| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| J H Yang, F Feng, H Qian, H Chen. Chemosensitization of breast carcinoma cells with the use of bcl-2 antisense oligodeoxynucleotide. Breast (Edinburgh, Scotland). vol 13. issue 3. 2004-07-30. PMID:15177426. |
changes in expression of the bcl-2 gene were examined by western blot; cell growths were detected by mtt assay, and apoptosis rates were detected by flow cytometry (fcm). |
2004-07-30 |
2023-08-12 |
Not clear |
| Hedy H Ginzberg, Patrick T Shannon, Tomoko Suzuki, Ouyang Hong, Eric Vachon, Theo Moraes, Maria Teresa Herrera Abreu, Vera Cherepanov, Xiaomin Wang, Chung-Wai Chow, Gregory P Downe. Leukocyte elastase induces epithelial apoptosis: role of mitochondial permeability changes and Akt. American journal of physiology. Gastrointestinal and liver physiology. vol 287. issue 1. 2004-07-23. PMID:15194561. |
treatment with elastase resulted in decreased mitochondrial membrane potential, release of cytochrome c to the cytosol, and cleavage of caspases-9 and -3 as determined by western blot analysis, implicating altered mitochondrial membrane permeability as a primary mechanism for elastase-induced apoptosis. |
2004-07-23 |
2023-08-12 |
Not clear |
| Yan Zhao, Kun Wu, Yinghui Yu, Guichang L. [Roles of ERK1/2 MAPK in vitamin E succinate-induced apoptosis in human gastric cancer SGC-7901 cells]. Wei sheng yan jiu = Journal of hygiene research. vol 32. issue 6. 2004-07-22. PMID:14963907. |
in order to investigate the roles of erk1/2 mitogen-activated protein kinase in vitamin e succinate (ves)-induced apoptosis in human gastric cancer sgc-7901 cells, apoptosis was observed by dapi staining and the phosphorylation of erk1/2 by ves at different doses and different time points was measured by western blot. |
2004-07-22 |
2023-08-12 |
human |
| Peng Wei, Shi-Xin Tao, Xue-Sen Zhang, Zhao-Yuan Hu, Liu Yi-Xu. [Effect of RU486 on apoptosis and p53 expression at the boundary of fetal-maternal interface of rhesus monkey (Macaca mulatta)]. Sheng li xue bao : [Acta physiologica Sinica]. vol 56. issue 1. 2004-07-22. PMID:14985831. |
to study the effect of ru486 on apoptosis and expression of p53 at the fetal-maternal interface, the location of apoptotic cells and expression of p53 were examined using in situ 3'-end labeling method, immunohistochemistry and western blot assay at the fetal-maternal interface of normal and ru486 treated rhesus monkey. |
2004-07-22 |
2023-08-12 |
monkey |
| Maria A Mariggiò, Sergio Cafaggi, Massimo Ottone, Brunella Parodi, Maria O Vannozzi, Vaclav Mandys, Maurizio Vial. Inhibition of cell growth, induction of apoptosis and mechanism of action of the novel platinum compound cis-diaminechloro-[2-(diethylamino) ethyl 4-amino-benzoate, N(4)]-chloride platinum (II) monohydrochloride monohydrate. Investigational new drugs. vol 22. issue 1. 2004-07-19. PMID:14707490. |
some of them were also evaluated for the induction of apoptosis by 4'-6-diamidine-2'-phenylindole (dapi) staining, western blot of p53 protein and agarose gel electrophoresis of ladder dna. |
2004-07-19 |
2023-08-12 |
Not clear |
| Lan Sun, Wen-Li Liu, Han-Ying Sun, Yin-Li Zhou, Hui-Zhen Xu, Wu L. [Effect of Ligustrazine on the expression of vascular endothelial growth factor in bone marrow stromal cells of radiation injured mice]. Zhongguo shi yan xue ye xue za zhi. vol 12. issue 1. 2004-06-15. PMID:14989777. |
to evaluate the effect of ligustrazine on the expression of vegf in bone marrow stromal cells (bmscs) of radiation injured mice and to explore the effect of vegf on the recovery of hematopoiesis and the mechanism of signal transduction, the protein expression of vegf, focal adhesion kinase (fak) and mitogen-activated protein kinase (mapk) in bmscs were assayed by western blot, the cell cycle and apoptosis rate of bmscs were tested by flow cytometry. |
2004-06-15 |
2023-08-12 |
mouse |
| Juan Xiao, Ping Zou, Shi-Ang Huang, Zhong-Bo Hu, Ling-Bo Liu, Yong Yo. [Role of apoptosis in cryoinjury of cord blood hematopoietic stem/progenitor cells and its mechanism]. Zhongguo shi yan xue ye xue za zhi. vol 12. issue 1. 2004-06-15. PMID:14989778. |
to investigate the role and mechanism of apoptosis in cryoinjury of cord blood hematopoietic stem/progenitor cells, apoptosis of cd34(+) cells, mitochondrial membrane potential (mmp) and fas antigen expression were detected by flow cytometry (fcm), the bcl-2 protein expression was detected by immunohistochemistry, caspase-3 expression was determined by western blot and caspase-3 activity analysis, colony-forming units (cfu) was performed by semi-solid methylcellulose culture. |
2004-06-15 |
2023-08-12 |
Not clear |
| A Duvoix, F Morceau, M Schnekenburger, S Delhalle, M M Galteau, M Dicato, M Diederic. Curcumin-induced cell death in two leukemia cell lines: K562 and Jurkat. Annals of the New York Academy of Sciences. vol 1010. 2004-06-15. PMID:15033758. |
cell death was induced in both cell lines, and apoptosis pathways were investigated by western blot analysis. |
2004-06-15 |
2023-08-12 |
Not clear |
| Jolanta Dorszewska, Zofia Adamczewska-Goncerzewicz, Józef Szczec. Apoptotic proteins in the course of aging of central nervous system in the rat. Respiratory physiology & neurobiology. vol 139. issue 2. 2004-05-28. PMID:15122998. |
studies were performed on the level of cells with damaged dna (tunel), the level of protein engaged in dna repair (parp) and the level of proteins indicating the extent of apoptosis (bax:bcl-2) (western blot). |
2004-05-28 |
2023-08-12 |
rat |
| Daniela Verzola, Maria Bianca Bertolotto, Barbara Villaggio, Luciano Ottonello, Franco Dallegri, Francesca Salvatore, Valeria Berruti, Maria Teresa Gandolfo, Giacomo Garibotto, Giacomo Deferrar. Oxidative stress mediates apoptotic changes induced by hyperglycemia in human tubular kidney cells. Journal of the American Society of Nephrology : JASN. vol 15 Suppl 1. 2004-05-25. PMID:14684680. |
apoptosis (annexin v binding), ros formation (fluorescence probe dichlorofluorescin diacetate and facscan flow cytometry), and x chromosome-linked protein (xiap; western blot) were studied in hk-2 cells grown in a medium containing normal (ng) or high glucose (hg) concentrations (5.5 or 30 mm, respectively) for 18 to 48 h. hg promoted an increase (65% at 18 h and 73% at 24 h; p < 0.05 versus ng) in intracellular ros generation. |
2004-05-25 |
2023-08-12 |
human |
| D Uberti, T Carsana, S Francisconi, G Ferrari Toninelli, P L Canonico, M Mem. A novel mechanism for pergolide-induced neuroprotection: inhibition of NF-kappaB nuclear translocation. Biochemical pharmacology. vol 67. issue 9. 2004-05-25. PMID:15081873. |
based on western blot analysis and immunocytochemistry, pergolide was found to prevent h(2)o(2)-induced apoptosis by inhibiting nf-kappab nuclear translocation and activation of p53 signalling pathway. |
2004-05-25 |
2023-08-12 |
Not clear |
| Wen L Shih, Hsiao W Hsu, Ming H Liao, Long H Lee, Hung J Li. Avian reovirus sigmaC protein induces apoptosis in cultured cells. Virology. vol 321. issue 1. 2004-05-12. PMID:15033566. |
five kinds of assays for apoptosis were used in analyzing arv-infected bhk-21 and vero cells: (1) assay for dna ladders, (2) elisa detection of cytoplasmic histone-associated dna fragments, (3) nuclear staining with acridine orange, (4) western blot, northern blot, and immunofluorescent assay (ifa), and (5) flow cytometric analysis. |
2004-05-12 |
2023-08-12 |
Not clear |
| Stephan Schmidt, Edin Moric, Martina Schmidt, Magdalena Sastre, Douglas L Feinstein, Michael T Henek. Anti-inflammatory and antiproliferative actions of PPAR-gamma agonists on T lymphocytes derived from MS patients. Journal of leukocyte biology. vol 75. issue 3. 2004-04-23. PMID:14657213. |
electron microscopy and western blot analysis revealed dna condensation and down-regulation of bcl-2, suggesting the induction of apoptosis in activated t lymphocytes. |
2004-04-23 |
2023-08-12 |
human |
| Shuqian Liu, Hui Huang, Xin Lu, Miroslaw Golinski, Sebastien Comesse, David Watt, Robert B Grossman, Jeffrey A Mosco. Down-regulation of thiamine transporter THTR2 gene expression in breast cancer and its association with resistance to apoptosis. Molecular cancer research : MCR. vol 1. issue 9. 2004-04-20. PMID:12861052. |
tunel assays demonstrate an increase in apoptosis in thtr2-transfected cells exposed to doxorubicin and radiation, and western blot analysis suggests that apoptosis associated with these cytotoxic stresses is mediated at least in part by a caspase-3-dependent pathway. |
2004-04-20 |
2023-08-12 |
human |
| Yong Li, Paul J Cozzi, Chang F Qu, David Y Zhang, Syed M Abbas Rizvi, Chand Raja, Barry J Alle. Cytotoxicity of human prostate cancer cell lines in vitro and induction of apoptosis using 213Bi-Herceptin alpha-conjugate. Cancer letters. vol 205. issue 2. 2004-04-12. PMID:15036648. |
we used immunocytochemistry, flow cytometry and western blot analysis to examine the expression of her-2 in a panel of established human cap cell lines, used the mts assay to evaluate the cytotoxicity of 213bi-herceptin on these cell lines and the tunel assay to document apoptosis. |
2004-04-12 |
2023-08-12 |
human |
| K Erkkilä, L Suomalainen, M Wikström, M Parvinen, L Dunke. Chemical anoxia delays germ cell apoptosis in the human testis. Biology of reproduction. vol 69. issue 2. 2004-04-06. PMID:12700196. |
we investigated the levels of ans during human testicular apoptosis (analyzed by hplc) and the role of chemical anoxia in germ cell death (detected by southern blot analysis of dna fragmentation, in situ end labeling of dna, and electron microscopy). |
2004-04-06 |
2023-08-12 |
human |
| Kerstin Jönsson-Videsäter, Linda Björkhem-Bergman, Akter Hossain, Anita Söderberg, Lennart C Eriksson, Christer Paul, Anders Rosén, Mikael Björnsted. Selenite-induced apoptosis in doxorubicin-resistant cells and effects on the thioredoxin system. Biochemical pharmacology. vol 67. issue 3. 2004-04-05. PMID:15037203. |
the apoptosis was caspase-3 independent as revealed by western blot analysis, activity measurement and by using caspase inhibitors. |
2004-04-05 |
2023-08-12 |
human |
| Sonya Trombino, Alfredo Cesario, Stefano Margaritora, PierLuigi Granone, Giovanni Motta, Carla Falugi, Patrizia Russ. Alpha7-nicotinic acetylcholine receptors affect growth regulation of human mesothelioma cells: role of mitogen-activated protein kinase pathway. Cancer research. vol 64. issue 1. 2004-04-01. PMID:14729617. |
thus, these data demonstrate that: (a) human mesothelioma cells and human biopsies of mesothelioma as well as of normal pleural mesothelial cells express functionally alpha-7 nicotinic acethlycholine receptors, evaluated by alpha-bungarotoxin-fitc binding, receptor binding assay, western blot, and reverse transcription-pcr; (b) choline acetyltransferase immunostaining is present in mesothelioma cells; (c) mesothelioma cell growth is modulated by the cholinergic system in which agonists (i.e., nicotine) has a proliferative effect, and antagonists (i.e., curare) has an inhibitory effect, evaluated by cell cloning, dna synthesis and cell cycle; (d) nicotine induces ca(+2) influx, evaluated by [(45)ca(2+)] uptake, and consequently activation of mitogen-activated protein kinase pathway (extracellular signal-regulated kinase and p90(rsk) phosphorylation), evaluated by western blot; and (e) apoptosis mechanisms in mesothelioma cells are under the control of the cholinergic system (nicotine antiapoptotic via induction of nuclear factor-kappab complexes and phosphorylation of bad at ser(112); curare proapoptotic via g(0)-g(1) arrest p21(waf-1) dependent but p53 independent). |
2004-04-01 |
2023-08-12 |
human |
| Antonina S Rait, Kathleen F Pirollo, David Ulick, Kevin Cullen, Esther H Chan. HER-2-targeted antisense oligonucleotide results in sensitization of head and neck cancer cells to chemotherapeutic agents. Annals of the New York Academy of Sciences. vol 1002. 2004-03-24. PMID:14751825. |
cell survival assays and western blot analysis data demonstrated that folate-liposome mediated as her-2 oligonucleotide treatment inhibited cell growth and her-2 expression, and induced apoptosis in scc-25cp cells. |
2004-03-24 |
2023-08-12 |
human |
| Yong J Lee, Jae J Song, Jin H Kim, Hyeong-Reh Choi Kim, Young K Son. Low extracellular pH augments TRAIL-induced apoptotic death through the mitochondria-mediated caspase signal transduction pathway. Experimental cell research. vol 293. issue 1. 2004-03-11. PMID:14729063. |
western blot analysis also shows that the low extracellular ph-enhanced trail cytotoxicity does not involve modulation of the levels of trail receptors (dr4, dr5, and dcr2), flip, inhibitor of apoptosis (iap), and bcl-2. |
2004-03-11 |
2023-08-12 |
human |