| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Weicai Cheng, Pengbo Luan, Xiao Ji. circUBAP2 inhibits cisplatin resistance in gastric cancer via miR-300/KAT6B axis. Anti-cancer drugs. 2022-10-07. PMID:36206113. |
the effects of circubap2 on cell viability and apoptosis were investigated by cell counting kit 8 assay and flow cytometry. |
2022-10-07 |
2023-08-14 |
Not clear |
| Huiwen Guo, Lixiang Zheng, Yepin Guo, Lu Han, Jing Yu, Fuchong La. Curculigoside Represses the Proliferation and Metastasis of Osteosarcoma via the JAK/STAT and NF-κB Signaling Pathways. Biological & pharmaceutical bulletin. vol 45. issue 10. 2022-10-02. PMID:36184504. |
in vitro, cell counting kit 8 (cck-8), flow cytometry and transwell assay were used to investigate the effects of cur on os cell proliferation, apoptosis, migration and invasion. |
2022-10-02 |
2023-08-14 |
mouse |
| Yang Cao, Juan Wang, Fang Wei, Qing Gu, Min Tian, Hong-Bin L. Tert-butylhydroquinone protects the retina from oxidative stress in STZ-induced diabetic rats via the PI3K/Akt/eNOS pathway. European journal of pharmacology. 2022-09-29. PMID:36174669. |
we also utilized the tunel kit for detecting retinal cell apoptosis. |
2022-09-29 |
2023-08-14 |
rat |
| Junqi Fu, Jun Peng, Guolong T. Knockdown MTDH Inhibits Glioma Proliferation and Migration and Promotes Apoptosis by Downregulating MYBL2. Mediators of inflammation. vol 2022. 2022-09-22. PMID:36133745. |
cell counting kit 8 (cck-8) was used to detect cell proliferation, and flow cytometry was applied to measure cell apoptosis. |
2022-09-22 |
2023-08-14 |
Not clear |
| Zhao Yang, Xuming Yang, Mingchun A. Polyphyllin I improves myocardial damage in coronary artery disease via modulating lipid metabolism and myocardial apoptosis. Journal of biochemical and molecular toxicology. 2022-09-19. PMID:36120828. |
the apoptosis of myocardial cells was assessed by annexin v-fitc/pi kit and tunel staining. |
2022-09-19 |
2023-08-14 |
rat |
| Zhao Yang, Xuming Yang, Mingchun A. Polyphyllin I improves myocardial damage in coronary artery disease via modulating lipid metabolism and myocardial apoptosis. Journal of biochemical and molecular toxicology. 2022-09-19. PMID:36120828. |
the apoptosis of myocardial cells was assessed by annexin v-fitc/pi kit and tunel staining. |
2022-09-19 |
2023-08-14 |
rat |
| Suleyman Ilhan, Çisil Çamli Pulat, Ferdi Oguz, Hakan Bektaş, Emre Menteşe, Harika Atmac. Design and synthesis of benzimidazole derivatives as apoptosis-inducing agents by targeting Bcl-2 protein. Molecular diversity. 2022-09-06. PMID:36065037. |
pi annexin v apoptosis detection kit was used to detect apoptosis. |
2022-09-06 |
2023-08-14 |
Not clear |
| S Chen, J Yang, F Wang, X Gao, Qiang Liu, Qian Liu, Y Zhang, Y Y. Rapamycin Enhanced Sensitivity of HT-29 Cells to 5-Fluororacil by Promoting Autophagy. Bulletin of experimental biology and medicine. 2022-09-05. PMID:36063298. |
the proliferation and apoptosis of treated cells were evaluated using cell counting kit-8 kit and flow cytometry, respectively. |
2022-09-05 |
2023-08-14 |
Not clear |
| Hua'an Zhang, Wanhao Ye, Yingpu L. The effect of MiR-497 on the expression of target genes BCL-2 and LC3B on cardiomyocytes injured by hypoxia/reoxygenation. Cellular and molecular biology (Noisy-le-Grand, France). vol 68. issue 5. 2022-08-27. PMID:36029500. |
by detecting apoptosis, the kit was used to detect cell activity and rt-pcr detection. |
2022-08-27 |
2023-08-14 |
Not clear |
| Zhuoran Wang, Mengran Yao, Leyu Jiang, Lingyan Wang, Yuqiao Yang, Quan Wang, Xi Qian, Yu Zhao, Jinqiao Qia. Dexmedetomidine attenuates myocardial ischemia/reperfusion-induced ferroptosis via AMPK/GSK-3β/Nrf2 axis. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie. vol 154. 2022-08-21. PMID:35988428. |
cell viability and apoptosis were detected using cell counting kit 8 (cck-8), and av/pi dual staining respectively. |
2022-08-21 |
2023-08-14 |
rat |
| Hui Yu, Lan Mi, Weimin Zhang, Yingying Ye, Miaomiao Li, Dingyao Hu, Jiaowu Cao, Dedao Wang, Xiaogan Wang, Ning Ding, Yuqin Song, Jun Zh. Ibrutinib combined with low-dose HDAC inhibitor chidamide synergistically enhances the anti-tumor effect in B-cell lymphoma. Hematological oncology. 2022-08-17. PMID:35975476. |
using cell viability assay, cell cycle and apoptosis kit, we demonstrated an evident synergistic action of ibrutinib and chidamide in inhibiting tumor cell proliferation and motility. |
2022-08-17 |
2023-08-14 |
mouse |
| Jiawen Yong, Sabine Groeger, Julia von Bremen, Sabine Ru. Ciliary Neurotrophic Factor (CNTF) and Its Receptors Signal Regulate Cementoblasts Apoptosis through a Mechanism of ERK1/2 and Caspases Signaling. International journal of molecular sciences. vol 23. issue 15. 2022-08-12. PMID:35955469. |
the apoptosis ratio was measured with an annexin-v fitc/pi kit. |
2022-08-12 |
2023-08-14 |
Not clear |
| Yuqi Huang, Li Tian, Yan Liu, Jiangwei Liu, Jianzhao Huan. Ferulic Acid Protects Endothelial Cells from Hypoxia-Induced Injury by Regulating MicroRNA-92a. Applied bionics and biomechanics. vol 2022. 2022-08-12. PMID:35959508. |
the level of microrna-92 a (mir-92 a) was detected by qrt-pcr.. then, the assays of flow cytometry and the annexin v/pi staining kit were applied to value the impact of fa on hypoxia-induced cell proliferation, cell cycle distribution, and apoptosis. |
2022-08-12 |
2023-08-14 |
Not clear |
| Xu Zhao, Dongge Qiao, Dongsheng Guan, Kun Wang, Yinglin Cu. Chrysophanol Ameliorates Hemin-Induced Oxidative Stress and Endoplasmic Reticulum Stress by Regulating MicroRNA-320-5p/Wnt3a Pathway in HT22 Cells. Oxidative medicine and cellular longevity. vol 2022. 2022-08-08. PMID:35936221. |
the cell viability, apoptosis, er stress, and oxidative stress were evaluated by conducting the cell counting kit-8 (cck-8), tdt-mediated dutp nick end labeling (tunel) staining assays, western blot, and corresponding kit, respectively. |
2022-08-08 |
2023-08-14 |
Not clear |
| Mito Kanatsu-Shinohara, Honda Naoki, Takashi Tanaka, Misako Tatehana, Takako Kikkawa, Noriko Osumi, Takashi Shinohar. Regulation of male germline transmission patterns by the Trp53-Cdkn1a pathway. Stem cell reports. 2022-08-05. PMID:35931081. |
apoptosis of kit |
2022-08-05 |
2023-08-14 |
mouse |
| Hamed Manoochehri, Akram Jalali, Hamid Tanzadehpanah, Amir Taherkhani, Rezvan Najaf. Aptamer-conjugated nanoliposomes containing COL1A1 siRNA sensitize CRC cells to conventional chemotherapeutic drugs. Colloids and surfaces. B, Biointerfaces. vol 218. 2022-07-29. PMID:35905589. |
cell viability, chemosensitivity and apoptosis were determined by mtt assay and annexin/pi kit. |
2022-07-29 |
2023-08-14 |
Not clear |
| Marianna Oláh, Enikő Farkas, Inna Székács, Robert Horvath, András Székác. Cytotoxic effects of Roundup Classic and its components on NE-4C and MC3T3-E1 cell lines determined by biochemical and flow cytometric assays. Toxicology reports. vol 9. 2022-07-25. PMID:35875257. |
higher sensitivity differences were detected in the viability test by flow cytometry (7-9-fold), than by the mtt assay (1.5-3-fold); in the genotoxicity test by the comet assay (3.5-403-fold), than by the dna-damage test (9.3-158-fold); and in the apoptosis test by the annexin v dead cell kit (1.1-12.7-fold), than by the caspase 3/7 kit (1-6.5-fold). |
2022-07-25 |
2023-08-14 |
Not clear |
| Mei-Zhu Wu, Yun Xiang, Cui-Yu Bao, Tao Li. [Protective effect of salidroside on high fat-induced apoptosis in H9c2 cardiomyocytes through AMPK/mTOR/p70S6K pathway]. Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica. vol 47. issue 14. 2022-07-19. PMID:35850842. |
the study explored the effect of salidroside(sal) on high fat-induced apoptosis in h9 c2 cardiomyocytes based on ampk/mtor/p70 s6 k pathway.h9 c2 cardiomyocytes were cultured in vitro and the lipotoxicity model of h9 c2 cardiomyocytes was constructed by 0.2 mmol·l~(-1) palmitic acid(pa) treatment for 24 hours.the cells were divided into control group, pa group, and sal group(20 μmol·l~(-1)).cell proliferation was detected with cell proliferation kit i(mtt) assay after sal and pa treatment.dihydroethidium(dhe) probe, annexin v-fitc/pi kit, and jc-1 probe were used to estimate reactive oxygen species(ros) level, cell apoptosis, and mitochondrial membrane potential(mmp) change, respectively.the expression levels of p-ampk/ampk, p-mtor/mtor, p-p70 s6 k/p70 s6 k and apoptosis-related proteins bax, bcl-2, and cleaved caspase-3 were investigated with western blot.the mrna levels of ampk, mtor and p70 s6 k were determined by quantitative reverse transcription-polymerase chain reaction(qrt-pcr).results:: showed that compared with control group, pa group had decreased cell proliferation ability, mmp, bcl-2 protein expression and ampk protein and mrna expression, while increased ros level, bax and cleaved caspase-3 protein expression, and mtor and p70 s6 k mrna and protein expression, and the difference was statistically significant(p<0.05, p<0.01).compared with pa group, sal improved cell proliferation ability, mmp level, bcl-2 protein expression, and ampk mrna and protein expression, while down-regulated ros level, cell apoptosis, bax and cleaved caspase-3 protein expression, and mtor and p70 s6 k mrna and protein expression, and the difference was statistically significant(p<0.05, p<0.01).in conclusion, sal exerted protective effects on high fat-induced lipotoxicity of h9 c2 cardiomyocytes, alleviated the oxidative stress injury and reduced cell apoptosis via regulating ampk/mtor/p70 s6 k signaling pathway. |
2022-07-19 |
2023-08-14 |
Not clear |
| Jinjin Yuan, Zongwen Liu, Junqi Liu, Ruitai Fa. Circ_0060055 Promotes the Growth, Invasion, and Radioresistance of Glioblastoma by Targeting MiR-197-3p/API5 Axis. Neurotoxicity research. 2022-07-18. PMID:35849320. |
gbm cell proliferation, apoptosis, and invasion were assessed using cell counting kit 8 assay, colony formation assay, edu assay, flow cytometry, and transwell assay. |
2022-07-18 |
2023-08-14 |
human |
| Xiaoli Gao, Qiaoli Yang, Shengwei Zhang, Xiaoyu Huang, Zunqiang Yan, Pengfei Wang, Shuangbao Gu. LncRNA ALDB-898 modulates intestinal epithelial cell damage caused by Clostridium perfringens type C in piglet by regulating ssc-miR-122-5p/OCLN signaling. Molecular immunology. vol 149. 2022-07-14. PMID:35834877. |
cytometry kit 8 (cck-8), lactate dehydrogenase (ldh), real-time quantitative polymerase chain reaction (rt-qpcr), western blot, flow cytometry and fluorescein isothiocyanate-dextran 4 (fitc-dextran 4) flux assays were performed to study the effect of aldb-898 and ssc-mir-122-5p in apoptosis, inflammation and intestinal barrier damage and inflammatory in ipec-j2 cells induced by cpb2. |
2022-07-14 |
2023-08-14 |
Not clear |