| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Qing Wang, Lujing Ying, Bing Wei, Yikui Ji, Yi X. Effects of quercetin on apoptosis and extracellular matrix degradation of chondrocytes induced by oxidative stress-mediated pyroptosis. Journal of biochemical and molecular toxicology. 2021-11-18. PMID:34791735. |
cell counting kit-8 (cck-8 kit), flow cytometry, enzyme-linked immunosorbent assay (elisa) kit, western blot, dichlorodihydrofluorescein diacetate (dcfh-da) kit, thiobarbituric acid (tba) test, toluidine blue staining, hematoxylin eosin (he) staining and terminal deoxynucleotidyl transferase (tdt)-mediated dutp nick-end labeling (tunel) staining were used to evaluate cell viability, cell apoptosis, inflammatory cytokines level, protein expression, reactive oxygen species (ros) level, malondialdehyde (mda) content, morphological changes, and chondrocyte apoptosis of cartilage, respectively. |
2021-11-18 |
2023-08-13 |
rat |
| Min Li, Yufeng Xu. The upregulation of Nur77 decreases ketamine-induced hippocampal neurons toxicity in rats. Neuroreport. vol 32. issue 17. 2021-11-16. PMID:34718249. |
neurons apoptosis and reactive oxygen species (ros) were determined by a related kit using flow cytometry. |
2021-11-16 |
2023-08-13 |
rat |
| Qi Zhang, Tao Xue, Jianming Guan, Wei Wang, Ji Shi, Jianzhong Lu, Xiping Jian. Irigenin alleviates angiotensin II-induced oxidative stress and apoptosis in HUVEC cells by activating Nrf2 pathway. Drug development research. vol 82. issue 7. 2021-11-15. PMID:33634899. |
after human umbilical vein endothelial cell lines (huvecs) were treated with ang ii (100, 200, 300 and 400 nmol/l) alone, ir (2.5, 5, 10, 20 and 40 μmol/l) alone or ang ii plus ir for 24 h, huvecs viability, lactate dehydrogenase (ldh), apoptosis, oxidative stress, apoptosis-related protein and nuclear factor e2-related factor 2 (nrf2) levels were detected by cell counting kit (cck)-8 assay, enzyme-linked immunosorbent assay, flow cytometry and western blot. |
2021-11-15 |
2023-08-13 |
human |
| Hong-Yan Du, Rong Wang, Jian-Liang Li, Huang Luo, Xiao-Yan Xie, Ran Yan, Yue-Ling Jian, Jin-Ying Ca. Ligustrazine induces viability, suppresses apoptosis and autophagy of retinal ganglion cells with ischemia/reperfusion injury through the PI3K/Akt/mTOR signaling pathway. Bioengineered. vol 12. issue 1. 2021-11-12. PMID:33522374. |
cell viability, autophagy, and apoptosis were evaluated by cell counting kit 8 assay, transmission electron microscopy, and tunel staining after treatment with ligustrazine, pi3k inhibitor ly294002, and/or mtor inhibitor rapamycin, respectively. |
2021-11-12 |
2023-08-13 |
Not clear |
| Tongyin Zhang, Daofei Ji, Junyi Sun, Jiangling Song, Limin Nie, Na Su. NPAS4 suppresses propofol-induced neurotoxicity by inhibiting autophagy in hippocampal neuronal cells. Archives of biochemistry and biophysics. vol 711. 2021-11-12. PMID:34418347. |
cell viability, lactate dehydrogenase (ldh) release, caspase-3 activity, and apoptosis were evaluated by mtt, a ldh-cytotoxicity assay kit, a caspase-3 colorimetric assay kit, and tunel assay, respectively. |
2021-11-12 |
2023-08-13 |
Not clear |
| Yuxing Huang, Lisha Deng, Lin Zeng, Shanlin Bao, Kun Ye, Chengxun Li, Xiaolin Hou, Yuan Yao, Dingjun Li, Zhen Xion. Silencing of H19 alleviates oxygen-glucose deprivation/reoxygenation-triggered injury through the regulation of the miR-1306-5p/BCL2L13 axis. Metabolic brain disease. vol 36. issue 8. 2021-11-11. PMID:34436746. |
cell viability and apoptosis were determined by the cell counting-8 kit (cck-8) assay and flow cytometry, respectively. |
2021-11-11 |
2023-08-13 |
rat |
| Junping Guo, Wei Chen, Beibei Bao, Dayong Zhang, Jianping Pan, Mao Zhan. Protective effect of berberine against LPS-induced endothelial cell injury via the JNK signaling pathway and autophagic mechanisms. Bioengineered. vol 12. issue 1. 2021-11-10. PMID:33896366. |
cell viability, proliferation, and apoptosis were detected by the cck-8 assay, the edu kit, and flow cytometry, respectively, and autophagy-related protein expression, the number of autophagic vacuoles, and lc3 double-fluorescence were examined using western blot analysis, transmission electron microscopy, and confocal microscopy, respectively. |
2021-11-10 |
2023-08-13 |
human |
| Leilei Yang, Tienan Bi, Shenkang Zhou, Yuefu Lan, Ruili Zhan. CircRASSF2 facilitates the proliferation and metastasis of colorectal cancer by mediating the activity of Wnt/β-catenin signaling pathway by regulating the miR-195-5p/FZD4 axis. Anti-cancer drugs. vol 32. issue 9. 2021-11-09. PMID:33929991. |
cell counting kit 8 assay, colony formation assay, flow cytometry and transwell assay were used to determine the proliferation, apoptosis, migration and invasion of cells, respectively. |
2021-11-09 |
2023-08-13 |
Not clear |
| Wei Tang, Zong-Duo Guo, Wei-Na Chai, Dong-Lin Du, Xiao-Min Yang, Lang Cao, Hong Chen, Chao Zhou, Chong-Jie Cheng, Xiao-Chuan Sun, Zhi-Jian Huang, Jian-Jun Zhon. Downregulation of miR-491-5p promotes neovascularization after traumatic brain injury. Neural regeneration research. vol 17. issue 3. 2021-11-02. PMID:34380897. |
cell counting kit 8 (cck-8) assay, flow cytometry, and 2?,7?-dichlorofluorescein diacetate (dcfh-da) assay results confirmed that the downregulation of mir-491-5p increased brain microvascular endothelial cell viability, reduced cell apoptosis, and alleviated oxidative stress under oxygen-glucose deprivation conditions. |
2021-11-02 |
2023-08-13 |
mouse |
| Ze Li, Junyi Jiang, Xiaohua Yi, Guoyan Wang, Shuhui Wang, Xiuzhu Su. miR-18b regulates the function of rabbit ovary granulosa cells. Reproduction, fertility, and development. vol 33. issue 5. 2021-10-29. PMID:33641714. |
furthermore, the effects of mir-18b on the proliferation, cell cycle and apoptosis of gcs were investigated using a cell counting kit (cck-8) proliferation assay, detection of annexin v-fluorescein isothiocyanate apoptosis, flow cytometry and quantitative polymerase chain reaction. |
2021-10-29 |
2023-08-13 |
rabbit |
| Zhifeng Shi, Zhixiong Zheng, Xiaodan Lin, Hengzhang M. Long Noncoding RNA MALAT1 Regulates the Progression of Atherosclerosis by miR-330-5p/NF-κB Signal Pathway. Journal of cardiovascular pharmacology. vol 78. issue 2. 2021-10-29. PMID:34554676. |
flow cytometry assay, cell counting kit 8 assay, triglyceride, and total cholesterol detection assays were used to detect the apoptosis, viability, and lipid indexes of thp-1 macrophages-derived foam cells. |
2021-10-29 |
2023-08-13 |
mouse |
| Pan Ji, Xuyang Song, Zhiping L. Knockdown of circ_0004104 Alleviates Oxidized Low-Density Lipoprotein-Induced Vascular Endothelial Cell Injury by Regulating miR-100/TNFAIP8 Axis. Journal of cardiovascular pharmacology. vol 78. issue 2. 2021-10-29. PMID:34554678. |
vec apoptosis rate was assessed using flow cytometry, and caspase-3 activity was measured using a caspase-3 assay kit. |
2021-10-29 |
2023-08-13 |
Not clear |
| Meng Hou, Chao Li, Shunbin Don. LINC00963/miR-4458 regulates the effect of oxaliplatin in gastric cancer by mediating autophagic flux through targeting of ATG16L1. Scientific reports. vol 11. issue 1. 2021-10-29. PMID:34697403. |
a caspase 3/7 activity kit and flow cytometry were used to detect the apoptosis rate. |
2021-10-29 |
2023-08-13 |
Not clear |
| Wei-Na Chai, Yi-Fan Wu, Zhi-Min Wu, Yan-Feng Xie, Quan-Hong Shi, Wei Dan, Yan Zhan, Jian-Jun Zhong, Wei Tang, Xiao-Chuan Sun, Li Jian. Neat1 decreases neuronal apoptosis after oxygen and glucose deprivation. Neural regeneration research. vol 17. issue 1. 2021-10-22. PMID:34100452. |
differences in the cell viability and apoptosis rate between groups were detected by cell-counting kit 8 assay and terminal deoxynucleotidyl transferase dutp nick-end labeling assay, respectively. |
2021-10-22 |
2023-08-13 |
mouse |
| Jens Wagenblast, Daniel Hirth, Laura Thron, Christoph Arnoldner, Marc Diensthuber, Timo Stöver, Markus Hambe. Effects of the Polo-like-kinase-1-inhibitor BI2536 in squamous cell carcinoma cell lines of the head and neck. Oncology letters. vol 4. issue 1. 2021-10-21. PMID:22807983. |
growth inhibitory and proapoptotic effects were measured quantitatively using cytohistology and a human apoptosis array kit. |
2021-10-21 |
2023-08-12 |
human |
| Martin Leinung, Clemens Cuny, Marc Diensthuber, Timo Stöver, Jens Wagenblas. Small molecules in combination with conventional chemotherapeutic drugs: Light at the end of the tunnel? Oncology letters. vol 4. issue 5. 2021-10-21. PMID:23162648. |
growth inhibitory and proapoptotic effects were measured quantitatively using cytohistology and the human apoptosis array kit. |
2021-10-21 |
2023-08-12 |
human |
| Shu Gong, Zhonghua Tao, Xiaoyan Liu, Lin Ga. An underlying prognosis predictor of hepatocellular carcinoma: Oncoprotein 18. Biomedical reports. vol 2. issue 1. 2021-10-21. PMID:24649074. |
flow cytometry was used to analyze cell apoptosis by fitc-annexin v and propidium iodide (pi) apoptosis assay kit. |
2021-10-21 |
2023-08-12 |
Not clear |
| Ling-Yun Zhou, Fang-Yuan Chen, Li-Jing Shen, Hai-Xia Wan, Ji-Hua Zhon. Arsenic trioxide induces apoptosis in the THP1 cell line by downregulating EVI-1. Experimental and therapeutic medicine. vol 8. issue 1. 2021-10-21. PMID:24944602. |
thp-1 cells were treated with different concentrations of ato (0, 1, 3 and 5 μm) for 24, 48 or 72 h, then tested for cell viability by cck-8 kit, cell morphology by cytospin smear, cell apoptosis by flow cytometry, evi-1 mrna expression by reverse transcription polymerase chain reaction (rt-pcr) and protein quantity by western blot. |
2021-10-21 |
2023-08-13 |
Not clear |
| Bo Yi, Xiaorong Hu, Zhongyuan Wen, Ting Zhang, Yuli Ca. Exendin-4, a glucagon-like peptide-1 receptor agonist, inhibits hyperglycemia-induced apoptosis in myocytes by suppressing receptor for advanced glycation end products expression. Experimental and therapeutic medicine. vol 8. issue 4. 2021-10-21. PMID:25187821. |
cell apoptosis was detected using an annexin v-fluorescein isothiocyanate/propidium iodide kit, and cell viability was measured using an mtt assay. |
2021-10-21 |
2023-08-13 |
rat |
| Yu-Guo Yuan, He-Qing Cai, Jia-Lin Wang, Ayman Mesalam, Abu Musa Md Talimur Reza, Ling Li, Li Chen, Chen Qia. Graphene Oxide-Silver Nanoparticle Nanocomposites Induce Oxidative Stress and Aberrant Methylation in Caprine Fetal Fibroblast Cells. Cells. vol 10. issue 3. 2021-10-21. PMID:33808775. |
in brief, the proliferation and apoptosis rate of go-agnp-treated cffcs (4 and 8 µg/ml of go-agnps) were measured using the cell-counting kit (cck-8) assay and the annexin v/propidium iodide (pi) assay, respectively. |
2021-10-21 |
2023-08-13 |
Not clear |