| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Xiaopeng Peng, Haoyao Yuan, Guangtian Chen, Yuliang Guo, Qiuer Liang, Qiming Chen, Weidong Ca. Investigation on the effect of ulinastatin on the apoptosis of vascular smooth muscle cells in rats with aortic dissection based on the Sirt1/FoxO3a pathway. Cellular and molecular biology (Noisy-le-Grand, France). vol 69. issue 13. 2023-12-30. PMID:38158682. |
for this purpose a rat model of aortic dissection (ad) was constructed by giving drinking water containing 0.08% β-aminopropionitrile (bapn) to rats, he staining was used to observe the pathological changes of the aorta in ad rats; the diseased blood vessels of ad rats were taken for primary culture and passage of vsmcs, the morphology of vsmcs was observed, and vsmcs were identify with immunofluorescence staining; vsmcs were treated with culture media containing 0, 1000, 2000, 3000, 4000, 5000, 6000, 7000 u/ml ulinastatin, and mtt kit was used to determine the effect of ulinastatin on vsmc proliferation in ad rats; the vsmc of ad rats were divided into blank group (normal culture), ulinastatin group (medium containing 5000 u/ml ulinastatin), sirt1 inhibitor group (medium containing 1 μmol/l ex527), ulinastatin + sirt1 inhibitor group (medium containing 5000 u/ml ulinastatin, 1 μmol/l ex527), flow cytometry was used to detect the vsmc apoptosis in each group, wb was used to detect the expression of vsmc apoptosis-related proteins and sirt1/foxo3a pathway-related proteins in each group. |
2023-12-30 |
2024-01-05 |
rat |
| Wei-Yu Guo, Ding-Yan Lu, Zhi-Zhong Guan, Lin Zheng, Shuai-Shuai Chen, Ting Li. Periploca forrestii Schltr. ameliorate liver injury caused by fluorosis in rat. Ecotoxicology and environmental safety. vol 270. 2023-12-19. PMID:38113798. |
additionally, cell apoptosis rate was determined using the annexin v-fitc/pi cell apoptosis assay kit. |
2023-12-19 |
2023-12-23 |
human |
| Rui Zhang, Ya Li, Jin Zhan. Molecular mechanisms of pelvic organ prolapse influenced by FBLN5 via FOSL1/miR-222/MEIS1/COL3A1 axis. Cellular signalling. 2023-12-06. PMID:38056607. |
cellular cytoskeletal changes were examined via phalloidin staining, intracellular ros levels with a ros kit, cell apoptosis through flow cytometry, and cell senescence using β-galactosidase staining. |
2023-12-06 |
2023-12-10 |
human |
| Aysun Ökçesiz Hacıseyitoğlu, Tuğbagül Çal Doğan, Sevtap Aydın Dilsiz, Hande Canpınar, Ayşe Eken, Ülkü Ündeğer Bucurga. Pitavastatin induces caspase-mediated apoptotic death through oxidative stress and DNA damage in combined with cisplatin in human cervical cancer cell line. Journal of applied toxicology : JAT. 2023-12-06. PMID:38053498. |
pita-induced apoptosis, an increased proportion of sub g1 cells, was monitored, and also, it increased the expression of active caspase-9 and caspase-3 and upregulated cleaved poly adenosine diphosphate ribose polymerase (parp) by western blotting and caspase 3/8/9 multiple assay kit. |
2023-12-06 |
2023-12-10 |
human |
| Xin Wei, Jinliang Ni, Lin Yuan, Xueliang L. Hematoporphyrin derivative photodynamic therapy induces apoptosis and suppresses the migration of human esophageal squamous cell carcinoma cells by regulating the PI3K/AKT/mTOR signaling pathway. Oncology letters. vol 27. issue 1. 2023-11-30. PMID:38034489. |
an annexin v-fitc/pi kit was used to determine cell apoptosis rates. |
2023-11-30 |
2023-12-10 |
human |
| Chao Jiang, Youjun Liu, Weigong Zhao, Yimin Yang, Zhiwei Ren, Xiaohui Wang, Dingjun Hao, Heng Du, Si Yi. microRNA-365 attenuated intervertebral disc degeneration through modulating nucleus pulposus cell apoptosis and extracellular matrix degradation by targeting EFNA3. Journal of cellular and molecular medicine. 2023-11-27. PMID:38009813. |
meanwhile, np cells apoptosis was measured through propidium iodide (pi)-annexinv-fluorescein isothiocyanate (fitc) apoptosis detection kit. |
2023-11-27 |
2023-11-29 |
rat |
| Fan Zhou, Dong Liu, Junwei Ye, Bingqi L. Circ_0006944 aggravates LPS-induced HK2 cell injury via modulating miR-205-5p/UBL4A pathway. Autoimmunity. vol 56. issue 1. 2023-11-24. PMID:37994026. |
cell counting kit 8 assay, edu assay and flow cytometry were exploited to estimate cell proliferation and apoptosis. |
2023-11-24 |
2023-11-28 |
human |
| Li Zhao, Hongtao Shi, Fan Zhang, Honghong Xue, Qinghua Ha. Hederagenin protects against myocardial ischemia-reperfusion injury via attenuating ALOX5-mediated ferroptosis. Naunyn-Schmiedeberg's archives of pharmacology. 2023-11-13. PMID:37955689. |
cell counting kit (cck)-8, terminal deoxynucleotidyl transferase-mediated dutp nick-end labeling (tunel), and flow cytometry assays were performed to assess cell viability and apoptosis. |
2023-11-13 |
2023-11-20 |
rat |
| Qian Wu, Wenrui Yang, Yanju Bi, Yujie Yao, Chengzhi Li, Xiaojing L. Baicalein inhibits apoptosis and autophagy induced by chlorpyrifos exposure to kidney of Cyprinus carpio through activation of PI3K/AKT pathway. Pesticide biochemistry and physiology. vol 196. 2023-11-09. PMID:37945259. |
furthermore, cpf induced the increase of ros level and inhibition of pi3k and nrf2 pathways, which in turn triggered oxidative stress, autophagy and apoptosis in carp kidney according to western blot, rt-qpcr and kit assays. |
2023-11-09 |
2023-11-20 |
Not clear |
| Zhijing Zhou, Peng Zhan. Formononetin ameliorates the LPS-induced inflammatory response and apoptosis of neuronal cells via NF-κB/NLRP3 signaling pathway. Functional & integrative genomics. vol 23. issue 4. 2023-10-17. PMID:37847432. |
the lactate dehydrogenase (ldh) concentration and cell viability, proliferating cell nuclear antigen (pcna) fluorescence intensity, and cell apoptosis were determined using an ldh kit, cell counting kit-8 (cck-8), immunofluorescence, and flow cytometry assays, respectively. |
2023-10-17 |
2023-11-08 |
Not clear |
| Sirui Wang, Hua Duan, Sha Wang, Zhengchen Guo, Qi Li. miR-141-3p Regulates the Proliferation and Apoptosis of Endometrial-Myometrial Interface Smooth Muscle Cells in Adenomyosis Via JAK2/STAT3 Pathway. Biochemical genetics. 2023-10-12. PMID:37828348. |
the cell counting kit 8 assay and flow cytometry analysis were used to evaluate the proliferation and apoptosis of emi smcs. |
2023-10-12 |
2023-10-15 |
Not clear |
| Yang An, Dan Xu, Lei Yuan, Ying We. Circ_0059662 exerts a positive role in oxygen-glucose deprivation/reoxygenation-induced SK-N-SH cell injury. Experimental brain research. 2023-10-10. PMID:37815551. |
cell counting kit 8 assay, edu assay and flow cytometry were utilized to detect cell proliferation and apoptosis. |
2023-10-10 |
2023-10-15 |
Not clear |
| Hefei Huang, Tianlun Wang, Luopei Wang, Yan Huang, Weili Li, Jin'e Wang, Yuanlang Hu, Zhiyong Zho. Saponins of Panax japonicus ameliorates cardiac aging phenotype in aging rats by enhancing basal autophagy through AMPK/mTOR/ULK1 pathway. Experimental gerontology. 2023-10-05. PMID:37797916. |
tunel kit was used to detect apoptosis level of cardiomyocyte, and western blot was used to evaluate autophagy-related proteins as well as ampk/mtor/ulk1 pathway-related markers. |
2023-10-05 |
2023-10-07 |
mouse |
| Dongju Zhu, Xiang W. Resveratrol Inhibits circ_0074371-related Pathway to Alleviate Sepsis-induced Acute Kidney Injury. Biochemical genetics. 2023-09-21. PMID:37730967. |
cell proliferation and apoptosis were detected by cell counting kit 8 assay, edu assay and flow cytometry. |
2023-09-21 |
2023-10-07 |
Not clear |
| Hui Hui, Gaowa Zhao, Mingliang Du, Qin Y. circABCA3 knockdown relieves hypoxia-induced human cardiac microvascular endothelial cell dysfunction by targeting the miR-671-5p/PCSK9 axis. Cell stress & chaperones. 2023-09-07. PMID:37676419. |
cell proliferation, migration, angiogenesis, and apoptosis were measured using cell counting kit 8 assay, edu assay, transwell assay, wound healing assay, tube formation assay, and flow cytometry. |
2023-09-07 |
2023-10-07 |
human |
| Mohammad Abdelwahab, Hesham Saeed, Nefertiti Elnikhely, Hisham Nematall. Synergistic effect of Dactolisib/Lys05 combination on autophagy in A549 cells. Acta biochimica Polonica. 2023-09-07. PMID:37677845. |
these effects were corroborated by elisa, western blot, and flow cytometry using the annexin v-fitc apoptosis detection kit. |
2023-09-07 |
2023-10-07 |
Not clear |
| Huajie Mao, Runzhi Wang, Fengling Shao, Ming Zhao, Dayu Tian, Hua Xia, Ya Zha. HMGCS2 serves as a potential biomarker for inhibition of renal clear cell carcinoma growth. Scientific reports. vol 13. issue 1. 2023-09-05. PMID:37670031. |
finally, the results of cell counting kit 8 assays, colony formation assays, flow cytometry, and western blot analysis suggested that upregulation of hmgcs2 increased the expression of key tumor suppressor proteins, inhibited the proliferation of clear cell renal cell carcinoma cells and promoted cell apoptosis. |
2023-09-05 |
2023-10-07 |
human |
| Pu Li, Zhaohui Zheng, Jing Qi, Yanyao Gao, Liu Yang, Lu Li, Changjun Ga. HDAC3 improves intestinal function of mice by regulating cGAS-Sting pathway of intestinal glial cells. Molecular immunology. vol 162. 2023-09-04. PMID:37666082. |
apoptosis kit was used to determine cell apoptosis rate. |
2023-09-04 |
2023-09-07 |
mouse |
| Wei Lu, Liang Li, Li Li, Nanbian Guo, Ximiao M. Circular RNA circ_0101675 Promotes NSCLC Cell Proliferation, Migration, Invasion, Angiogenesis and Immune Evasion by Sponging miR-607/PDL1 Axis. Biochemical genetics. 2023-08-30. PMID:37646893. |
cell count kit 8 assay, colony formation assay, wound healing assay, transwell assay, tube formation assay and flow cytometry were applied to examine nsclc cell proliferation, migration, invasion, angiogenesis and apoptosis. |
2023-08-30 |
2023-09-07 |
Not clear |
| Chao Chen, Yu'e Liu, Hongxiang Wang, Xu Zhang, Yufeng Shi, Juxiang Che. FOXO1-miR-506 axis promotes chemosensitivity to temozolomide and suppresses invasiveness in glioblastoma through a feedback loop of FOXO1/miR-506/ETS1/FOXO1. Journal of Zhejiang University. Science. B. vol 24. issue 8. 2023-08-08. PMID:37551556. |
cell viability was tested by a cell counting kit-8 (cck8) kit; migration and invasion were checked by the scratching assay; apoptosis was evaluated by terminal deoxynucleotidyl transferase dutp nick-end labeling (tunel) staining and flow cytometry. |
2023-08-08 |
2023-08-14 |
mouse |