| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| T E Vaskivuo, F Stenbäck, P Karhumaa, J Risteli, L Dunkel, J S Tapanaine. Apoptosis and apoptosis-related proteins in human endometrium. Molecular and cellular endocrinology. vol 165. issue 1-2. 2000-11-07. PMID:10940486. |
apoptosis was studied quantitatively by southern blot analysis of internucleosomal cleavage of genomic dna, and qualitatively by using in situ 3'-end labeling of fragmented dna, and bcl-2, bax and ki-67 were detected and quantified immunohistochemically. |
2000-11-07 |
2023-08-12 |
human |
| L S Wang, H J Liu, Z B Xia, H E Broxmeyer, L L. Expression and activation of caspase-3/CPP32 in CD34(+) cord blood cells is linked to apoptosis after growth factor withdrawal. Experimental hematology. vol 28. issue 8. 2000-10-10. PMID:10989191. |
reverse transcriptase-polymerase chain reaction (rt-pcr), western blot, and flow cytometry analysis were used in this study to determine the expression of caspase-3/cpp32 in cd34(+) cb cells during apoptosis. |
2000-10-10 |
2023-08-12 |
Not clear |
| N Mitsiades, V Poulaki, S Tseleni-Balafouta, D A Koutras, I Stamenkovi. Thyroid carcinoma cells are resistant to FAS-mediated apoptosis but sensitive to tumor necrosis factor-related apoptosis-inducing ligand. Cancer research. vol 60. issue 15. 2000-08-31. PMID:10945619. |
we addressed the expression of fas, dr4, and dr5 in thyroid carcinoma cell lines and in 31 thyroid carcinoma specimens by western blot analysis and immunohistochemistry, respectively, and tested the sensitivity of thyroid carcinoma cell lines to fas- and trail-induced apoptosis. |
2000-08-31 |
2023-08-12 |
Not clear |
| D Dai, B C Moulton, T F Ogl. Regression of the decidualized mesometrium and decidual cell apoptosis are associated with a shift in expression of Bcl2 family members. Biology of reproduction. vol 63. issue 1. 2000-08-24. PMID:10859259. |
expression of bax and bcl2 and the incidence of apoptosis were evaluated throughout gestation by western blot analysis and detection of dna fragments. |
2000-08-24 |
2023-08-12 |
rat |
| J Xu, Z Xu, Y Jiang, X Qian, Y Huan. Cryptorchidism induces mouse testicular germ cell apoptosis and changes in bcl-2 and bax protein expression. Journal of environmental pathology, toxicology and oncology : official organ of the International Society for Environmental Toxicology and Cancer. vol 19. issue 1-2. 2000-08-22. PMID:10905505. |
western blot analysis showed that the bcl-2 protein down-regulated and the bax protein up-regulated apoptosis. |
2000-08-22 |
2023-08-12 |
mouse |
| W J Zhang, K Ohnishi, H Yoshida, L Pan, L Maksumova, F Muratkhodjaev, J M Luo, K Shigeno, S Fujisawa, K Naito, S Nakamura, K Shinjo, A Takeshita, R Ohn. Spicamycin and KRN5500 induce apoptosis in myeloid and lymphoid cell lines with down-regulation of bcl-2 expression and modulation of promyelocytic leukemia protein. Japanese journal of cancer research : Gann. vol 91. issue 6. 2000-08-18. PMID:10874212. |
western blot analysis of bcl-2, bcl-xl and bax expression and immunofluorescence analysis of promyelocytic leukemia (pml) protein indicated that apoptosis induced by spicamycin and krn5500 was associated with down-regulation of bcl-2 expression and modulation of pml protein. |
2000-08-18 |
2023-08-12 |
mouse |
| A P Simões-Wüst, R A Olie, O Gautschi, S H Leech, R Häner, J Hall, D Fabbro, R A Stahel, U Zangemeister-Wittk. Bcl-xl antisense treatment induces apoptosis in breast carcinoma cells. International journal of cancer. vol 87. issue 4. 2000-08-18. PMID:10918201. |
the antisense effect of oligonucleotide 4259 was examined on the mrna and protein level using real-time pcr and western blot analysis, respectively, and the induction of cell death was investigated in viability and apoptosis assays. |
2000-08-18 |
2023-08-12 |
Not clear |
| T L Beumer, H L Roepers-Gajadien, I S Gademan, T M Lock, H B Kal, D G De Rooi. Apoptosis regulation in the testis: involvement of Bcl-2 family members. Molecular reproduction and development. vol 56. issue 3. 2000-08-09. PMID:10862001. |
using immunohistochemical techniques and western blot analysis, the possible role of bcl-2 family members bax, bcl-2, bcl-x(s), and bcl-x(l) in male germ cell density-related apoptosis and dna damage induced apoptosis was studied. |
2000-08-09 |
2023-08-12 |
mouse |
| V M González, M A Fuertes, M J Pérez-Alvarez, G Cervantes, V Moreno, C Alonso, J M Pére. Induction of apoptosis by the bis-Pt(III) complex [Pt(2)(2-mercaptopyrimidine)(4)Cl(2)]. Biochemical pharmacology. vol 60. issue 3. 2000-07-13. PMID:10856432. |
interestingly, western blot data indicated that apoptosis induction by pt-spym in hela, ch1, and ch1cisr cells was not associated with drastic changes in p53 levels. |
2000-07-13 |
2023-08-12 |
human |
| N Baghdassarian, Y Bertrand, P Ffrench, P Duhaut, P A Bryon, M Ffrenc. Role of BCL-2 and cell cycle regulatory proteins for corticosensitivity assessment in childhood acute lymphoblastic leukaemia. British journal of haematology. vol 109. issue 1. 2000-07-12. PMID:10848789. |
fifty-two children presenting with all were studied at diagnosis and during the first 48 h of treatment for cell proliferation and apoptosis level by measurement of dna content, and for expression of several cell proliferation regulatory proteins by means of western blot. |
2000-07-12 |
2023-08-12 |
Not clear |
| S J Baker, E P Redd. Cloning of murine G1RP, a novel gene related to Drosophila melanogaster g1. Gene. vol 248. issue 1-2. 2000-07-06. PMID:10806348. |
northern blot analysis using rnas derived from 32dcl3 cells that have been grown in the absence of il-3 demonstrates that the g1rp message is upregulated in these cells following the removal of il-3, suggesting that this gene may regulate growth factor withdrawal-induced apoptosis of myeloid precursor cells. |
2000-07-06 |
2023-08-12 |
drosophila_melanogaster |
| A Zaragoza, C Díez-Fernández, A M Alvarez, D Andrés, M Cascale. Effect of N-acetylcysteine and deferoxamine on endogenous antioxidant defense system gene expression in a rat hepatocyte model of cocaine cytotoxicity. Biochimica et biophysica acta. vol 1496. issue 2-3. 2000-06-29. PMID:10771087. |
lysis of the cell membranes was evidenced by lactate dehydrogenase leakage, apoptosis was observed by detecting a hypodiploid peak (<2c) in dna histograms obtained by flow cytometry, peroxide production was quantified with 2', 7'-dichlorodihydrofluorescein diacetate and gene expression of the antioxidant enzymes: mn- and cu,zn-superoxide dismutases, catalase and glutathione peroxidase were measured by northern blot analysis. |
2000-06-29 |
2023-08-12 |
rat |
| Y Akao, H Yamada, Y Nakagaw. Arsenic-induced apoptosis in malignant cells in vitro. Leukemia & lymphoma. vol 37. issue 1-2. 2000-05-18. PMID:10721769. |
in addition to the involvement of caspase 3 in arsenic trioxide-induced apoptosis of nb4 cells, the activation of caspase 8 was also shown to be involved by western blot analysis or by apoptosis inhibition assay using caspase 8 inhibitor ac-ietd-cho. |
2000-05-18 |
2023-08-12 |
mouse |
| S B Brown, M C Clarke, L Magowan, H Sanderson, J Savil. Constitutive death of platelets leading to scavenger receptor-mediated phagocytosis. A caspase-independent cell clearance program. The Journal of biological chemistry. vol 275. issue 8. 2000-03-30. PMID:10681593. |
however, and in contrast with constitutive death of other inflammatory blood cells by apoptosis, these events were not affected by caspase inhibitors, nor was there evidence of caspase-3 activation either by hydrolysis of analog peptide substrates or western blot analysis, serving to emphasize that neither programmed cell death nor clearance by phagocytes need involve caspases. |
2000-03-30 |
2023-08-12 |
Not clear |
| A M Rodríguez-López, O Flores, C Martínez-Salgado, N Eleno, J M López-Novoa, M Aréval. Increased apoptosis susceptibility in mesangial cells from spontaneously hypertensive rats. Microvascular research. vol 59. issue 1. 2000-02-25. PMID:10625574. |
we assessed apoptosis by propidium iodide staining, by tunel nitrite production (griess reaction), by inducible nitric oxide synthase (inos) and bcl-2 and bax by western blot. |
2000-02-25 |
2023-08-12 |
rat |
| Y L Sun, Y Zhao, X Hong, Z H Zha. Cytochrome c release and caspase activation during menadione-induced apoptosis in plants. FEBS letters. vol 462. issue 3. 2000-02-02. PMID:10622718. |
western blot analysis indicated that the caspase specific inhibitors ac-devd-cho (ac-asp-glu-val-asp-aldehyde) and ac-yvad-cho (n-acetyl-try-val-ala-aspartinal) inhibited the degradation of a caspase 3 specific substrate parp (poly(adp-ribose) polymerase), and they had no effect on the release of cytochrome c. further study showed that menadione could not induce apoptosis of mouse liver nuclei in tobacco cytosol extract containing no mitochondria. |
2000-02-02 |
2023-08-12 |
mouse |
| L y Xue, J He, N L Oleinic. Promotion of photodynamic therapy-induced apoptosis by stress kinases. Cell death and differentiation. vol 6. issue 9. 2000-01-04. PMID:10510467. |
to ascertain whether activation of one or more mapks could mediate pdt-induced apoptosis, western blot analysis has been performed on the proteins of ly-r and cho cells at various times following lethal (90 - 99% cell kill) doses of pdt photosensitized by the phthalocyanine pc 4. |
2000-01-04 |
2023-08-12 |
Not clear |
| J H Richburg, A Nañez, H Ga. Participation of the Fas-signaling system in the initiation of germ cell apoptosis in young rat testes after exposure to mono-(2-ethylhexyl) phthalate. Toxicology and applied pharmacology. vol 160. issue 3. 1999-12-09. PMID:10544061. |
western blot analysis demonstrated a time-dependent increase in the production of the soluble form of fasl after mehp exposure and suggests that it may participate in triggering apoptosis in germ cells that have lost their intimate association with the sertoli cells. |
1999-12-09 |
2023-08-12 |
rat |
| K Erkkilä, V Pentikäinen, M Wikström, M Parvinen, L Dunke. Partial oxygen pressure and mitochondrial permeability transition affect germ cell apoptosis in the human testis. The Journal of clinical endocrinology and metabolism. vol 84. issue 11. 1999-11-26. PMID:10566681. |
apoptosis was significantly suppressed in an inversely dose-dependent fashion at partial oxygen pressures below 10%, as detected by southern blot analysis of dna fragmentation, dna labeling in situ, and electron microscopy. |
1999-11-26 |
2023-08-12 |
human |
| I Petrache, M E Choi, L E Otterbein, B Y Chin, L L Mantell, S Horowitz, A M Cho. Mitogen-activated protein kinase pathway mediates hyperoxia-induced apoptosis in cultured macrophage cells. The American journal of physiology. vol 277. issue 3. 1999-10-28. PMID:10484467. |
to further delineate the signaling pathway of hyperoxia-induced apoptosis in raw 264.7 macrophages, we first show that hyperoxia can activate the mitogen-activated protein kinase (mapk) pathway, the extracellular signal-regulated kinases (erks) p42/p44, in a time-dependent manner as assessed by increased phosphorylation of erk1/erk2 by western blot analyses. |
1999-10-28 |
2023-08-12 |
mouse |