| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Taibang Chen, Xiaoqing He, Jing Wang, Di Du, Yongqing X. NT-3 Combined with TGF-β Signaling Pathway Enhance the Repair of Spinal Cord Injury by Inhibiting Glial Scar Formation and Promoting Axonal Regeneration. Molecular biotechnology. 2023-06-15. PMID:37318740. |
immunofluorescence, tunel and western blot analysis showed that compared with model group, the neun expression increased, and the apoptosis and col iv, ln, cspg, tenascin-c, sema 3 a, ephb2 and smad2/3 protein expression decreased significantly in the nt-3 and nt-3 + ly364947 groups; the condition was reversed in the nt-3 + tgf-β1 group compared with the nt-3 group. |
2023-06-15 |
2023-08-14 |
mouse |
| Zhaoqing Gong, Xinlei Shi, Weizhen Xu, Yuan Fang, Meijia Fang, Minhua Yao, Yu Jiang, Hongshu Sui, Mingjiu Lu. LncRNA PWRN2 promotes polycystic ovary syndrome progression via epigenetically reducing ATRX by recruiting LSD1. Reproductive biology. vol 23. issue 3. 2023-06-15. PMID:37320994. |
the protein levels of apoptosis markers and alpha thalassemia retardation syndrome x-linked (atrx) were determined by western blot. |
2023-06-15 |
2023-08-14 |
rat |
| Hajime Ishinaga, Yoshinaga Okugawa, Bo Hou, Feng He, Chengzeng Yin, Mariko Murata, Yuji Toiyama, Kazuhiko Takeuch. The role of miR-21 as a predictive biomarker and a potential target to improve the effects of chemoradiotherapy against head and neck squamous cell carcinoma. Journal of radiation research. 2023-06-14. PMID:37312615. |
western blot analysis suggested the programmed cell death 4 protein as a potential target of mir-21 in relation to apoptosis. |
2023-06-14 |
2023-08-14 |
human |
| Jialiang Lin, Xuanqi Zheng, Zhencheng Xiong, Qian Xiang, Yongzhao Zhao, Shuai Jiang, Zhuoran Sun, Dongwei Fan, Chuiguo Sun, Weishi L. DJ-1-mediated p62 degradation delays intervertebral disc degeneration by inhibiting apoptosis of nucleus pulposus cells. Apoptosis : an international journal on programmed cell death. 2023-06-10. PMID:37300741. |
after overexpression of dj-1 in npcs by lentiviral transfection, dcfh-da and mitosox fluorescent probes were used to evaluate the levels of reactive oxygen species (ros); while western blot, tunel staining, and caspase-3 activity were used to assess apoptosis. |
2023-06-10 |
2023-08-14 |
rat |
| Liangzi Cao, Hao Li, Xiaofang Liu, Yubang Wang, Bowen Zheng, Chengzhong Xing, Naijin Zhang, Jingwei Li. Expression and regulatory network of E3 ubiquitin ligase NEDD4 family in cancers. BMC cancer. vol 23. issue 1. 2023-06-08. PMID:37291499. |
nedd4 family members interacted proteins were enriched in various pathways including p53, akt, apoptosis and autophagy, which were confirmed by further western blot and flow cytometric analysis in a549 and h1299 lung cancer cells. |
2023-06-08 |
2023-08-14 |
Not clear |
| Zong-Xing Zhang, Lu Jiang, Dao-Zhong Liu, Bo-Nan Tao, Zi-Ming Hou, Meng-Jie Tian, Jia Feng, Lin Yua. [Alcohol extract of root and root bark of Toddalia asiatica alleviates CIA in rats through anti-inflammatory and proapoptotic effects]. Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica. vol 48. issue 8. 2023-06-07. PMID:37282908. |
in addition, rt-qpcr and western blot results showed that taae up-regulated the level of bax, down-regulated the level of bcl-2, and activated caspase-3 to promote apoptosis in synoviocytes. |
2023-06-07 |
2023-08-14 |
rat |
| Haoyu Zhang, Kexin Sun, Meichen Gao, Shiwen X. Zinc Inhibits Lead-Induced Oxidative Stress and Apoptosis of ST Cells Through ROS/PTEN/PI3K/AKT Axis. Biological trace element research. 2023-06-03. PMID:37269454. |
based on this information, we treated st cells with pb and zn and detected related indices such as apoptosis, oxidative stress, and the pten/pi3k/akt pathway using flow cytometry, dcfh-da staining, rt-pcr, and western blot. |
2023-06-03 |
2023-08-14 |
human |
| Ling Zuo, Yang Hai, Ruoyu Zhang, Biao Zuo, Jiayu Tian, Pan Li, Xiumei Ke, Meng Wang, Li Ren, Xuezhi Li, Xuekuan Huang, Jianwei Wan. Therapeutic potential of icariin in rats with letrozole and high-fat diet-induced polycystic ovary syndrome. European journal of pharmacology. 2023-06-03. PMID:37269973. |
combined with the ovarian transcriptome, we verified the key markers of apoptosis and the janus kinase/signal transducer and activator of transcription (jak/stat) signaling pathway by rt-qpcr for mrna level, western blot, and immunohistochemistry for protein expression. |
2023-06-03 |
2023-08-14 |
rat |
| Xiaozhou Wang, Hongyan Li, Jie Wang, Huiling Xu, Kun Xue, Xiaoting Liu, Zhizhong Zhang, Jianzhu Liu, Yongxia Li. Staphylococcus aureus extracellular vesicles induce apoptosis and restrain mitophagy-mediated degradation of damaged mitochondria. Microbiological research. vol 273. 2023-06-02. PMID:37267814. |
meanwhile, western blot and confocal microscopy detected mitochondrial damage, apoptosis, and parkin-mediated mitophagy. |
2023-06-02 |
2023-08-14 |
cattle |
| Yanyun Zhao, Sihan Huang, Renwei Xie, Jun Li. Extracellular ATP accelerates cell death and decreases tight junction protein ZO-1 in hypoxic cochlear strial marginal cells in neonatal rats. Cellular signalling. 2023-05-28. PMID:37245680. |
flow cytometry and western blot analyses revealed an increase in apoptosis levels and suppression of autophagy, indicating that eatp causes additional cell death by increasing the apoptosis of hypoxic mcs. |
2023-05-28 |
2023-08-14 |
rat |
| Qi Wang, Jia-Fei Xie, Ting-Ting Yao, Xu-Xu Wang, Qian-Wen Guo, Lu-Shan Wang, Yue Yu, Li-Chun X. MicroRNA‑30a‑5p regulates cypermethrin-induced apoptosis of Sertoli cells by targeting KLF9 in vitro. Reproductive toxicology (Elmsford, N.Y.). 2023-05-28. PMID:37245696. |
the apoptosis of tm4 cells, the expression level of mir-30a-5p, the protein expressions and the interaction between mir-30a-5p and klf9 were detected by flow cytometry, quantitative real-time pcr, western blot and luciferase reporter assays. |
2023-05-28 |
2023-08-14 |
mouse |
| Vicente Andreu-Fernández, Mariona Serra-Delgado, Laura Almeida-Toledano, Àgueda García-Meseguer, Melina Vieiros, Anna Ramos-Triguero, Concha Muñoz-Lozano, Elisabet Navarro-Tapia, Leopoldo Martínez, Óscar García-Algar, María D Gómez-Roi. Effect of Postnatal Epigallocatechin-Gallate Treatment on Cardiac Function in Mice Prenatally Exposed to Alcohol. Antioxidants (Basel, Switzerland). vol 12. issue 5. 2023-05-27. PMID:37237934. |
heart biomarkers of apoptosis, oxidative stress, and cardiac damage were analyzed by western blot. |
2023-05-27 |
2023-08-14 |
mouse |
| Shujuan Liu, Qiqi Bu, Jiashun Tong, Zhanhang Wang, Jiuzeng Cui, Heran Cao, Haidong Ma, Binyun Cao, Xiaopeng An, Yuxuan Son. miR-486 Responds to Apoptosis and Autophagy by Repressing SRSF3 Expression in Ovarian Granulosa Cells of Dairy Goats. International journal of molecular sciences. vol 24. issue 10. 2023-05-27. PMID:37240097. |
here, we identified and characterized mir-486 interaction with ser/arg-rich splicing factor 3 (srsf3) using luciferase reporter analysis, detecting its role in gc survival, apoptosis and autophagy regulation through qrt-pcr, western blot, cck-8, edu, flow cytometry, mitochondrial membrane potential and monodansylcadaverine, etc. |
2023-05-27 |
2023-08-14 |
Not clear |
| Abdelhabib Semlali, Ikram Ajala, Sarra Beji, Mohammed Mousa Al-Zharani, Mahmoud Rouabhi. Synergistic Effect of Anethole and Platinum Drug Cisplatin against Oral Cancer Cell Growth and Migration by Inhibiting MAPKase, Beta-Catenin, and NF-κB Pathways. Pharmaceuticals (Basel, Switzerland). vol 16. issue 5. 2023-05-27. PMID:37242484. |
apoptosis, caspase activity, oxidative stress, mitosox, and mitochondrial membrane potential (ΔΨm) levels were evaluated by flow cytometry, and the inhibition of signaling pathways was investigated by western blot. |
2023-05-27 |
2023-08-14 |
Not clear |
| Yan Li, Ying Zhang, Lei Gua. MiR-708 regulates the biological behavior of childhood leukemia cells by binding to the 3'utr end of the target gene and reducing the level of the target gene. Cellular and molecular biology (Noisy-le-Grand, France). vol 69. issue 2. 2023-05-25. PMID:37224029. |
mtt assay was used to detect the cell proliferation inhibition rate, flow cytometry was used to detect the apoptosis rate and cell cycle change, the scratch test was used to detect the cell migration capacity, and western blot assay was used to detect the expression of cntfr, apoptosis and jak/stat pathway related proteins. |
2023-05-25 |
2023-08-14 |
human |
| Jiaxuan Wu, Shengya Fang, Pengfei Feng, Chunjiang Cai, Leying Zhang, Ling Yan. Changes in expression levels of Nod-like receptors in the spleen of ewes. Animal reproduction. vol 20. issue 1. 2023-05-25. PMID:37228386. |
expression of nlr family, including nod1, nod2, class ii transactivator (ciita), nlr family apoptosis inhibitory protein (naip), nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domain containing 1 (nlrp1), nlrp3 and nlrp7, was analyzed using quantitative real-time pcr, western blot and immunohistochemistry analysis. |
2023-05-25 |
2023-08-14 |
Not clear |
| Qiran Sun, Jiaqi Li, Hao Dong, Jiao Zhan, Xiaoming Xiong, Jiashan Ding, Yuan Li, Linsheng He, Jing Wan. UQCRFS1 serves as a prognostic biomarker and promotes the progression of ovarian cancer. Scientific reports. vol 13. issue 1. 2023-05-23. PMID:37221238. |
cell proliferation was detected by cck8 assay, cell cycle and apoptosis were determined by flow cytometry, reactive oxygen species (ros) production was detected by dcfh-da, dna damage gene mrna expression was analyzed by rt-pcr, and akt/mtor pathway protein expression were also examined by western blot after sirna transfection. |
2023-05-23 |
2023-08-14 |
Not clear |
| Jianping Xiao, Deguang Wan. LncRNA GAS5 as an inflammatory regulator acting through the MAPK pathway in human lupus. Current pharmaceutical design. 2023-05-18. PMID:37198993. |
objective find the specific mechanism of action of lncrna gas5 in sle method collecting samples of the sle patients, cell culture and treatment, plasmid construction, and transfection, quantitative real-time pcr analysis, enzyme-linked immunosorbent assay (elisa), cell viability analysis, cell apoptosis analysis, western blot. |
2023-05-18 |
2023-08-14 |
human |
| Jialin Zheng, Shijian Zhao, Qishi Yang, Yantao Wei, Jianmei Li, Tao Gu. Sympathetic Activation Promotes Cardiomyocyte Apoptosis in a Rabbit Susceptibility Model of Hyperthyroidism-Induced Atrial Fibrillation via the p38 MAPK Signaling Pathway. Critical reviews in eukaryotic gene expression. vol 33. issue 5. 2023-05-18. PMID:37199311. |
primary rabbit cardiomyocytes were cultured and identified by immunofluorescence staining, and terminal deoxynucleotidyl transferase dutp nick end labeling staining was used to measure cardiomyocyte apoptosis; western blot was used to detect the expression of apoptosis-related proteins, including bax, bcl-2, and cleaved caspase-3, as well as to measure the phosphorylation states of p38 mitogen-activated protein kinase (mapk) pathway proteins. |
2023-05-18 |
2023-08-14 |
rabbit |
| Yanling Li, Jing Gao, Chuntian Liu, Ning Bu, Shuqin Zhan, Haiqin Wu, Ru Zhang, Hong Sun, Hong Fa. USP22 knockdown protects against cerebral ischemia/reperfusion injury via destabilizing PTEN protein and activating the mTOR/TFEB pathway. Naunyn-Schmiedeberg's archives of pharmacology. 2023-05-16. PMID:37191727. |
the effects of usp22 on proliferation, apoptosis, oxidative stress, and autophagy were explored by cck-8, flow cytometry, elisa, and western blot assays. |
2023-05-16 |
2023-08-14 |
mouse |