| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Jing Wang, Jing Cai, Zhimin Li, Sha Hu, Lili Yu, Lan Xiao, Zehua Wan. Expression and biological function of N-myc down-regulated gene 1 in human cervical cancer. Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban. vol 30. issue 6. 2013-04-12. PMID:21181370. |
its effects on cell proliferation, migration, invasion, cell cycle and apoptosis were detected by mtt, transwell migration assay and flow cytometry (fcm), respectively. |
2013-04-12 |
2023-08-12 |
human |
| H Jiang, L Zhang, J Liu, Z Chen, R Na, G Ding, H Zhang, Q Din. Knockdown of zinc finger protein X-linked inhibits prostate cancer cell proliferation and induces apoptosis by activating caspase-3 and caspase-9. Cancer gene therapy. vol 19. issue 10. 2013-04-09. PMID:22898899. |
the effects of sirna targeting zfx on growth, cell cycle and apoptosis of pc-3 cells were evaluated by mtt assay and flow cytometry. |
2013-04-09 |
2023-08-12 |
Not clear |
| Wen-Yu Qian, Dong-Mei Sun, Rong-Rong Zhu, Xi-Ling Du, Hui Liu, Shi-Long Wan. pH-sensitive strontium carbonate nanoparticles as new anticancer vehicles for controlled etoposide release. International journal of nanomedicine. vol 7. 2013-04-08. PMID:23185118. |
meanwhile, it was also found that the blank scns were almost nontoxic to normal cells, and escn systems were evidently more potent in antitumor activity compared with free etoposide, as confirmed by a cytotoxicity test using an mtt assay and an apoptosis test with fluorescence-activated cell sorter (facs) analysis. |
2013-04-08 |
2023-08-12 |
Not clear |
| Hai-Lu Wu, Xin Gao, Zong-Dan Jiang, Zhao-Tao Duan, Shu-Kui Wang, Bang-Shun He, Zhen-Yu Zhang, Hong-Guang Xi. Attenuated expression of the tight junction proteins is involved in clopidogrel-induced gastric injury through p38 MAPK activation. Toxicology. vol 304. 2013-04-08. PMID:23220562. |
after human gastric epithelial cell line ges-1 had been treated with clopidogrel (0.5-2.5 mm), the cell proliferation was examined by mtt assay, apoptosis was measured with dapi staining and flow cytometry analysis, and the barrier function of the tight junctions (tj) was evaluated by permeability measurement and transmission electron microscopy. |
2013-04-08 |
2023-08-12 |
human |
| Bo Chen, Xiaoli Ma, Shifeng Liu, Weihong Zhao, Jianqing W. Inhibition of lung cancer cells growth, motility and induction of apoptosis by Klotho, a novel secreted Wnt antagonist, in a dose-dependent manner. Cancer biology & therapy. vol 13. issue 12. 2013-04-05. PMID:22922788. |
effects of klotho on cell growth and motility were assessed using mtt and scratch motility assay, and the apoptosis was assessed by tunel. |
2013-04-05 |
2023-08-12 |
mouse |
| Yan Li, Yi Jiang, Yicong Wan, Lin Zhang, Weiwei Tang, Jingjing Ma, Shan Wu, Wenjun Chen. Medroxyprogestogen enhances apoptosis of SKOV-3 cells via inhibition of the PI3K/Akt signaling pathway. Journal of biomedical research. vol 27. issue 1. 2013-04-05. PMID:23554793. |
medroxyprogestogen-induced apoptosis was assessed by mtt assays and annexin v apoptosis assay. |
2013-04-05 |
2023-08-12 |
Not clear |
| Chao Zhang, Yong Liang Chi, Ping Yu Wang, Ya Qi Wang, Yan Xia Zhang, Jingti Deng, Chang Jun Lv, Shu Yang Xi. miR-511 and miR-1297 inhibit human lung adenocarcinoma cell proliferation by targeting oncogene TRIB2. PloS one. vol 7. issue 10. 2013-04-04. PMID:23071539. |
the mtt test showed inhibition of a549 cell proliferation and annexin v-fitc/pi dual staining showed increased apoptosis in the mir-511- and mir-1297-treated cells compared to the nc cultures. |
2013-04-04 |
2023-08-12 |
human |
| Liang Zhou, Ning Zhang, Qing-Jun Li, Wei Sun, Yong Zhang, De-Sheng Wang, Ke-Feng Do. Associations between high levels of Notch1 expression and high invasion and poor overall survival in hepatocellular carcinoma. Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine. vol 34. issue 1. 2013-03-28. PMID:23179396. |
the activity of mmp-2/mmp-9 was quantified by enzyme-linked immunosorbent assay, and cellular apoptosis was analyzed using the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2h-tetrazolium bromide (mtt) assay. |
2013-03-28 |
2023-08-12 |
Not clear |
| Kinga Majchrzak, Karol M Pawłowski, Emilia J Orzechowska, Izabella Dolka, Joanna Mucha, Tomasz Motyl, Magdalena Kró. A role of ghrelin in canine mammary carcinoma cells proliferation, apoptosis and migration. BMC veterinary research. vol 8. 2013-03-25. PMID:22999388. |
for apoptosis analysis the annexin v and propidium iodide dual staining was applied whereas cell proliferation was evaluated by mtt assay and brdu incorporation test. |
2013-03-25 |
2023-08-12 |
human |
| Zhong-Yan Liu, Hai-Ou Qiu, Xiao-Jian Yuan, Yan-Yan Ni, Jian-Jun Sun, Wei Jing, Yue-Zu Fa. Suppression of lymphangiogenesis in human lymphatic endothelial cells by simultaneously blocking VEGF-C and VEGF-D/VEGFR-3 with norcantharidin. International journal of oncology. vol 41. issue 5. 2013-03-22. PMID:22922710. |
here, we investigated the effect of nctd on proliferation, apoptosis, migration, invasion and the lymphatic tube formation, lymphangiogenesis, of human lymphatic endothelial cells (hlecs) in vitro by mtt, proliferation assay, hoechst staining and flow cytometry, scraping line method, matrigel invasion assay, inverted or fluorescence microscope and transmission electron microscope. |
2013-03-22 |
2023-08-12 |
human |
| Bo Li, Yuehua Yang, Shengdan Jiang, Binbin Ni, Ke Chen, Leisheng Jian. Adenovirus-mediated overexpression of BMP-9 inhibits human osteosarcoma cell growth and migration through downregulation of the PI3K/AKT pathway. International journal of oncology. vol 41. issue 5. 2013-03-22. PMID:22948234. |
as a result, osteosarcoma cell proliferation and migration were significantly diminished by adbmp-9, indicated by mtt and wound-healing assays, and cell apoptosis was markedly induced, indicated by hoechst 33342/pi assay and annexin v-fitc apoptosis detection. |
2013-03-22 |
2023-08-12 |
mouse |
| Yang-guang Han, Jing Xu, Zhi-gui Li, Guo-gang Ren, Zhuo Yan. In vitro toxicity of multi-walled carbon nanotubes in C6 rat glioma cells. Neurotoxicology. vol 33. issue 5. 2013-03-21. PMID:22728153. |
to elucidate the possible mechanisms of cytotoxicity induced by mwcnt, mtt assay and flow cytometry analysis for apoptosis and cell cycle, mda and sod assays for oxidative stress were quantitatively assessed. |
2013-03-21 |
2023-08-12 |
rat |
| Ling Xu, Xiu-Juan Qu, Yun-Peng Liu, Ying-Ying Xu, Jing Liu, Ke-Zuo Hou, Ye Zhan. Protective autophagy antagonizes oxaliplatin-induced apoptosis in gastric cancer cells. Chinese journal of cancer. vol 30. issue 7. 2013-03-20. PMID:21718595. |
cell proliferation was measured using mtt assay, and apoptosis was determined by flow cytometry. |
2013-03-20 |
2023-08-12 |
Not clear |
| Xian Chen, Yi Wang, Hongmei Luo, Zhigang Luo, Tao Zhang, Ning Yang, Xiangyang Long, Huang Xie, Weibing Qiu, Biao Zhang, Jun Ding, Luoyan Yan. β-elemene acts as an antitumor factor and downregulates the expression of survivin, Bcl-xL and Mta-1. Molecular medicine reports. vol 6. issue 5. 2013-03-19. PMID:22895653. |
the survival rate and apoptosis of t24 cells following β-elemene treatment was detected by mtt assay and tunel staining. |
2013-03-19 |
2023-08-12 |
Not clear |
| Jian Ma, Hai-min Chen, Xiao-jun Yan, Feng Wang, Wei-feng X. [Astaxanthin inhibits sodium azide-induced cytotoxicity in hepatocyte L-02 cells probably by H+ transferring function]. Yao xue xue bao = Acta pharmaceutica Sinica. vol 46. issue 5. 2013-03-18. PMID:21800538. |
hepatocyte l-02 cells were exposed to 100 mmol.l-1 nan3 with various concentrations of astaxanthin pre-incubated, then the cell viability was measured by mtt method; the level of reactive oxygen species (ros) was determined by dcfh-da method; the changes of mitochondrial membrane potential (mmp) and apoptosis ratio were detected by jc-1 method and annexin v-fitc/pi double stain method, respectively. |
2013-03-18 |
2023-08-12 |
Not clear |
| Gonghua Hu, Haiyan Huang, Lingqing Yang, Caigao Zhong, Bo Xia, Yuan Yang, Jianjun Liu, Desheng Wu, Qingchen Liu, Zhixiong Zhuan. Down-regulation of Polη expression leads to increased DNA damage, apoptosis and enhanced S phase arrest in L-02 cells exposed to hydroquinone. Toxicology letters. vol 214. issue 2. 2013-03-18. PMID:22981619. |
in this study, cell survival rate was determined using the mtt assay, dna damage was determined by the comet assay, apoptosis and cell cycle distribution were determined using flow cytometry, the mrna expression levels of polη were determined by real-time pcr, and the protein expression levels of polη and γ-h2ax were determined by western blot, γ-h2ax foci were visualized by confocal laser scanning fluorescence microscopy after cells were exposed to hq at various concentrations for 24h in vitro. |
2013-03-18 |
2023-08-12 |
Not clear |
| Xiaochun Sun, Yan Xie, Lele Wu, Wei Zhu, Jiabo Hu, Rongzhu Lu, Wenrong X. Lead acetate reduces the ability of human umbilical cord mesenchymal stem cells to support hematopoiesis in vitro. Molecular medicine reports. vol 6. issue 4. 2013-03-14. PMID:22858795. |
in the present study, cell count, mtt assay, apoptosis assay, osteogenic differentiation, cell histochemical staining, cell cycle analysis, colony forming assay and rt-pcr were used. |
2013-03-14 |
2023-08-12 |
human |
| Yu-Wei Liang, Chi-Chang Chang, Chao-Ming Hung, Tzu-Yu Chen, Tzuu-Yuan Huang, Yi-Chiang Hs. Preclinical Activity of Simvastatin Induces Cell Cycle Arrest in G1 via Blockade of Cyclin D-Cdk4 Expression in Non-Small Cell Lung Cancer (NSCLC). International journal of molecular sciences. vol 14. issue 3. 2013-03-14. PMID:23481641. |
in this study, an mtt assay revealed the cytotoxic activity of sim against human large cell lung cancer (non-small cell lung cancer; nsclc) cells (nci-h460); however, induced apoptosis was not observed in nci-h460 cells. |
2013-03-14 |
2023-08-12 |
human |
| Masaki Kobayashi, Kazuhiro Hidaka, Ikumi Chisaki, Natsuko Takahashi, Jiro Ogura, Shirou Itagaki, Takeshi Hirano, Hiroaki Yamaguchi, Ken Isek. [Effects of acidification and alkalinization agents on statins-induced muscle toxicity]. Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan. vol 132. issue 5. 2013-03-12. PMID:22687697. |
statin-induced reduction of cell viability and apoptosis was measured by the 3-(4,5-dimethylthiazol-2-thiazolyl)-2,5-diphenyl tetrazolium bromide (mtt) assay and caspase assay. |
2013-03-12 |
2023-08-12 |
human |
| Wan Yong Ho, Swee Keong Yeap, Chai Ling Ho, Raha Abdul Rahim, Noorjahan Banu Alithee. Development of multicellular tumor spheroid (MCTS) culture from breast cancer cell and a high throughput screening method using the MTT assay. PloS one. vol 7. issue 9. 2013-03-07. PMID:22970274. |
the mtt assay emerged as a better indicator to apoptosis event in comparison to the ldh release assay. |
2013-03-07 |
2023-08-12 |
Not clear |