosis via Hsp70-mediated microtubule disruption. Cancer letters. vol 431. 2019-09-09. PMID:29807114. |
furthermore, immunofluorescence staining and western blot showed that sfn-nac triggered microtubule disruption causing apoptosis via downregulating α-tubulin and phosphorylated erk1/2-mediated stathmin-1. |
2019-09-09 |
2023-08-13 |
human |
| Xin-Jie Hao, Cheng-Zhen Xu, Jin-Tai Wang, Xiao-Jie Li, Ming-Min Wang, Yi-Hai Gu, Zhi-Gang Lian. miR-21 promotes proliferation and inhibits apoptosis of hepatic stellate cells through targeting PTEN/PI3K/AKT pathway. Journal of receptor and signal transduction research. vol 38. issue 5-6. 2019-09-04. PMID:31038023. |
proliferation, apoptosis, and phosphatase and tensin homolog (pten)/phosphoinositide 3-kinase (pi3k)/protein kinase b (akt) signaling pathway related genes and proteins were detected by quantitative real-time polymerase chain reaction (qrt-pcr) and western blot, respectively. |
2019-09-04 |
2023-08-13 |
Not clear |
| Chunyang Kang, Mingyang Kang, Yingying Han, Tuo Zhang, Wei Quan, Jian Ga. 6-Gingerols (6G) reduces hypoxia-induced PC-12 cells apoptosis and autophagy through regulation of miR-103/BNIP3. Artificial cells, nanomedicine, and biotechnology. vol 47. issue 1. 2019-09-02. PMID:31043087. |
cell viability, apoptosis and its related proteins were studied by the approaches of mtt assay, flow cytometry assay and western blot analysis, respectively. |
2019-09-02 |
2023-08-13 |
rat |
| Lintuo Huang, Zhengxiang Huang, Wenjun Lin, Lu Wang, Xiongbai Zhu, Xin Chen, Shengwu Yang, Chen L. Salidroside suppresses the growth and invasion of human osteosarcoma cell lines MG63 and U2OS in vitro by inhibiting the JAK2/STAT3 signaling pathway. International journal of oncology. vol 54. issue 6. 2019-08-30. PMID:31081055. |
western blot analysis not only confirmed these results but also suggested that salidroside induced the apoptosis of osteosarcoma cells by activating the caspase‑9‑dependent apoptotic pathway. |
2019-08-30 |
2023-08-13 |
human |
| Lintuo Huang, Zhengxiang Huang, Wenjun Lin, Lu Wang, Xiongbai Zhu, Xin Chen, Shengwu Yang, Chen L. Salidroside suppresses the growth and invasion of human osteosarcoma cell lines MG63 and U2OS in vitro by inhibiting the JAK2/STAT3 signaling pathway. International journal of oncology. vol 54. issue 6. 2019-08-30. PMID:31081055. |
furthermore, our findings demonstrated that salidroside induced the apoptosis, g0/g1 phase arrest and suppressed the invasion of osteosarcoma cells by inhibiting the janus kinase 2 (jak2)/signal transducer and activator of transcription 3 (stat3) signaling pathway, as determined by western blot analysis. |
2019-08-30 |
2023-08-13 |
human |
| Yanhua Wang, Bo Li, Xiaofen Zhan. Scutellaria barbata D. Don (SBD) protects oxygen glucose deprivation/reperfusion-induced injuries of PC12 cells by up-regulating Nrf2. Artificial cells, nanomedicine, and biotechnology. vol 47. issue 1. 2019-08-29. PMID:31062620. |
cell viability, apoptosis and proliferation were detected using mtt assay, flow cytometry, ki67 staining and western blot. |
2019-08-29 |
2023-08-13 |
Not clear |
| Bing Xu, Xin He, Yi Sui, Xu Wang, Xia Wang, Li Ren, Yun-Xin Zha. Ginkgetin aglycone attenuates neuroinflammation and neuronal injury in the rats with ischemic stroke by modulating STAT3/JAK2/SIRT1. Folia neuropathologica. vol 57. issue 1. 2019-08-28. PMID:31038184. |
moreover terminal deoxynucleotidyl transferase dutp nick end labelling (tunel) assay was done for determining the neuronal apoptosis and western blot assay also performed for estimating the expression of several proteins. |
2019-08-28 |
2023-08-13 |
rat |
| Yonggen Xu, Xiaolan Wang, Yulv Zhan. Myocardial Infarction-Related Transcripts (MIAT) Participate in Diabetic Optic Nerve Injury by Regulating Heart Shock Protein 5 (HSPA5) via Competitively Binding to MicroRNA-379. Medical science monitor : international medical journal of experimental and clinical research. vol 25. 2019-08-26. PMID:30895947. |
mechanisms underlying the miat-related apoptosis were explored by western blot analysis. |
2019-08-26 |
2023-08-13 |
rat |
| Pei-Bin Lin, Juan Shen, Pei-Yu Ou, Ling-Yan Liu, Zhi-Yu Chen, Fu-Jiang Chu, Jie Wang, Xiao-Bao Ji. Prodigiosin isolated from Serratia marcescens in the Periplaneta americana gut and its apoptosis‑inducing activity in HeLa cells. Oncology reports. vol 41. issue 6. 2019-08-20. PMID:30942457. |
flow cytometry (fcm) was utilized to measure the apoptotic rate with annexin v staining and the expression levels of proteins involved in apoptosis, including b‑cell lymphoma 2 (bcl‑2), bcl‑2‑associated x (bax) and caspase‑3 were determined by western blot analysis and reverse transcription‑quantitative polymerase chain reaction (rt‑qpcr). |
2019-08-20 |
2023-08-13 |
Not clear |
| Kun Peng Song, Peng Shu, Ju Lei Ma, Bing Wang, Bo Che. [Ribosomal Protein S9 Expression in Multiple Myeloma and Its Effect on Cell Biological Function]. Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae. vol 41. issue 2. 2019-08-20. PMID:31060671. |
methods bone marrow mononuclear cells were harvested in 10 healthy volunteers(con group)and bone marrow cd138 +cells from 30 mm patients(cd138+group).quantitative polymerase chain reaction(qpcr)was performed to detect rps9 expression at mrna level.in three cases from con group and 11 cases from cd138+group,western blot was performed to detect rps9 at protein level.gse19784 dataset was employed to detect the relationships of rps9 expression with the overall survival rate,nuclear factor-κb(nf-κb),small ubiquitin-like modifier(sumo),and ubiquitin pathway.after the rps9 knock-down vector was constructed,flow cytometry was performed to detect the infection efficiency and qpcr and western blot to detect the knock-down efficiency.rpmi8226 was divided into con group and rps9-short hairpin rna(shrna)group,in which annexin v allophycocyanin/propidium iodide(pi)double staining was performed to detect the change of apoptosis,cck8 to detect the proliferation change,and pi staining to detect cell cycle change.after sentrin-specific protease 1(senp1)overexpression vector was constructed,western blot was performed to detect the phosphorylation of p65 and inhibitory subunit-κbα(iκbα)from nf-κb pathway in con,rps9-shrna,and rps9-shrna-senp1 cells;in addition,annexin v/pi double staining was also performed to detect the apoptosis in these three cells. |
2019-08-20 |
2023-08-13 |
human |
| Kun Peng Song, Peng Shu, Ju Lei Ma, Bing Wang, Bo Che. [Ribosomal Protein S9 Expression in Multiple Myeloma and Its Effect on Cell Biological Function]. Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae. vol 41. issue 2. 2019-08-20. PMID:31060671. |
results the relative expression of rps9 in con group and cd138+group was(1.00±0.12)and(5.45±0.71),respectively(t=4.291,p=0.0036).western blot showed rps9 expression was high in most myeloma cd138+cells.the high expression of rps9 was associated with both extramedullary invasion and overall survival in gse19784 dataset.after rpmi8226 was infected with con or rps9-shrna lentivirus for 48 hours,flow cytometry confirmed that the infection efficiencies were above 90% in both groups.qpcr and western blot confirmed that rps9 expression was inhibited at both mrna and protein levels.after rpmi8226 con and rps9-shrna infected with virus for 48 hours,the proportion of annexin v-positive cells in con and rps9-shrna cells was(3.47±0.37)% and(18.60±64.00)%(t=9.015,p=0.0008).the proliferation index significantly differed between con group and rps9-shrna group at 72 hours(t=6.846,p=0.0024).when con and rps9-shrna were infected with virus for 48 hours,the proportion of g2 phase cells was(29.28±3.42)% and(10.43±1.43)%,respectively(t=9.329,p=0.0007).the rps9 expression was positively correlated with senp1 in gse19784 dataset and negatively correlated with iκbα coding gene nfkbia.western blot further confirmed that rps9 knockdown inhibited the expression of senp1,inhibited the phosphorylation of nf-κb subunit p65 and inhibitor iκbα,and promoted the expression of iκbα.overexpression of senp1 not only impeded this effect but also reduced rps9-induced apoptosis. |
2019-08-20 |
2023-08-13 |
human |
| Chao Ji, Ju Lei Ma, Peng Sh. [Effect of Denticleless E3 Ubiquitin Protein Ligase on Proliferation and Colone Formation of Multiple Myeloma Cells]. Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae. vol 41. issue 2. 2019-08-20. PMID:31060674. |
methods mononuclear cells were extracted from 34 mm patients.mononuclear cells harvested from 14 healthy volunteers were used as controls.quantitative polymerase chain reaction was used to detect the change of dtl at mrna level.furthermore,12 mm patients and 2 controls were selected,in whom the change of dtl at protein level was detected by western blot.human mm cell line rpmi8226 was divided into control(con)group and dtl-short hairpin rna(dtl-shrna)group,which was infected with the con and dtl-shrna virus,respectively,for 48 hours.the infection efficiency was detected by using flow cytometry,the knock-down efficiencies at mrna and protein levels were detected by quantitative polymerase chain reaction and western blot,the change of cell counts in the next 0,24,48,72,96 hours were measured with cck8 assay.the con and dtl-shrna cells were cultured in semisolid medium.ten days later,inverted phase microscopy was used to measure the number of colones that contain more than 50 cells,annexin v/propidium iodide double staining to detect apoptosis,and propidium iodide staning to detect cell cycle.finally,western blot was empoyed to detect the phosphorylation of p65 and inhibitory subunit-κbα(iκbα)in nuclear factor-κb(nf-κb)pathway and electrophoretic mobility shift assay(emsa)to detect the nf-κb transcriptional ability. |
2019-08-20 |
2023-08-13 |
human |
| Yanbo Qu, Zhixin Liao, Xinzhu Wang, Jing Zhang, Chao Li. EFLDO sensitizes liver cancer cells to TNFSF10‑induced apoptosis in a p53‑dependent manner. Molecular medicine reports. vol 19. issue 5. 2019-08-19. PMID:30896802. |
treatment with efldo sensitized liver cancer cells to tnfsf10, and apoptosis was induced via the upregulation of tnf receptor superfamily member 10a (tnfrsf10a) and tnfrsf10b in a tumor protein p53 (p53)‑dependent manner, as detected by reverse transcription‑quantitative polymerase chain reaction and western blot analyses. |
2019-08-19 |
2023-08-13 |
Not clear |
| J Cong, R Liu, J Hou, X Wang, H Jiang, J Wan. Effects of trastuzumab on the proliferation and apoptosis of ovarian cancer cells. Neoplasma. vol 66. issue 2. 2019-08-15. PMID:30509111. |
proliferation and apoptosis of skov3 cells were then measured by mts assay and flow cytometry and cleaved caspase-3 expression was detected by western blot. |
2019-08-15 |
2023-08-13 |
human |
| Khadijeh Bijangi-Vishehsaraei, M Reza Saadatzadeh, Haiyan Wang, Angie Nguyen, Malgorzata M Kamocka, Wenjing Cai, Aaron A Cohen-Gadol, Stacey L Halum, Jann N Sarkaria, Karen E Pollok, Ahmad R Saf. Sulforaphane suppresses the growth of glioblastoma cells, glioblastoma stem cell-like spheroids, and tumor xenografts through multiple cell signaling pathways. Journal of neurosurgery. vol 127. issue 6. 2019-08-14. PMID:28059653. |
methods the inhibitory effects of sfn on established cell lines, early primary cultures, cd133-positive gscs, gsc-derived spheroids, and gbm xenografts were evaluated using various methods, including gsc isolation and the sphere-forming assay, analysis of reactive oxygen species (ros) and apoptosis, cell growth inhibition assay, comet assays for assessing sfn-triggered dna damage, confocal microscopy, western blot analysis, and the determination of in vivo efficacy as assessed in human gbm xenograft models. |
2019-08-14 |
2023-08-13 |
human |
| Jingjing Zhang, Changlei Cui, Yanhui Li, Haiyang X. Inhibition of GSK-3beta Signaling Pathway Rescues Ketamine-Induced Neurotoxicity in Neural Stem Cell-Derived Neurons. Neuromolecular medicine. vol 20. issue 1. 2019-08-13. PMID:29218434. |
keta (50 μm)-induced neurotoxicity in cultured nscns was monitored by apoptosis, immunohistochemical and western blot assays, respectively. |
2019-08-13 |
2023-08-13 |
Not clear |
| Jie Li, Dan Feng, Cuixia Gao, Yingyi Zhang, Jing Xu, Meihong Wu, Xianbao Zha. Isoforms S and L of MRPL33 from alternative splicing have isoform‑specific roles in the chemoresponse to epirubicin in gastric cancer cells via the PI3K/AKT signaling pathway. International journal of oncology. vol 54. issue 5. 2019-08-13. PMID:30816492. |
furthermore, western blot analysis indicated that mrpl33‑s promoted the chemoresponse to epirubicin by deactivating pi3k/akt/creb signaling and inducing apoptosis, while mrpl33‑l had the opposite effects. |
2019-08-13 |
2023-08-13 |
Not clear |
| Kotaro Makita, Hiroyuki Hara, Emiko Sano, Yutaka Okamoto, Yushi Ochiai, Tomonori Harada, Takuya Ueda, Tomohiro Nakayama, Shin Aizawa, Atsuo Yoshin. Interferon-β sensitizes human malignant melanoma cells to temozolomide-induced apoptosis and autophagy. International journal of oncology. vol 54. issue 5. 2019-08-13. PMID:30864696. |
the expression of proteins involved in the cell cycle, apoptosis and autophagy was evaluated by western blot analysis. |
2019-08-13 |
2023-08-13 |
human |
| Jun Liu, Qingqing Li, Xiaoxin Li, Zhenzhen Qiu, Andrew Li, Wenhan Liang, Huangyao Chen, Xiangsheng Cai, Xinglu Chen, Xinyue Duan, Jingjing Li, Wangsheng Wu, Mingyu Xu, Yingying Mao, Huiying Chen, Jinlong Li, Weiwang Gu, Hongwei L. Zika Virus Envelope Protein induces G2/M Cell Cycle Arrest and Apoptosis via an Intrinsic Cell Death Signaling Pathway in Neuroendocrine PC12 Cells. International journal of biological sciences. vol 14. issue 9. 2019-08-12. PMID:29989100. |
flow cytometry and western blot analysis showed that the apoptosis was associated with up-regulation of both p53 and p21 |
2019-08-12 |
2023-08-13 |
Not clear |