| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Weiguo Chen, Jianbang Wang, Xihui Wang, Pan Chang, Meng Lian. Knockdown of hypoxia-inducible factor 1-alpha (HIF1α) interferes with angiopoietin-like protein 2 (ANGPTL2) to attenuate high glucose-triggered hypoxia/reoxygenation injury in cardiomyocytes. Bioengineered. vol 13. issue 1. 2022-01-03. PMID:34974813. |
cell viability and apoptosis were, respectively, estimated by cell counting kit 8 (cck-8) and tunel assays. |
2022-01-03 |
2023-08-13 |
Not clear |
| Adi Knigin, Shani Avniel-Polak, Gil Leibowitz, Kira Oleinikov, David J Gross, Simona Grozinsky-Glasber. The Autophagy Inhibitor Chloroquine, Alone or in Combination with mTOR Inhibitors, Displays Anti-Tumor Effects in In Vitro and In Vivo Lung Carcinoid Models. Cancers. vol 13. issue 24. 2021-12-24. PMID:34944946. |
cytotoxicity was measured using a cytotoxicity kit; apoptosis was measured by pi facs staining and western blotting. |
2021-12-24 |
2023-08-13 |
Not clear |
| A Khodaei, F Jahanmard, H R Madaah Hosseini, R Bagheri, A Dabbagh, H Weinans, S Amin Yavar. Controlled temperature-mediated curcumin release from magneto-thermal nanocarriers to kill bone tumors. Bioactive materials. vol 11. 2021-12-23. PMID:34938916. |
the bone tumor killing efficacy of the released curcumin from the carrier in combination with the hyperthermia was studied on mg-63 osteosarcoma cells through alamar blue assay, live-dead staining and apoptosis caspase 3/7 activation kit. |
2021-12-23 |
2023-08-13 |
Not clear |
| Ping Liu, Jin Huang, Wanzhen Mei, Xingfang Zeng, Cheng Wang, Chuan Wen, Jing X. Epigallocatechin-3-gallate protects cardiomyocytes from hypoxia-reoxygenation damage via raising autophagy related 4C expression. Bioengineered. vol 12. issue 2. 2021-12-02. PMID:34699312. |
cell viability, apoptosis, reactive oxygen species (ros) production, atp level were tested via cck-8 assay, annexin v-fitc/pi staining, dcfh-da staining and atp assay kit, respectively. |
2021-12-02 |
2023-08-13 |
Not clear |
| Danfeng Wang, Wei Zhao, Jianrong Liu, Yimin Wang, Caixia Yuan, Fang Zhang, Guorong Jin, Qin Qi. Effects of HIF-1α on Spermatogenesis of Varicocele Rats by Regulating VEGF/PI3K/Akt Signaling Pathway. Reproductive sciences (Thousand Oaks, Calif.). vol 28. issue 4. 2021-11-30. PMID:33237516. |
apoptosis of spermatogenic cells in rat testis was tested by tunel kit. |
2021-11-30 |
2023-08-13 |
rat |
| Feixiang Chen, Jiadong Zhu, Wei Wan. Ulinastatin Attenuates LPS-Induced Inflammation and Inhibits Endoplasmic Reticulum Stress-Induced Apoptosis in Renal Tubular Epithelial Cells via Regulation of the TLR4/NF-κB and Nrf2/HO-1 Pathways. Inflammation. vol 44. issue 6. 2021-11-26. PMID:34585338. |
this study was intended to explore the underlying mechanism by which ulinastatin (uti) influenced the inflammation and apoptosis of renal tubular epithelial cells, hk-2.the effects of uti on the cell viability of hk-2 cells were first measured by mtt and lactate dehydrogenase (ldh) detection kit. |
2021-11-26 |
2023-08-13 |
Not clear |
| César Serrano, Suzanne Georg. Gastrointestinal Stromal Tumor: Challenges and Opportunities for a New Decade. Clinical cancer research : an official journal of the American Association for Cancer Research. vol 26. issue 19. 2021-11-25. PMID:32601076. |
to succeed, we will need to better understand the mechanisms behind adaptation to kit inhibition and apoptosis evasion, tumor evolution after successive lines of treatment, and to explore clinically novel creative therapeutic strategies, with the overarching goal to tackle the intrinsic oncogenic complexity while minimizing adverse events. |
2021-11-25 |
2023-08-13 |
Not clear |
| Xianxuan Wang, Hongshu Sui, Yanping Su, Shang Zha. Protective effects of sodium selenite on insulin secretion and diabetic retinopathy in rats with type 1 diabetes mellitus. Pakistan journal of pharmaceutical sciences. vol 34. issue 5. 2021-11-22. PMID:34803009. |
apoptosis level of pancreatic cells in rats was determined by apoptosis kit. |
2021-11-22 |
2023-08-13 |
rat |
| Qing Wang, Lujing Ying, Bing Wei, Yikui Ji, Yi X. Effects of quercetin on apoptosis and extracellular matrix degradation of chondrocytes induced by oxidative stress-mediated pyroptosis. Journal of biochemical and molecular toxicology. 2021-11-18. PMID:34791735. |
cell counting kit-8 (cck-8 kit), flow cytometry, enzyme-linked immunosorbent assay (elisa) kit, western blot, dichlorodihydrofluorescein diacetate (dcfh-da) kit, thiobarbituric acid (tba) test, toluidine blue staining, hematoxylin eosin (he) staining and terminal deoxynucleotidyl transferase (tdt)-mediated dutp nick-end labeling (tunel) staining were used to evaluate cell viability, cell apoptosis, inflammatory cytokines level, protein expression, reactive oxygen species (ros) level, malondialdehyde (mda) content, morphological changes, and chondrocyte apoptosis of cartilage, respectively. |
2021-11-18 |
2023-08-13 |
rat |
| Min Li, Yufeng Xu. The upregulation of Nur77 decreases ketamine-induced hippocampal neurons toxicity in rats. Neuroreport. vol 32. issue 17. 2021-11-16. PMID:34718249. |
neurons apoptosis and reactive oxygen species (ros) were determined by a related kit using flow cytometry. |
2021-11-16 |
2023-08-13 |
rat |
| Qi Zhang, Tao Xue, Jianming Guan, Wei Wang, Ji Shi, Jianzhong Lu, Xiping Jian. Irigenin alleviates angiotensin II-induced oxidative stress and apoptosis in HUVEC cells by activating Nrf2 pathway. Drug development research. vol 82. issue 7. 2021-11-15. PMID:33634899. |
after human umbilical vein endothelial cell lines (huvecs) were treated with ang ii (100, 200, 300 and 400 nmol/l) alone, ir (2.5, 5, 10, 20 and 40 μmol/l) alone or ang ii plus ir for 24 h, huvecs viability, lactate dehydrogenase (ldh), apoptosis, oxidative stress, apoptosis-related protein and nuclear factor e2-related factor 2 (nrf2) levels were detected by cell counting kit (cck)-8 assay, enzyme-linked immunosorbent assay, flow cytometry and western blot. |
2021-11-15 |
2023-08-13 |
human |
| Hong-Yan Du, Rong Wang, Jian-Liang Li, Huang Luo, Xiao-Yan Xie, Ran Yan, Yue-Ling Jian, Jin-Ying Ca. Ligustrazine induces viability, suppresses apoptosis and autophagy of retinal ganglion cells with ischemia/reperfusion injury through the PI3K/Akt/mTOR signaling pathway. Bioengineered. vol 12. issue 1. 2021-11-12. PMID:33522374. |
cell viability, autophagy, and apoptosis were evaluated by cell counting kit 8 assay, transmission electron microscopy, and tunel staining after treatment with ligustrazine, pi3k inhibitor ly294002, and/or mtor inhibitor rapamycin, respectively. |
2021-11-12 |
2023-08-13 |
Not clear |
| Tongyin Zhang, Daofei Ji, Junyi Sun, Jiangling Song, Limin Nie, Na Su. NPAS4 suppresses propofol-induced neurotoxicity by inhibiting autophagy in hippocampal neuronal cells. Archives of biochemistry and biophysics. vol 711. 2021-11-12. PMID:34418347. |
cell viability, lactate dehydrogenase (ldh) release, caspase-3 activity, and apoptosis were evaluated by mtt, a ldh-cytotoxicity assay kit, a caspase-3 colorimetric assay kit, and tunel assay, respectively. |
2021-11-12 |
2023-08-13 |
Not clear |
| Yuxing Huang, Lisha Deng, Lin Zeng, Shanlin Bao, Kun Ye, Chengxun Li, Xiaolin Hou, Yuan Yao, Dingjun Li, Zhen Xion. Silencing of H19 alleviates oxygen-glucose deprivation/reoxygenation-triggered injury through the regulation of the miR-1306-5p/BCL2L13 axis. Metabolic brain disease. vol 36. issue 8. 2021-11-11. PMID:34436746. |
cell viability and apoptosis were determined by the cell counting-8 kit (cck-8) assay and flow cytometry, respectively. |
2021-11-11 |
2023-08-13 |
rat |
| Junping Guo, Wei Chen, Beibei Bao, Dayong Zhang, Jianping Pan, Mao Zhan. Protective effect of berberine against LPS-induced endothelial cell injury via the JNK signaling pathway and autophagic mechanisms. Bioengineered. vol 12. issue 1. 2021-11-10. PMID:33896366. |
cell viability, proliferation, and apoptosis were detected by the cck-8 assay, the edu kit, and flow cytometry, respectively, and autophagy-related protein expression, the number of autophagic vacuoles, and lc3 double-fluorescence were examined using western blot analysis, transmission electron microscopy, and confocal microscopy, respectively. |
2021-11-10 |
2023-08-13 |
human |
| Leilei Yang, Tienan Bi, Shenkang Zhou, Yuefu Lan, Ruili Zhan. CircRASSF2 facilitates the proliferation and metastasis of colorectal cancer by mediating the activity of Wnt/β-catenin signaling pathway by regulating the miR-195-5p/FZD4 axis. Anti-cancer drugs. vol 32. issue 9. 2021-11-09. PMID:33929991. |
cell counting kit 8 assay, colony formation assay, flow cytometry and transwell assay were used to determine the proliferation, apoptosis, migration and invasion of cells, respectively. |
2021-11-09 |
2023-08-13 |
Not clear |
| Wei Tang, Zong-Duo Guo, Wei-Na Chai, Dong-Lin Du, Xiao-Min Yang, Lang Cao, Hong Chen, Chao Zhou, Chong-Jie Cheng, Xiao-Chuan Sun, Zhi-Jian Huang, Jian-Jun Zhon. Downregulation of miR-491-5p promotes neovascularization after traumatic brain injury. Neural regeneration research. vol 17. issue 3. 2021-11-02. PMID:34380897. |
cell counting kit 8 (cck-8) assay, flow cytometry, and 2?,7?-dichlorofluorescein diacetate (dcfh-da) assay results confirmed that the downregulation of mir-491-5p increased brain microvascular endothelial cell viability, reduced cell apoptosis, and alleviated oxidative stress under oxygen-glucose deprivation conditions. |
2021-11-02 |
2023-08-13 |
mouse |
| Ze Li, Junyi Jiang, Xiaohua Yi, Guoyan Wang, Shuhui Wang, Xiuzhu Su. miR-18b regulates the function of rabbit ovary granulosa cells. Reproduction, fertility, and development. vol 33. issue 5. 2021-10-29. PMID:33641714. |
furthermore, the effects of mir-18b on the proliferation, cell cycle and apoptosis of gcs were investigated using a cell counting kit (cck-8) proliferation assay, detection of annexin v-fluorescein isothiocyanate apoptosis, flow cytometry and quantitative polymerase chain reaction. |
2021-10-29 |
2023-08-13 |
rabbit |
| Zhifeng Shi, Zhixiong Zheng, Xiaodan Lin, Hengzhang M. Long Noncoding RNA MALAT1 Regulates the Progression of Atherosclerosis by miR-330-5p/NF-κB Signal Pathway. Journal of cardiovascular pharmacology. vol 78. issue 2. 2021-10-29. PMID:34554676. |
flow cytometry assay, cell counting kit 8 assay, triglyceride, and total cholesterol detection assays were used to detect the apoptosis, viability, and lipid indexes of thp-1 macrophages-derived foam cells. |
2021-10-29 |
2023-08-13 |
mouse |
| Pan Ji, Xuyang Song, Zhiping L. Knockdown of circ_0004104 Alleviates Oxidized Low-Density Lipoprotein-Induced Vascular Endothelial Cell Injury by Regulating miR-100/TNFAIP8 Axis. Journal of cardiovascular pharmacology. vol 78. issue 2. 2021-10-29. PMID:34554678. |
vec apoptosis rate was assessed using flow cytometry, and caspase-3 activity was measured using a caspase-3 assay kit. |
2021-10-29 |
2023-08-13 |
Not clear |