| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| He Wang, Yongli Shi, Danielle Major, Zhanjun Yan. Lung epithelial cell death induced by oil-dispersant mixtures. Toxicology in vitro : an international journal published in association with BIBRA. vol 26. issue 5. 2012-10-02. PMID:22504303. |
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide mtt assay, lactate dehydrogenase assay, morphology and cleaved caspase 9 protein, and microtubule-associated protein 1 light chain 3 were all used to measure cell viability, necrosis, apoptosis and autophagy quantitation, respectively. |
2012-10-02 |
2023-08-12 |
Not clear |
| F Rong, W Li, K Chen, D M Li, W M Duan, Y Z Feng, F Li, X W Zhou, S J Fan, Y Liu, M Ta. Knockdown of RhoGDIα induces apoptosis and increases lung cancer cell chemosensitivity to paclitaxel. Neoplasma. vol 59. issue 5. 2012-09-27. PMID:22668020. |
apoptosis was determined by flow cytometric assessment, and cell viability was measured with the mtt assay. |
2012-09-27 |
2023-08-12 |
Not clear |
| Danielle F Mello, Eliza S de Oliveira, Renato C Vieira, Erik Simoes, Rafael Trevisan, Alcir Luiz Dafre, Margherita Anna Barracc. Cellular and transcriptional responses of Crassostrea gigas hemocytes exposed in vitro to brevetoxin (PbTx-2). Marine drugs. vol 10. issue 3. 2012-09-24. PMID:22611355. |
hemocyte viability, monitored through the neutral red retention and mtt reduction assays, and apoptosis (hoechst staining) remained unchanged during 12 h of exposure to pbtx-2 in concentrations up to 1000 µg/l. |
2012-09-24 |
2023-08-12 |
Not clear |
| Jie Cao, Dong-Ling Dai, Long Yao, Hui-Hong Yu, Bo Ning, Qin Zhang, Juan Chen, Wen-Hui Cheng, Wei Shen, Zhao-Xia Yan. Saturated fatty acid induction of endoplasmic reticulum stress and apoptosis in human liver cells via the PERK/ATF4/CHOP signaling pathway. Molecular and cellular biochemistry. vol 364. issue 1-2. 2012-09-20. PMID:22246806. |
mtt assay was used for cell viability, flow cytometry and hoechst 33258 staining for apoptosis, rt-pcr for mrna expression, and western blot for protein expression. |
2012-09-20 |
2023-08-12 |
human |
| Zu-An Zhu, Zheng-Qiu Zhu, Hong-Xing Cai, Ying Li. Reversion of multidrug resistance by SKI-II in SGC7901/DDP cells and exploration of underlying mechanisms. Asian Pacific journal of cancer prevention : APJCP. vol 13. issue 2. 2012-09-20. PMID:22524836. |
then cell growth, apoptosis, micro- morphological changes, and expression of sphk1, p-gp, nf-kb, bcl-2 and bax were assessed by mtt assay, flow cytometry, electron microscopy, immunocytochemistry and western blot assay respectively. |
2012-09-20 |
2023-08-12 |
Not clear |
| Xiaoliang Wang, Chongzhi Zhou, Guoqiang Qiu, Yinxue Yang, Dongwang Yan, Tonghai Xing, Junwei Fan, Huamei Tang, Zhihai Pen. Phospholipase C epsilon plays a suppressive role in incidence of colorectal cancer. Medical oncology (Northwood, London, England). vol 29. issue 2. 2012-09-18. PMID:21667163. |
the effect of plce1 overexpression on cancer cell malignant degree was measured by mtt assay, plate colony formation assay, soft agar colony formation assay, cell cycle and apoptosis analysis, and xenograft assay. |
2012-09-18 |
2023-08-12 |
Not clear |
| Chengzhen Liang, Hao Li, Chengchun Shen, Jianbo Lai, Zhongli Shi, Bing Liu, Hui-min Ta. Genistein potentiates the anti-cancer effects of gemcitabine in human osteosarcoma via the downregulation of Akt and nuclear factor-κB pathway. Anti-cancer agents in medicinal chemistry. vol 12. issue 5. 2012-09-17. PMID:22263786. |
in vitro, by mtt, electron microscopy, immunobloting and qrt-pcr assay, we found that the combination treatment of genistein and gemcitabine resulted in stronger growth inhibition and apoptosis induction through the downregulation of nf-κb activity and akt activation in osteosarcoma cells. |
2012-09-17 |
2023-08-12 |
human |
| Ingrid K Hals, Hirotaka Ogata, Elin Pettersen, Zuheng Ma, Anneli Björklund, Frank Skorpen, Kjartan Wollo Egeberg, Valdemar Gril. Marked over expression of uncoupling protein-2 in beta cells exerts minor effects on mitochondrial metabolism. Biochemical and biophysical research communications. vol 423. issue 2. 2012-09-17. PMID:22634308. |
fourfold induced cells displayed normal viability (mtt, apoptosis), normal cellular insulin contents and, glucose-induced insulin secretion (+27 ± 11%) as well as d-[u-(14)c]-glucose oxidation (+5 ± 9% at 11 mm glucose). |
2012-09-17 |
2023-08-12 |
Not clear |
| Lanhai Lü, Lihong Zhang, Maria Sen Mun Wai, David Tai Wai Yew, Jie X. Exocytosis of MTT formazan could exacerbate cell injury. Toxicology in vitro : an international journal published in association with BIBRA. vol 26. issue 4. 2012-09-07. PMID:22401948. |
our results also indicated that mtt could activate apoptosis related factors such as caspase-8, caspase-3 or accelerate the leakage of cell contents after the appearance of mtt formazan crystals. |
2012-09-07 |
2023-08-12 |
Not clear |
| Xin-Li Li, Jiu-Hong Xu, Ji-Hua Nie, Sai-Jun Fa. Exogenous expression of UHRF1 promotes proliferation and metastasis of breast cancer cells. Oncology reports. vol 28. issue 1. 2012-09-04. PMID:22552622. |
cell proliferation was examined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (mtt) assay; cell cycle distribution and apoptosis were evaluated using flow cytometry; protein expression was determined by western blotting; angiogenesis of xenografts was assessed by microvessel density (mvd); cell invasion was measured using transwell chamber; cell migration was determined by wound scratching assay. |
2012-09-04 |
2023-08-12 |
Not clear |
| Bo Ma, Hanchao Zhang, Jinhuan Wang, Biao Zhang, Xinnv Xu, Binglu Chen. HIV-1 viral protein R (Vpr) induction of apoptosis and cell cycle arrest in multidrug-resistant colorectal cancer cells. Oncology reports. vol 28. issue 1. 2012-09-04. PMID:22552851. |
in this study, an ad-vpr construct was used to infect the multidrug-resistant human colorectal cancer hct-8/5-fu(mdr) cell line in vitro for cell viability, apoptosis, gene expression and gene activity using the mtt, flow cytometry, immunoblotting and gel shift assays, respectively. |
2012-09-04 |
2023-08-12 |
human |
| Xin Yang, Xiongying Miao, Yu Wen, Jixiong Hu, Weidong Dai, Bangliang Yi. A possible connection between adhesion regulating molecule 1 overexpression and nuclear factor kappa B activity in hepatocarcinogenesis. Oncology reports. vol 28. issue 1. 2012-09-04. PMID:22576803. |
in this study, using laboratory techniques including short hairpin rna (shrna)-mediated knockdown, immunohistochemistry (ihc), both semi-quantitative and real-time rt-pcr, western blotting, mtt assay, transwell assay, flow cytometry and electrophoretic mobility shift assay (emsa), the expression of adrm1, the effects of adrm1 knockdown on nf-κb activity, as well as the biological behavior of hcc cells including proliferation, migration, invasion and apoptosis were investigated in the samples from hcc patients and hcc cell lines. |
2012-09-04 |
2023-08-12 |
Not clear |
| Na Wang, Zhihua Li, Fen Tian, Ying Feng, Jintao Huang, Chaohong Li, Fukang Xi. PKCα inhibited apoptosis by decreasing the activity of JNK in MCF-7/ADR cells. Experimental and toxicologic pathology : official journal of the Gesellschaft fur Toxikologische Pathologie. vol 64. issue 5. 2012-08-31. PMID:21106355. |
mtt, dna ladder and flow cytometry were used to detect cell growth inhibition or apoptosis while western blot was used to detect the activation of proteins. |
2012-08-31 |
2023-08-12 |
Not clear |
| Kai-Sheng Liu, Wei-Chao Ding, Shao-Xiang Wang, Zhong Liu, Guo-Wen Xing, Ying Wang, Yi-Fei Wan. The heat shock protein 90 inhibitor SNX-2112 inhibits B16 melanoma cell growth in vitro and in vivo. Oncology reports. vol 27. issue 6. 2012-08-27. PMID:22447251. |
the 3-(4,5-dimetrylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (mtt) assay and flow cytometric analysis demonstrated that snx-2112 dose-dependently inhibited the growth of b16 cells, and induced g0/g1 cell cycle arrest and apoptosis. |
2012-08-27 |
2023-08-12 |
mouse |
| Ting-Tsz Ou, Chau-Jong Wang, Guang-Uei Hung, Cheng-Hsun Wu, Huei-Jane Le. Aqueous Extract of Shi-Liu-Wei-Liu-Qi-Yin Induces G2/M Phase Arrest and Apoptosis in Human Bladder Carcinoma Cells via Fas and Mitochondrial Pathway. Evidence-based complementary and alternative medicine : eCAM. vol 2011. 2012-08-23. PMID:19383839. |
we evaluated the effects of slwlqy on apoptosis-related experiments inducing in tsgh-8301 cells by (i) 3-(4,5-dimethylthiazol-zyl)-2,5-diphenylterazolium bromide (mtt) for cytotoxicity; (ii) cell-cycle analysis and (iii) western blot analysis of the g2/m-phase and apoptosis regulatory proteins. |
2012-08-23 |
2023-08-12 |
mouse |
| Fang Zeng, Yun Cheng Li, Gang Chen, Yong Kui Zhang, Ye Kai Wang, Shi Quan Zhou, Li Na Ma, Ji Hang Zhou, Yan Yan Huang, Wang Yu Zhu, Xiao Guang Li. Nicotine inhibits cisplatin-induced apoptosis in NCI-H446 cells. Medical oncology (Northwood, London, England). vol 29. issue 1. 2012-08-23. PMID:21267677. |
an mtt assay, annexin v-fitc staining, rt-pcr, and western blot were applied to identify the viability of cells, stages of apoptosis, mrna and signaling proteins expression, respectively. |
2012-08-23 |
2023-08-12 |
Not clear |
| Tzuu-Yuan Huang, Che-Wen Hsu, Weng-Cheng Chang, Miin-Yau Wang, June-Fu Wu, Yi-Chiang Hs. Demethoxycurcumin Retards Cell Growth and Induces Apoptosis in Human Brain Malignant Glioma GBM 8401 Cells. Evidence-based complementary and alternative medicine : eCAM. vol 2012. 2012-08-23. PMID:22454662. |
in the present study, the antiproliferation (using the mtt assay, dmc was found to have cytotoxic activities against gbm 8401 cell with ic(50) values at 22.71 μm) and induced apoptosis effects of dmc have been investigated in human brain malignant glioma gbm 8401 cells. |
2012-08-23 |
2023-08-12 |
human |
| Xiao-Chun Wang, Yi-Hua Qiu, Yu-Ping Pen. Interleukin-6 protects cerebellar granule neurons from NMDA-induced neurotoxicity. Sheng li xue bao : [Acta physiologica Sinica]. vol 59. issue 2. 2012-08-20. PMID:17437036. |
methyl-thiazole-tetrazolium (mtt) assay, terminal deoxynucleotidyl transferase-mediated dutp nick end labeling (tunel) method and confocal laser scanning microscope (clsm) were used to detect neuronal vitality, apoptosis and dynamic changes of intracellular ca(2+) levels in the neurons, respectively. |
2012-08-20 |
2023-08-12 |
rat |
| A Anitha, K P Chennazhi, S V Nair, R Jayakuma. 5-flourouracil loaded N,O-carboxymethyl chitosan nanoparticles as an anticancer nanomedicine for breast cancer. Journal of biomedical nanotechnology. vol 8. issue 1. 2012-08-16. PMID:22515092. |
results of anticancer activity via mtt, apoptosis and caspase 3 assays showed the toxicity of the drug loaded nanoparticles towards breast cancer cells. |
2012-08-16 |
2023-08-12 |
Not clear |
| Zufeng Ding, Shijie Liu, Bo Yang, Yubo Fan, Xiaoyan Den. Effect of oxidized low-density lipoprotein concentration polarization on human smooth muscle cells' proliferation, cycle, apoptosis and oxidized low-density lipoprotein uptake. Journal of the Royal Society, Interface. vol 9. issue 71. 2012-08-15. PMID:22048945. |
to clarify the effect of concentration polarization of oxidative modification of low-density lipoproteins (ox-ldls) on human smooth muscle cells (smcs), the proliferation, ox-ldl uptake and apoptosis with smcs cultured on permeable (the permeable group) or non-permeable membranes (the non-permeable group) were analysed by 3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide (mtt) assay, spectrofluorometry and flow cytometry using a parallel-plate flow chamber technique. |
2012-08-15 |
2023-08-12 |
human |