| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Fang Zeng, Yun Cheng Li, Gang Chen, Yong Kui Zhang, Ye Kai Wang, Shi Quan Zhou, Li Na Ma, Ji Hang Zhou, Yan Yan Huang, Wang Yu Zhu, Xiao Guang Li. Nicotine inhibits cisplatin-induced apoptosis in NCI-H446 cells. Medical oncology (Northwood, London, England). vol 29. issue 1. 2012-08-23. PMID:21267677. |
an mtt assay, annexin v-fitc staining, rt-pcr, and western blot were applied to identify the viability of cells, stages of apoptosis, mrna and signaling proteins expression, respectively. |
2012-08-23 |
2023-08-12 |
Not clear |
| Tzuu-Yuan Huang, Che-Wen Hsu, Weng-Cheng Chang, Miin-Yau Wang, June-Fu Wu, Yi-Chiang Hs. Demethoxycurcumin Retards Cell Growth and Induces Apoptosis in Human Brain Malignant Glioma GBM 8401 Cells. Evidence-based complementary and alternative medicine : eCAM. vol 2012. 2012-08-23. PMID:22454662. |
in the present study, the antiproliferation (using the mtt assay, dmc was found to have cytotoxic activities against gbm 8401 cell with ic(50) values at 22.71 μm) and induced apoptosis effects of dmc have been investigated in human brain malignant glioma gbm 8401 cells. |
2012-08-23 |
2023-08-12 |
human |
| Xiao-Chun Wang, Yi-Hua Qiu, Yu-Ping Pen. Interleukin-6 protects cerebellar granule neurons from NMDA-induced neurotoxicity. Sheng li xue bao : [Acta physiologica Sinica]. vol 59. issue 2. 2012-08-20. PMID:17437036. |
methyl-thiazole-tetrazolium (mtt) assay, terminal deoxynucleotidyl transferase-mediated dutp nick end labeling (tunel) method and confocal laser scanning microscope (clsm) were used to detect neuronal vitality, apoptosis and dynamic changes of intracellular ca(2+) levels in the neurons, respectively. |
2012-08-20 |
2023-08-12 |
rat |
| A Anitha, K P Chennazhi, S V Nair, R Jayakuma. 5-flourouracil loaded N,O-carboxymethyl chitosan nanoparticles as an anticancer nanomedicine for breast cancer. Journal of biomedical nanotechnology. vol 8. issue 1. 2012-08-16. PMID:22515092. |
results of anticancer activity via mtt, apoptosis and caspase 3 assays showed the toxicity of the drug loaded nanoparticles towards breast cancer cells. |
2012-08-16 |
2023-08-12 |
Not clear |
| Zufeng Ding, Shijie Liu, Bo Yang, Yubo Fan, Xiaoyan Den. Effect of oxidized low-density lipoprotein concentration polarization on human smooth muscle cells' proliferation, cycle, apoptosis and oxidized low-density lipoprotein uptake. Journal of the Royal Society, Interface. vol 9. issue 71. 2012-08-15. PMID:22048945. |
to clarify the effect of concentration polarization of oxidative modification of low-density lipoproteins (ox-ldls) on human smooth muscle cells (smcs), the proliferation, ox-ldl uptake and apoptosis with smcs cultured on permeable (the permeable group) or non-permeable membranes (the non-permeable group) were analysed by 3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide (mtt) assay, spectrofluorometry and flow cytometry using a parallel-plate flow chamber technique. |
2012-08-15 |
2023-08-12 |
human |
| Bingfang Wang, Xuewu Zhan. Inhibitory effects of Broccolini leaf flavonoids on human cancer cells. Scanning. vol 34. issue 1. 2012-08-14. PMID:22532078. |
cell growth inhibition was evaluated using a standard colorimetric mtt assay, cellular morphology was observed using phase contrast microscopy and flow cytometry was introduced to further investigate cells apoptosis effect. |
2012-08-14 |
2023-08-12 |
human |
| Ming Wu, Yuan-gui Zhu, Xiao-dong Pan, Nan Lin, Jing Zhang, Xiao-chun Che. [Involvement of Wnt/beta-catenin signaling in tripchlorolide protecting against oligomeric beta-amyloid-(1-42)-induced neuronal apoptosis]. Yao xue xue bao = Acta pharmaceutica Sinica. vol 45. issue 7. 2012-08-13. PMID:20931782. |
then the cell viability, neuronal apoptosis, and protein levels of wnt, glycogen synthase kinase 3beta (gsk3beta), beta-catenin and phospho-beta-catenin were measured by mtt assay, tunel staining and western blotting, respectively. |
2012-08-13 |
2023-08-12 |
Not clear |
| Yanli Ge, Junjie Zhang, Jianchun Cao, Qiong Wu, Longe Sun, Likun Guo, Zhirong Wan. TFF1 inhibits proliferation and induces apoptosis of gastric cancer cells in vitro. Bosnian journal of basic medical sciences. vol 12. issue 2. 2012-08-13. PMID:22642590. |
the cell proliferation was detected with mtt assay and apoptosis and cell cycle measured by flow cytometry. |
2012-08-13 |
2023-08-12 |
Not clear |
| Rui Wang, Yi Hu, Ge Song, Chan Juan Hao, Yi Cui, Hong-Fei Xia, Xu M. MiR-206 regulates neural cells proliferation and apoptosis via Otx2. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology. vol 29. issue 3-4. 2012-08-09. PMID:22508046. |
ectopic expression of mir-206 inhibited cell proliferation, and promoted cell apoptosis in u343 and sk-n-sh cell by using mtt, edu apollo assay and flow cytometry analysis. |
2012-08-09 |
2023-08-12 |
chicken |
| A H Daneshmanesh, M Hojjat-Farsangi, A S Khan, M Jeddi-Tehrani, M M Akhondi, A A Bayat, R Ghods, A-R Mahmoudi, R Hadavi, A Österborg, F Shokri, H Rabbani, H Mellsted. Monoclonal antibodies against ROR1 induce apoptosis of chronic lymphocytic leukemia (CLL) cells. Leukemia. vol 26. issue 6. 2012-08-07. PMID:22289919. |
all five mabs alone induced apoptosis in the absence of complement or added effector cells (annexin-v and mtt, as well as cleavage of poly-(adp ribose)-polymerase, caspase-8 and caspase-9) of cll cells but not of normal b cells. |
2012-08-07 |
2023-08-12 |
human |
| Hao Zhu, Ming-Feng Chen, Wen-Juan Yu, Wen-Jin Wang, Feng Li, Wen-Chao Liu, Yan Wo, Rong Xia, Wen-Long Din. Time-dependent changes in BDNF expression of pentylenetetrazole-induced hippocampal astrocytes in vitro. Brain research. vol 1439. 2012-07-30. PMID:22265706. |
cells were incubated with 10, 20, or 40 mm ptz for 24h and viability and apoptosis were examined using an mtt assay and hoechst staining. |
2012-07-30 |
2023-08-12 |
Not clear |
| Hao Zhu, Ming-Feng Chen, Wen-Juan Yu, Wen-Jin Wang, Feng Li, Wen-Chao Liu, Yan Wo, Rong Xia, Wen-Long Din. Time-dependent changes in BDNF expression of pentylenetetrazole-induced hippocampal astrocytes in vitro. Brain research. vol 1439. 2012-07-30. PMID:22265706. |
the high concentration of ptz (20 and 40 mm) resulted in a significant decrease in viability (mtt: 83.6 ± 7.8% and 69.3 ± 4.2%, respectively) (p<0.01), whereas the lower concentration of ptz (10mm) did not induce cell apoptosis or reduce viability. |
2012-07-30 |
2023-08-12 |
Not clear |
| Young Joo Choi, Yang Jee Kim, Joong Won Lee, Younghyun Lee, Yong-Beom Lim, Hai Won Chun. Cyto-/genotoxic effect of CdSe/ZnS quantum dots in human lung adenocarcinoma cells for potential photodynamic UV therapy applications. Journal of nanoscience and nanotechnology. vol 12. issue 3. 2012-07-26. PMID:22755033. |
qd-induced cell death was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (mtt), apoptosis, and lactate dehydrogenase (ldh) assays, as well as by real-time pcr analysis of differential mrna levels of genes, such as ataxia telangiectasia mutated (atm), p53, and caspase-9, involved in apoptosis. |
2012-07-26 |
2023-08-12 |
human |
| Bin Li, Zhiming Wu, Wenzhi Li, Guojin Jia, Jiancheng Lu, Jie Fang, Gang Che. Chlorophyllin e4 is a novel photosensitizer against human bladder cancer cells. Oncology reports. vol 27. issue 5. 2012-07-23. PMID:22294235. |
photocytotoxicity was monitored with mtt assay and apoptosis was measured by flow cytometry. |
2012-07-23 |
2023-08-12 |
human |
| S Kim, H Youn, M G Song, J H Kang, H K Chung, D S Lee, J-K Chun. Complementary treatment of siTERT for improving the antitumor effect of TERT-specific I-131 therapy. Cancer gene therapy. vol 19. issue 4. 2012-07-20. PMID:22301953. |
cell proliferation was monitored by mtt assay and induced apoptosis was confirmed by annexin-v-pi staining. |
2012-07-20 |
2023-08-12 |
mouse |
| Ru-Ming Liu, Ying-Bo Li, Jian-Jiang Zhon. Cytotoxic and pro-apoptotic effects of novel ganoderic acid derivatives on human cervical cancer cells in vitro. European journal of pharmacology. vol 681. issue 1-3. 2012-07-20. PMID:22366428. |
mtt assay indicated that, among the tested compounds, tlto-a displayed the highest inhibitory effect on the growth of hela cells, whereas it showed less cytotoxicity to the non-tumorous cell line mcf-10a than ganoderic acid t. flow cytometry analysis revealed that all the compounds caused cell cycle arrest at the g1 phase and induced apoptosis. |
2012-07-20 |
2023-08-12 |
human |
| Jeong-Dan Cha, Ji-Young Ki. Essential oil from Cryptomeria japonica induces apoptosis in human oral epidermoid carcinoma cells via mitochondrial stress and activation of caspases. Molecules (Basel, Switzerland). vol 17. issue 4. 2012-07-19. PMID:22466850. |
we examined the cell viability and apoptosis of kb cells treated with c. japonica essential oil at several concentrations for 12 h by mtt assay, hoechst-33258 dye staining, dna fragmentation, flow cytometry (cell cycle), and western blotting for mitochondria stress, activation of caspases, and poly (adp-ribose) polymerase. |
2012-07-19 |
2023-08-12 |
human |
| Gui-Rong Yu, Wei-Wei Qin, Ji-Peng Li, Wei Hua, Yan-Ling Meng, Rui Chen, Bo Yan, Lei Wang, Xiang Zhang, Lin-Tao Jia, Jing Zhao, Rui Zhang, An-Gang Yan. HIV-TAT-fused FHIT protein functions as a potential pro-apoptotic molecule in hepatocellular carcinoma cells. Bioscience reports. vol 32. issue 3. 2012-07-16. PMID:21679157. |
furthermore, the results of mtt [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2h-tetrazolium bromide] assay, annexin-v staining and western blotting demonstrated that tat-fhit can robustly inhibit growth and induce apoptosis of hcc cells in vitro. |
2012-07-16 |
2023-08-12 |
human |
| Kuiran Liu, Tianda Feng, Jie Liu, Ming Zhong, Shulan Zhan. Silencing of the DEK gene induces apoptosis and senescence in CaSki cervical carcinoma cells via the up-regulation of NF-κB p65. Bioscience reports. vol 32. issue 3. 2012-07-16. PMID:22390170. |
the proliferation activity of cells were measured using an mtt [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2h-tetrazolium bromide] assay, cell apoptosis was measured using an annexin v/pi (propidium iodide) kit, the cell cycle was analysed by flow cytometry and cell senescence was detected using senescence β-galactosidase staining. |
2012-07-16 |
2023-08-12 |
human |
| Ya-Xin Han, De-Yong Lian. The role of the tumor suppressor RUNX3 in giant cell tumor of the bone. International journal of oncology. vol 40. issue 3. 2012-07-03. PMID:22076387. |
in in vitro experiments, exogenous expression of runx3 strongly inhibited cell growth in gctb by mtt (p<0.05), induced apoptosis as evidenced by annexin v-fitc and increased g1 phase ratio by pi (p<0.05). |
2012-07-03 |
2023-08-12 |
human |