| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Yan-Ping Ma, Ping Zou, Juan Xiao, Shi-Ang Huan. Expression of caspase-3 in CD34+ cord blood cells and its significance. Zhongguo shi yan xue ye xue za zhi. vol 10. issue 5. 2003-08-12. PMID:12513733. |
to evaluate expression of caspase-3 protein in cd34(+) cells from cord blood (cb) and its significance during culture in vitro with various growth factors, rt-pcr, western blot and flow cytometry were used to determine cell proliferation, apoptosis and expression of caspase-3 in cd34(+) cells during culture in vitro. |
2003-08-12 |
2023-08-12 |
Not clear |
| Jian Li, Lu Zuo, Ti Shen, Zhi-Nan Zhan. [Inhibition of the activation of transcriptional factor NFkappaB during sodium selenite-induced apoptosis in NB4 cells]. Zhongguo shi yan xue ye xue za zhi. vol 10. issue 5. 2003-08-12. PMID:12513738. |
in order to evaluate the effect of sodium selenite on the activation of nfkappab during selenite-induced apoptosis in nb4 cells, western blot was used to measure the level of p65 in nuclear extraction of nb4 cells treated with sodium selenite to reflect the activation of nfkappab; the apoptosis of nb4 cells was determined by morphological observation, dna ladder electrophoresis and flow cytometry; and mtt test was used to measure the growth inhibition of cells. |
2003-08-12 |
2023-08-12 |
Not clear |
| Bingtuan Wang, Chaowu Xiao, Alan K Gof. Progesterone-modulated induction of apoptosis by interferon-tau in cultured epithelial cells of bovine endometrium. Biology of reproduction. vol 68. issue 2. 2003-08-01. PMID:12533434. |
the incidence of apoptosis was assessed by using tunel, dna fragmentation analysis, and western blot analysis of bax-alpha protein expression. |
2003-08-01 |
2023-08-12 |
cattle |
| Manchao Zhang, Hongpeng Liu, Ribo Guo, Yan Ling, Xiaojin Wu, Bihua Li, Peter P Roller, Shaomeng Wang, Dajun Yan. Molecular mechanism of gossypol-induced cell growth inhibition and cell death of HT-29 human colon carcinoma cells. Biochemical pharmacology. vol 66. issue 1. 2003-07-31. PMID:12818369. |
with a serial of western blot analysis, it is revealed that gossypol-induced cell cycle arrest is involved in p21 up-regulation and cyclin d1 down-regulation; gossypol-induced apoptosis triggers down-regulation of anti-apoptosis bcl-2 members: bcl-x(l), bag-1 and mcl-1, up-regulation of pro-apoptosis bcl-2 member bak, activation of caspase-3, -6, -7, -8, and -9, up-regulation of apaf-1, release of cytochrome c (cyto-c) from mitochondria, and activation of both dff45 and parp. |
2003-07-31 |
2023-08-12 |
human |
| S Willaime-Morawek, K Brami-Cherrier, J Mariani, J Caboche, B Brug. C-Jun N-terminal kinases/c-Jun and p38 pathways cooperate in ceramide-induced neuronal apoptosis. Neuroscience. vol 119. issue 2. 2003-07-30. PMID:12770554. |
western blot analyses of jnk and c-jun using phospho-specific antibodies reveal that jnk and subsequent c-jun phosphorylation occur hours before the initiation of apoptosis, reflected morphologically by neurite retraction and fragmentation, cell-body shrinkage and chromatin fragmentation. |
2003-07-30 |
2023-08-12 |
human |
| Xinbin Chen, Gang Liu, Jianhui Zhu, Jiayuan Jiang, Susan Nozell, Amy Willi. Isolation and characterization of fourteen novel putative and nine known target genes of the p53 family. Cancer biology & therapy. vol 2. issue 1. 2003-07-23. PMID:12673118. |
here, we used affymetrix genechip assay and northern blot analysis to determine whether some known target genes are regulated by various p53 mutants, which are active in inducing cell cycle arrest, apoptosis, or both, and to identify novel target genes regulated by the p53 family. |
2003-07-23 |
2023-08-12 |
Not clear |
| Amiya P Sinha Hikim, Yanhe Lue, Cindy M Yamamoto, Yanira Vera, Susana Rodriguez, Pauline H Yen, Kevin Soeng, Christina Wang, Ronald S Swerdlof. Key apoptotic pathways for heat-induced programmed germ cell death in the testis. Endocrinology. vol 144. issue 7. 2003-07-22. PMID:12810573. |
furthermore, early in apoptosis, a significant amount of bax also accumulates in endoplasmic reticulum, as assessed by western blot analyses of fractionated testicular lysates. |
2003-07-22 |
2023-08-12 |
mouse |
| I A McNeish, S Bell, T McKay, T Tenev, M Marani, N R Lemoin. Expression of Smac/DIABLO in ovarian carcinoma cells induces apoptosis via a caspase-9-mediated pathway. Experimental cell research. vol 286. issue 2. 2003-07-11. PMID:12749848. |
western blot analysis confirms that smac-induced apoptosis proceeds via a pathway mediated primarily by caspase-9 that can be inhibited by zlehd-fmk and overexpression of the x-linked inhibitor of apoptosis protein (xiap). |
2003-07-11 |
2023-08-12 |
Not clear |
| Minoru Satoh, Naoki Kashihara, Sohachi Fujimoto, Hideyuki Horike, Takehiko Tokura, Tamehachi Namikoshi, Tamaki Sasaki, Hirofumi Makin. A novel free radical scavenger, edarabone, protects against cisplatin-induced acute renal damage in vitro and in vivo. The Journal of pharmacology and experimental therapeutics. vol 305. issue 3. 2003-07-07. PMID:12649298. |
cisplatin resulted in renal dysfunction, renal tubular damage, mitochondrial damage (assayed by histochemical staining for respiratory chain complex iv), renal protein oxidation (examined by western blot analysis using a specific antibody for carbonyl group-containing proteins), and tubular apoptosis (determined by terminal deoxynucleotidyl transferase-mediated dutp nick end-labeling staining). |
2003-07-07 |
2023-08-12 |
rat |
| Fuzhong Qin, Junya Shite, Weike Mao, Chang-seng Lian. Selegiline attenuates cardiac oxidative stress and apoptosis in heart failure: association with improvement of cardiac function. European journal of pharmacology. vol 461. issue 2-3. 2003-05-23. PMID:12586210. |
after 8 weeks of treatment, animals were studied for arterial norepinephrine and left ventricular systolic function (fractional shortening and dp/dt), and were then sacrificed for measuring the stable oxidative product of myocardial mitochondrial dna (mtdna) 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxo-dg), myocyte apoptosis by monoclonal antibody to single stranded dna, and bcl-2 and bax protein expression by western blot and immunohistochemistry. |
2003-05-23 |
2023-08-12 |
Not clear |
| Thomas C Chen, Susan Su, David Fry, Leonard Liebe. Combination therapy with irinotecan and protein kinase C inhibitors in malignant glioma. Cancer. vol 97. issue 9 Suppl. 2003-05-16. PMID:12712458. |
the terminal deoxynucleotidyl transferase-mediated dutp nick-end labeling(tunel) assay was used to determine apoptosis as the mechanism of cytotoxicity; alterations in bcl-2 and bax expression were determined using western blot analysis. |
2003-05-16 |
2023-08-12 |
Not clear |
| Seyed Mohamed Ali, Malini Oliv. Efficacy of hypocrellin pharmacokinetics in phototherapy. International journal of oncology. vol 21. issue 6. 2003-05-14. PMID:12429972. |
in addition, the present study employed both immunocytochemical and western blot techniques to explore the photo-induced expression of apoptosis related proteins in npc as well as other human carcinoma cells. |
2003-05-14 |
2023-08-12 |
human |
| L T McPhail, J L Vanderluit, C B McBride, L W Oschipok, S J Crocker, D Xu, C S Thompson, P Liston, M Holcik, G S Robertson, W Tetzlaf. Endogenous expression of inhibitor of apoptosis proteins in facial motoneurons of neonatal and adult rats following axotomy. Neuroscience. vol 117. issue 3. 2003-05-05. PMID:12617963. |
furthermore, immunohistochemistry and western blot for neuronal apoptosis inhibitory protein revealed, in contrast to the observed increase in neuronal apoptosis inhibitory protein mrna, a decline in the expression of neuronal apoptosis inhibitory protein following axotomy in the adult, whereas no change in neuronal apoptosis inhibitory protein was detected in neonatal facial motoneurons. |
2003-05-05 |
2023-08-12 |
rat |
| L T McPhail, J L Vanderluit, C B McBride, L W Oschipok, S J Crocker, D Xu, C S Thompson, P Liston, M Holcik, G S Robertson, W Tetzlaf. Endogenous expression of inhibitor of apoptosis proteins in facial motoneurons of neonatal and adult rats following axotomy. Neuroscience. vol 117. issue 3. 2003-05-05. PMID:12617963. |
x-linked inhibitory apoptosis protein, as analyzed by immunohistochemistry and western blot, remained unchanged by axotomy in neonatal motoneurons and adult motoneurons. |
2003-05-05 |
2023-08-12 |
rat |
| E Chatzaki, E Kouimtzoglou, A N Margioris, A Gravani. Transforming growth factor beta1 exerts an autocrine regulatory effect on human endometrial stromal cell apoptosis, involving the FasL and Bcl-2 apoptotic pathways. Molecular human reproduction. vol 9. issue 2. 2003-04-29. PMID:12569178. |
we have found the following: (i) tgfbeta1 induced apoptosis of stromal cells in a time- and dose-dependent manner; (ii) blockade of tgfbeta1's autocrine/paracrine effect by tgfbeta1-neutralizing antibodies diminished the basal rate of stromal cell apoptosis; (iii) semi-quantitative western blot analysis showed that tgfbeta1 caused a rapid but transient elevation of the pro-apoptotic fasl protein, without affecting the levels of fas receptor; (iv) tgfbeta1 increased the levels of the anti-apoptotic bcl-2 and bcl-xl proteins, while having no significant effects on the pro-apoptotic proteins bax and bak, suggesting the activation of a transient survival mechanism activated in stromal cells as a parallel rescue response to the apoptosis-inducing fasl protein. |
2003-04-29 |
2023-08-12 |
human |
| Selwyn M Vickers, Zhi-Qiang Huang, LeeAnn MacMillan-Crow, Jessica S Greendorfer, John A Thompso. Ligand activation of alternatively spliced fibroblast growth factor receptor-1 modulates pancreatic adenocarcinoma cell malignancy. Journal of gastrointestinal surgery : official journal of the Society for Surgery of the Alimentary Tract. vol 6. issue 4. 2003-04-28. PMID:12127120. |
cells were evaluated for growth inhibition, apoptosis (propidium iodide staining and flow cytometry, caspase 3 activation) and for bcl-x(l)/bax expression (by western blot analysis). |
2003-04-28 |
2023-08-12 |
mouse |
| Marcella Flinterman, Joop Gäken, Farzin Farzaneh, Mahvash Tavassol. E1A-mediated suppression of EGFR expression and induction of apoptosis in head and neck squamous carcinoma cell lines. Oncogene. vol 22. issue 13. 2003-04-23. PMID:12673202. |
cell cycle and western blot analysis show e1a-mediated induction of apoptosis in all cell lines examined. |
2003-04-23 |
2023-08-12 |
human |
| Tetsuji Kakegawa, Hirohiko Ise, Nobuhiro Sugihara, Toshio Nikaido, Naoki Negishi, Toshihiro Akaike, Eiji Tanak. Soluble asialoglycoprotein receptors reflect the apoptosis of hepatocytes. Cell transplantation. vol 11. issue 5. 2003-04-18. PMID:12382666. |
in the present study, in order to investigate the correlation between apoptosis of hepatocytes and soluble agpr, mouse soluble agprs were detected using sds-page and western blot analysis was conducted using anti-extracellular mouse hepatic lectin-1 (ex-mhl-1) antiserum (polyclonal rabbit serum). |
2003-04-18 |
2023-08-12 |
human |
| W Keung Leung, Qiang Wu, Pauline M Hannam, Barry C McBride, Veli-Jukka Uitt. Treponema denticola may stimulate both epithelial proliferation and apoptosis through MAP kinase signal pathways. Journal of periodontal research. vol 37. issue 6. 2003-04-15. PMID:12472839. |
western blot analysis using map kinase phosphospecific antibodies showed that t. denticola strongly but transiently activated erk1 and erk2, signals mediating cell proliferation, and jnk and p38, kinases mediating apoptosis. |
2003-04-15 |
2023-08-12 |
human |
| Junichi Asakuma, Makoto Sumitomo, Takako Asano, Tomohiko Asano, Masamichi Hayakaw. Selective Akt inactivation and tumor necrosis actor-related apoptosis-inducing ligand sensitization of renal cancer cells by low concentrations of paclitaxel. Cancer research. vol 63. issue 6. 2003-04-11. PMID:12649200. |
apoptosis assays and western blot analyses revealed that apoptosis resistance to trail correlates well with the level of akt phosphorylation at ser 473 rather than protein expression levels of trail receptors, dr4 and dr5. |
2003-04-11 |
2023-08-12 |
human |