| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Tiantian Tian, Jisheng Li, Bei Li, Yawei Wang, Meiying Li, Daoxin Ma, Xiuwen Wan. Genistein exhibits anti-cancer effects via down-regulating FoxM1 in H446 small-cell lung cancer cells. Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine. vol 35. issue 5. 2014-06-24. PMID:24379139. |
h446 cells were treated with various concentrations of genistein, and experiments including cck-8 assay, colony formation assay, flow cytometry analysis, wound healing assay, real-time polymerase chain reaction (pcr), western blot analysis, and plasmid transfection were used to investigate the influence of genistein on cell proliferation, migration ability, apoptosis, cell cycle progression, as well as the mrna and protein alterations of foxm1 pathway molecules. |
2014-06-24 |
2023-08-12 |
human |
| Minjun Wu, Hua Zhang, Jiehua Hu, Zhiyong Weng, Chenyuan Li, Hong Li, Yan Zhao, Xifan Mei, Fu Ren, Lihua L. Isoalantolactone inhibits UM-SCC-10A cell growth via cell cycle arrest and apoptosis induction. PloS one. vol 8. issue 9. 2014-06-16. PMID:24098753. |
to explain the molecular mechanisms underlying its effects, flow cytometry and western blot analysis showed that the apoptosis was associated with cell cycle arrest during the g1 phase, up-regulation of p53 and p21, and down-regulation of cyclin d. furthermore, our results revealed that induction of apoptosis through a mitochondrial pathway led to up-regulation of pro-apoptotic protein expression (bax), down-regulation of anti-apoptotic protein expression (bcl-2), mitochondrial release of cytochrome c (cyto c), reduction of mitochondrial membrane potential (mmp) and activation of caspase-3 (casp-3). |
2014-06-16 |
2023-08-12 |
Not clear |
| Jaejoon Lee, Hyemin Jeong, Eun-Jung Park, Ji Won Hwang, Bo Huang, Eun-Kyung Bae, Joong Kyong Ahn, Hoon-Suk Cha, Eun-Mi Ko. CIP2A facilitates apoptotic resistance of fibroblast-like synoviocytes in rheumatoid arthritis independent of c-Myc expression. Rheumatology international. vol 33. issue 9. 2014-06-13. PMID:23455633. |
to evaluate the signal transduction pathways engaged in apoptosis, caspase-3 activity, caspase-9 activity, parp, and phosphorylation of the akt kinase were analyzed by western blot. |
2014-06-13 |
2023-08-12 |
Not clear |
| Chuanyu Sun, Shuai Xu, Jia Guo, Ke Xu, Changchun Wan. The inhibitory and apoptotic effects of docetaxel-loaded mesoporous magnetic colloidal nanocrystal clusters on bladder cancer T24 cells in vitro. Journal of biomedical nanotechnology. vol 10. issue 3. 2014-06-12. PMID:24730241. |
a series of analyses for cell uptake, cell viability, cell cycle, cell apoptosis and some cell proteins were performed by transmission electron microscopy, mtt assay, flow cytometry, cell nuclei staining, annexin v staining assay, western blot assay and caspase-3 activity assay, respectively. |
2014-06-12 |
2023-08-13 |
Not clear |
| Jingjing Kong, Guiru Ren, Ning Jia, Yanfu Wang, Hua Zhang, Wei Zhang, Bingkun Chen, Yunpeng Ca. Effects of nicorandil in neuroprotective activation of PI3K/AKT pathways in a cellular model of Alzheimer's disease. European neurology. vol 70. issue 3-4. 2014-06-11. PMID:24008440. |
the cells were then collected for mtt, apoptosis assay, and western blot. |
2014-06-11 |
2023-08-12 |
Not clear |
| Wen-Jing Li, Shan-Liang Zhong, Yuan-Jian Wu, Wei-Dong Xu, Jin-Jin Xu, Jin-Hai Tang, Jian-Hua Zha. Systematic expression analysis of genes related to multidrug-resistance in isogenic docetaxel- and adriamycin-resistant breast cancer cell lines. Molecular biology reports. vol 40. issue 11. 2014-06-11. PMID:24078162. |
some key genes on the pathways related to drug resistance (including drug-transporters: mdr1, mrp1 and bcrp; drug metabolizing-enzymes: cyp3a4 and glutathione s-transferases (gst) pi; target genes: topoisomerase ii (topoiiα) and tubb3; apoptosis genes: bcl-2 and bax) were analyzed at rna and protein expression levels by real time rt-qpcr and western blot, respectively. |
2014-06-11 |
2023-08-12 |
human |
| Hao Li, Xin Xiang Li, Qing Ma, Jia Cu. The variability of oxLDL-induced cytotoxicity on different types of cell lines. Cell biochemistry and biophysics. vol 67. issue 2. 2014-06-07. PMID:23479334. |
huvec, k562/ao2 and ec-9706 cell lines were cultured in the presence of oxldl, and cell proliferation was tested by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (mtt) assay, apoptosis and cell cycle by flow cytometer, mrna expression by rt-pcr and protein expression by western blot. |
2014-06-07 |
2023-08-12 |
human |
| S Zahra Bathaie, Reyhane Hoshyar, Hamidreza Miri, Majid Sadeghizade. Anticancer effects of crocetin in both human adenocarcinoma gastric cancer cells and rat model of gastric cancer. Biochemistry and cell biology = Biochimie et biologie cellulaire. vol 91. issue 6. 2014-06-06. PMID:24219281. |
flow cytometry and caspases activity assays revealed apoptosis had been induced in these cells; rt-pcr and western blot analyses revealed the suppression of bcl-2 and up-regulation of bax expression in ags cells treated with crocetin. |
2014-06-06 |
2023-08-12 |
human |
| Liang Feng, Yan Chen, Ling Yuan, Xuan Liu, Jun-Fei Gu, Ming-Hua Zhang, Ying Wan. A combination of alkaloids and triterpenes of Alstonia scholaris (Linn.) R. Br. leaves enhances immunomodulatory activity in C57BL/6 mice and induces apoptosis in the A549 cell line. Molecules (Basel, Switzerland). vol 18. issue 11. 2014-06-06. PMID:24225772. |
western blot analysis also showed that their combination (2 mg raw material/kg) significantly down-regulated bcl-2 expression and pro-casp8 level, whereas it remarkably increased the level of cleaved caspase-8 leading to apoptosis in a549 cells. |
2014-06-06 |
2023-08-12 |
mouse |
| Xiongxiong Liu, Chao Sun, Xiaodong Jin, Ping Li, Fei Ye, Ting Zhao, Li Gong, Qiang L. Genistein enhances the radiosensitivity of breast cancer cells via G₂/M cell cycle arrest and apoptosis. Molecules (Basel, Switzerland). vol 18. issue 11. 2014-06-06. PMID:24284485. |
cell survival, dna damage and repair, cell cycle distribution, cell apoptosis, expression of proteins related to g₂/m cell cycle checkpoint and apoptosis were measured with colony formation assays, immunohistochemistry, flow cytometry and western blot analysis, respectively. |
2014-06-06 |
2023-08-12 |
human |
| b' Bircan Savran, Azmi Yerlikaya, Elif Erdo\\xc4\\x9fan, Osman Gen\\xc3\\xa. Anticancer agent ukrain and bortezomib combination is synergistic in 4T1 breast cancer cells. Anti-cancer agents in medicinal chemistry. vol 14. issue 3. 2014-05-30. PMID:23919744.' |
the induction of apoptosis was corroborated by the analysis of cleavage products of caspase-3 in 4t1 cells using western blot technique. |
2014-05-30 |
2023-08-12 |
Not clear |
| Aneta Rogalska, Arkadiusz Gajek, Agnieszka Marcza. Analysis of epothilone B-induced cell death in normal ovarian cells. Cell biology international. vol 37. issue 12. 2014-05-28. PMID:23955989. |
using fluorescence microscopy, polyacrylamide gel electrophoresis preceding western blot analysis, as well as spectrofluorimetric and colorimetric detection, we demonstrate that, compared to epob, ptx induced high time-dependent morphological and biochemical changes typical of apoptosis. |
2014-05-28 |
2023-08-12 |
Not clear |
| Baimei He, Bailing Luo, Qiong Chen, Lemeng Zhan. Cigarette smoke extract induces the expression of GRP78 in A549 cells via the p38/MAPK pathway. Molecular medicine reports. vol 8. issue 6. 2014-05-28. PMID:24126384. |
grp78 expression and its effect on the apoptosis of a549 cells were investigated using techniques such as rt-pcr, western blot analysis, gene knockdown by grp78 sirna interference and the terminal deoxynucleotidyl transferase dutp nick‑end labeling assay. |
2014-05-28 |
2023-08-12 |
Not clear |
| Bing Fang, Ming Zhang, Mai Tian, Lu Jiang, Hui Yuan Guo, Fa Zheng Re. Bovine lactoferrin binds oleic acid to form an anti-tumor complex similar to HAMLET. Biochimica et biophysica acta. vol 1841. issue 4. 2014-05-28. PMID:24368211. |
the anti-tumor activity was evaluated by methylene blue assay, while the apoptosis mechanism was analyzed using flow cytometry and western blot. |
2014-05-28 |
2023-08-12 |
cattle |
| M M Simon, J C Simard, D Girar. Viscum album agglutinin-I (VAA-I) increases cell surface expression of cytoskeletal proteins in apoptotic human neutrophils: moesin and ezrin are two novel targets of VAA-I. Human & experimental toxicology. vol 32. issue 10. 2014-05-27. PMID:23263853. |
in the present study, the expression and localization of ezrin and moesin by western blot and immunofluorescence revealed a clear degradation and relocalization of both the proteins during vaa-i-induced apoptosis. |
2014-05-27 |
2023-08-12 |
human |
| Shu-Kai Qiao, Han-Yun Ren, Yong-Jin Shi, Wei Li. Silencing HCCR2 expression inhibits the proliferation of leukemia cells by inducing apoptosis and promoting cell cycle arrest. International journal of molecular medicine. vol 32. issue 6. 2014-05-27. PMID:24126909. |
the expression levels of genes related to the cell cycle and apoptosis were detected by quantitative rt-pcr (qrt-pcr) and western blot analysis. |
2014-05-27 |
2023-08-12 |
human |
| Nan-Hyung Kim, Soo-Hyun Choi, Chang-Hyun Kim, Chang Hoon Lee, Tae Ryong Lee, Ai-Young Le. Reduced MiR-675 in exosome in H19 RNA-related melanogenesis via MITF as a direct target. The Journal of investigative dermatology. vol 134. issue 4. 2014-05-27. PMID:24335901. |
in western blot analysis, the mir-675 mimic reduced the expression of microphthalmia-associated transcription factor (mitf) and phosphorylation of camp-responsive element-binding protein, extracellular signal-regulated kinase and apoptosis signal-regulating kinase, whereas these expressions were increased by the mir-675 inhibitor. |
2014-05-27 |
2023-08-12 |
mouse |
| Basil A Smith, Corey L Neal, Mahandranauth Chetram, BaoHan Vo, Roman Mezencev, Cimona Hinton, Valerie A Odero-Mara. The phytoalexin camalexin mediates cytotoxicity towards aggressive prostate cancer cells via reactive oxygen species. Journal of natural medicines. vol 67. issue 3. 2014-05-22. PMID:23179315. |
increased caspase-3/7 activity and increased cleaved parp protein shown by western blot analysis was suggestive of increased apoptosis. |
2014-05-22 |
2023-08-12 |
Not clear |
| Kyoung-Jae Won, Joo-Young Im, Chae-Ok Yun, Kyung-Sook Chung, Young Joo Kim, Jung-Sun Lee, Young-Jin Jung, Bo-Kyung Kim, Kyung Bin Song, Young-Ho Kim, Ho-Kyung Chun, Kyeong Eun Jung, Moon-Hee Kim, Misun Wo. Human Noxin is an anti-apoptotic protein in response to DNA damage of A549 non-small cell lung carcinoma. International journal of cancer. vol 134. issue 11. 2014-05-22. PMID:24214091. |
the comet assay and western blot analysis revealed that hnoxin knockdown induced apoptosis through activation of p38 mitogen-activated protein kinase (mapk)/p53 in non-small cell lung carcinoma a549 cells. |
2014-05-22 |
2023-08-12 |
mouse |
| Siyan Chen, Chong Liu, Xiaoqian Wang, Xiujin Li, Yanling Chen, Nanhong Tan. 15-Deoxy-Δ(12,14)-prostaglandin J2 (15d-PGJ2) promotes apoptosis of HBx-positive liver cells. Chemico-biological interactions. vol 214. 2014-05-22. PMID:24582817. |
tetrazolium blue colorimetric method, flow cytometry, and western blot were performed to detect the proliferation, cycle, and apoptosis protein expression of hbx-positive hl7702 liver and control cells. |
2014-05-22 |
2023-08-12 |
Not clear |