| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| K Dimas, C Demetzos, V Vaos, P Ioannidis, T Tranga. Labdane type diterpenes down-regulate the expression of c-Myc protein, but not of Bcl-2, in human leukemia T-cells undergoing apoptosis. Leukemia research. vol 25. issue 6. 2001-06-28. PMID:11337016. |
western blot analysis showed that of c-myc proto-oncogene levels were markedly reduced before massive apoptosis ensued in h33aj-ja1 and molt3 cells, while bcl-2 expression remained unaffected. |
2001-06-28 |
2023-08-12 |
human |
| B Montaner, R Pérez-Tomá. Prodigiosin-induced apoptosis in human colon cancer cells. Life sciences. vol 68. issue 17. 2001-06-14. PMID:11388704. |
dld-1 and sw-620 human colon adenocarcinoma cells, nrk and swiss-3t3 nonmalignant cells were assayed by the mtt assay, fragmentation pattern of dna, hoechst 33342 staining and study of parp cleavage by western blot, in order to characterize the prodigiosin-induced apoptosis. |
2001-06-14 |
2023-08-12 |
human |
| P E Mirkes, S A Little, C C Umpierr. Co-localization of active caspase-3 and DNA fragmentation (TUNEL) in normal and hyperthermia-induced abnormal mouse development. Teratology. vol 63. issue 3. 2001-06-07. PMID:11283970. |
previous work has shown that caspase-3 activation and dna fragmentation, two hallmarks of apoptosis, are induced in day 9 mouse embryos exposed to hyperthermia (43 degrees c); however, the methods used to assess caspase-3 activation (western blot) and dna fragmentation (gel electrophoresis) did not allow these apoptotic events to be localized to specific cells within the embryo. |
2001-06-07 |
2023-08-12 |
mouse |
| J G Seol, W H Park, E S Kim, C W Jung, J M Hyun, Y Y Lee, B K Ki. Potential role of caspase-3 and -9 in arsenic trioxide-mediated apoptosis in PCI-1 head and neck cancer cells. International journal of oncology. vol 18. issue 2. 2001-05-24. PMID:11172589. |
to address the mechanism of apoptosis, a western blot assay was performed, showing that bax was up-regulated without a change in bcl-2. |
2001-05-24 |
2023-08-12 |
mouse |
| C X Guo, J Ma, X C Zhou, Y X Li. Expression of HSP70-2 gene during germ cell apoptosis in rat unilateral cryptorchid testes. Archives of andrology. vol 46. issue 2. 2001-05-21. PMID:11297064. |
to investigate the role of hsp70-2 gene in germ cell apoptosis induced by heat stress, its expression changes were examined in rat normal and unilateral cryptorchid testes by using in situ hybridization, immunohistochemistry, and northern blot analysis techniques. |
2001-05-21 |
2023-08-12 |
rat |
| G W Wang, Z Zhou, J B Klein, Y J Kan. Inhibition of hypoxia/reoxygenation-induced apoptosis in metallothionein-overexpressing cardiomyocytes. American journal of physiology. Heart and circulatory physiology. vol 280. issue 5. 2001-05-17. PMID:11299233. |
in association with apoptosis, mitochondrial cytochrome c release, as determined by western blot, was observed to occur in nontransgenic cardiomyocytes. |
2001-05-17 |
2023-08-12 |
mouse |
| Z Zhou, S F Jia, M C Hung, E S Kleinerma. E1A sensitizes HER2/neu-overexpressing Ewing's sarcoma cells to topoisomerase II-targeting anticancer drugs. Cancer research. vol 61. issue 8. 2001-05-10. PMID:11309298. |
e1a-induced apoptosis was demonstrated by both flow cytometric analysis and western blot analysis using a poly(adp-ribose) polymerase antibody. |
2001-05-10 |
2023-08-12 |
human |
| A Lansiaux, W Laine, B Baldeyrou, C Mahieu, N Wattez, H Vezin, F J Martinez, A Piñeyro, C Baill. DNA topoisomerase II inhibition by peroxisomicine A(1) and its radical metabolite induces apoptotic cell death of HL-60 and HL-60/MX2 human leukemia cells. Chemical research in toxicology. vol 14. issue 1. 2001-04-26. PMID:11170504. |
the cytometry and western blot analyses reveal that peroxisomicine induces apoptosis in leukemia cells. |
2001-04-26 |
2023-08-12 |
human |
| E Engidawork, T Gulesserian, B C Yoo, N Cairns, G Lube. Alteration of caspases and apoptosis-related proteins in brains of patients with Alzheimer's disease. Biochemical and biophysical research communications. vol 281. issue 1. 2001-04-05. PMID:11178964. |
to further substantiate the role of apoptosis in ad, we investigated the levels of nine different proteins involved in apoptosis by western blot technique in frontal cortex and cerebellum of control and ad subjects. |
2001-04-05 |
2023-08-12 |
human |
| K H Choi, T I Kim, D L Chong, H Y Lee, D S Ha. Gentamicin induced apoptosis of renal tubular epithelial (LLC-PK1) cells. The Korean journal of internal medicine. vol 15. issue 3. 2001-03-22. PMID:11242810. |
however, the 15-day gm treatment did not cause a significant elevation in the expression of the 45 kd fas protein, the cell surface molecule that stimulates apoptosis, by western blot analysis. |
2001-03-22 |
2023-08-12 |
Not clear |
| P Mercié, F Bello. [Physicopathologic mechanisms and methods of analysis of cellular apoptosis]. La Revue de medecine interne. vol 22. issue 1. 2001-03-08. PMID:11218312. |
the methods of identification of the apoptosis pathways are: morphological modifications observed in microscopy, the evaluation of the difference of the mitochondrial membrane potential, the measurement of the devdase activity, the labelling of the phosphatidylserines by the annexin v on the cell surface, and the western blot allowing the identification of the activated caspases. |
2001-03-08 |
2023-08-12 |
human |
| M Artwohl, T Hölzenbein, L Wagner, A Freudenthaler, W Waldhäusl, S M Baumgartner-Parze. Levamisole induced apoptosis in cultured vascular endothelial cells. British journal of pharmacology. vol 131. issue 8. 2001-02-22. PMID:11139434. |
cells exposed (24 h) to levamisole (range: 0.5 - 2 mmol l(-1)) alone or in combination with antioxidants (10 mmol l(-1) glutathione or 5 mmol l(-1) n-acetylcysteine or 0.1 mmol l(-1) tocopherol) were evaluated for apoptosis ((3)h-thymidine assays, in situ staining), mrna/protein expression (northern/western blot), and proliferation ((3)h-thymidine incorporation). |
2001-02-22 |
2023-08-12 |
human |
| F Zhu, W Yan, Z L Zhao, Y B Chai, F Lu, Q Wang, W D Peng, A G Yang, C J Wan. Improved PCR-based subtractive hybridization strategy for cloning differentially expressed genes. BioTechniques. vol 29. issue 2. 2001-02-01. PMID:10948432. |
twenty-seven clones related to apoptosis were identified by reverse dot blot assay. |
2001-02-01 |
2023-08-12 |
human |
| F Zhu, W Yan, Z L Zhao, Y B Chai, F Lu, Q Wang, W D Peng, A G Yang, C J Wan. Improved PCR-based subtractive hybridization strategy for cloning differentially expressed genes. BioTechniques. vol 29. issue 2. 2001-02-01. PMID:10948432. |
apr-1, apr-2, apr-3 and tnf were reidentified by reverse dot blot, and it is suggested that they might be related to apoptosis. |
2001-02-01 |
2023-08-12 |
human |
| K C Wang, A L Cheng, S E Chuang, H C Hsu, I J S. Retinoic acid-induced apoptotic pathway in T-cell lymphoma: Identification of four groups of genes with differential biological functions. Experimental hematology. vol 28. issue 12. 2001-01-25. PMID:11146166. |
the cloned gene fragments were then analyzed and confirmed by northern blot analysis on h9 and sr786 cell lines.atra-induced apoptosis of t-cell lymphoma was protein synthesis-dependent. |
2001-01-25 |
2023-08-12 |
Not clear |
| Q N Diep, R M Touyz, E L Schiffri. Docosahexaenoic acid, a peroxisome proliferator-activated receptor-alpha ligand, induces apoptosis in vascular smooth muscle cells by stimulation of p38 mitogen-activated protein kinase. Hypertension (Dallas, Tex. : 1979). vol 36. issue 5. 2000-12-28. PMID:11082155. |
to clarify possible pathways of apoptosis, we evaluated the expression of phosphorylated p38 mitogen-activated protein kinases, bax, bcl-2, cytochrome c, and peroxisome proliferator-activated receptor-alpha (ppar-alpha) with western blot analysis. |
2000-12-28 |
2023-08-12 |
rat |
| R Moriya, T Uehara, Y Nomur. Mechanism of nitric oxide-induced apoptosis in human neuroblastoma SH-SY5Y cells. FEBS letters. vol 484. issue 3. 2000-12-22. PMID:11078888. |
western blot analysis showed that pro-forms of caspase-2, -3, -6, and -7 are decreased during apoptosis. |
2000-12-22 |
2023-08-12 |
human |
| J J Lysiak, S D Turner, T T Turne. Molecular pathway of germ cell apoptosis following ischemia/reperfusion of the rat testis. Biology of reproduction. vol 63. issue 5. 2000-12-15. PMID:11058553. |
proteins from seminiferous tubules were used for western blot analysis of cytochrome c. germ cell apoptosis was maximal at 24 h after repair of torsion. |
2000-12-15 |
2023-08-12 |
rat |
| J C Shen, R D Klein, Q Wei, Y Guan, J H Contois, T T Wang, S Chang, S D Hurstin. Low-dose genistein induces cyclin-dependent kinase inhibitors and G(1) cell-cycle arrest in human prostate cancer cells. Molecular carcinogenesis. vol 29. issue 2. 2000-11-30. PMID:11074606. |
viable cell number was assessed by the 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay; cell-cycle progression and apoptosis were evaluated by flow cytometry; apoptosis was also assessed by a histone enzyme-linked immunosorbent assay; and the expression of several cell-cycle- and apoptosis-related genes and their gene products was determined by northern blot analysis, western blot analysis, and/or assays based on polymerase chain reaction. |
2000-11-30 |
2023-08-12 |
human |
| H Sasaki, Y Sheng, F Kotsuji, B K Tsan. Down-regulation of X-linked inhibitor of apoptosis protein induces apoptosis in chemoresistant human ovarian cancer cells. Cancer research. vol 60. issue 20. 2000-11-08. PMID:11059757. |
apoptosis and protein expression (e.g., xiap, caspase-3, p53, mdm2, and p21waf1) were assessed by hoechst 33258 stain and western blot, respectively. |
2000-11-08 |
2023-08-12 |
human |