| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| D Yin, S D Clarke, J L Peters, T D Etherto. Somatotropin-dependent decrease in fatty acid synthase mRNA abundance in 3T3-F442A adipocytes is the result of a decrease in both gene transcription and mRNA stability. The Biochemical journal. vol 331 ( Pt 3). 1998-10-26. PMID:9560309. |
insulin increased the abundance of fas mrna 2-13-fold and fatty acid synthesis 3-7-fold. |
1998-10-26 |
2023-08-12 |
rat |
| D Yin, S D Clarke, J L Peters, T D Etherto. Somatotropin-dependent decrease in fatty acid synthase mRNA abundance in 3T3-F442A adipocytes is the result of a decrease in both gene transcription and mRNA stability. The Biochemical journal. vol 331 ( Pt 3). 1998-10-26. PMID:9560309. |
somatotropin decreased the stimulatory effect of insulin on the abundance of fas mrna and lipogenesis by 40-70% and 20-60% respectively. |
1998-10-26 |
2023-08-12 |
rat |
| H S Sul, D Wan. Nutritional and hormonal regulation of enzymes in fat synthesis: studies of fatty acid synthase and mitochondrial glycerol-3-phosphate acyltransferase gene transcription. Annual review of nutrition. vol 18. 1998-10-22. PMID:9706228. |
insulin stimulates transcription of the fas and mitochondrial gpat genes, and glucagon antagonizes the insulin effect through the cis-acting elements within the promoters and their bound trans-acting factors. |
1998-10-22 |
2023-08-12 |
Not clear |
| D Wang, H S Su. Insulin stimulation of the fatty acid synthase promoter is mediated by the phosphatidylinositol 3-kinase pathway. Involvement of protein kinase B/Akt. The Journal of biological chemistry. vol 273. issue 39. 1998-10-15. PMID:9738010. |
we have shown that the rate of fas transcription is induced dramatically when fasted animals are refed with a high carbohydrate, fat-free diet or when streptozotocin-diabetic mice are given insulin. |
1998-10-15 |
2023-08-12 |
mouse |
| D Wang, H S Su. Insulin stimulation of the fatty acid synthase promoter is mediated by the phosphatidylinositol 3-kinase pathway. Involvement of protein kinase B/Akt. The Journal of biological chemistry. vol 273. issue 39. 1998-10-15. PMID:9738010. |
the fas promoter was up-regulated by insulin through the proximal insulin response sequence containing an e-box motif at the -65-base pair position. |
1998-10-15 |
2023-08-12 |
mouse |
| D Wang, H S Su. Insulin stimulation of the fatty acid synthase promoter is mediated by the phosphatidylinositol 3-kinase pathway. Involvement of protein kinase B/Akt. The Journal of biological chemistry. vol 273. issue 39. 1998-10-15. PMID:9738010. |
binding of upstream stimulatory factors to the -65 e-box is functionally required for insulin regulation of the fas promoter. |
1998-10-15 |
2023-08-12 |
mouse |
| D Wang, H S Su. Insulin stimulation of the fatty acid synthase promoter is mediated by the phosphatidylinositol 3-kinase pathway. Involvement of protein kinase B/Akt. The Journal of biological chemistry. vol 273. issue 39. 1998-10-15. PMID:9738010. |
in the present study, we characterized signaling pathways in the insulin stimulation of fas transcription using specific inhibitors for various signaling molecules and transfecting engineered phosphatidylinositol (pi) 3-kinase subunits and protein kinase b (pkb)/akt. |
1998-10-15 |
2023-08-12 |
mouse |
| D Wang, H S Su. Insulin stimulation of the fatty acid synthase promoter is mediated by the phosphatidylinositol 3-kinase pathway. Involvement of protein kinase B/Akt. The Journal of biological chemistry. vol 273. issue 39. 1998-10-15. PMID:9738010. |
as shown in rnase protection assays, ly294002 also inhibited insulin stimulation of the endogenous fas mrna levels in 3t3-l1 adipocytes. |
1998-10-15 |
2023-08-12 |
mouse |
| D Wang, H S Su. Insulin stimulation of the fatty acid synthase promoter is mediated by the phosphatidylinositol 3-kinase pathway. Involvement of protein kinase B/Akt. The Journal of biological chemistry. vol 273. issue 39. 1998-10-15. PMID:9738010. |
cotransfection of expression vectors for the constitutively active p110 subunit of pi 3-kinase resulted in an elevated fas promoter activity in the absence of insulin and a loss of further insulin stimulation. |
1998-10-15 |
2023-08-12 |
mouse |
| D Wang, H S Su. Insulin stimulation of the fatty acid synthase promoter is mediated by the phosphatidylinositol 3-kinase pathway. Involvement of protein kinase B/Akt. The Journal of biological chemistry. vol 273. issue 39. 1998-10-15. PMID:9738010. |
transfecting a dominant negative p85 subunit of pi 3-kinase decreased fas promoter activity and blocked insulin stimulation. |
1998-10-15 |
2023-08-12 |
mouse |
| D Wang, H S Su. Insulin stimulation of the fatty acid synthase promoter is mediated by the phosphatidylinositol 3-kinase pathway. Involvement of protein kinase B/Akt. The Journal of biological chemistry. vol 273. issue 39. 1998-10-15. PMID:9738010. |
furthermore, cotransfected wild-type pkb/akt increased fas promoter activity in the absence of insulin and a loss of insulin responsiveness of the fas promoter. |
1998-10-15 |
2023-08-12 |
mouse |
| D Wang, H S Su. Insulin stimulation of the fatty acid synthase promoter is mediated by the phosphatidylinositol 3-kinase pathway. Involvement of protein kinase B/Akt. The Journal of biological chemistry. vol 273. issue 39. 1998-10-15. PMID:9738010. |
on the other hand, kinase-dead pkb/akt acted in a dominant negative manner to decrease the fas promoter activity and abolished its insulin responsiveness. |
1998-10-15 |
2023-08-12 |
mouse |
| K J Claycombe, B H Jones, M K Standridge, Y Guo, J T Chun, J W Taylor, N Moustaïd-Mouss. Insulin increases fatty acid synthase gene transcription in human adipocytes. The American journal of physiology. vol 274. issue 5. 1998-06-24. PMID:9644037. |
the purpose of this study was to investigate the molecular mechanism whereby insulin increases expression of a key de novo lipogenic gene, fatty acid synthase (fas), in cultured human adipocytes and hepatoma cells. |
1998-06-24 |
2023-08-12 |
human |
| K J Claycombe, B H Jones, M K Standridge, Y Guo, J T Chun, J W Taylor, N Moustaïd-Mouss. Insulin increases fatty acid synthase gene transcription in human adipocytes. The American journal of physiology. vol 274. issue 5. 1998-06-24. PMID:9644037. |
in addition, run-on transcription assays and measurements of rna half-life were performed to determine the controlled step in fas gene regulation by insulin. |
1998-06-24 |
2023-08-12 |
human |
| K J Claycombe, B H Jones, M K Standridge, Y Guo, J T Chun, J W Taylor, N Moustaïd-Mouss. Insulin increases fatty acid synthase gene transcription in human adipocytes. The American journal of physiology. vol 274. issue 5. 1998-06-24. PMID:9644037. |
insulin induced an approximately five- and three-fold increase in fas mrna content in adipocytes and hepatoma cells, respectively. |
1998-06-24 |
2023-08-12 |
human |
| K J Claycombe, B H Jones, M K Standridge, Y Guo, J T Chun, J W Taylor, N Moustaïd-Mouss. Insulin increases fatty acid synthase gene transcription in human adipocytes. The American journal of physiology. vol 274. issue 5. 1998-06-24. PMID:9644037. |
furthermore, we demonstrated that the induction of human fas expression by insulin was due to increased transcription rate of the fas gene in human adipocytes, whereas mrna stabilization accounted for increased fas mrna content in hepatoma cells. |
1998-06-24 |
2023-08-12 |
human |
| K J Claycombe, B H Jones, M K Standridge, Y Guo, J T Chun, J W Taylor, N Moustaïd-Mouss. Insulin increases fatty acid synthase gene transcription in human adipocytes. The American journal of physiology. vol 274. issue 5. 1998-06-24. PMID:9644037. |
in conclusion, we report here for the first time expression of human fas mrna and its specific transcriptional induction by insulin in cultured human adipocytes. |
1998-06-24 |
2023-08-12 |
human |
| J B Kim, P Sarraf, M Wright, K M Yao, E Mueller, G Solanes, B B Lowell, B M Spiegelma. Nutritional and insulin regulation of fatty acid synthetase and leptin gene expression through ADD1/SREBP1. The Journal of clinical investigation. vol 101. issue 1. 1998-02-09. PMID:9421459. |
this elevation of add1/srebp1, leptin, and fas that is induced by feeding in vivo is mimicked by exposure of cultured adipocytes to insulin, the classic hormone of the fed state. |
1998-02-09 |
2023-08-12 |
Not clear |
| J B Kim, P Sarraf, M Wright, K M Yao, E Mueller, G Solanes, B B Lowell, B M Spiegelma. Nutritional and insulin regulation of fatty acid synthetase and leptin gene expression through ADD1/SREBP1. The Journal of clinical investigation. vol 101. issue 1. 1998-02-09. PMID:9421459. |
molecular dissection of the fas promoter shows that most if not all of this action of add1/srebp1 is through an e-box motif at -64 to -59, contained with a sequence identified previously as the major insulin response element of this gene. |
1998-02-09 |
2023-08-12 |
Not clear |
| D Wang, H S Su. Upstream stimulatory factor binding to the E-box at -65 is required for insulin regulation of the fatty acid synthase promoter. The Journal of biological chemistry. vol 272. issue 42. 1997-11-17. PMID:9334210. |
we have shown that fas transcription rate is induced dramatically when fasted animals are refed with a high carbohydrate diet or when streptozotocin-diabetic mice are given insulin. |
1997-11-17 |
2023-08-12 |
mouse |