| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Hua'an Zhang, Wanhao Ye, Yingpu L. The effect of MiR-497 on the expression of target genes BCL-2 and LC3B on cardiomyocytes injured by hypoxia/reoxygenation. Cellular and molecular biology (Noisy-le-Grand, France). vol 68. issue 5. 2022-08-27. PMID:36029500. |
by detecting apoptosis, the kit was used to detect cell activity and rt-pcr detection. |
2022-08-27 |
2023-08-14 |
Not clear |
| Zhuoran Wang, Mengran Yao, Leyu Jiang, Lingyan Wang, Yuqiao Yang, Quan Wang, Xi Qian, Yu Zhao, Jinqiao Qia. Dexmedetomidine attenuates myocardial ischemia/reperfusion-induced ferroptosis via AMPK/GSK-3β/Nrf2 axis. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie. vol 154. 2022-08-21. PMID:35988428. |
cell viability and apoptosis were detected using cell counting kit 8 (cck-8), and av/pi dual staining respectively. |
2022-08-21 |
2023-08-14 |
rat |
| Hui Yu, Lan Mi, Weimin Zhang, Yingying Ye, Miaomiao Li, Dingyao Hu, Jiaowu Cao, Dedao Wang, Xiaogan Wang, Ning Ding, Yuqin Song, Jun Zh. Ibrutinib combined with low-dose HDAC inhibitor chidamide synergistically enhances the anti-tumor effect in B-cell lymphoma. Hematological oncology. 2022-08-17. PMID:35975476. |
using cell viability assay, cell cycle and apoptosis kit, we demonstrated an evident synergistic action of ibrutinib and chidamide in inhibiting tumor cell proliferation and motility. |
2022-08-17 |
2023-08-14 |
mouse |
| Jiawen Yong, Sabine Groeger, Julia von Bremen, Sabine Ru. Ciliary Neurotrophic Factor (CNTF) and Its Receptors Signal Regulate Cementoblasts Apoptosis through a Mechanism of ERK1/2 and Caspases Signaling. International journal of molecular sciences. vol 23. issue 15. 2022-08-12. PMID:35955469. |
the apoptosis ratio was measured with an annexin-v fitc/pi kit. |
2022-08-12 |
2023-08-14 |
Not clear |
| Yuqi Huang, Li Tian, Yan Liu, Jiangwei Liu, Jianzhao Huan. Ferulic Acid Protects Endothelial Cells from Hypoxia-Induced Injury by Regulating MicroRNA-92a. Applied bionics and biomechanics. vol 2022. 2022-08-12. PMID:35959508. |
the level of microrna-92 a (mir-92 a) was detected by qrt-pcr.. then, the assays of flow cytometry and the annexin v/pi staining kit were applied to value the impact of fa on hypoxia-induced cell proliferation, cell cycle distribution, and apoptosis. |
2022-08-12 |
2023-08-14 |
Not clear |
| Xu Zhao, Dongge Qiao, Dongsheng Guan, Kun Wang, Yinglin Cu. Chrysophanol Ameliorates Hemin-Induced Oxidative Stress and Endoplasmic Reticulum Stress by Regulating MicroRNA-320-5p/Wnt3a Pathway in HT22 Cells. Oxidative medicine and cellular longevity. vol 2022. 2022-08-08. PMID:35936221. |
the cell viability, apoptosis, er stress, and oxidative stress were evaluated by conducting the cell counting kit-8 (cck-8), tdt-mediated dutp nick end labeling (tunel) staining assays, western blot, and corresponding kit, respectively. |
2022-08-08 |
2023-08-14 |
Not clear |
| Mito Kanatsu-Shinohara, Honda Naoki, Takashi Tanaka, Misako Tatehana, Takako Kikkawa, Noriko Osumi, Takashi Shinohar. Regulation of male germline transmission patterns by the Trp53-Cdkn1a pathway. Stem cell reports. 2022-08-05. PMID:35931081. |
apoptosis of kit |
2022-08-05 |
2023-08-14 |
mouse |
| Hamed Manoochehri, Akram Jalali, Hamid Tanzadehpanah, Amir Taherkhani, Rezvan Najaf. Aptamer-conjugated nanoliposomes containing COL1A1 siRNA sensitize CRC cells to conventional chemotherapeutic drugs. Colloids and surfaces. B, Biointerfaces. vol 218. 2022-07-29. PMID:35905589. |
cell viability, chemosensitivity and apoptosis were determined by mtt assay and annexin/pi kit. |
2022-07-29 |
2023-08-14 |
Not clear |
| Marianna Oláh, Enikő Farkas, Inna Székács, Robert Horvath, András Székác. Cytotoxic effects of Roundup Classic and its components on NE-4C and MC3T3-E1 cell lines determined by biochemical and flow cytometric assays. Toxicology reports. vol 9. 2022-07-25. PMID:35875257. |
higher sensitivity differences were detected in the viability test by flow cytometry (7-9-fold), than by the mtt assay (1.5-3-fold); in the genotoxicity test by the comet assay (3.5-403-fold), than by the dna-damage test (9.3-158-fold); and in the apoptosis test by the annexin v dead cell kit (1.1-12.7-fold), than by the caspase 3/7 kit (1-6.5-fold). |
2022-07-25 |
2023-08-14 |
Not clear |
| Mei-Zhu Wu, Yun Xiang, Cui-Yu Bao, Tao Li. [Protective effect of salidroside on high fat-induced apoptosis in H9c2 cardiomyocytes through AMPK/mTOR/p70S6K pathway]. Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica. vol 47. issue 14. 2022-07-19. PMID:35850842. |
the study explored the effect of salidroside(sal) on high fat-induced apoptosis in h9 c2 cardiomyocytes based on ampk/mtor/p70 s6 k pathway.h9 c2 cardiomyocytes were cultured in vitro and the lipotoxicity model of h9 c2 cardiomyocytes was constructed by 0.2 mmol·l~(-1) palmitic acid(pa) treatment for 24 hours.the cells were divided into control group, pa group, and sal group(20 μmol·l~(-1)).cell proliferation was detected with cell proliferation kit i(mtt) assay after sal and pa treatment.dihydroethidium(dhe) probe, annexin v-fitc/pi kit, and jc-1 probe were used to estimate reactive oxygen species(ros) level, cell apoptosis, and mitochondrial membrane potential(mmp) change, respectively.the expression levels of p-ampk/ampk, p-mtor/mtor, p-p70 s6 k/p70 s6 k and apoptosis-related proteins bax, bcl-2, and cleaved caspase-3 were investigated with western blot.the mrna levels of ampk, mtor and p70 s6 k were determined by quantitative reverse transcription-polymerase chain reaction(qrt-pcr).results:: showed that compared with control group, pa group had decreased cell proliferation ability, mmp, bcl-2 protein expression and ampk protein and mrna expression, while increased ros level, bax and cleaved caspase-3 protein expression, and mtor and p70 s6 k mrna and protein expression, and the difference was statistically significant(p<0.05, p<0.01).compared with pa group, sal improved cell proliferation ability, mmp level, bcl-2 protein expression, and ampk mrna and protein expression, while down-regulated ros level, cell apoptosis, bax and cleaved caspase-3 protein expression, and mtor and p70 s6 k mrna and protein expression, and the difference was statistically significant(p<0.05, p<0.01).in conclusion, sal exerted protective effects on high fat-induced lipotoxicity of h9 c2 cardiomyocytes, alleviated the oxidative stress injury and reduced cell apoptosis via regulating ampk/mtor/p70 s6 k signaling pathway. |
2022-07-19 |
2023-08-14 |
Not clear |
| Jinjin Yuan, Zongwen Liu, Junqi Liu, Ruitai Fa. Circ_0060055 Promotes the Growth, Invasion, and Radioresistance of Glioblastoma by Targeting MiR-197-3p/API5 Axis. Neurotoxicity research. 2022-07-18. PMID:35849320. |
gbm cell proliferation, apoptosis, and invasion were assessed using cell counting kit 8 assay, colony formation assay, edu assay, flow cytometry, and transwell assay. |
2022-07-18 |
2023-08-14 |
human |
| Xiaoli Gao, Qiaoli Yang, Shengwei Zhang, Xiaoyu Huang, Zunqiang Yan, Pengfei Wang, Shuangbao Gu. LncRNA ALDB-898 modulates intestinal epithelial cell damage caused by Clostridium perfringens type C in piglet by regulating ssc-miR-122-5p/OCLN signaling. Molecular immunology. vol 149. 2022-07-14. PMID:35834877. |
cytometry kit 8 (cck-8), lactate dehydrogenase (ldh), real-time quantitative polymerase chain reaction (rt-qpcr), western blot, flow cytometry and fluorescein isothiocyanate-dextran 4 (fitc-dextran 4) flux assays were performed to study the effect of aldb-898 and ssc-mir-122-5p in apoptosis, inflammation and intestinal barrier damage and inflammatory in ipec-j2 cells induced by cpb2. |
2022-07-14 |
2023-08-14 |
Not clear |
| Bin Zhao, Gaofeng Qiao, Jianhua Li, Yukun Wang, XiaoDong Li, Hua Zhang, Lu Zhan. TRIM36 suppresses cell growth and promotes apoptosis in human esophageal squamous cell carcinoma cells by inhibiting Wnt/β-catenin signaling pathway. Human cell. 2022-06-29. PMID:35768649. |
cell proliferation was examined by cell counting kit (cck)-8 assay, while cell cycle distribution and cell apoptosis was assessed via flow cytometry analysis. |
2022-06-29 |
2023-08-14 |
mouse |
| Peng Tu, Sujuan Yan, Fengping Zhan. Circ_0005925 Promotes Granulosa Cell Growth by Targeting MiR-324-3p to Upregulate MAP2K6 in Polycystic Ovary Syndrome. Biochemical genetics. 2022-06-11. PMID:35689709. |
cell proliferation and apoptosis were determined using cell counting kit 8 assay, edu assay, colony formation assay, and flow cytometry. |
2022-06-11 |
2023-08-14 |
Not clear |
| Fang Zhao, Shucheng Li, Jingjing Liu, Juan Wang, Bo Yan. Long non-coding RNA TRIM52-AS1 sponges microRNA-577 to facilitate diffuse large B cell lymphoma progression via increasing TRIM52 expression. Human cell. 2022-06-08. PMID:35676608. |
further, cell counting kit (cck)-8, edu staining, and flow cytometry analyses were performed to assess the effect of trim52-as1 on dlbcl cell proliferation, apoptosis, and cell cycle. |
2022-06-08 |
2023-08-14 |
Not clear |
| Yuanyong Wang, Zhaoyang Wang, Changjian Shao, Guofang Lu, Mei Xie, Jian Wang, Hongtao Duan, Xiaofei Li, Wanpeng Yu, Weixun Duan, Xiaolong Ya. Melatonin may suppress lung adenocarcinoma progression via regulation of the circular non-coding RNA hsa_circ_0017109/miR-135b-3p/TOX3 axis. Journal of pineal research. 2022-06-06. PMID:35661247. |
also, cell apoptosis was detected using a cell apoptosis kit, and protein production was identified by western blot. |
2022-06-06 |
2023-08-14 |
human |
| Kamila Białkowska, Piotr Komorowski, Rafael Gomez-Ramirez, Francisco Javier de la Mata, Maria Bryszewska, Katarzyna Miłowsk. Interaction of Cationic Carbosilane Dendrimers and Their siRNA Complexes with MCF-7 Cells Cultured in 3D Spheroids. Cells. vol 11. issue 10. 2022-05-28. PMID:35626734. |
cytotoxicity of dendrimers and dendriplexes was measured using the live-dead test and annexin v-fitc apoptosis detection kit (flow cytometry). |
2022-05-28 |
2023-08-13 |
Not clear |
| Wei Zhang, Chunmei Shi, Qian Xu, Xinfeng Chen, Hua Zhu, Bing Zhen. Long non-coding RNA MIR22HG suppresses cell proliferation and promotes apoptosis in prostate cancer cells by sponging microRNA-9-3p. Bioengineered. vol 13. issue 5. 2022-05-25. PMID:35611601. |
cell counting kit (cck)-8, colony formation, and tunel were conducted to determine cell viability and apoptosis. |
2022-05-25 |
2023-08-13 |
Not clear |
| Wenyan Xi, Hui Mao, Zhiwei Cui, Haoyan Yao, Ruiting Sh. Scream sound induced chronic psychological stress results in diminished ovarian reserve in adult female rat. Endocrinology. 2022-05-10. PMID:35536288. |
granulosa cell apoptosis of the ovaries was examined by in situ cell death detection kit. |
2022-05-10 |
2023-08-13 |
rat |
| Wenxuan Zhang, Ziqiang Lin, Ailing Sun, Yongyong Shi, Qingxiong Hong, Gaofeng Zha. Curcumin ameliorates the experimental diabetic peripheral neuropathy through promotion of NGF expression in rats. Chemistry & biodiversity. 2022-05-10. PMID:35538560. |
the scs apoptosis in sciatic nerves was stained using tunel kit. |
2022-05-10 |
2023-08-13 |
rat |