| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Bo Deng, Xuegang He, Zhaoheng Wang, Jihe Kang, Guangzhi Zhang, Lei Li, Xuewen Kan. HSP70 protects PC12 cells against TBHP-induced apoptosis and oxidative stress by activating the Nrf2/HO-1 signaling pathway. In vitro cellular & developmental biology. Animal. 2024-05-28. PMID:38807023. |
cell viability was assessed using the cck8 kit, intracellular reactive oxygen species level was evaluated using a commercial kit, cell apoptosis was detected using the annexin v-apc/7-add apoptosis detection kit, and the oxidative stress level was determined using sod and mda assay kits. |
2024-05-28 |
2024-06-03 |
Not clear |
| Xiaojie Cai, Xiao Wang, Yuping Huang, Xiaopang Ra. Molecular mechanism of wedelolactone inhibits high glucose-induced human retinal vascular endothelial cells injury through regulating miR-190 expression. Medicine. vol 103. issue 21. 2024-05-24. PMID:38788037. |
the kit detects cellular reactive oxygen species (ros), superoxide dismutase (sod), and malondialdehyde (mda) content; cell apoptosis was analyzed by flow cytometry; mir-190 expression was detected by real-time quantitative pcr (rt-qpcr). |
2024-05-24 |
2024-05-27 |
human |
| Shujiang Zhuo, Sufei Song, Chaoyi Wang, Zhe Wang, Ming Zhang, Daobin Lin, Kaili Che. Inflammatory corpuscle AIM2 facilitates macrophage foam cell formation by inhibiting cholesterol efflux protein ABCA1. Scientific reports. vol 14. issue 1. 2024-05-11. PMID:38734775. |
the intracellular cholesterol efflux rate was detected by scintillation counting; high-performance liquid chromatography (hplc) was used to detect intracellular cholesterol levels; apoptosis levels were detected by tunel kit; levels of inflammatory markers (il-1β, il-18, ros, and gsh) were detected by elisa kits; and levels of aim2 and abca1 proteins were detected by western blot. |
2024-05-11 |
2024-05-27 |
Not clear |
| Hongjuan Duan, Shaojin Li, Chuangwei Li, Lan Tian, Yun Ma, Xiaoyan Ca. Alfalfa xeno-miR159a regulates bovine mammary epithelial cell proliferation and milk protein synthesis by targeting PTPRF. Scientific reports. vol 14. issue 1. 2024-04-20. PMID:38643232. |
cell proliferation and apoptosis were detected using the cell counting kit 8 (cck-8) assay, 5-ethynyl-2'-deoxyuridine (edu) staining, western blot, and flow cytometry. |
2024-04-20 |
2024-04-23 |
cattle |
| Xin Qi, Xin Chen, Wen-Bo An, Zhi-Ming Xu, Duo-Xian Wang, Peng-Fei Luo, Yi-Xin Chen, Jiao-Jiao Ma, Zi-Yang Hu, Wei Qi, Jian-Jun Liu, Jun-Xi Li. [Ligusticum cycloprolactam inhibits IL-1β-induced apoptosis and inflammation of rat chondrocytes via HMGB1/TLR4/NF-κB signaling pathway]. Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica. vol 49. issue 4. 2024-04-15. PMID:38621908. |
annexin v-fitc/pi apoptosis detection kit was employed to examine the apoptosis of chondrocytes in each group. |
2024-04-15 |
2024-04-18 |
rat |
| Jiayu Shen, Ningning Hu, Zetian Wang, Lina Yang, Rujun Chen, Liwen Zhang, Xiaoqin Wan. Ghrelin alleviates placental dysfunction by down-regulating NF-κB phosphorylation in LPS-induced rat model of preeclampsia. European journal of pharmacology. 2024-04-09. PMID:38593930. |
in addition, the apoptosis rates of jeg-3 and htr-8/svneo trophoblast cells were determined by annexin v-fitc/pi apoptosis detection kit. |
2024-04-09 |
2024-04-12 |
human |
| Josquin Moraly, Julien Rossignol, Claire Rouzaud, Thomas Gabas, Hassiba Bouktit, Ludovic Lhermitte, Danielle Canioni, Sylvie Fraitag, Julie Bruneau, Stéphane Barete, Felipe Suarez, Thomas Ballul, Cécile Meni, Laura Polivka, Louis Terriou, David Launay, Laurence Bouillet, Caroline Gaudy-Marqueste, Marie Gousseff, Edwige Le Mouel, Antoine Neel, Dana Ranta, Roland Jaussaud, Philippe Guilpain, Laurent Frenzel, Julie Agopian, Patrice Dubreuil, Céline Greco, Sophie Dimicoli-Salazar, Mael Heiblig, Clément Gourguechon, Olivier Tournilhac, Rose-Marie Javier, Florence Castelain, Quentin Cabrera, Marie Pierre Gourin, Ewa Wierzbicka-Hainaut, Jose Miguel Torregrosa-Diaz, Cristina Bulai, Christian Lavigne, Cyrille Hoarau, Michel Arock, Gandhi Damaj, Olivier Lortholary, Olivier Hermin. Efficacy and safety of mammalian target of rapamycin inhibitors in systemic mastocytosis: A nationwide French pilot study. American journal of hematology. 2024-04-06. PMID:38581211. |
furthermore, rapamycin induces the apoptosis of kit d816v mcs selectively. |
2024-04-06 |
2024-04-10 |
Not clear |
| Yan Meng, Zhenzhen Hu, Chenyi Zhang, Hao Bai, Zhaoping Li, Xinru Guo, Liyong Che. miR-92a-3p regulates ethanol-induced apoptosis in H9c2 cardiomyocytes. Cell stress & chaperones. 2024-04-06. PMID:38582327. |
an annexin v-fluorescein isothiocyanate (fitc)/propidium iodide (pi) apoptosis detection kit and apoptosis-related antibodies were used for apoptosis detection through flow cytometry and western blotting, respectively. |
2024-04-06 |
2024-04-10 |
Not clear |
| Mengli Zhang, Bei Xu, Wei Li, Bo Yu, Huan Peng, Feng Gui, Fen Ai, Zhen Che. lncRNA CCAT2 Protects Against Cardiomyocyte Injury After Myocardial Ischemia/Reperfusion by Regulating BMI1 Expression. International heart journal. vol 65. issue 2. 2024-03-31. PMID:38556336. |
cell viability, lactate dehydrogenase (ldh) leakage, reactive oxygen species (ros) levels, mitochondrial membrane potential, and apoptosis were detected using counting kit-8, ldh assay kit, dihydroethidium assay, 5,5',6,6'-tetrachloro1,1',3,3'-tetramethylbenzimidazolylcarbocyanine iodide staining, flow cytometry, and western blotting, respectively. |
2024-03-31 |
2024-04-03 |
Not clear |
| Xiangcheng Wang, Ruilong Niu, Hao Yang, Yu Lin, Hui Hou, Hong Yan. Fibroblast activation protein promotes progression of hepatocellular carcinoma via regulating the immunity. Cell biology international. 2024-03-19. PMID:38501437. |
the cell proliferation and apoptosis were determined using the cell counting kit-8 assay kit and annexin v-fitc/pi apoptosis kit, respectively. |
2024-03-19 |
2024-03-21 |
Not clear |
| Xiangxin Kong, Denglong Cheng, Xu Xu, Yuan Zhang, Xin Li, Wanlong Pa. IFN‑γ induces apoptosis in gemcitabine‑resistant pancreatic cancer cells. Molecular medicine reports. vol 29. issue 5. 2024-03-15. PMID:38488034. |
the ifn‑γ‑induced apoptosis of panc‑1/gem cells was assessed using an apoptosis detection kit, whereas the impact of ifn‑γ on the migration of these cells was evaluated using a wound‑healing assay. |
2024-03-15 |
2024-03-17 |
Not clear |
| Tong Wang, Liying Gao, Jiahua Tan, Dongzhi Zhuoma, Ruiying Yuan, Bin Li, Shan Huan. The Neuroprotective Effect of Sophocarpine against Glutamate-Induced HT22 Cell Cytotoxicity. Journal of oleo science. vol 73. issue 3. 2024-03-03. PMID:38433000. |
mitochondrial membrane potential (mmp) and cell apoptosis were used by 5, 5', 6, 6'-tetrachloro-1, 1', 3, 3'-tetraethyl-imidacarbocyanine iodide (jc- 1) kit and terminal deoxynucleotidyl transferase-mediated dutp nick-end labeling (tunel) apoptosis assay kit, respectively. |
2024-03-03 |
2024-03-06 |
mouse |
| Chengliang Sun, Dongzhi Liu, Shunheng Gao, Mingyu Xiu, Zhaojian Zhan. Propofol Ameliorates Spinal Cord Injury Process by Mediating miR-672-3p/TNIP2 Axis. Biochemical genetics. 2024-02-21. PMID:38379038. |
cell viability and apoptosis were determined by cell counting kit 8 assay, flow cytometry, and caspase-3 activity detection. |
2024-02-21 |
2024-02-23 |
rat |
| Shenggui Xu, Weimin Lin, Wang Lin, Chengshou Lin, Weizhong Gu. Blocking TXNIP reduced IL-1β Induced chondrocyte cell inflammation. Cellular and molecular biology (Noisy-le-Grand, France). vol 69. issue 15. 2024-01-27. PMID:38279454. |
after inhibiting the expression of txnip by transfecting shtxnip into normal mouse chondrocytes, the cck-8 kit was used for detecting its effect on cell proliferation after transfection, and the effect on chondrocyte apoptosis was detected by flow cytometry. |
2024-01-27 |
2024-01-29 |
mouse |
| Yue Wu, Dan Chen, Longguang L. EXPRESS: Morinda officinalis polysaccharide promotes the osteogenic differentiation of human bone marrow-derived mesenchymal stem cells via microRNA-210-3p / scavenger receptor class A member 3. Journal of investigative medicine : the official publication of the American Federation for Clinical Research. 2024-01-24. PMID:38264863. |
cell counting kit-8 assay was employed to detect cell viability; terminal-deoxynucleoitidyl transferase mediated nick end labeling assay and flow cytometry were adopted to detect apoptosis; alkaline phosphatase (alp) activity assay kit was applied to detect alp activity; western blot was executed to quantify the expression levels of scara3, osteogenic and adipogenic differentiation markers. |
2024-01-24 |
2024-01-26 |
human |
| Chongnan Zhang, Jing Zhang, Kai Gu. Paeonol upregulates expression of tumor suppressors TNNC1 and SCARA5, exerting anti-tumor activity in non-small cell lung cancer cells. Naunyn-Schmiedeberg's archives of pharmacology. 2024-01-24. PMID:38265681. |
cell viability, caspase-3 activity, and apoptosis were evaluated using cck-8 assay, caspase-3 colorimetric assay kit, and flow cytometry analysis, respectively. |
2024-01-24 |
2024-01-26 |
Not clear |
| Jiankun Ren, Songwei Zhao, Junyu La. Triptolide restrains the growth, invasion, stemness, and glycolysis of non-small cell lung cancer cells by PFKFB2-mediated PI3K/AKT pathway. Chemical biology & drug design. vol 103. issue 1. 2024-01-17. PMID:38230789. |
cell proliferation, apoptosis, invasion, and stemness were detected by cell counting kit 8 assay, edu assay, flow cytometry, transwell assay, and sphere formation assay. |
2024-01-17 |
2024-01-19 |
Not clear |
| Peiyu Shuai, Zhihong Hu, Wei Li, Guoliang You, Zhiye Liu, Niandong Zhen. The protective role of circ_0016760 downregulation against sevoflurane‑induced neurological impairment via modulating miR‑145 expression in aged rats. Acta neurobiologiae experimentalis. vol 83. issue 4. 2024-01-15. PMID:38224284. |
the cck‑8 assay, flow cytometry, elisa, ros kit, mwm test were carried out to measure the cell viability, apoptosis, inflammation indicators, ros level, and cognitive and memory function of the rats. |
2024-01-15 |
2024-01-17 |
rat |
| Qi Liu, Linfang Li, Jingzhuang Zhao, Guangming Ren, Tongyan Lu, Yizhi Shao, Liming X. Andrographolide Alleviates Oxidative Damage and Inhibits Apoptosis Induced by IHNV Infection via CTSK/BCL2/Cytc Axis. International journal of molecular sciences. vol 25. issue 1. 2024-01-11. PMID:38203479. |
fluorescent staining was performed using a reactive oxygen species detection kit (ros) and hoechst 33342/pi double staining kit, and the mechanism of alleviation of apoptosis and oxidative stress andrographolide after ihnv infection was determined. |
2024-01-11 |
2024-01-13 |
Not clear |
| Lili Zhang, Fei Sh. LMTK2 inhibits Aβ25-35-elicited ferroptosis, oxidative stress and apoptotic damage in PC12 cells through activating Nrf2/ARE signalling pathway. Folia neuropathologica. 2024-01-04. PMID:38174688. |
with the application of cck-8, tunel, iron colorimetric assay kit and dcfh-da, the viability, apoptosis, fe 2+ and ros content in pc12 cells were assessed. |
2024-01-04 |
2024-01-06 |
cat |