| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Tsung-Hsing Lin, Chin-Ho Kuo, Yi-Sheng Zhang, Pin-Tzu Chen, Shu-Hsin Chen, Yi-Zhen Li, Ying-Ray Le. Piperlongumine Induces Cellular Apoptosis and Autophagy via the ROS/Akt Signaling Pathway in Human Follicular Thyroid Cancer Cells. International journal of molecular sciences. vol 24. issue 9. 2023-05-14. PMID:37175755. |
therefore, in the present study, we evaluated the effect of piperlongumine on cell proliferation, cell cycle, apoptosis, and autophagy in ftc cells with flowcytometry and western blot. |
2023-05-14 |
2023-08-14 |
human |
| Daniela Giovannini, Cinzia De Angelis, Maria Denise Astorino, Emiliano Fratini, Evaristo Cisbani, Giulia Bazzano, Alessandro Ampollini, Massimo Piccinini, Enrico Nichelatti, Emiliano Trinca, Paolo Nenzi, Mariateresa Mancuso, Luigi Picardi, Carmela Marino, Concetta Ronsivalle, Simonetta Pazzagli. In Vivo Radiobiological Investigations with the TOP-IMPLART Proton Beam on a Medulloblastoma Mouse Model. International journal of molecular sciences. vol 24. issue 9. 2023-05-14. PMID:37175984. |
apoptosis was visualized by immunohistochemistry for the apoptotic marker caspase-3-activated, and quantified by western blot. |
2023-05-14 |
2023-08-14 |
mouse |
| Heng Yang, Lin Fu, Licai Li, Dezhi Zhang, Qianyong Li, Peng Zho. miR-665 overexpression inhibits the apoptosis of luteal cells in small ruminants suppressing HPGDS. Theriogenology. vol 206. 2023-05-13. PMID:37178673. |
following mir-665 overexpression, the apoptosis rate of the luteal cells was determined using flow cytometry; b-cell lymphoma-2 (bcl-2) and caspase-3 mrna and protein were measured using qrt-pcr and western blot (wb) analysis. |
2023-05-13 |
2023-08-14 |
Not clear |
| Lei Fu, Lina Zhao, Chunyan Liao, Pengzhen Wang, Ying Gu, Sha Li, Liying Shi, Qiaorong Wang, Jin Xie, Lixin Zhang, Xiaoming Liu, Bei Zhan. Knockdown of KAT5/KIF11 induces autophagy and promotes apoptosis in anaplastic thyroid cancer cells. Experimental and therapeutic medicine. vol 25. issue 6. 2023-05-09. PMID:37153895. |
flow cytometry and western blot analyses were applied for the assessment of cell apoptosis. |
2023-05-09 |
2023-08-14 |
Not clear |
| Chunjie Sui, Dan Zho. ADAM metallopeptidase domain 10 knockdown enables podocytes to resist high glucose stimulation by inhibiting pyroptosis via MAPK pathway. Experimental and therapeutic medicine. vol 25. issue 6. 2023-05-09. PMID:37153901. |
moreover, the effects of adam10 knockdown on podocyte inflammation and apoptosis were determined by elisa, western blot and tunel assay after confirming the efficacy of cell transfection. |
2023-05-09 |
2023-08-14 |
Not clear |
| Xiongfei Zhang, Jianping Chen, Beili Xi, Yutong Liu, Shaojun Wang, Ling Gu, Huanhuan Zhao, Li Tao, Yang Hua, Yan Wang, Meijuan Che. Agrimoniin is a dual inhibitor of AKT and ERK pathways that inhibit pancreatic cancer cell proliferation. Phytotherapy research : PTR. 2023-05-08. PMID:37156642. |
in this study, we used 5-ethynyl-2'-deoxyuridine, cell counting kit-8, flow cytometry, and western blot experiments to confirm that agrimoniin can significantly inhibit the proliferation of pancreatic cancer cell panc-1 by inducing apoptosis and cell cycle arrest. |
2023-05-08 |
2023-08-14 |
Not clear |
| Boxin Li, Hui Yang, Haipeng Wu, Huanhuan Wang, Zhongming Ye, Yao Wu, Yuting Chen, Huanwen Tan. Hydroquinone-induced endoplasmic reticulum stress affects TK6 cell autophagy and apoptosis via ATF6-mTOR. Environmental toxicology. 2023-05-06. PMID:37148176. |
we treated tk6 cells with hq to establish a cytotoxicity model and found that hq induced cellular ers, autophagy and apoptosis by western blot, flow cytometry and transmission electron microscopy. |
2023-05-06 |
2023-08-14 |
human |
| Youping Wang, Chi Pan, Xia Zhang, Aiqi Zhao, Ying Don. Mutant NPM1 maintains RASGRP3 protein stability via interaction with MID1 to promote acute myeloid leukemia cell proliferation and autophagy. Journal of leukocyte biology. vol 113. issue 5. 2023-05-05. PMID:36826998. |
further, cck8, edu staining, immunofluorescence, and western blot were performed to explore the effect of rasgrp3 on cell proliferation and apoptosis in npm1-mutated acute myeloid leukemia. |
2023-05-05 |
2023-12-17 |
Not clear |
| Lixia Zhao, Ju Cheng, Di Liu, Hongxia Gong, Decheng Bai, Wei Su. Potentilla anserina polysaccharide alleviates cadmium-induced oxidative stress and apoptosis of H9c2 cells by regulating the MG53-mediated RISK pathway. Chinese journal of natural medicines. vol 21. issue 4. 2023-04-29. PMID:37120246. |
western blot was performed to detect the expression of proteins related to mg53, the risk pathway, and apoptosis. |
2023-04-29 |
2023-08-14 |
cat |
| Małgorzata Opydo, Anna Mlyczyńska, Ewa Mlyczyńska, Agnieszka Rak, Elzbieta Kolaczkowsk. Synergistic Action of MCL-1 Inhibitor with BCL-2/BCL-XL or MAPK Pathway Inhibitors Enhances Acute Myeloid Leukemia Cell Apoptosis and Differentiation. International journal of molecular sciences. vol 24. issue 8. 2023-04-28. PMID:37108344. |
to assess aml cells' apoptosis and differentiation, in vitro studies were performed using prestoblue assay, coulter electrical impedance method, flow cytometry, light microscopy and western blot techniques. |
2023-04-28 |
2023-08-14 |
Not clear |
| Antong Wu, Janak Lal Pathak, Xingyang Li, Wei Cao, Wenchao Zhong, Mingjing Zhu, Qiuyu Wu, Wanyi Chen, Qiao Han, Siqing Jiang, Yuzhuo Hei, Ziyi Zhang, Gang Wu, Qingbin Zhan. Human Salivary Histatin-1 Attenuates Osteoarthritis through Promoting M1/M2 Macrophage Transition. Pharmaceutics. vol 15. issue 4. 2023-04-28. PMID:37111757. |
furthermore, cell migration assay, alcian blue, safranin o staining, rt-qpcr, western blot, and fcm showed that hst1 not only attenuates m1-macrophage-cm-induced apoptosis and matrix metalloproteinase expression in chondrogenic cells, but it also restores their metabolic activity, migration, and chondrogenic differentiation. |
2023-04-28 |
2023-08-14 |
human |
| Mingyue Sun, Honglin Li, Baorong Fen. [miR-30e-3p in natural killer cell-derived exosomes inhibits the proliferation and invasion of human esophageal squamous carcinoma cells]. Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology. vol 39. issue 4. 2023-04-23. PMID:37087546. |
western blot analysis was used to detect the expression levels of exosome markers apoptosis related gene 2- interacting protein x(alix), tumor susceptibility gene 101(tsg101), cd81 and calnexin. |
2023-04-23 |
2023-08-14 |
human |
| Azadeh Emami, Hossein Ghafouri, Reyhaneh Sarir. Polyphyllin D-Loaded Solid Lipid Nanoparticles for Breast Cancer: Synthesis, Characterization, In Vitro, and in Vivo Studies. International journal of pharmaceutics. 2023-04-23. PMID:37088118. |
apoptosis, as the mechanism of cell death, was confirmed by flow cytometry following annexin v/propidium iodide staining and western blot analysis. |
2023-04-23 |
2023-08-14 |
mouse |
| Huiqiong Deng, Wenying Chen, Boyang Zhang, Yiwen Zhang, Lingyun Han, Qipeng Zhang, Song Yao, Hongwei Wang, Xiao Li She. Excessive ER-phagy contributes to ochratoxin A-induced apoptosis. Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association. 2023-04-20. PMID:37080527. |
in the present study, the cell viability and protein expressions of human proximal tubule epithelial (hk-2) cells in response to ota and/or chloroquine/rapamycin/sodium phenylbutyrate/tunicamycin were determined via cell viability assay, apoptosis analysis, and western blot analysis. |
2023-04-20 |
2023-08-14 |
human |
| Chao Zhang, Shuai W. RUVBL1-modulated chromatin remodeling alters the transcriptional activity of oncogenic CTNNB1 in uveal melanoma. Cell death discovery. vol 9. issue 1. 2023-04-19. PMID:37076452. |
cck-8 assay, flow cytometry, scratch assay, transwell assay and western blot analysis were used for detection on the resultant uvm cell proliferation, apoptosis, migration, invasion and cell cycle distribution. |
2023-04-19 |
2023-08-14 |
Not clear |
| Zhongqiu Tan, Lifeng Zhao, Shiqing Huang, Qiulan Jiang, Yantao Wei, Junyun Long Wu, Zhiwen Zhang, Yepeng L. Small peptide LINC00511-133aa encoded by LINC00511 regulates breast cancer cell invasion and stemness through the Wnt/β-catenin pathway. Molecular and cellular probes. 2023-04-17. PMID:37068562. |
cell proliferation, invasion, and apoptosis were evaluated using cck8, apoptotic, wound healing, and transwell invasion assays, while the characteristic changes of tumor stem cells were detected through sphere-forming assay and western blot analyses of the stemness markers oct4, nanog, and sox2. |
2023-04-17 |
2023-08-14 |
Not clear |
| Jing-Yu Qu, Hua-Tao Xie, Yu-Ting Xiao, Ying-Ying Zhang, Zhi-Xin Hu, Jia-Song Wang, Ming-Chang Zhang, Han X. The inhibition of p38 MAPK blocked inflammation to restore the functions of rat meibomian gland epithelial cells. Experimental eye research. 2023-04-14. PMID:37059216. |
cell proliferation, keratinization, lipid accumulation, and matrix metalloproteinases 9 (mmp9) expression were evaluated by mtt assay, polymerase chain reaction (pcr), immunofluorescence staining, apoptosis assay, lipid staining, and western blot analyses. |
2023-04-14 |
2023-08-14 |
mouse |
| Dong Han, Ming Huang, Zhen Chang, Wei Su. KLF15 Transcriptionally Activates ATG14 to Promote Autophagy and Attenuate Damage of ox-LDL-Induced HAECs. Molecular biotechnology. 2023-04-12. PMID:37043109. |
klf15 was overexpressed in cells by lipofection transfection, and then cck8, flow cytometry, western blot, elisa, and related assay kits were used to detect cell viability, apoptosis, inflammatory response as well as oxidative stress, respectively. |
2023-04-12 |
2023-08-14 |
human |
| Akira Sakamoto, Hiroyuki Inoue, Shohei Miyamoto, Shun Ito, Yasushi Soda, Kenzaburo Tan. Coxsackievirus A11 is an immunostimulatory oncolytic virus that induces complete tumor regression in a human non-small cell lung cancer. Scientific reports. vol 13. issue 1. 2023-04-12. PMID:37046036. |
in vitro inhibition analysis using a pan-caspase inhibitor and western blot detection of cleaved poly (adp-ribose) polymerase (parp) indicated that apoptosis partly contributed to cva11-driven cytotoxicity. |
2023-04-12 |
2023-08-14 |
mouse |
| E Qin, Shuojia Gu, Yimin Guo, Liyan Wang, Guimei P. MiRNA-30a-5p/VCAN Arrests Tumor Metastasis via Modulating the Adhesion of Lung Adenocarcinoma Cells. Applied biochemistry and biotechnology. 2023-04-09. PMID:37032373. |
several in vitro experiments were applied for cell function detection: dual-luciferase assay for validating the targeting relationship between mirna-30a-5p and its target gene; quantitative real-time polymerase chain reaction for testing the expression of mirna-30a-5p and its target gene in luad cells; mtt, transwell, cell adhesion, flow cytometry and immunofluorescence assays for examining the capabilities of luad cells to proliferate, migrate, invade, adhere, apoptosis and epithelial-mesenchymal transition (emt) effect; western blot for determining the expression of adhesion-related proteins and emt-related proteins. |
2023-04-09 |
2023-08-14 |
Not clear |