| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Fuzhong Qin, Megan Simeone, Ravish Pate. Inhibition of NADPH oxidase reduces myocardial oxidative stress and apoptosis and improves cardiac function in heart failure after myocardial infarction. Free radical biology & medicine. vol 43. issue 2. 2007-10-31. PMID:17603936. |
myocardial nadph oxidase activity was measured by superoxide dismutase-inhibitable cytochrome c reduction assay, nadph oxidase subunit p47phox expression by western blot and immunofluorescence analysis, myocardial oxidative stress evaluated by 8-hydroxydeoxyguanosine (8-ohdg) and 4-hydroxy-2-nonenal (4-hne) using immunohistochemistry, and myocyte apoptosis by tunel assay. |
2007-10-31 |
2023-08-12 |
Not clear |
| David Y Zhang, Yong Li, Syed M Abbas Rizvi, Changfa Qu, John Kearsley, Barry J Alle. Cytotoxicity of breast cancer cells overexpressing HER2/neu by 213Bi-Herceptin radioimmunoconjugate. Cancer letters. vol 218. issue 2. 2007-10-30. PMID:15670895. |
we used western blot and flow cytometry analysis to examine the expression of her2 in a panel of established human metastatic breast cancer cell lines (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2h-tetrazolium, inner salt) mts assay to evaluate the cytotoxicity and the tunel assay to analyze cellular apoptosis. |
2007-10-30 |
2023-08-12 |
human |
| Margherita Sisto, Sabrina Lisi, Dario Lofrumento, Massimo D'Amore, Pasquale Scagliusi, Vincenzo Mitol. Autoantibodies from Sjögren's syndrome trigger apoptosis in salivary gland cell line. Annals of the New York Academy of Sciences. vol 1108. 2007-10-30. PMID:17894006. |
apoptosis induced by autoantibodies was revealed by facs analysis, and the active caspase-3 and the cleaved caspase-3 substrate poly (adp-ribose) polymerase (parp) was demonstrated by colorimetric assay and western blot. |
2007-10-30 |
2023-08-12 |
human |
| Michaela Artwohl, Kathrin Muth, Karin Kosulin, Rainer de Martin, Thomas Hölzenbein, Georg Rainer, Angelika Freudenthaler, Nicole Huttary, Leopold Schmetterer, Werner K Waldhäusl, Sabina M Baumgartner-Parze. R-(+)-alpha-lipoic acid inhibits endothelial cell apoptosis and proliferation: involvement of Akt and retinoblastoma protein/E2F-1. American journal of physiology. Endocrinology and metabolism. vol 293. issue 3. 2007-10-24. PMID:17566113. |
apoptosis, proliferation, cell cycle distribution, and protein expression were determined by dna fragmentation assays, [(3)h]thymidine incorporation, facs, and western blot analyses, respectively. |
2007-10-24 |
2023-08-12 |
human |
| Xian-Qi Feng, Yong You, Juan Xiao, Ping Zo. [Thapsigargin-induced apoptosis of K562 cells and its mechanism]. Zhongguo shi yan xue ye xue za zhi. vol 14. issue 1. 2007-10-19. PMID:16584585. |
after the treatment of leukemia cell line k562 by thapsigargin, morphological change of apoptotic cells was investigated by ao/eb fluorescent staining under fluorescent microscope; apoptosis rate was determined with annexin v-fitc/pi double staining by flow cytometry; intracellular calcium concentrations ([ca(2+)]i) were measured by fluorescence spectrophotometer with calcium sensitive fluorescence indicator fura-2/am; mitochondrial transmembrance potentials (delta psi m) was detected on flow cytometry through staining of rhodamine (rh123); the changes of caspase-3, -7, -9, -12, cytochrome c, grp78 proteins were detected by western blot. |
2007-10-19 |
2023-08-12 |
Not clear |
| Weike Mao, Shuji Fukuoka, Chikao Iwai, Jiahao Liu, Virendra K Sharma, Shey-Shing Sheu, Michael Fu, Chang-seng Lian. Cardiomyocyte apoptosis in autoimmune cardiomyopathy: mediated via endoplasmic reticulum stress and exaggerated by norepinephrine. American journal of physiology. Heart and circulatory physiology. vol 293. issue 3. 2007-10-19. PMID:17545481. |
myocyte apoptosis was determined by terminal deoxytransferase-mediated dutp nick-end labeling and caspase-3 activity, whereas camkii, mapk family (jnk, p38, erk), and er stress signals (atf6, grp78, chop, caspase-12) were measured by western blot, immunohistochemistry, and kinase activity assay. |
2007-10-19 |
2023-08-12 |
Not clear |
| Zheyu Wang, Krista Butt, Lili Wang, Hu Li. The effect of seal oil on paclitaxel induced cytotoxicity and apoptosis in breast carcinoma MCF-7 and MDA-MB-231 cell lines. Nutrition and cancer. vol 58. issue 2. 2007-10-09. PMID:17640170. |
western blot analysis showed that the expression of b cell lymphoma-2 (bcl-2) protein, an apoptosis inhibitory protein, in both cell lines was decreased more significant by paclitaxel in combination with seal oil than by paclitaxel alone. |
2007-10-09 |
2023-08-12 |
Not clear |
| Xingrong Lu, Jilin Y. SUMO-1 enhancing the p53-induced HepG2 cell apoptosis. Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban. vol 25. issue 3. 2007-10-02. PMID:16201274. |
western blot was employed to detect the protein expression of the transfected recombinant plasmids and the rate of apoptosis was measured by flow cytometry. |
2007-10-02 |
2023-08-12 |
Not clear |
| Manchao Zhang, Xueliang Fang, Hongpeng Liu, Ribo Guo, Xiaojin Wu, Bihua Li, Feng Zhu, Yan Ling, Brian N Griffith, Shaomeng Wang, Dajun Yan. Bioinformatics-based discovery and characterization of an AKT-selective inhibitor 9-chloro-2-methylellipticinium acetate (CMEP) in breast cancer cells. Cancer letters. vol 252. issue 2. 2007-10-01. PMID:17293030. |
the possible effects of potent compound on pi3k/akt, pdk1, and mapk, its antiproliferative and apoptosis-inducing effects on breast cancer cells which have high-levels of akt activation were assessed by western blot analysis, cell viability assay, and apoptosis assay. |
2007-10-01 |
2023-08-12 |
human |
| Sheng-Huei Yang, Chien-Hsun Tsai, Mei-Chin Lu, Yung-Ning Yang, Ching-Ming Chien, Sheng-Fung Lin, Shinne-Ren Li. Effects of cardiotoxin III on expression of genes and proteins related to G2/M arrest and apoptosis in K562 cells. Molecular and cellular biochemistry. vol 300. issue 1-2. 2007-09-25. PMID:17149543. |
in the present study, the expression of mrnas and proteins related to cell cycle and apoptosis in human leukemia k562 cells induced by ctx iii was investigated by semi-quantitative reverse transcription-polymerase chain reaction (rt-pcr) and western blot analysis. |
2007-09-25 |
2023-08-12 |
human |
| Lisa M Dauffenbach, Shah M Khan, John Ye. Corpus luteum regression in the rat--in vivo and in vitro studies of apoptotic mechanisms. Journal of medicine. vol 34. issue 1-6. 2007-09-21. PMID:17682315. |
apoptosis was measured by annexin v and propidium iodide staining, loss of fluorescence of 10-n-nonyl acridine orange (nao), a cardiolipin specific fluorescent dye, and cleavage of pro-caspase-9 by western blot analysis. |
2007-09-21 |
2023-08-12 |
rat |
| Kyle C Cuneo, Allie Fu, Katherine Osusky, Jessica Huamani, Dennis E Hallahan, Ling Gen. Histone deacetylase inhibitor NVP-LAQ824 sensitizes human nonsmall cell lung cancer to the cytotoxic effects of ionizing radiation. Anti-cancer drugs. vol 18. issue 7. 2007-09-20. PMID:17581301. |
western blot analysis of caspase cleavage, microscopic analysis of nuclei and annexin-fluorescein isothiocyanate/propidium iodide flow cytometry assays showed that laq824 enhanced radiation-induced apoptosis and attenuated mitosis (p<0.001). |
2007-09-20 |
2023-08-12 |
mouse |
| Robert L Copeland, Jharna R Das, Yasmine M Kanaan, Robert E Taylor, Yousef Tizab. Antiapoptotic effects of nicotine in its protection against salsolinol-induced cytotoxicity. Neurotoxicity research. vol 12. issue 1. 2007-09-18. PMID:17513200. |
sh-sy5y cells were exposed to 0.4 or 0.7 mm salsolinol with and without pretreatment in combination of 0.1 mm nicotine and 0.1 mm mecamylamine and were exposed for 24 and 48 h. various parameters including cell cycle perturbations (reflected in propidium iodide dna staining); cell cycle regulator retinoblastoma protein (reflected in the western blot), apoptosis (reflected in annexin v/propidium iodide staining followed by flow cytometry) were analyzed. |
2007-09-18 |
2023-08-12 |
human |
| Kiyotaka Nakagawa, Akira Shibata, Shinji Yamashita, Tsuyoshi Tsuzuki, Jun Kariya, Shinichi Oikawa, Teruo Miyazaw. In vivo angiogenesis is suppressed by unsaturated vitamin E, tocotrienol. The Journal of nutrition. vol 137. issue 8. 2007-09-13. PMID:17634267. |
western blot analysis revealed that delta-t3 suppressed the phosphorylation of phosophoinositide-dependent protein kinase (pdk) and akt, and increased the phosphorylation of apoptosis signal-regulating kinase and p38 in fibroblast growth factor-treated huvec, indicating that the antiangiogenic effects of t3 are associated with changes in growth factor-dependent phosphatidylinositol-3 kinase /pdk/akt signaling as well as induction of apoptosis in endothelial cells. |
2007-09-13 |
2023-08-12 |
mouse |
| Young-Joo Bae, Soon-Ja Kang, Kwang Sook Par. Drosophila melanogaster Parkin ubiquitinates peanut and septin1 as an E3 ubiquitin-protein ligase. Insect biochemistry and molecular biology. vol 37. issue 5. 2007-08-23. PMID:17456438. |
western blot analysis and dna fragmentation indicated that the rate and efficiency of p53-dependent apoptosis were significantly higher in the presence of dopamine than without the septin proteins. |
2007-08-23 |
2023-08-12 |
human |
| Marie P Piechocki, George H Yoo, Susan K Dibbley, Fulvio Lonard. Breast cancer expressing the activated HER2/neu is sensitive to gefitinib in vitro and in vivo and acquires resistance through a novel point mutation in the HER2/neu. Cancer research. vol 67. issue 14. 2007-08-21. PMID:17638894. |
we characterized the effects of gefitinib on egfr, her2, and erbb-3 phosphorylation by western blot and determined the effects on downstream signaling through growth, survival, and stress pathways and the effect on proliferation, cell cycle, and apoptosis. |
2007-08-21 |
2023-08-12 |
Not clear |
| R T Kilani, M Mackova, S T Davidge, B Winkler-Lowen, N Demianczuk, L J Guilber. Endogenous tumor necrosis factor alpha mediates enhanced apoptosis of cultured villous trophoblasts from intrauterine growth-restricted placentae. Reproduction (Cambridge, England). vol 133. issue 1. 2007-08-15. PMID:17244751. |
a higher level of tnfalpha receptor p55 (which mediates apoptosis) was found in iugr ct by western blot analysis at po2 of <10, 38, and 140 mmhg. |
2007-08-15 |
2023-08-12 |
Not clear |
| Angelika Hammerer-Lercher, Christa Haeusler, Martina Prelog, Johannes Bonatti, Daniel Hoefer, Elfriede Ruttmann, Guenther Laufer, Ernst R Werner, Stephan Dirnhofer, Bernd Puschendorf, Johannes Mai. Thermal preconditioning protects the human internal mammary artery from hypoxia/re-oxygenation-induced damage. Clinical and experimental pharmacology & physiology. vol 33. issue 7. 2007-08-09. PMID:16789924. |
western blot, real-time quantative polymerase chain reaction analysis and apoptosis detection by the terminal deoxyribonucleotidyl transferase-mediated dutp-digoxigenin nick end-labelling method were performed. |
2007-08-09 |
2023-08-12 |
human |
| Seyed Mohamed Ali, Malini Olivo, Gan Yik Yuen, Soo Khee Che. Apoptosis induced by photosensitizers (Perylquinone derivatives) in human carcinoma cells: a possible relevance to photodynamic therapy. Asian journal of surgery. vol 25. issue 1. 2007-08-08. PMID:17585441. |
western blot analysis of poly (adp-ribose) polymerase, a caspases substrate, showed the classical cleavage pattern (116 to 85kda) associated with apoptosis in ha and hb-treated cell lysates. |
2007-08-08 |
2023-08-12 |
human |
| Elizabeth Perron, M Behzad Zafar, Amanda Pister, Zhen-Guo Wang, Jun Tian, Prem Set. Evaluating apoptosis in tumor cells infected with adenovirus expressing p53 tumor suppressor gene. Methods in molecular medicine. vol 130. 2007-08-01. PMID:17401169. |
we include cytotoxicity assays and assays of apoptosis, including dna-nucleosomal dna fragmentation (dna laddering), tunel, dapi staining, analysis of the sub-g1 (subdiploid) population, and degradation of poly(adp-ribose) polymerase (as assayed by western blot). |
2007-08-01 |
2023-08-12 |
Not clear |