| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Ki M Kim, Yong J Le. Amiloride augments TRAIL-induced apoptotic death by inhibiting phosphorylation of kinases and phosphatases associated with the P13K-Akt pathway. Oncogene. vol 24. issue 3. 2005-03-01. PMID:15558024. |
western blot analysis showed that combined treatment with trail and amiloride did not change the levels of trail receptors (death receptor (dr)4, dr5, and dcr2 (decoy recepter 2) or antiapoptotic proteins (flice-inhibitory protein (flip), inhibitor of apoptosis (iap), and bcl-2). |
2005-03-01 |
2023-08-12 |
human |
| Stephanie K Y Chow, Judy Y W Chan, K P Fun. Inhibition of cell proliferation and the action mechanisms of arsenic trioxide (As2O3) on human breast cancer cells. Journal of cellular biochemistry. vol 93. issue 1. 2005-02-22. PMID:15352174. |
further studies by western blot analysis indicated that as(2)o(3) regulated apoptosis and the expression of cell-cycle-related proteins as it upregulated p53 protein level and downregulated bcl-2 protein level. |
2005-02-22 |
2023-08-12 |
human |
| Ge Li, Dong-Hee Kim, Tae-Dong Kim, Byoung-Jeon Park, Hae-Duck Park, Jong-Il Park, Min-Kyun Na, Hwan-Chul Kim, Nam-Doo Hong, Kyu Lim, Byung-Doo Hwang, Wan-Hee Yoo. Protein-bound polysaccharide from Phellinus linteus induces G2/M phase arrest and apoptosis in SW480 human colon cancer cells. Cancer letters. vol 216. issue 2. 2005-02-18. PMID:15533593. |
western blot analysis suggested that pl-induced apoptosis and growth inhibition were associated with decrease in bcl-2, increase of the release of cytochrome c, and reduced expression of cyclin b1. |
2005-02-18 |
2023-08-12 |
human |
| R Misseri, D R Meldrum, C A Dinarello, P Dagher, K L Hile, R C Rink, K K Meldru. TNF-alpha mediates obstruction-induced renal tubular cell apoptosis and proapoptotic signaling. American journal of physiology. Renal physiology. vol 288. issue 2. 2005-02-09. PMID:15507546. |
the kidneys were harvested 1, 3, or 7 days postoperatively, and tissue samples were subsequently analyzed for tnf-alpha (elisa, rt-pcr), fas ligand (rt-pcr), apoptosis (tunel, elisa), and caspase 8 and 3 activity (western blot). |
2005-02-09 |
2023-08-12 |
rat |
| Leslie P Cousens, Frederick A Goulette, James W Darnowsk. JAK-mediated signaling inhibits Fas ligand-induced apoptosis independent of de novo protein synthesis. Journal of immunology (Baltimore, Md. : 1950). vol 174. issue 1. 2005-01-27. PMID:15611255. |
in a concentration-dependent manner, fasl induced apoptosis, as shown by western blot analysis of procaspase 8 and poly(adp-ribose) polymerase cleavage after 3-h exposure and by cytofluorometric analysis of sub-g(0)-g(1) cellular dna content after 24-h exposure. |
2005-01-27 |
2023-08-12 |
human |
| Jacqueline Fung, Matthew J Hoffmann, David D Kim, Robert Snyde. Inhibition of topoisomerase II in 32D.3(G) cells by hydroquinone is associated with cell death. Journal of applied toxicology : JAT. vol 24. issue 3. 2004-12-02. PMID:15211611. |
progressive inability to detect topo ii protein by western blot analysis as hydroquinone concentrations were increased suggested that either association of the probe with the enzyme was inhibited by hydroquinone or there was degradation of the protein as a function of hydroquinone-induced apoptosis. |
2004-12-02 |
2023-08-12 |
human |
| Malisetty V Swamy, Indranie Cooma, Jagan M R Patlolla, Barbara Simi, Bandaru S Reddy, Chinthalapally V Ra. Modulation of cyclooxygenase-2 activities by the combined action of celecoxib and decosahexaenoic acid: novel strategies for colon cancer prevention and treatment. Molecular cancer therapeutics. vol 3. issue 2. 2004-11-22. PMID:14985462. |
hca-7 cells exposed to various subtoxic doses of celecoxib, dha, or combinations of both were analyzed for inhibition of cell proliferation by trypan blue exclusion and proliferating cell nuclear antigen methods, induction of apoptosis by 4',6-diamidino-2-phenylindole method, and cox-2 by reverse transcription-pcr and western blot analysis. |
2004-11-22 |
2023-08-12 |
human |
| C M Payne, C N Waltmire, C Crowley, C L Crowley-Weber, K Dvorakova, H Bernstein, C Bernstein, H Holubec, H Garewa. Caspase-6 mediated cleavage of guanylate cyclase alpha 1 during deoxycholate-induced apoptosis: protective role of the nitric oxide signaling module. Cell biology and toxicology. vol 19. issue 6. 2004-11-12. PMID:15015762. |
a novel finding from this study is the caspase-6 mediated cleavage of guanylate cyclase alpha 1 during deoxycholate-induced apoptosis of deoxycholate-sensitive hct-116sa cells and the absence of guanylate cyclase alpha 1 cleavage in deoxycholate-treated hct-116r resistant cells using western blot analyses. |
2004-11-12 |
2023-08-12 |
Not clear |
| Tong Wu, Jing Leng, Chang Han, Anthony Jake Demetri. The cyclooxygenase-2 inhibitor celecoxib blocks phosphorylation of Akt and induces apoptosis in human cholangiocarcinoma cells. Molecular cancer therapeutics. vol 3. issue 3. 2004-11-09. PMID:15026550. |
fluorescence microscopy, western blot, and caspase activity assays demonstrated that celecoxib induced morphological features of apoptosis, activation of caspase-9 and caspase-3, and release of cytochrome c. the celecoxib-induced cell death was significantly blocked by n-benzyloxy-carbonyl-val-ala-asp-fluoromethylketone, a wide-spectrum caspase inhibitor. |
2004-11-09 |
2023-08-12 |
human |
| Paul Mrass, Michael Rendl, Michael Mildner, Florian Gruber, Barbara Lengauer, Claudia Ballaun, Leopold Eckhart, Erwin Tschachle. Retinoic acid increases the expression of p53 and proapoptotic caspases and sensitizes keratinocytes to apoptosis: a possible explanation for tumor preventive action of retinoids. Cancer research. vol 64. issue 18. 2004-11-04. PMID:15374966. |
analysis by real-time pcr and western blot revealed that atra treatment strongly increased the mrna and protein expression of p53 and caspase-3, -6, -7, and -9, which are key regulators of apoptosis. |
2004-11-04 |
2023-08-12 |
Not clear |
| Rajendra Persad, Chen Liu, Tsung-Teh Wu, Patrick S Houlihan, Stanley R Hamilton, Anna M Diehl, Asif Rashi. Overexpression of caspase-3 in hepatocellular carcinomas. Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc. vol 17. issue 7. 2004-11-02. PMID:15098015. |
therefore, we studied its expression in four hepatoma cell lines and 22 hccs by western blot, and correlated the findings with in vitro caspase-3 activity and apoptosis. |
2004-11-02 |
2023-08-12 |
human |
| Michael Stanglmaier, Simone Reis, Michael Halle. Rituximab and alemtuzumab induce a nonclassic, caspase-independent apoptotic pathway in B-lymphoid cell lines and in chronic lymphocytic leukemia cells. Annals of hematology. vol 83. issue 10. 2004-11-02. PMID:15309525. |
cell morphology and western blot analyses revealed that the antibody-induced cell death lacked some typical features of apoptosis such as chromatin condensation or poly-adp-ribose polymerase (parp) cleavage. |
2004-11-02 |
2023-08-12 |
human |
| Daniel Nowak, Simone Boehrer, Angela Brieger, Soo-Zin Kim, Simone Schaaf, Dieter Hoelzer, Paris S Mitrou, Eckhart Weidmann, Kai Uwe Cho. Upon drug-induced apoptosis in lymphoma cells X-linked inhibitor of apoptosis (XIAP) translocates from the cytosol to the nucleus. Leukemia & lymphoma. vol 45. issue 7. 2004-11-01. PMID:15359644. |
the influence of drug-induced apoptosis on localization of iaps was investigated using immunofluorescence microscopy as well as western blot analysis. |
2004-11-01 |
2023-08-12 |
Not clear |
| Jen-Hung Yang, Chi-Chung Chou, Ya-Wen Cheng, Lee-Yan Sheen, Ming-Chi Chou, Hsin-Su Yu, Yau-Huei Wei, Jing-Gung Chun. Effects of glycolic acid on the induction of apoptosis via caspase-3 activation in human leukemia cell line (HL-60). Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association. vol 42. issue 11. 2004-10-21. PMID:15350675. |
the morphological changes, cell viability, apoptosis induction, and caspase-3 activity were measured by phase microscopy, flow cytometry, and western blot analysis. |
2004-10-21 |
2023-08-12 |
human |
| Chye Sun Ong, Evelyn Tran, T T Tuyen Nguyen, Choon Kiat Ong, Siew Kuen Lee, Jia Jing Lee, Chee Pang Ng, Caine Leong, Hung Huyn. Quercetin-induced growth inhibition and cell death in nasopharyngeal carcinoma cells are associated with increase in Bad and hypophosphorylated retinoblastoma expressions. Oncology reports. vol 11. issue 3. 2004-10-07. PMID:14767529. |
western blot experiments confirmed that cytotoxic killing of hk1 and cne2 by quercetin was mediated by the up-regulation of pro-apoptotic protein bad, caspase-3 and -7, resulting in cell death by apoptosis. |
2004-10-07 |
2023-08-12 |
Not clear |
| H F Lu, C C Sue, C S Yu, S C Chen, G W Chen, J G Chun. Diallyl disulfide (DADS) induced apoptosis undergo caspase-3 activity in human bladder cancer T24 cells. Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association. vol 42. issue 10. 2004-09-20. PMID:15304301. |
apoptosis induction, cell viability, cell cycle arrest, caspases-3, -9 activity and gene expression were measured to determine their variation by flow cytometric assay, western blot, and determination of caspase-3 activity, pcr and cdna microarray. |
2004-09-20 |
2023-08-12 |
human |
| Yu-Fang Kuang, Yue-Hwa Che. Induction of apoptosis in a non-small cell human lung cancer cell line by isothiocyanates is associated with P53 and P21. Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association. vol 42. issue 10. 2004-09-20. PMID:15354323. |
furthermore, western blot analyses demonstrated that increased expression of p53 and p21 proteins, but not bax protein, were associated with peitc- and bitc-induced apoptosis. |
2004-09-20 |
2023-08-12 |
human |
| S Schweyer, A Soruri, O Meschter, A Heintze, F Zschunke, N Miosge, P Thelen, T Schlott, H J Radzun, A Fayyaz. Cisplatin-induced apoptosis in human malignant testicular germ cell lines depends on MEK/ERK activation. British journal of cancer. vol 91. issue 3. 2004-08-31. PMID:15266324. |
applying cdna macroarray, semiquantitative rt-pcr and western blot analyses, blocking experiments, caspase activity assays, and morphological methods, we sought here to define the p53-independent pathway(s) involved in the cddp-induced apoptosis. |
2004-08-31 |
2023-08-12 |
human |
| Marieke H Schoemaker, Laura Conde de la Rosa, Manon Buist-Homan, Titia E Vrenken, Rick Havinga, Klaas Poelstra, Hidde J Haisma, Peter L M Jansen, Han Moshag. Tauroursodeoxycholic acid protects rat hepatocytes from bile acid-induced apoptosis via activation of survival pathways. Hepatology (Baltimore, Md.). vol 39. issue 6. 2004-08-30. PMID:15185297. |
transcription was blocked by actinomycin-d. apoptosis was determined by measuring caspase-3, -9, and -8 activity using fluorimetric enzyme detection, western blot analysis, immunocytochemistry, and nuclear morphological analysis. |
2004-08-30 |
2023-08-12 |
rat |
| Yeung-Leung Cheng, Wen-Liang Chang, Shih-Chun Lee, Yau-Gye Liu, Cheng-Jueng Chen, Shinn-Zong Lin, Nu-Man Tsai, Dah-Shyong Yu, Chung-Yang Yen, Horng-Jyh Har. Acetone extract of Angelica sinensis inhibits proliferation of human cancer cells via inducing cell cycle arrest and apoptosis. Life sciences. vol 75. issue 13. 2004-08-25. PMID:15261763. |
the western blot indicated the ae-as-triggered apoptosis is mediated via suppression of bcl-2 oncoprotein expression rather than p53 or bax. |
2004-08-25 |
2023-08-12 |
human |