| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Gisela Gutiérrez, Criselda Mendoza, Luis F Montaño, Rebeca López-Marur. Ceramide induces early and late apoptosis in human papilloma virus+ cervical cancer cells by inhibiting reactive oxygen species decay, diminishing the intracellular concentration of glutathione and increasing nuclear factor-kappaB translocation. Anti-cancer drugs. vol 18. issue 2. 2007-03-06. PMID:17159601. |
reactive oxygen species production was determined by fluorescent 2,7-dichlorofluorescein, nitrite concentration by the griess reaction (as an indirect measure of nitric oxide production), mitochondrial membrane potential by staining with rh123, reduced-glutathione concentration by high-pressure liquid chromatography, nuclear factor-kappab translocation by electrophoretic mobility shift assay, inhibitory protein of nuclear factor-kappab expression by western blot and cell death by a poly-caspases fluorochrome-labeled inhibitors of caspases apoptosis assay. |
2007-03-06 |
2023-08-12 |
human |
| Puja Chauhan, Ajit Sodhi, Shikha Taran. Cisplatin-treated murine peritoneal macrophages induce apoptosis in L929 cells: role of Fas-Fas ligand and tumor necrosis factor-tumor necrosis factor receptor 1. Anti-cancer drugs. vol 18. issue 2. 2007-03-06. PMID:17159605. |
the induction of apoptosis in l929 cells was investigated by dna fragmentation, annexin v staining and western blot analysis of bax, bcl-2, bid, cytochrome c, poly(adp ribose) polymerase, cad, caspase-8 and caspase-3. |
2007-03-06 |
2023-08-12 |
Not clear |
| Ji-Young Kim, Myoung-Hee Ahn, Hye-Sun Jun, Jai-Won Jung, Jae-Sook Ryu, Duk-Young Mi. Toxoplasma gondii inhibits apoptosis in infected cells by caspase inactivation and NF-kappaB activation. Yonsei medical journal. vol 47. issue 6. 2007-03-05. PMID:17191317. |
analysis of emsa and western blot data suggests that activation of transcription factor nf-kappab may be involved in the blockade of apoptosis by t. gondii. |
2007-03-05 |
2023-08-12 |
mouse |
| I Solaroglu, T Tsubokawa, J Cahill, J H Zhan. Anti-apoptotic effect of granulocyte-colony stimulating factor after focal cerebral ischemia in the rat. Neuroscience. vol 143. issue 4. 2007-02-23. PMID:17084035. |
brains were harvested either 24 h, 72 h or 2 weeks after reperfusion for assays of infarct volume, immunohistological studies and western blot analysis for phosphorylated signal transducer and activator of transcription 3 (pstat3), pim-1, bcl-2, bax, cytochrome c, cellular inhibitor of apoptosis protein 2 (ciap2), and cleaved caspase-3 levels. |
2007-02-23 |
2023-08-12 |
rat |
| Manchao Zhang, Xueliang Fang, Hongpeng Liu, Shaomeng Wang, Dajun Yan. Blockade of AKT activation in prostate cancer cells with a small molecule inhibitor, 9-chloro-2-methylellipticinium acetate (CMEP). Biochemical pharmacology. vol 73. issue 1. 2007-02-22. PMID:16950208. |
the inhibitory effects of a potent and selective akt/bkb small molecule inhibitor, 9-chloro-2-methylellipticinium acetate (cmep), on the activation of akt, its antiproliferation and apoptosis-inducing effects in prostate cancer cell lines: du-145, pc-3, lncap, and cl-1, an androgen-independent lncap variant, and cl-1 xenograft mouse model were assessed by western blot analysis, kinase assay, cell survival assay, and apoptosis assay in this report. |
2007-02-22 |
2023-08-12 |
mouse |
| Kazuya Matsumoto, Jun-Ichi Okano, Takakazu Nagahara, Yoshikazu Murawak. Apoptosis of liver cancer cells by vitamin K2 and enhancement by MEK inhibition. International journal of oncology. vol 29. issue 6. 2007-02-22. PMID:17088989. |
however, western blot analysis revealed that neither apoptosis induction by vk2 nor enhancement of apoptosis by u0126 was mediated by caspase activation. |
2007-02-22 |
2023-08-12 |
Not clear |
| Jordi Monfort, Natalia Garcia-Giralt, María J López-Armada, Joan C Monllau, Angeles Bonilla, Pere Benito, Francisco J Blanc. Decreased metalloproteinase production as a response to mechanical pressure in human cartilage: a mechanism for homeostatic regulation. Arthritis research & therapy. vol 8. issue 5. 2007-02-09. PMID:16972994. |
because human femoral heads are subjected to different loads at defined sites, both areas were obtained from the same hip and subsequently evaluated for differences in aggrecan, type ii collagen, mmp-1, and mmp-3 content (enzyme-linked immunosorbent assay) and gene expression (real-time polymerase chain reaction) and for chondrocyte apoptosis (flow cytometry, bcl-2 western blot, and mitochondrial membrane potential analysis). |
2007-02-09 |
2023-08-12 |
human |
| Ebru Gurisik, Kristina Warton, Donald K Martin, Stella M Valenzuel. An in vitro study of the effects of exposure to a GSM signal in two human cell lines: monocytic U937 and neuroblastoma SK-N-SH. Cell biology international. vol 30. issue 10. 2007-01-31. PMID:16877012. |
for the cell line sk-n-sh, effects were specifically assessed by gene microarray, followed by real-time pcr of the genes of interest, western blot analysis was used to measure heat shock protein levels, and flow cytometry to measure cell cycle distributions and apoptosis. |
2007-01-31 |
2023-08-12 |
human |
| Waneene C Dorsey, Paul B Tchounwou, Byron D For. Neuregulin 1-Beta cytoprotective role in AML 12 mouse hepatocytes exposed to pentachlorophenol. International journal of environmental research and public health. vol 3. issue 1. 2007-01-30. PMID:16823072. |
western blot analysis demonstrated the potential of pcp to induce oxidative stress and inflammatory response (c-fos), growth arrest and dna damage (gadd153), proteotoxic effects (hsp70), cell cycle arrest as consequence of dna damage (p53), mitogenic response (cyclin- d1), and apoptosis (caspase-3). |
2007-01-30 |
2023-08-12 |
mouse |
| Jianzhong Shentu, Bo Zhang, Lingling Fan, Qioajun He, Bo Yang, Zhi Che. Anti-proliferative activity of fenretinide in human hepatoma cells in vitro and in vivo. Anti-cancer drugs. vol 18. issue 1. 2007-01-30. PMID:17159502. |
western blot analysis further showed down-regulation of procaspase-3, x-linked inhibitor of apoptosis protein and poly(adp-ribose) polymerase cleavage in bel-7402 cells treated with 15 mumol/l fenretinide for 48 h. overexpression of p53 was observed in a time-dependent manner, along with a decrease in the bcl-2/bax ratio. |
2007-01-30 |
2023-08-12 |
mouse |
| María S Renna, Silvia G Correa, Carina Porporatto, Carlos M Figueredo, María P Aoki, María G Paraje, Claudia E Sotomayo. Hepatocellular apoptosis during Candida albicans colonization: involvement of TNF-alpha and infiltrating Fas-L positive lymphocytes. International immunology. vol 18. issue 12. 2007-01-22. PMID:17053043. |
we studied in situ apoptosis by 4',6-diamidino-2-phenylindole dihydrochloride and terminal deoxynucleotidyl transferase dutp nick-end labeling reactions, the levels of local tumor necrosis factor (tnf)-alpha mrna by reverse transcription-pcr and the fas/fas-l expression by immunohistochemistry and western blot. |
2007-01-22 |
2023-08-12 |
rat |
| Ling Geng, Kyle C Cuneo, Allie Fu, Tianxiang Tu, Peter W Atadja, Dennis E Hallaha. Histone deacetylase (HDAC) inhibitor LBH589 increases duration of gamma-H2AX foci and confines HDAC4 to the cytoplasm in irradiated non-small cell lung cancer. Cancer research. vol 66. issue 23. 2007-01-10. PMID:17145876. |
annexin-propidium iodide flow cytometry assays, cell morphology studies, and cleaved caspase-3 western blot analysis revealed a synergistic effect of lbh589 with ir in inducing apoptosis. |
2007-01-10 |
2023-08-12 |
human |
| H J Kwon, Y K Hong, K H Kim, C H Han, S H Cho, J S Choi, Byung-Woo Ki. Methanolic extract of Pterocarpus santalinus induces apoptosis in HeLa cells. Journal of ethnopharmacology. vol 105. issue 1-2. 2006-12-22. PMID:16326057. |
in western blot analysis, apoptosis in the hela cells was associated with the release of cytochrome c from mitochondria into the cytosol, activation of caspases-3, -8, -9 and proteolytic cleavage of parp. |
2006-12-22 |
2023-08-12 |
human |
| D L Bemis, J L Capodice, P Gorroochurn, A E Katz, R Buttya. Anti-prostate cancer activity of a beta-carboline alkaloid enriched extract from Rauwolfia vomitoria. International journal of oncology. vol 29. issue 5. 2006-12-22. PMID:17016636. |
western blot detection of parp cleavage and accumulation of cells containing sub-genomic dna indicated induction of apoptosis. |
2006-12-22 |
2023-08-12 |
mouse |
| Sandra Mueller, Marcus Schittenhelm, Friedemann Honecker, Elke Malenke, Kirsten Lauber, Sebastian Wesselborg, Joerg T Hartmann, Carsten Bokemeyer, Frank Maye. Cell-cycle progression and response of germ cell tumors to cisplatin in vitro. International journal of oncology. vol 29. issue 2. 2006-12-14. PMID:16820891. |
cell-cycle progression and induction of apoptosis were assessed by flow cytometry and western blot analysis of parp cleavage in the gct derived cell lines, nt2 and 2102 ep, and compared with the breast carcinoma cell line mcf-7. |
2006-12-14 |
2023-08-12 |
Not clear |
| Dong-Mei Chen, Xin Cai, Catherine L Kwik-Uribe, Rong Zeng, Xing-Zu Zh. Inhibitory effects of procyanidin B(2) dimer on lipid-laden macrophage formation. Journal of cardiovascular pharmacology. vol 48. issue 2. 2006-12-07. PMID:16954822. |
further reverse transcriptase-polymerase chain reaction and western blot analysis revealed that b(2) strongly inhibited arachidonic acid inflammatory reactions, apoptosis, and their coupled mitogen-activated protein kinase and nf-kappab pathways. |
2006-12-07 |
2023-08-12 |
Not clear |
| G M Rath, C Schneider, S Dedieu, B Rothhut, M Soula-Rothhut, C Ghoneim, B Sid, H Morjani, H El Btaouri, L Martin. The C-terminal CD47/IAP-binding domain of thrombospondin-1 prevents camptothecin- and doxorubicin-induced apoptosis in human thyroid carcinoma cells. Biochimica et biophysica acta. vol 1763. issue 10. 2006-12-06. PMID:16962673. |
on the other hand, drug-induced apoptosis determined by western blot analysis for parp cleavage and caspase-3 activity measurement, was significantly decreased in presence of exogenous tsp-1. |
2006-12-06 |
2023-08-12 |
human |
| Sandra Osbild, Laurent Brault, Eric Battaglia, Denyse Bagre. Resistance to cisplatin and adriamycin is associated with the inhibition of glutathione efflux in MCF-7-derived cells. Anticancer research. vol 26. issue 5A. 2006-11-30. PMID:17094488. |
cytotoxicity was evaluated by mtt assay, reactive oxygen species (ros), apoptosis and necrosis by flow cytometry, glutathione (gsh) by hplc, and bcl-2, bax and parp expression by western blot. |
2006-11-30 |
2023-08-12 |
human |
| Seon-Joo Park, In-Sook Ki. The role of p38 MAPK activation in auranofin-induced apoptosis of human promyelocytic leukaemia HL-60 cells. British journal of pharmacology. vol 146. issue 4. 2006-11-29. PMID:16086031. |
western blot analysis showed that af-induced apoptosis was accompanied by the activation of caspase-8, caspase-9, and caspase-3, and the release of cytochrome c from the mitochondria. |
2006-11-29 |
2023-08-12 |
human |
| Chris A Owen, Jamuna Selvakumaran, Ioan Notingher, Gavin Jell, Larry L Hench, Molly M Steven. In vitro toxicology evaluation of pharmaceuticals using Raman micro-spectroscopy. Journal of cellular biochemistry. vol 99. issue 1. 2006-11-06. PMID:16598770. |
dna fragmentation was confirmed by western blot analysis of apoptosis regulator protein p53 and cell metabolic activity determined by mtt assay. |
2006-11-06 |
2023-08-12 |
human |