| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Reyhaan A Chaudhri, Rene Olivares-Navarrete, Natalia Cuenca, Agreen Hadadi, Barbara D Boyan, Zvi Schwart. Membrane estrogen signaling enhances tumorigenesis and metastatic potential of breast cancer cells via estrogen receptor-α36 (ERα36). The Journal of biological chemistry. vol 287. issue 10. 2012-04-24. PMID:22247547. |
cell proliferation was determined by [(3)h]thymidine incorporation, and cell viability was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, (mtt) whereas apoptosis was determined by dna fragmentation and tunel. |
2012-04-24 |
2023-08-12 |
Not clear |
| Song-qiang Xie, Qian Li, Hong-xia Ma, Ya-hong Zhang, Jian-hong Wang, Jin Zhao, Chao-jie Wan. [Apoptotic mechanism of WJH-6, a novel polyamine conjugate, on K562 and HL-60 cells]. Yao xue xue bao = Acta pharmaceutica Sinica. vol 45. issue 4. 2012-04-16. PMID:21355208. |
the cytotoxicity of wjh-6 was assessed by mtt assay; cell cycle distribution and apoptosis were measured by flow cytometry; the protein expression of caspase-3, caspase-8, caspase-9, bid and mitochondrial membrane potential (mmp) were evaluated by high content screening (hcs) analysis; the protein expression of cytochrome c was measured by western blotting. |
2012-04-16 |
2023-08-12 |
Not clear |
| Jian Chen, Dong-Yun Shi, Shan-Lin Liu, Liang Zhon. Tanshinone IIA induces growth inhibition and apoptosis in gastric cancer in vitro and in vivo. Oncology reports. vol 27. issue 2. 2012-04-05. PMID:22038415. |
cell viability and apoptosis in vitro were evaluated through the mtt assay and flow cytometry analysis. |
2012-04-05 |
2023-08-12 |
mouse |
| Yanjing Yang, Xuewu Zhan. In vitro antitumor activity of broccolini seeds extracts. Scanning. vol 33. issue 6. 2012-04-04. PMID:21695707. |
it was found that bse induces a549 and ovcar-3 cells apoptosis in a dose-dependent manner by using mtt assay. |
2012-04-04 |
2023-08-12 |
human |
| Li Zhu, Hui Yuan, Chengzhi Guo, Yin Lu, Sijun Deng, Yang Yang, Qiang Wei, Lixin Wen, Zuping H. Zearalenone induces apoptosis and necrosis in porcine granulosa cells via a caspase-3- and caspase-9-dependent mitochondrial signaling pathway. Journal of cellular physiology. vol 227. issue 5. 2012-04-02. PMID:21732350. |
we found that zearalenone reduced the proliferation of porcine granulosa cells in a dose-dependent manner as shown by the mtt assay and zearalenone resulted in an obvious apoptosis and necrosis in porcine granulosa cells as determined by the tunel analysis and flow cytometry. |
2012-04-02 |
2023-08-12 |
Not clear |
| Zhi-Kai Dai, Jiang-Ke Qin, Jiao-E Huang, Yong Luo, Qing Xu, Hai-Lu Zha. Tanshinone IIA activates calcium-dependent apoptosis signaling pathway in human hepatoma cells. Journal of natural medicines. vol 66. issue 1. 2012-04-02. PMID:21853384. |
human hepatoma bel-7402 cells treated with tan iia underwent assessment with mtt assay for cell viability, 10-day culture for colony formation, flow cytometry and fluorescence microscopy for apoptosis and cell cycle analysis. |
2012-04-02 |
2023-08-12 |
human |
| Dong-Yan Shen, Jin-He Kang, Wei Song, Wen-Qing Zhang, Wen-Gang Li, Yan Zhao, Qing-Xi Che. Apoptosis of human cholangiocarcinoma cell lines induced by β-escin through mitochondrial caspase-dependent pathway. Phytotherapy research : PTR. vol 25. issue 10. 2012-03-27. PMID:21394804. |
cell growth, cell cycle and apoptosis were investigated, respectively, by mtt assay, single pi and fitc/pi double-staining flow cytometry, and fluorescence microscopy. |
2012-03-27 |
2023-08-12 |
human |
| Yongming Sun, Haibin Wang, Fanguo Lin, Jun Hua, Gaoli Zho. Inhibition of proliferation and gene expression regulation by (-)-epigallocatechin-3-gallate in human synovial sarcoma cells. Medical oncology (Northwood, London, England). vol 28. issue 4. 2012-03-22. PMID:20480267. |
the mtt assay and dapi staining indicated that egcg effectively inhibited cellular proliferation and induces apoptosis of the synovial sarcoma cells and induced apoptosis as confirmed by flow cytometry. |
2012-03-22 |
2023-08-12 |
human |
| Runjun Yang, Shangzhong Xu, Zhihui Zhao, Junya L. Fas ligand expression and mediated activation of an apoptosis program in bovine follicular granulosa cells. Gene. vol 493. issue 1. 2012-03-22. PMID:22155318. |
the methyl-tetrazolium (mtt) assay, hoechst33342 staining, and dna ladder method were performed to determine the growth inhibition and apoptosis of the cells. |
2012-03-22 |
2023-08-12 |
cattle |
| Jie Yin, Hui Fang Tang, Qiong Xiang, Jia Yu, Xiao Yang Yang, Nan Hu, Xiao Yong Le. MiR-122 increases sensitivity of drug-resistant BEL-7402/5-FU cells to 5-fluorouracil via down-regulation of bcl-2 family proteins. Die Pharmazie. vol 66. issue 12. 2012-03-20. PMID:22312705. |
accordingly, p53 protein expression showed a significant up-regulation; mtt results showed that after incubation with 5-fu, mir-122 transfectants had higher cell inhibitory rates than negative mirna or untreated cells; flow cytometry results demonstrated that apoptosis rate increased in mir-122 transfected cells, compared with negative mirna or untreated cells. |
2012-03-20 |
2023-08-12 |
human |
| Elena B Burova, Irina S Smirnova, Ilya V Gonchar, Alla N Shatrova, Nikolay N Nikolsk. Inhibition of the EGF receptor and ERK1/2 signaling pathways rescues the human epidermoid carcinoma A431 cells from IFNγ-induced apoptosis. Cell cycle (Georgetown, Tex.). vol 10. issue 13. 2012-03-16. PMID:21606674. |
based on the data provided by evaluating cell morphology, mtt assay, facs analysis, and cleaved caspase-3 staining we concluded that the major cause of ifnγ-induced a431 cell growth inhibition was not cell cycle arrest, but apoptosis. |
2012-03-16 |
2023-08-12 |
human |
| Yuqin Li, Bochao Zhang, Xiaoqin Wang, Huidan Yan, Gu Chen, Xuewu Zhan. Proteomic analysis of apoptosis induction in human lung cancer cells by recombinant MVL. Amino acids. vol 41. issue 4. 2012-03-01. PMID:21069400. |
we found that r-mvl induces a549 cells apoptosis in a dose-dependent manner by using mtt assay, fluorescent microscope (fm) and flow cytometry (fcm), and the ic50 was calculated to be 24.12 μg/ml. |
2012-03-01 |
2023-08-12 |
human |
| Tie-Qiang Bi, Xiang-Ming Che, Xin-Hua Liao, Dan-Jie Zhang, Hou-Long Long, Hai-Jun Li, Wei Zha. Overexpression of Nampt in gastric cancer and chemopotentiating effects of the Nampt inhibitor FK866 in combination with fluorouracil. Oncology reports. vol 26. issue 5. 2012-03-01. PMID:21743967. |
as determined by mtt assay and flow cytometry, fk866 also increased the chemo-sensitivity of gastric cancer cells to fluorouracil by greater inhibition of cell proliferation and the induction of apoptosis. |
2012-03-01 |
2023-08-12 |
human |
| Lifang Wang, An Lu, Xiaoxia Liu, Meixiang Sang, Baoen Shan, Fanru Meng, Qing Cao, Xin J. The flavonoid Baohuoside-I inhibits cell growth and downregulates survivin and cyclin D1 expression in esophageal carcinoma via β-catenin-dependent signaling. Oncology reports. vol 26. issue 5. 2012-03-01. PMID:21785828. |
the growth inhibitory effect of baohuoside-i on the eca109 tumor cell line was examined by mtt assay; the induction of apoptosis was analyzed by flow cytometry. |
2012-03-01 |
2023-08-12 |
mouse |
| Shudong Yu, Ruxin Zhang, Fenye Liu, Hua Hu, Shaoqing Yu, Hong Wan. Down-regulation of Notch signaling by a γ-secretase inhibitor enhances the radiosensitivity of nasopharyngeal carcinoma cells. Oncology reports. vol 26. issue 5. 2012-03-01. PMID:21805038. |
in the present study, radiation-induced anti-proliferative effects of the one gsi (n-[(3,5-difluorophenyl)acetyl]-l-alanyl-2-phenyl]glycine-1,1-dimethylethyl ester, dapt), on cne2 cells were investigated with the mtt assay; in vitro radiosensitization effects were evaluated by the apoptosis assay and the cell colony formation assay. |
2012-03-01 |
2023-08-12 |
Not clear |
| Masanori Horie, Haruhisa Kato, Shigehisa Endoh, Katsuhide Fujita, Keiko Nishio, Lilian Kaede Komaba, Hiroko Fukui, Ayako Nakamura, Arisa Miyauchi, Tetsuya Nakazato, Shinichi Kinugasa, Yasukazu Yoshida, Yoshihisa Hagihara, Yasuo Morimoto, Hitoshi Iwahash. Evaluation of cellular influences of platinum nanoparticles by stable medium dispersion. Metallomics : integrated biometal science. vol 3. issue 11. 2012-02-29. PMID:21804981. |
cell viability (mtt assay), cell proliferation (clonogenic assay), apoptosis induction (caspase-3 activity), intracellular ros level (dcfh assay), and lipid peroxidation level (dppp assay) were measured as markers of cellular influences. |
2012-02-29 |
2023-08-12 |
Not clear |
| Chun Ying Xie, Wei Yang, Jun Ying, Qing Chun Ni, Xue Diao Pan, Jin Hua Dong, Karen Li, Xi Sha Wan. B-cell lymphoma-2 over-expression protects δ-elemene-induced apoptosis in human lung carcinoma mucoepidermoid cells via a nuclear factor kappa B-related pathway. Biological & pharmaceutical bulletin. vol 34. issue 8. 2012-02-27. PMID:21804218. |
the result demonstrated that δ-elemene significantly induced apoptosis of nci-h292, as shown by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (mtt) assay, dna fragmentation measurement, annexin v (anv) binding of externalized phosphatidylserine and the mitochondrial probe jc-1 using flow cytometry. |
2012-02-27 |
2023-08-12 |
human |
| Ruitao Zhang, Huirong Shi, Zhimin Chen, Wei Feng, Hailing Zhang, Kaiyuan W. Effects of kallikrein-related peptidase 14 gene inhibition by small interfering RNA in ovarian carcinoma cells. Molecular medicine reports. vol 5. issue 1. 2012-02-27. PMID:21993700. |
cell proliferation, apoptosis and invasion were examined by mtt, flow cytometry and matrigel assay, respectively. |
2012-02-27 |
2023-08-12 |
Not clear |
| Jun Ying, Wei Yang, Chun-Ying Xie, Qin-Chun Ni, Xue-Diao Pan, Jin-Hua Dong, Zhi-Min Liu, Xi-Sha Wan. Induction of caspase-3-dependent apoptosis in human leukemia HL-60 cells by δ-elemene. Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan. vol 131. issue 9. 2012-02-24. PMID:21881314. |
the results demonstrated that δ-elemene induces significant apoptosis of hl-60 cells, as shown by mtt assay, annexin v (anv) binding of externalized phosphatidylserine (ps), and the mitochondrial probe jc-1 using flow cytometry. |
2012-02-24 |
2023-08-12 |
human |
| Shuang Zhang, Hong Li, Hui Xu, Shi-Jie Yan. [Effect of gross saponins of Tribulus terrestris on cardiocytes impaired by adriamycin]. Yao xue xue bao = Acta pharmaceutica Sinica. vol 45. issue 1. 2012-02-23. PMID:21351446. |
mtt colorimetric method was deployed to detect cardiocyte survival rate, activities of ck, ldh, ast, sod, mda and no were detected, and apoptosis was detected with flow cytometry. |
2012-02-23 |
2023-08-12 |
rat |