| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Ren-Ping Zheng, Tao Bai, Xiao-Guang Zhou, Chang-Gen Xu, Wei Wang, Ming-Wei Xu, Jie Zhan. Lefty A protein inhibits TGF-β1-mediated apoptosis in human renal tubular epithelial cells. Molecular medicine reports. vol 8. issue 2. 2014-02-13. PMID:23807264. |
following exposure of the hk-2 cells to recombinant human tgf-β1 (10 ng/ml), p-smad2/3 protein levels were examined by western blot analysis, and cellular apoptosis was detected by flow cytometry 6, 12, 24 and 48 h following tgf-β1 treatment. |
2014-02-13 |
2023-08-12 |
human |
| Yuan Huang, Fangjun Luo, Hui Li, Tao Jiang, Nong Zhan. Conditioned medium from alternatively activated macrophages induce mesangial cell apoptosis via the effect of Fas. Experimental cell research. vol 319. issue 19. 2014-02-13. PMID:23973665. |
the pro-apoptotic effect of m2 macrophages was demonstrated by condensed nuclei stained with hoechst 33258, increased apoptosis rates by flow cytometry analysis and enhanced caspase-3 activation by western blot. |
2014-02-13 |
2023-08-12 |
rat |
| Yifang Lu, Guogui Sun, Geling Liu, Yanping Shi, Ying Han, Fang Yu, Xiuxiu Xiang, Weijuan Li, Hongzhen Xiao, Xiuling Liu, Sha L. Clinical significance of mannose-binding lectin expression in thyroid carcinoma tissues. Pathology oncology research : POR. vol 19. issue 2. 2014-02-11. PMID:23250731. |
cell viability, apoptosis, rt-pcr and western blot assays were used to detect changes in tumor cell viability, apoptosis, and gene expression, respectively, after treatment of thyroid cancer cells with rhmbl. |
2014-02-11 |
2023-08-12 |
Not clear |
| S Zhang, Y Qi, H Zhang, W He, Q Zhou, S Gui, Y Wan. Melatonin inhibits cell growth and migration, but promotes apoptosis in gastric cancer cell line, SGC7901. Biotechnic & histochemistry : official publication of the Biological Stain Commission. vol 88. issue 6. 2014-02-10. PMID:23477595. |
we investigated the effects of melatonin on cell proliferation, apoptosis, colony formation and cell migration in the gastric adenocarcinoma cell line, sgc7901, using mtt assay, hoechst 33258 staining, flow cytometry, western blot, caspase-3 activity assay, soft agar colony formation assay, and scratch-wound assay. |
2014-02-10 |
2023-08-12 |
Not clear |
| Yasuaki Fukuda, Masahiro Kanbe, Manami Watanabe, Katsuaki Dan, Keiichi Matsuzaki, Susumu Kitanaka, Shohei Miyat. 3EZ,20Ac-ingenol, a catalytic inhibitor of topoisomerases, downregulates p-Akt and induces DSBs and apoptosis of DT40 cells. Archives of pharmacal research. vol 36. issue 8. 2014-02-04. PMID:23595550. |
western blot analysis revealed that 3ez,20ac-ingenol downregulated the expression of p-akt, and apoptosis was detected by the presence of dna double-strand breaks and caspase 3 activation. |
2014-02-04 |
2023-08-12 |
Not clear |
| H Karlsson, S C H Karlsson, A C Lindqvist, M Fransson, G Paul-Wetterberg, B Nilsson, M Essand, K Nilsson, P Frisk, H Jernberg-Wiklund, A Loskog, S I A Losko. Combining CAR T cells and the Bcl-2 family apoptosis inhibitor ABT-737 for treating B-cell malignancy. Cancer gene therapy. vol 20. issue 7. 2014-02-03. PMID:23788110. |
the car t cells were effective even if the cell lines exhibited different apoptosis resistance profiles, as shown by analyzing the expression of apoptosis inhibitors by pcr and western blot. |
2014-02-03 |
2023-08-12 |
Not clear |
| Qiuhuan Yuan, Shidou Zhao, Shangming Liu, Yanmin Zhang, Jie Fu, Fuwu Wang, Qian Liu, Eng-Ang Ling, Aijun Ha. Folic acid supplementation changes the fate of neural progenitors in mouse embryos of hyperglycemic and diabetic pregnancy. The Journal of nutritional biochemistry. vol 24. issue 7. 2014-01-29. PMID:23260036. |
the proliferation, apoptosis and differentiation of neural progenitors and neuronal migration were determined using brdu incorporation assay, tunel assay, immunofluorescence, western blot and real-time reverse transcriptase polymerase chain reaction. |
2014-01-29 |
2023-08-12 |
mouse |
| Liu Jinbo, Liu Zhiyuan, Zhang Zhijian, Ding WenG. Olfactory ensheathing cell-conditioned medium protects astrocytes exposed to hydrogen peroxide stress. Cellular and molecular neurobiology. vol 33. issue 5. 2014-01-29. PMID:23584685. |
the cell morphology was then observed under a light microscope, cell viability assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, cell ultrastructure observed with transmission electron microscopy (tem), and apoptosis assessed by annexin v staining followed by cytometry and western blot. |
2014-01-29 |
2023-08-12 |
Not clear |
| Yesol Bak, Sunyoung Ham, O Baatartsogt, Seung Hyun Jung, Kang-Duk Choi, Tae-Young Han, Il-Young Han, Do-Young Yoo. A1E inhibits proliferation and induces apoptosis in NCI-H460 lung cancer cells via extrinsic and intrinsic pathways. Molecular biology reports. vol 40. issue 7. 2014-01-29. PMID:23649764. |
the apoptosis was detected by nuclear morphological changes, annexin v-fitc/pi staining, cell cycle analysis, western blot, rt-pcr, and measurement of mitochondrial membrane potential. |
2014-01-29 |
2023-08-12 |
Not clear |
| Christina Pfister, Heike Pfrommer, Marcos S Tatagiba, Florian Rose. Detection and quantification of farnesol-induced apoptosis in difficult primary cell cultures by TaqMan protein assay. Apoptosis : an international journal on programmed cell death. vol 18. issue 4. 2014-01-24. PMID:23315006. |
apoptosis can be detected reliably by assaying for cleaved caspase-3, for which active caspase-3 antibodies are used in several methods, such as immunocytochemistry, enzyme-linked immunosorbent assay, and western blot. |
2014-01-24 |
2023-08-12 |
Not clear |
| Yongqiang Chen, Jinjuan Liu, Bo Yuan, Chengliang Cao, Sheng Qin, Xiaoying Cao, Guangkai Bian, Zhe Wang, Jihong Jian. Methylated actinomycin D, a novel actinomycin D analog induces apoptosis in HepG2 cells through Fas- and mitochondria-mediated pathways. Molecular carcinogenesis. vol 52. issue 12. 2014-01-23. PMID:22821714. |
semiquantitative rt-pcr and western blot analysis revealed that mact d induced apoptosis in hepg2 cells through mitochondria-dependent pathway by increasing levels of caspase-9, bax, bak while decreasing levels of bcl-2, bid, and fas-dependent pathway by increasing levels of fas, fasl, fadd, and caspase-8. |
2014-01-23 |
2023-08-12 |
human |
| Nabanita Patra, Umasankar De, Tae Hyung Kim, Young Ju Lee, Mee Young Ahn, Nam Deuk Kim, Jung Hyun Yoon, Wahn Soo Choi, Hyung Ryong Moon, Byung Mu Lee, Hyung Sik Ki. A novel histone deacetylase (HDAC) inhibitor MHY219 induces apoptosis via up-regulation of androgen receptor expression in human prostate cancer cells. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie. vol 67. issue 5. 2014-01-21. PMID:23583193. |
furthermore, mhy219 effectively increased apoptosis in du145 and lncap cells, but not pc3 cells, according to annexin v/pi staining and western blot analysis. |
2014-01-21 |
2023-08-12 |
human |
| Soon Young Shin, Hyuk Yoon, Seunghyun Ahn, Dong-Wook Kim, Sang Ho Kim, Dongsoo Koh, Young Han Lee, Yoongho Li. Chromenylchalcones showing cytotoxicity on human colon cancer cell lines and in silico docking with aurora kinases. Bioorganic & medicinal chemistry. vol 21. issue 14. 2014-01-21. PMID:23719279. |
further biological experiments including cell cycle analysis, apoptosis assay, western blot analysis, and immunofluorescent microscopy were carried out for this compound. |
2014-01-21 |
2023-08-12 |
human |
| Bei Jiang, Ling Guo, Bao-Ying Li, Jun-Hui Zhen, Jian Song, Tao Peng, Xiang-Dong Yang, Zhao Hu, Hai-Qing Ga. Resveratrol attenuates early diabetic nephropathy by down-regulating glutathione s-transferases Mu in diabetic rats. Journal of medicinal food. vol 16. issue 6. 2014-01-17. PMID:23767859. |
expressions of glutathione s-transferases mu (gstm) and nuclear factor erythroid 2-related factor 2 (nrf2) were detected by western blot, and apoptosis was analyzed using a flow cytometer. |
2014-01-17 |
2023-08-12 |
rat |
| Claudia Giampietri, Simonetta Petrungaro, Fabrizio Padula, Alessio D'Alessio, Elettra Sara Marini, Antonio Facchiano, Antonio Filippini, Elio Zipar. Autophagy modulators sensitize prostate epithelial cancer cell lines to TNF-alpha-dependent apoptosis. Apoptosis : an international journal on programmed cell death. vol 17. issue 11. 2014-01-10. PMID:22923157. |
cells were exposed to these molecules for 18, 24 and 48 h. autophagy was assessed via lc3 western blot analysis; propidium iodide and tunel stainings followed by flow cytometry or caspase-8 and caspase-3 activation assays were performed to evaluate apoptosis. |
2014-01-10 |
2023-08-12 |
Not clear |
| Hongbo Li, Baoying Wang, Chunhui Zhu, Yan Feng, Shaolan Wang, Muhammad Shahzad, Chenghu Hu, Mingshu Mo, Fangying Du, Xiaorui Y. 17β-estradiol impedes Bax-involved mitochondrial apoptosis of retinal nerve cells induced by oxidative damage via the phosphatidylinositol 3-kinase/Akt signal pathway. Journal of molecular neuroscience : MN. vol 50. issue 3. 2014-01-10. PMID:23361188. |
western blot and real-time pcr were used to determine the expression of the critical molecules in bcl-2 and caspase family associated with apoptosis. |
2014-01-10 |
2023-08-12 |
Not clear |
| Yueh-Shan Weng, Wei-Wen Kuo, Yueh-Min Lin, Chia-Hua Kuo, Bor-Show Tzang, Fuu-Jen Tsai, Chang-Hai Tsai, James A Lin, Dennis Jine-Yuan Hsieh, Chih-Yang Huan. Danshen mediates through estrogen receptors to activate Akt and inhibit apoptosis effect of Leu27IGF-II-induced IGF-II receptor signaling activation in cardiomyoblasts. Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association. vol 56. 2014-01-10. PMID:23419388. |
the danshen extract preventive effects on leu27igf-ii-induced igf-iir signaling activator and h9c2 cell apoptosis were identified using tunel assay, jc-1 staining and western blot assay. |
2014-01-10 |
2023-08-12 |
Not clear |
| Raimundo Fernandes de Araújo Júnior, Ana Luiza Cabral de Sá Leitão Oliveira, Jonas Bispo Pessoa, Vinícios Barreto Garcia, Gerlane Coelho Bernardo Guerra, Luiz Alberto Lira Soares, Tatiane Pereira de Souza, Pedro Ros Petrovick, Aurigena Antunes de Araúj. Maytenus ilicifolia dry extract protects normal cells, induces apoptosis and regulates Bcl-2 in human cancer cells. Experimental biology and medicine (Maywood, N.J.). vol 238. issue 11. 2014-01-10. PMID:23828591. |
cells were treated with sdemi for 4 and 24 h, then were assayed for levels of apoptosis, caspase-3, and bcl-2 by flow cytometry, immunostaining, and western blot, respectively. |
2014-01-10 |
2023-08-12 |
human |
| Hasan Riaz, Aixin Liang, Muhammad Kasib Khan, Ping Dong, Li Han, Muhammad Shahzad, Zhenlu Chong, Sibtain Ahmad, Guohua Hua, Liguo Yan. Somatostatin and its receptors: functional regulation in the development of mice Sertoli cells. The Journal of steroid biochemistry and molecular biology. vol 138. 2014-01-08. PMID:23831358. |
low (100pm and 10nm) and high dosage (1μm) groups of srif14 significantly promoted apoptosis, while all treatment groups led to dose dependent cessation (p<0.05) of g1 phase of cell cycle as further validated by increase in casp3, decrease in bcl2, elevation of p21 (all by western blot) and decrease in igf1 expressions, similarly, sst treatment caused a dose dependent suppression in the mrna level of kitl gene, which is important in the regulation of spermatogenesis. |
2014-01-08 |
2023-08-12 |
mouse |
| Wei Tian, Yongchuan Deng, Ling Li, Haifei He, Jie Sun, Dong X. Honokiol synergizes chemotherapy drugs in multidrug resistant breast cancer cells via enhanced apoptosis and additional programmed necrotic death. International journal of oncology. vol 42. issue 2. 2014-01-07. PMID:23242346. |
western blot analysis results proved that honokiol also enhanced vp-16-induced apoptosis potentially via blocking nuclear factor‑κb (nf-κb) activation. |
2014-01-07 |
2023-08-12 |
human |