| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Ge Gao, Xin Wang, Xiaoqun Qin, Xinyu Jiang, Daxiong Xiang, Li Xie, Jianguo Hu, Jiesheng Ga. Effects of trimethoxystilbene on proliferation and apoptosis of pulmonary artery smooth muscle cells. Cell biochemistry and biophysics. vol 64. issue 2. 2013-03-04. PMID:22707295. |
the effects on pasmc proliferation were quantified by mtt, while apoptosis was assessed by flow cytometry (annexin v/propidium iodide assay). |
2013-03-04 |
2023-08-12 |
Not clear |
| Agnes N Yoshimoto, Claudio Bernardazzi, Antonio José V Carneiro, Celeste C S Elia, Cesonia A Martinusso, Grasiella M Ventura, Morgana T L Castelo-Branco, Heitor S P de Souz. Hedgehog pathway signaling regulates human colon carcinoma HT-29 epithelial cell line apoptosis and cytokine secretion. PloS one. vol 7. issue 9. 2013-02-25. PMID:23028941. |
viability, apoptosis and cell proliferation were measured by the mtt assay, annexin-v/7-aad staining and brdu uptake, respectively. |
2013-02-25 |
2023-08-12 |
human |
| Zun-Ling Li, Shu-Hong Shao, Shu-Yang Xie, Zhen Yue, Ying M. [Anti-sense nucleic acid of CyclinD1 induces apoptosis of lung adenocarcinoma cancer cell A549]. Sheng li xue bao : [Acta physiologica Sinica]. vol 63. issue 3. 2013-02-21. PMID:21681345. |
mtt method and flow cytometry technique were used to detect cell proliferation and apoptosis, respectively. |
2013-02-21 |
2023-08-12 |
Not clear |
| Xing Xing, Yizhen Hu, Yang Cao, Zhirong X. The apoptosis of bovine lens epithelial cells induced by proteasome inhibitor MG132. Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban. vol 28. issue 4. 2013-02-11. PMID:18704314. |
to investigate the effect of proteasome inhibitor mg132 on the apoptosis of bovine lens epithelial cells (blecs), the cells were treated with mg132 at different concentrations for 12, 24 and 36 h. the cell viability was analyzed by mtt assay and the effect of mg132 on the apoptosis of blecs was assessed by flow cytometry (fcm). |
2013-02-11 |
2023-08-12 |
cattle |
| Lu Han, Li-Bo Du, Anil Kumar, Hong-Ying Jia, Xing-Jie Liang, Qiu Tian, Guang-Jun Nie, Yang Li. Inhibitory effects of trolox-encapsulated chitosan nanoparticles on tert-butylhydroperoxide induced RAW264.7 apoptosis. Biomaterials. vol 33. issue 33. 2013-02-06. PMID:22922024. |
the effects of trolox-cs nps on raw264.7 cells damaged by tert-butylhydroperoxide (t-bhp) were determined by mtt assay for cell viability, mda assay for membrane lipid peroxidation, jc-1 probe and annexin v-fitc/pi double staining for mitochondria membrane potential (mmp) and raw264.7 apoptosis, respectively. |
2013-02-06 |
2023-08-12 |
Not clear |
| Liu Yang, Mei Liu, Zhikai Gu, Jianguo Chen, Yaohua Yan, Jian L. Overexpression of SASH1 related to the decreased invasion ability of human glioma U251 cells. Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine. vol 33. issue 6. 2013-01-31. PMID:22915266. |
using the tetrazolium-based colorimetric (mtt) assay, flow cytometry analyses, transwell invasion chamber experiments, and other methods, we examined the impact of sash1 on the biological behaviors of u251 cells, including effects on viability, cell cycle, apoptosis, and invasion. |
2013-01-31 |
2023-08-12 |
human |
| Yuanyuan Fang, Zhengtao Li, Xiuxia Wang, Shulan Zhan. CIP2A is overexpressed in human ovarian cancer and regulates cell proliferation and apoptosis. Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine. vol 33. issue 6. 2013-01-31. PMID:22923389. |
mtt, colony formation assay, and flow cytometry were carried out to assess the role of cip2a in proliferation, cell cycle, and apoptosis. |
2013-01-31 |
2023-08-12 |
human |
| Yi Qin, Jing Chen, Li Li, Chun-Jie Liao, Yu-Bing Liang, En-Jian Guan, Yu-Bo Xi. Exogenous morphine inhibits human gastric cancer MGC- 803 cell growth by cell cycle arrest and apoptosis induction. Asian Pacific journal of cancer prevention : APJCP. vol 13. issue 4. 2013-01-30. PMID:22799335. |
the viability of cells was assessed by mtt assay, proliferation by colony formation assay, cell cycle progression and apoptosis by flow cytometry and ultrastructural alteration by transmission electron microscopy. |
2013-01-30 |
2023-08-12 |
human |
| Xiaomei Liu, Aiyuan Wang, Hong Gao, Zhengwei Yuan, Yisheng Jia. Expression and role of the inhibitor of apoptosis protein livin in chemotherapy sensitivity of ovarian carcinoma. International journal of oncology. vol 41. issue 3. 2013-01-23. PMID:22766624. |
cell apoptosis and chemosensitivity were evaluated by mtt assay and flow cytometry, respectively, following downregulation of livin expression, and the cleavage of molecular markers of the mitochondrial apoptotic signaling pathway was investigated by immunoblotting. |
2013-01-23 |
2023-08-12 |
human |
| Jing Wang, Shu-Lei Zhao, Yan Li, Mei Meng, Cheng-Yong Qi. 4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone induces retinoic acid receptor β hypermethylation through DNA methyltransferase 1 accumulation in esophageal squamous epithelial cells. Asian Pacific journal of cancer prevention : APJCP. vol 13. issue 5. 2013-01-22. PMID:22901195. |
3-(4,5-dimethyl-thiazol-2yl)- 2,5-diphenyltetrazolium bromide (mtt) and flow cytometric analysis demonstrated that nnk treatment in het- 1a cells could enhance cell proliferation and inhibit cell apoptosis in a dose-dependent manner. |
2013-01-22 |
2023-08-12 |
Not clear |
| Yue Chen, Hong Zou, Li-Ying Yang, Yuan Li, Li Wang, Yan Hao, Ju-Lun Yan. ER81-shRNA inhibits growth of triple-negative human breast cancer cell line MDA-MB-231 in vivo and in vitro. Asian Pacific journal of cancer prevention : APJCP. vol 13. issue 5. 2013-01-22. PMID:22901226. |
er81-shrna was employed to silence er81 expression in the mda-mb-231 cell line, and mtt, colony-forming assays, and flow cytometry were used to detect cell proliferation, colony-forming capability, cell cycle distribution, and cell apoptosis in vitro. |
2013-01-22 |
2023-08-12 |
mouse |
| Catherine A Sánchez, Alejandro J Mercado, Héctor R Contreras, Juan C Cabezas, Christian C Huidobro, Enrique A Castelló. Pharmacoperone IN3 enhances the apoptotic effect of leuprolide in prostate cancer cells by increasing the gonadotropin-releasing hormone receptor in the cell membrane. Anti-cancer drugs. vol 23. issue 9. 2013-01-21. PMID:22713594. |
cell survival was evaluated by 1-(4,5-dimethylthiazol-2-yl)-3,5-diphenylformazan (mtt) thiazolyl blue formazan and cell cycle and apoptosis by flow cytometry. |
2013-01-21 |
2023-08-12 |
human |
| Zhixiong Chen, Jiong Yan. Growth suppression of human lung cancer cells and implanted tumors by adenovirus-mediated transfer of the PTEN gene. Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban. vol 30. issue 2. 2013-01-18. PMID:20407863. |
the proliferation and the apoptosis rates of the a549 cells with ad-pten-egfp transfection or not was detected by mtt and flow cytometry. |
2013-01-18 |
2023-08-12 |
mouse |
| Xianfeng Fang, Xuxin Zhang, Meng Zhou, Jiawen L. Effects of progesterone on the growth regulation in classical progesterone receptor-negative malignant melanoma cells. Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban. vol 30. issue 2. 2013-01-18. PMID:20407880. |
for signal transduction pathway studies, the cells were pretreated with prog receptor antagonist (ru486, 1 x 10(-7) mol/l) or mapk inhibitor (u0126, 5 x 10(-6) mol/l) for 1 h and then co-incubated with prog (10(-9) mol/l) for another 24 h. indirect immunofluorescence assay, mtt, flow cytometry and western blotting were used for assessing the npr expression, cell growth, cell apoptosis and erk1/2 phosphorylation, respectively. |
2013-01-18 |
2023-08-12 |
Not clear |
| L Cipa. Novel nonclassical antifolate, 2-[N-(2´-Hydroxyethyl)amino]methyl-3H-quinazolin-4-one, with a potent antineoplastic activity toward leukemia cells. Neoplasma. vol 59. issue 6. 2013-01-18. PMID:22862178. |
the antiproliferative activity of heamq was determined by mtt assay and its effects on cell cycle progression and apoptosis were studied by flow cytometry, and by immunoblots, respectively. |
2013-01-18 |
2023-08-12 |
mouse |
| Christiane Pavani, Yassuko Iamamoto, Maurício S Baptist. Mechanism and efficiency of cell death of type II photosensitizers: effect of zinc chelation. Photochemistry and photobiology. vol 88. issue 4. 2013-01-17. PMID:22283143. |
zinc-chelated porphyrins, which are poorly accumulated in mitochondria, show higher efficiency of cell death with features of apoptosis (higher mtt response compared with trypan blue staining, specific acridine orange/ethidium bromide staining, loss of mitochondrial transmembrane potential, stronger cytochrome c release and larger sub-g1 cell population), whereas nonchelated porphyrins, which are considerably more concentrated in mitochondria, triggered mainly necrotic cell death. |
2013-01-17 |
2023-08-12 |
Not clear |
| Viviana Vergaro, Yuri M Lvov, Stefano Leporatt. Halloysite clay nanotubes for resveratrol delivery to cancer cells. Macromolecular bioscience. vol 12. issue 9. 2013-01-14. PMID:22887783. |
mtt measurements performed with a neoplastic cell lines model system (mcf-7) as function of the resveratrol-loaded nanotubes concentration and incubation time indicate that drug-loaded halloysite strongly increase of cytotoxicity leading to cell apoptosis. |
2013-01-14 |
2023-08-12 |
Not clear |
| Chongmu Lee, Chanseok Hong, Jungkeun Lee, Mikwon Son, Soon-Sun Hon. Comparison of oxidized porous silicon with bare porous silicon as a photothermal agent for cancer cell destruction based on in vitro cell test results. Lasers in medical science. vol 27. issue 5. 2013-01-10. PMID:22160211. |
the temperature measurement and in vitro cell tests including the annexin v-fluorescein isothiocyanate (fitc) apoptosis assay tests, mtt assay tests, and trypan blue cell death assay tests were performed to compare the photothermal properties and the cytotoxic effect of psio with those of psi in combination with an 808-nm nir laser. |
2013-01-10 |
2023-08-12 |
Not clear |
| Áurea F Castilho, Célia A Aveleira, Ermelindo C Leal, Núria F Simões, Carolina R Fernandes, Rita I Meirinhos, Filipa I Baptista, António F Ambrósi. Heme oxygenase-1 protects retinal endothelial cells against high glucose- and oxidative/nitrosative stress-induced toxicity. PloS one. vol 7. issue 8. 2013-01-10. PMID:22879979. |
cell viability and apoptosis were assessed by mtt assay, hoechst staining, tunel assay and annexin v labeling. |
2013-01-10 |
2023-08-12 |
Not clear |
| Ling Zhang, Tao He, Hong Cui, Yunjian Wang, Changshan Huang, Feng Ha. Effects of AFP gene silencing on apoptosis and proliferation of a hepatocellular carcinoma cell line. Discovery medicine. vol 14. issue 75. 2013-01-10. PMID:22935208. |
furthermore, mtt (methyl thiazolyl tetrazelium) assay showed that cells transfected with sirna-afp proliferated at a significantly lower speed than the other two groups and flat plate clone formation assay also witnessed less clones with diameters of more than 75 μm in sirna-afp immunofluorescence indicating that the apoptosis rate of cells transfected with sirna-afp was significantly higher than the other two groups. |
2013-01-10 |
2023-08-12 |
Not clear |