| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Guanbin Qi, Lei L. Long non-coding RNA PVT1 contributes to cell growth and metastasis in non-small-cell lung cancer by regulating miR-361-3p/SOX9 axis and activating Wnt/β-catenin signaling pathway. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie. vol 126. 2021-01-26. PMID:32197208. |
cell proliferation, apoptosis, migration and invasion were measured by 3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2-h-tetrazolium bromide (mtt), flow cytometry, transwell assays, severally. |
2021-01-26 |
2023-08-13 |
Not clear |
| Linxia Li, Kaihan Yang, Fang Ye, Yi Xu, Lili Cao, Jia Shen. Abnormal expression of TRIAP1 and its role in gestational diabetes mellitus-related pancreatic β cells. Experimental and therapeutic medicine. vol 21. issue 3. 2021-01-26. PMID:33488796. |
mtt and flow cytometry assays were performed to determine the viability and apoptosis of pancreatic β cells. |
2021-01-26 |
2023-08-13 |
human |
| R C Zou, Z T Guo, D Wei, Z T Shi, Z C Ye, G Zhai, C Zhong, B Tang, L Wang, J Y G. Downregulation of CDCA3 expression inhibits tumor formation in pancreatic cancer. Neoplasma. vol 67. issue 6. 2021-01-25. PMID:32701354. |
the effects of cdca3 downregulation on cell proliferation, apoptosis, and colony information were investigated through mtt assay, annexin v-apc single staining cell apoptosis detection, and colony formation test. |
2021-01-25 |
2023-08-13 |
human |
| Xueyan Zhang, Wen Liu, Shuxiang We. Luteolin affects keloid fibroblast proliferation and apoptosis by regulating FRAT1 gene expression. Cellular and molecular biology (Noisy-le-Grand, France). vol 66. issue 3. 2021-01-22. PMID:32538769. |
mtt method, western blot, flow cytometry, and real-time quantitative pcr (qpcr) were used to detect cell proliferation, cyclin d1 (cyclind1), p21, b-cell lymphoma / leukemia-2 (bcl-2), bcl-2 related x protein (bax), frat1 protein expression, apoptosis and arhi mrna expression. |
2021-01-22 |
2023-08-13 |
Not clear |
| Yanbo Yue, Lili He, Mi Tian, Xin L. Construction of SIRT1 gene shRNA lentivirus vector and its effect on the proliferation of breast cancer cells. Cellular and molecular biology (Noisy-le-Grand, France). vol 66. issue 3. 2021-01-22. PMID:32538772. |
mtt assay was used to detect cell activity, transwell cell was used to detect cell invasion and migration, and cell apoptosis detected by flow cytometry. |
2021-01-22 |
2023-08-13 |
human |
| Dayin Chen, Tingyu Chen, Yingxue Guo, Chennan Wang, Longxin Dong, Chunfeng L. Suppressive effect of platycodin D on bladder cancer through microRNA-129-5p-mediated PABPC1/PI3K/AKT axis inactivation. Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas. vol 54. issue 3. 2021-01-22. PMID:33470388. |
cell viability and growth were evaluated using mtt, edu, and colony formation assays, and cell apoptosis was determined using hoechst 33342 staining and flow cytometry. |
2021-01-22 |
2023-08-13 |
human |
| Yingchun Xu, Chunbo Miao, Jinzhen Cui, Xiaoli Bia. miR-92a-3p promotes ox-LDL induced-apoptosis in HUVECs via targeting SIRT6 and activating MAPK signaling pathway. Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas. vol 54. issue 3. 2021-01-22. PMID:33470395. |
flow cytometry assay and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2h-tetrazol-3-ium bromide (mtt) assay were performed to evaluate effects of oxidized low-density lipoprotein (ox-ldl) on proliferation and apoptosis of human umbilical vein endothelial cells (huvecs), respectively. |
2021-01-22 |
2023-08-13 |
human |
| Weiqun Lu, Zhiliang Huang, Jia Wang, Haiying Li. Long non-coding RNA DANCR accelerates colorectal cancer progression via regulating the miR-185-5p/HMGA2 axis. Journal of biochemistry. 2021-01-22. PMID:33481014. |
both gain-of-function and loss-of-function cell models for dancr were established, and then mtt, wound healing and transwell, flow cytometry assays were carried out to detect the proliferation, migration, invasion, cell cycle and apoptosis of crc cells. |
2021-01-22 |
2023-08-13 |
Not clear |
| Hailong Tang, Li Xu, Xi Cen, Li Yang, Juan Feng, Guang Li, Huafeng Zhu, Shan Gao, Yan Yu, Yaping Zhao, Zhiqiang Tian, Liping Hou, Shuchun Yu, Guangxun Ga. CDK5 inhibition in vitro and in vivo induces cell death in myeloma and overcomes the obstacle of bortezomib resistance. International journal of molecular medicine. vol 45. issue 6. 2021-01-21. PMID:32236619. |
the aim of this study is to investigate the effects of cdk5 on the viability of mm cells and bortezomib resistance using western blotting, immunohistochemistry, transient transfection, mtt assays, cell cycle analysis, apoptosis assays and a myeloma xenograft mouse model. |
2021-01-21 |
2023-08-13 |
mouse |
| Zheng Chen, Miaoxing Wu, Jiahua Li. Kelch-like protein 14 promotes proliferation and migration of ovarian cancer cells. International journal of clinical and experimental pathology. vol 13. issue 12. 2021-01-12. PMID:33425096. |
after constructing cell lines with a knocked down klhl14 gene, we used the mtt assay, flow cytometry with propidium iodide (pi), annexin v-fitc/pi, and transwell assay and found that knockdown of klhl14 gene inhibited proliferation of a-2780 cells, caused cell g0/g1 phase arrest, promoted apoptosis, and inhibited migration and invasiveness. |
2021-01-12 |
2023-08-13 |
Not clear |
| S A Pérez, M G Montalbán, G Carissimi, P Licence, G Víllor. In vitro cytotoxicity assessment of monocationic and dicationic pyridinium-based ionic liquids on HeLa, MCF-7, BGM and EA.hy926 cell lines. Journal of hazardous materials. vol 385. 2021-01-11. PMID:31727529. |
the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (mtt) assay, cell cycle arrest assays, apoptosis experiments and orange staining were carried out. |
2021-01-11 |
2023-08-13 |
Not clear |
| Fang Wang, Jiarui Li, Xinyue Tang, Kexin Huang, Lan Che. Polyelectrolyte three layer nanoparticles of chitosan/dextran sulfate/chitosan for dual drug delivery. Colloids and surfaces. B, Biointerfaces. vol 190. 2021-01-11. PMID:32155455. |
subsequently the cytotoxicity of nanoparticles was evaluated against hepg2 cells using mtt and apoptosis assay, resulting in synergistic effects between dual drugs and enhanced inhibition to cancer cells. |
2021-01-11 |
2023-08-13 |
Not clear |
| Caigu He, Xuehua Zheng, Xiuhong Lin, Xinying Chen, Chenyi She. Yunvjian-Medicated Serum Protects INS-1 Cells against Glucolipotoxicity-Induced Apoptosis through Autophagic Flux Modulation. Evidence-based complementary and alternative medicine : eCAM. vol 2020. 2021-01-11. PMID:33414841. |
mtt assay and flow cytometry were used to detect proliferation and apoptosis of ins-1 cells, respectively. |
2021-01-11 |
2023-08-13 |
Not clear |
| Ying-Juan Zheng, Tian-Song Liang, Juan Wang, Jing-Yi Zhao, Dao-Ke Yang, Zhang-Suo Li. Silencing lncRNA LOC101928963 Inhibits Proliferation and Promotes Apoptosis in Spinal Cord Glioma Cells by Binding to PMAIP1. Molecular therapy. Nucleic acids. vol 18. 2021-01-10. PMID:31670198. |
glioma cell proliferation and apoptosis were assessed by mtt assay and flow cytometry. |
2021-01-10 |
2023-08-13 |
Not clear |
| Zihao Chen, Hongping Ju, Ting Zhao, Shan Yu, Ping Li, Jing Jia, Nan Li, Xiaojie Jing, Bibo Tan, Yong L. hsa_circ_0092306 Targeting miR-197-3p Promotes Gastric Cancer Development by Regulating PRKCB in MKN-45 Cells. Molecular therapy. Nucleic acids. vol 18. 2021-01-10. PMID:31689616. |
annexin v-pi apoptosis flow cytometry, 3-(4,5dimethylthiazol- yl)-2,5dimethylthiazol-2-yl)-2,5diphenyltetrazolium bromide (mtt), wound healing, and transwell assays were applied to detect cell apoptosis, viability, migration, and invasion in mkn-45 cells, respectively. |
2021-01-10 |
2023-08-13 |
Not clear |
| Zhiqiang Li, Yaping Zhang, Nan Ding, Yudong Zhao, Zankai Ye, Lei Shen, Hanlu Yi, Yaobin Zh. Inhibition of lncRNA XIST Improves Myocardial I/R Injury by Targeting miR-133a through Inhibition of Autophagy and Regulation of SOCS2. Molecular therapy. Nucleic acids. vol 18. 2021-01-10. PMID:31734557. |
cell apoptosis was determined by flow cytometry analysis, and cell viability was used for 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-h-tetrazolium bromide, thiazolyl blue tetrazolium bromide (mtt) assay. |
2021-01-10 |
2023-08-13 |
mouse |
| Cheng Feng, Hai-Lin Zhang, Ang Zeng, Ming Bai, Xiao-Jun Wan. Tumor-Suppressive MicroRNA-216b Binds to TPX2, Activating the p53 Signaling in Human Cutaneous Squamous Cell Carcinoma. Molecular therapy. Nucleic acids. vol 20. 2021-01-10. PMID:32169806. |
a431 cells were transfected with mir-216b mimic, mir-216b inhibitor, or short interfering rna against tpx2 to evaluate cell proliferation, invasion, migration, and apoptosis using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (mtt) assay, scratch test, transwell assay, and flow cytometry. |
2021-01-10 |
2023-08-13 |
human |
| Wenyun Ting, Cheng Feng, Mingzi Zhang, Fei Long, Ming Ba. Overexpression of microRNA-203 Suppresses Proliferation, Invasion, and Migration while Accelerating Apoptosis of CSCC Cell Line SCL-1. Molecular therapy. Nucleic acids. vol 21. 2021-01-10. PMID:32668390. |
by conducting 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (mtt), scratch test, and transwell and flow cytometric analyses, mir-203 was witnessed to restrain scl-1 cell proliferation, migration, and invasion while accelerating their apoptosis. |
2021-01-10 |
2023-08-13 |
Not clear |
| Judong Luo, Hong Zhu, Hua Jiang, Yayun Cui, Mengjie Wang, Xinye Ni, Changsheng M. The effects of aberrant expression of LncRNA DGCR5/miR-873-5p/TUSC3 in lung cancer cell progression. Cancer medicine. 2021-01-09. PMID:29790668. |
for in vitro experiments, cell growth, migration, and invasion were significantly lower in a549 cells transfected with pcdna3.1-dgcr5 than pcdna3.1, which were verified by 5-diphenyltetrazolium bromide (mtt) assay, scratch test, and transwell assay, respectively, with no significant induction on cell apoptosis that was demonstrated by flow cytometry (fcm) assay. |
2021-01-09 |
2023-08-13 |
Not clear |
| Shulei Gong, Yu Li, Ling Lv, Wanfu Me. Restored microRNA-519a enhances the radiosensitivity of non-small cell lung cancer via suppressing EphA2. Gene therapy. 2021-01-08. PMID:33414521. |
mtt assay was used to measure cell proliferation, flow cytometry was performed for determination of cell cycle distribution and apoptosis. |
2021-01-08 |
2023-08-13 |
Not clear |