| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Wai-Leng Lee, Tuan-Nan Wen, Jeng-Yuan Shiau, Lie-Fen Shyu. Differential proteomic profiling identifies novel molecular targets of paclitaxel and phytoagent deoxyelephantopin against mammary adenocarcinoma cells. Journal of proteome research. vol 9. issue 1. 2010-03-30. PMID:19894775. |
western blot analysis of marker proteins (e.g., pdi, grp78, txnd5, caspase-12, caspase-3 and parp) further verified that induction of endoplasmic reticulum stress was associated with apoptosis induced by both deoxyelephantopin and paclitaxel, but only deoxyelephantopin inhibited proteasomal proteolysis in ts/a cells. |
2010-03-30 |
2023-08-12 |
mouse |
| Yong Li, Ningning Han, Ning Gao, Rong Xu, Chenghang Sun, Diandong Li, Qiyang H. Induction of apoptosis by the angucyclinone antibiotic chemomicin in human tumor cells. Oncology reports. vol 23. issue 2. 2010-03-30. PMID:20043110. |
the apoptotic cells with chromatin condensation and annexin v staining markedly increased after the human hepatoma hepg2 was exposed to 1, or 2 microg/ml chm for 24 h. in the chm-induced apoptosis, robust increment of p53 expression, activation of caspase-3, -7, -8, -9, cleavage of parp and the phosphorylation of p38 and jnk, were detected by western blot analysis. |
2010-03-30 |
2023-08-12 |
human |
| Kazuto Okuno, Shigeru Ohta, Hirofumi Kato, Takashi Taga, Kanji Sugita, Yoshihiro Takeuch. Expression of neural stem cell markers in malignant rhabdoid tumor cell lines. Oncology reports. vol 23. issue 2. 2010-03-30. PMID:20043111. |
in the present study we investigated six mrt cell lines (tm87-16, stm91-01, ttc642, ttc549, yam-rtk-1 and ttc1240), for cd133, nestin and musashi-1 (msi-1), which are considered to be csc as well as neural stem cell (nsc) markers, using assays for cell viability and apoptosis, reverse transcriptional polymerase chain reaction (rt-pcr), semi-quantitative pcr and western blot analysis before and after differentiation-induction with n-(4-hydroxyphenyl) retinamid (4-hpr). |
2010-03-30 |
2023-08-12 |
Not clear |
| A-Yong Cao, Wei Jin, Peng-Cheng Shi, Gen-hong Di, Zhen-Zhou Shen, Zhi-Ming Sha. Identification and characterization of two novel germ line p53 mutations in the non-LFS/non-LFL breast cancer families in Chinese population. Breast cancer research and treatment. vol 119. issue 2. 2010-03-10. PMID:19238535. |
and then we performed flow cytometry analysis in the transfected cells to determine the status of cell apoptosis, and real-time pcr as well as western blot analysis to ascertain the expression of p53, p21, and p27. |
2010-03-10 |
2023-08-12 |
Not clear |
| Li Wang, Xing Zheng, Hong-Lin Xiang, Xiao-Hua Fu, Jian-Guo Ca. 7-difluoromethyl-5,4'-dimethoxygenistein, a novel agent protecting against vascular endothelial injury caused by oxidative stress. Clinical and experimental pharmacology & physiology. vol 36. issue 12. 2010-02-25. PMID:19793105. |
human umbilical vein endothelial cells were treated with either genistein (10 micromol/l) or various concentrations of dfmgen (0.1, 0.3, 1, 3 and 10 micromol/l) for 30 min before exposure to 1 mmol/l h(2)o(2) for 24 h. the generation of reactive oxygen species (ros) was assessed by fluorescence flow cytometry, the release of lactate dehydrogenase (ldh) was examined by biochemical assay, cell viability was measured by the 3-(4,5-dimethyl-2 thiazoyl)-2,5-diphenyl-2h-tetrazolium bromide assay, cell apoptosis was detected by flow cytometry and the expression of caspase 3 was examined by western blot analysis. |
2010-02-25 |
2023-08-12 |
human |
| Sahdeo Prasad, Esha Madan, Nidhi Nigam, Preeti Roy, Jasmine George, Yogeshwer Shukl. Induction of apoptosis by lupeol in human epidermoid carcinoma A431 cells through regulation of mitochondrial, Akt/PKB and NFkappaB signaling pathways. Cancer biology & therapy. vol 8. issue 17. 2010-02-12. PMID:19625778. |
the rt-pcr and western blot analysis showed that lupeol-induced apoptosis was associated with caspase dependent mitochondrial cell death pathway through activation of bax, caspases, apaf1, decrease in bcl-2 expression and subsequent cleavage of parp. |
2010-02-12 |
2023-08-12 |
human |
| Maurice Schallenberg, Petar Charalambous, Solon Thano. GM-CSF regulates the ERK1/2 pathways and protects injured retinal ganglion cells from induced death. Experimental eye research. vol 89. issue 5. 2010-02-04. PMID:19560459. |
with respect to apoptosis, western blot analysis revealed a decreased bad-expression and an increased bcl-2-expression after co-incubation with gm-csf. |
2010-02-04 |
2023-08-12 |
human |
| Bian Li-juan, Liao Bing, Li Zhi, Li Yang, Liang Ying-ji. Hepatocyte growth factor suppresses tumor cell apoptosis in nasopharyngeal carcinoma by upregulating Bcl-2 protein expression. Pathology, research and practice. vol 205. issue 12. 2010-01-28. PMID:19625133. |
npc cells were treated with hgf (25 ng/ml), followed by an assay for cell viability and apoptosis, as well as by an expression analysis of bcl-2 and bax using immunostaining and western blot. |
2010-01-28 |
2023-08-12 |
Not clear |
| Guo-Guang Wang, Min Xu, Hai-Feng Duan, Gen-Bao Zhan. [Apoptosis of K562 cells induced by extract of Agkistrodon Halys' venom]. Zhongguo shi yan xue ye xue za zhi. vol 16. issue 3. 2010-01-12. PMID:18549620. |
the inhibition of k562 cell proliferation was measured by mtt assay; the morphologic changes of k562 cells was observed by microscopy; the apoptosis of k562 cells was measured by flow cytometry; the activity of extracellular signal-regulated kinase (erk) in k562 cells was detected by western blot. |
2010-01-12 |
2023-08-12 |
Not clear |
| Yi He, Jian-Min Yang, Jian-Min Wang, Hong Zhou, Shu-Qing Lü, Xiao-Xia H. [Synergistic effects of arsenic trioxide and proteasome inhibitor bortezomib on apoptosis induction in Raji cell line]. Zhongguo shi yan xue ye xue za zhi. vol 16. issue 4. 2010-01-12. PMID:18718063. |
the apoptosis related elements such as caspase-3, bcl-2, bax, jnk2 and ikappab-alpha, were measured with western blot. |
2010-01-12 |
2023-08-12 |
Not clear |
| Lian Yu Guo, Eun Ji Joo, Kun Ho Son, Su Jin Jeon, Sehyun Jang, Eun Myoung Shin, Hong Yu Zhou, Yeong Shik Ki. Cimiside E arrests cell cycle and induces cell apoptosis in gastric cancer cells. Archives of pharmacal research. vol 32. issue 10. 2010-01-04. PMID:19898801. |
the mechanism of apoptosis was further elucidated through western blot, rt-pcr, morphology, annexin v-fitc/pi staining and cell cycle analysis. |
2010-01-04 |
2023-08-12 |
Not clear |
| Claudia Hofmann, Elisabeth Lippert, Werner Falk, Jürgen Schölmerich, Gerhard Rogler, Florian Obermeie. Primary human colonic epithelial cells are transiently protected from anoikis by a Src-dependent mechanism. Biochemical and biophysical research communications. vol 390. issue 3. 2009-12-29. PMID:19852943. |
induction of apoptosis was assessed by caspase-3 activity assay; activation of survival pathways was analyzed by western blot. |
2009-12-29 |
2023-08-12 |
human |
| Y Deng, C Lin, J Zheng, X Liang, J Chen, M Fu, P Xiao, M W. Mechanisms of arsenic trioxide induced apoptosis of human cervical cancer HeLa cells and protection by Bcl-2. Science in China. Series C, Life sciences. vol 42. issue 6. 2009-12-16. PMID:18726487. |
rt-pcr, northern blot, western blot analysis revealed that as(2)o(3) induced hela cell apoptosis possibly via decreasing the expression of c-myc and viral genes. |
2009-12-16 |
2023-08-12 |
human |
| L Belkacemi, C H Chen, M G Ross, M Desa. Increased placental apoptosis in maternal food restricted gestations: role of the Fas pathway. Placenta. vol 30. issue 9. 2009-12-15. PMID:19616844. |
placenta apoptosis was quantified using terminal dutp nick-end labeling (tunel) assay and the data were compared to immunodetection of cleaved caspase-3, fas and fasl followed by western blot quantification of fas, fasl, caspase-8 and -3, tbid, and poly-adp-ribose polymerase (parp). |
2009-12-15 |
2023-08-12 |
Not clear |
| Hong-Fang Tao, Qun Hu, Jian-Lin Fang, Ai-Guo Liu, Shuang-You Liu, Liu-Qing Zhang, Ying H. [Effects of SODD and survivin on leukemia cell apoptosis induced by chemotherapeutic drugs]. Zhongguo shi yan xue ye xue za zhi. vol 15. issue 3. 2009-12-11. PMID:17605853. |
after jurkat cells were induced by chemotherapeutic drugs, the translocated phosphatidylserine was labeled with annexin v/pi, and the apoptosis incidence was measured by fcm; the expression changes of sodd, caspase 3, caspase 8 and caspase 9 were determined by western blot; the changes of survivin mrna and protein were determined by rt-pcr and immunohistochemistry sabc method respectively. |
2009-12-11 |
2023-08-12 |
human |
| Jun-Ling Liu, Rong-Xi Yu, Jun-Fa Che. [Apoptosis of HL-60 cells induced by artesunate in vitro]. Zhongguo shi yan xue ye xue za zhi. vol 15. issue 3. 2009-12-11. PMID:17605854. |
the inhibition of art on hl-60 cells were evaluated by means of mtt assay; cell apoptosis was detected by light microscopy, agarose gel electro-phoresis, flow cytometry; western blot was used to analyze the expression of bcl-2 and icad in cells during apoptosis induced by art. |
2009-12-11 |
2023-08-12 |
Not clear |
| Yoon Jung Choi, So Young Kim, Jung Min Oh, Yong Sung Juhn. Stimulatory heterotrimeric G protein augments gamma ray-induced apoptosis by up-regulation of Bak expression via CREB and AP-1 in H1299 human lung cancer cells. Experimental & molecular medicine. vol 41. issue 8. 2009-12-09. PMID:19381065. |
expression of constitutively active mutant of the alpha subunit of gs (galphasql) augmented gamma ray-induced apoptosis via mitochondrial dependent pathway when assessed by clonogenic assay, facs analysis of pi stained cells, and western blot analysis of the cytoplasmic translocation of cytochrome c and the cleavage of caspase-3 and ploy(adp-ribose) polymerase (parp) in h1299 human lung cancer cells. |
2009-12-09 |
2023-08-12 |
human |
| Biman B Mandal, S C Kund. Self-assembled silk sericin/poloxamer nanoparticles as nanocarriers of hydrophobic and hydrophilic drugs for targeted delivery. Nanotechnology. vol 20. issue 35. 2009-11-24. PMID:19671963. |
up-regulation of pro-apoptotic protein bax, down-regulation of anti-apoptotic protein bcl-2 and cleavage of regulatory protein parp through western blot analysis suggested further drug-induced apoptosis in cells. |
2009-11-24 |
2023-08-12 |
Not clear |
| Sun Ha Lee, Tae-Hyun Yoo, Bo-Young Nam, Dong Ki Kim, Jin Ji Li, Dong-Sub Jung, Seung-Jae Kwak, Dong-Ryeol Ryu, Seung Hyeok Han, Jung Eun Lee, Sung Jin Moon, Dae Suk Han, Shin-Wook Kan. Activation of local aldosterone system within podocytes is involved in apoptosis under diabetic conditions. American journal of physiology. Renal physiology. vol 297. issue 5. 2009-11-17. PMID:19710242. |
western blot for apoptosis-related molecules, hoechst 33342 staining, and terminal deoxynucleotidyl transferase-mediated dutp nick end labeling (tunel) assay were performed to determine apoptosis. |
2009-11-17 |
2023-08-12 |
rat |
| Zhang Xichu. Long-term exposure to various types of dietary fat modulates acrylamide-induced preneoplastic lesions of colon mucosa through Wnt/beta-catenin signaling in rats. Toxicology mechanisms and methods. vol 19. issue 4. 2009-11-17. PMID:19778218. |
at 48 weeks, normal appearing colon mucosal proliferation and apoptosis were evaluated by 5-bromo-2'-deoxyuridine (brdu) incorporation and percentages of fragmented dna, respectively; the expressions of beta-catenin, cyclin d1, wnt2, wnt3, and wnt5a of normal appearing colon mucosa were analyzed by western blot analysis. |
2009-11-17 |
2023-08-12 |
rat |