| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Beatriz Bellosillo, Neus Villamor, Armando López-Guillermo, Silvia Marcé, Francesc Bosch, Elias Campo, Emili Montserrat, Dolors Colome. Spontaneous and drug-induced apoptosis is mediated by conformational changes of Bax and Bak in B-cell chronic lymphocytic leukemia. Blood. vol 100. issue 5. 2002-09-13. PMID:12176904. |
following drug-induced apoptosis, bax integrates into mitochondria, as demonstrated by fluorescence microscopy and western blot, without changes in the total amount of bax or bak protein. |
2002-09-13 |
2023-08-12 |
Not clear |
| H L Li, K H Ye, H W Zhang, Y R Luo, X D Ren, A H Xiong, R Sit. Effect of heparin on apoptosis in human nasopharyngeal carcinoma CNE2 cells. Cell research. vol 11. issue 4. 2002-08-06. PMID:11787776. |
in order to study the mechanism of the effect of heparin on apoptosis in carcinoma cells, the nasopharyngeal carcinoma cell line cne2 was used to identify the effect of heparin on apoptosis associated with the expression of c-myc, bax, bcl-2 proteins by use of hoechst 33258 staining, terminal deoxynucleotidyl transferase-mediated dutp nick-end labeling (tunel), agarose gel electrophoresis, and flow cytometry, as well as western blot analysis. |
2002-08-06 |
2023-08-12 |
human |
| Kristina G Hobson, Kiho Cho, Lee K Adamson, David G Greenhalg. Burn-induced thymic apoptosis corresponds with altered TGF-beta(1) and Smad 2/3. The Journal of surgical research. vol 105. issue 1. 2002-07-26. PMID:12069494. |
tgf-beta(1) and smad 2/3 expression and levels of apoptosis were determined in whole tissue and in sorted t-cells by flow cytometry, rt-pcr, elisa, and western blot. |
2002-07-26 |
2023-08-12 |
mouse |
| Zhihu Ding, Shou-Ching Tang, Priya Weerasinghe, Xiaolong Yang, Alan Pater, Andrejs Liepin. The alkaloid sanguinarine is effective against multidrug resistance in human cervical cells via bimodal cell death. Biochemical pharmacology. vol 63. issue 8. 2002-07-15. PMID:11996882. |
treatment with 2.12 or 4.24 microm sanguinarine induced death in most cells that was characterized as apoptosis using the criteria of cell surface blebbing, as determined by light and scanning electron microscopy, and proteolytic activation of caspase-3 and cleavage of the caspase-3 substrate poly(adp-ribose) polymerase (parp), as detected by western blot analysis. |
2002-07-15 |
2023-08-12 |
human |
| P Weerasinghe, S Hallock, S C Tang, A Liepin. Role of Bcl-2 family proteins and caspase-3 in sanguinarine-induced bimodal cell death. Cell biology and toxicology. vol 17. issue 6. 2002-06-21. PMID:11787859. |
here, we report that sanguinarine-treated k562 cells, when analyzed by western blot, showed significant increase in expression of the pro-apoptotic bax protein in apoptosis, but not in bcd. |
2002-06-21 |
2023-08-12 |
human |
| Giovanna Andreola, Licia Rivoltini, Chiara Castelli, Veronica Huber, Paola Perego, Paola Deho, Paola Squarcina, Paola Accornero, Francesco Lozupone, Luana Lugini, Annarita Stringaro, Agnese Molinari, Giuseppe Arancia, Massimo Gentile, Giorgio Parmiani, Stefano Fai. Induction of lymphocyte apoptosis by tumor cell secretion of FasL-bearing microvesicles. The Journal of experimental medicine. vol 195. issue 10. 2002-06-19. PMID:12021310. |
isolated melanosomes express fasl, as detected by western blot and cytofluorimetry, and they can exert fas-mediated apoptosis in jurkat cells. |
2002-06-19 |
2023-08-12 |
Not clear |
| E Engidawork, N Balic, J F Juranville, M Fountoulakis, M Dierssen, G Lube. Unaltered expression of Fas (CD95/APO-1), caspase-3, Bcl-2 and annexins in brains of fetal Down syndrome: evidence against increased apoptosis. Journal of neural transmission. Supplementum. issue 61. 2002-06-10. PMID:11771740. |
to address this issue, we investigated the expression of proteins involved in apoptosis including fas (cd95, apo-1), caspase-3, bcl-2 and annexins in the cerebral cortex of control and ds fetal brain by western blot and two dimensional electrophoresis. |
2002-06-10 |
2023-08-12 |
Not clear |
| E Engidawork, T Gulesserian, R Seidl, N Cairns, G Lube. Expression of apoptosis related proteins: RAIDD, ZIP kinase, Bim/BOD, p21, Bax, Bcl-2 and NF-kappaB in brains of patients with Down syndrome. Journal of neural transmission. Supplementum. issue 61. 2002-06-10. PMID:11771742. |
to further substantiate the hypothesis that apoptosis is the underlying mechanism for neuronal loss and contribute towards the current knowledge of apoptosis in ds, we analyzed the expression of apoptosis related proteins in frontal cortex and cerebellum of ds by western blot and elisa techniques. |
2002-06-10 |
2023-08-12 |
Not clear |
| A Saxena, J D McMeekin, D J Thomso. Expression of Bcl-x, Bcl-2, Bax, and Bak in endarterectomy and atherectomy specimens. The Journal of pathology. vol 196. issue 3. 2002-06-10. PMID:11857498. |
in atherectomy (15), endarterectomy (10), and control non-atherosclerotic segments of renal (2) and of coronary and carotid (5) arteries, the extent of apoptosis was determined using tdt dutp nick end labelling (tunel) and nuclear morphology (karyorrhexis/pyknosis) and expression of apoptosis regulators by immunohistochemistry and western blot analysis on paraffin-embedded material. |
2002-06-10 |
2023-08-12 |
Not clear |
| Chibo Hong, Gary L Firestone, Leonard F Bjeldane. Bcl-2 family-mediated apoptotic effects of 3,3'-diindolylmethane (DIM) in human breast cancer cells. Biochemical pharmacology. vol 63. issue 6. 2002-06-10. PMID:11931841. |
furthermore, western and northern blot analyses, as well as coimmunoprecipitation assays, revealed that in both mcf-7 and mda-mb-231 cells, dim treatment decreased total transcript and protein levels of the apoptosis inhibitory protein bcl-2, and the amount of bcl-2 bound to the pro-apoptotic protein bax. |
2002-06-10 |
2023-08-12 |
human |
| Yoshihito Nakagawa, Yukihiro Akao, Hiroshi Morikawa, Ichiro Hirata, Kenichi Katsu, Tomoki Naoe, Nobuko Ohishi, Kunio Yag. Arsenic trioxide-induced apoptosis through oxidative stress in cells of colon cancer cell lines. Life sciences. vol 70. issue 19. 2002-05-23. PMID:12005185. |
the apoptosis was executed by the activation of caspase 3, which was shown by western blot, enzymatic activity, and apoptosis inhibition assay. |
2002-05-23 |
2023-08-12 |
Not clear |
| S M Ali, S K Chee, G Y Yuen, M Oliv. Hypericin and hypocrellin induced apoptosis in human mucosal carcinoma cells. Journal of photochemistry and photobiology. B, Biology. vol 65. issue 1. 2002-05-14. PMID:11748006. |
under apoptotic conditions, western blot analysis of poly(adp-ribose) polymerase, a caspases substrate, showed the classical cleavage pattern (116 to 85 kda) associated with apoptosis in ha, hb and hy-treated cell lysates. |
2002-05-14 |
2023-08-12 |
human |
| Maeve Sheehan, Hector R Wong, Paul W Hake, Vivek Malhotra, Michael O'Connor, Basilia Zingarell. Parthenolide, an inhibitor of the nuclear factor-kappaB pathway, ameliorates cardiovascular derangement and outcome in endotoxic shock in rodents. Molecular pharmacology. vol 61. issue 5. 2002-05-13. PMID:11961112. |
immunohistochemistry showed positive staining for nitrotyrosine, poly(adp-ribose) synthetase (pars) and apoptosis, whereas northern blot analysis showed increased mrna expression of inducible nitric-oxide synthase (inos) in thoracic aortas. |
2002-05-13 |
2023-08-12 |
mouse |
| Antonio Ceriello, Lisa Quagliaro, Michele D'Amico, Clara Di Filippo, Raffaele Marfella, Francesco Nappo, Liberato Berrino, Francesco Rossi, Dario Giuglian. Acute hyperglycemia induces nitrotyrosine formation and apoptosis in perfused heart from rat. Diabetes. vol 51. issue 4. 2002-05-10. PMID:11916928. |
coronary perfusion pressure; no and superoxide anion generation; immunostaining for nt, inducible no synthase (inos), and the constitutive type of no synthase (nos) enos; inos and enos mrna expression by western blot and rt-pcr; and apoptosis of cardiac cells were studied in hearts perfused for 2 h with solutions containing d-glucose at a concentration of 11.1 mmol/l (control), d-glucose at the concentration of 33.3 mmol/l (high glucose), or d-glucose (33.3 mmol/l) plus glutathione (0.3 mmol/l). |
2002-05-10 |
2023-08-12 |
rat |
| Valérie Chopin, Robert-Alain Toillon, Nathalie Jouy, Xuefen Le Bourhi. Sodium butyrate induces P53-independent, Fas-mediated apoptosis in MCF-7 human breast cancer cells. British journal of pharmacology. vol 135. issue 1. 2002-04-26. PMID:11786482. |
western blot analysis showed that butyrate increased fas and fas ligand levels (fas l), indicating that butyrate-induced apoptosis may be mediated by fas signalling. |
2002-04-26 |
2023-08-12 |
human |
| S Hsu, J B Lewis, J L Borke, B Singh, D P Dickinson, G B Caughman, M Athar, L Drake, A C Aiken, C T Huynh, B R Das, T Osaki, G S Schuste. Chemopreventive effects of green tea polyphenols correlate with reversible induction of p57 expression. Anticancer research. vol 21. issue 6A. 2002-04-19. PMID:11911242. |
in this study, western blot analysis combined with cycloheximide treatment was used to examine the effects of green tea polyphenols on the expression levels of p57, a cyclin-dependent kinase and apoptosis inhibitor, in normal human keratinocytes and in the oral carcinoma cell lines scc25 and osc2. |
2002-04-19 |
2023-08-12 |
human |
| Linda Trotter, Wendy Panton, Iradj Hajimohamadreza, Lawrence Petalidis, Robin Ward, Yvonne Fleming, Christopher G Armstrong, Philip Cohen, Eric H Karran, Ross A Kinloc. Mitogen-activated protein kinase kinase 7 is activated during low potassium-induced apoptosis in rat cerebellar granule neurons. Neuroscience letters. vol 320. issue 1-2. 2002-04-18. PMID:11849756. |
western blot analysis revealed that levels of phosphorylated c-jun were elevated between 30 min and 10 h of cgn apoptosis, temporally consistent with mkk7 activation. |
2002-04-18 |
2023-08-12 |
rat |
| M A Esco, Z Wang, M L McDermott, M Kurpakus-Wheate. Potential role for laminin 5 in hypoxia-mediated apoptosis of human corneal epithelial cells. Journal of cell science. vol 114. issue Pt 22. 2002-04-10. PMID:11739635. |
increased apoptosis in hypoxic epithelium at 7 days in culture correlated with decreased deposition of laminin 5 into the extracellular matrix, as determined by western blot analysis and immunofluorescence microscopy. |
2002-04-10 |
2023-08-12 |
human |
| K Liu, J Husler, J Ye, S S Leonard, D Cutler, F Chen, S Wang, Z Zhang, M Ding, L Wang, X Sh. On the mechanism of Cr (VI)-induced carcinogenesis: dose dependence of uptake and cellular responses. Molecular and cellular biochemistry. vol 222. issue 1-2. 2002-02-27. PMID:11678606. |
the activation of transcription factors was assessed by electrophoretic mobility shift assay and western blot analysis, dna damage by single cell gel electrophoresis assay, cell apoptosis by dna fragmentation assay, and cell proliferation by a non-radioactive elisa kit. |
2002-02-27 |
2023-08-12 |
human |
| B Wen, E Deutsch, E Marangoni, V Frascona, L Maggiorella, B Abdulkarim, N Chavaudra, J Bourhi. Tyrphostin AG 1024 modulates radiosensitivity in human breast cancer cells. British journal of cancer. vol 85. issue 12. 2002-02-21. PMID:11747348. |
western blot analysis indicated that tyrphostin ag 1024-induced apoptosis was associated with a downregulation of expression of phospho-akt1, increased expression of bax, p53 and p21, and a decreased expression of bcl-2 expression, especially when combined with irradiation. |
2002-02-21 |
2023-08-12 |
human |