All Relations between apoptosis and kit

Publication Sentence Publish Date Extraction Date Species
Zhaoxia Yu, Aisa Rayile, Xiangyang Zhang, Ying Li, Qiang Zha. Ulinastatin protects against lipopolysaccharide-induced cardiac microvascular endothelial cell dysfunction via downregulation of lncRNA MALAT1 and EZH2 in sepsis. International journal of molecular medicine. vol 39. issue 5. 2017-06-29. PMID:28405676. then, cell permeability was evaluated by transendothelial electrical resistance, reactive oxygen species (ros) levels were assessed by 2,7-dichlorofluorescein diacetate, cell apoptosis was detected using annexin v-fitc apoptosis detection kit, and the expression levels of bcl-2, bax, caspase-3, metastasis-associated lung adenocarcinoma transcript 1 (malat1) and ezh2 were detected by rt-qpcr and/or western blotting. 2017-06-29 2023-08-13 rat
B Tokarz-Deptuła, B Hukowska-Szematowicz, P Niedźwiedzka-Rystwej, A Trzeciak-Ryczek, W Deptuł. Values of apoptosis of lymphocytes and granulocytes in peripheral blood of Polish mixed-breed rabbits in the annual cycle. Polish journal of veterinary sciences. vol 20. issue 1. 2017-06-29. PMID:28525323. assessment of apoptosis of lymphocytes and granulocytes was performed using as facscan cytometer by becton dickinson with facsdiva software (usa), using as apofluor® green caspase reagent kit (mp biomedicals, usa) to detect the activity of the total caspase pool 1, 3, 4, 5, 6, 7, 8, 9 in granulocytes and lymphocytes of rabbit peripheral blood. 2017-06-29 2023-08-13 rabbit
Oluseyi Ogunleye, Bertha Campo, Diana Herrera, Emiel D Post Uiterweer, Kirk P Conra. Relaxin confers cytotrophoblast protection from hypoxia-reoxygenation injury through the phosphatidylinositol 3-kinase-Akt/protein kinase B cell survival pathway. American journal of physiology. Regulatory, integrative and comparative physiology. vol 312. issue 4. 2017-06-27. PMID:28122716. the cells were treated with recombinant human relaxin or vehicle and apoptosis and/or necrosis evaluated by terminal deoxynucleotidyl transferase-mediated dutp nick end labeling (tunel), cellevent caspase-3/7 and sytox aadvanced kit, and propidium iodide staining as determined by fluorescence microscopy or flow cytometry. 2017-06-27 2023-08-13 human
Magdalena Włodarczyk, Jolanta Zdybel, Marek Próchniak, Zbigniew Osiński, Jacek Karamon, Teresa Kłapeć, Tomasz Cence. Viability assessment of Ascaris suum eggs stained with fluorescent dyes using digital colorimetric analysis. Experimental parasitology. vol 178. 2017-06-22. PMID:28479276. the best parameters were observed using the annexin v fitc apoptosis detection kit (dasi = 41 and 67). 2017-06-22 2023-08-13 Not clear
Bin He, Haiying Tao, Ailin Wei, Shiqing Liu, Xiaohai Li, Ren Che. Protection of carboxymethylated chitosan on chondrocytes from nitric oxide-induced apoptosis by regulating phosphatidylinositol 3-kinase/Akt signaling pathway. Biochemical and biophysical research communications. vol 479. issue 2. 2017-06-06. PMID:27644875. the cell viability was determined by cell counting kit (cck-8), cell apoptosis was detected by annexin-v/pi double staining assay kit. 2017-06-06 2023-08-13 rat
D Lin, X Jing, Y Chen, Y Liang, M Lei, S Peng, T Zhou, D Zheng, Z Zeng, X Wu, L Yang, S Xiao, J Liu, E Ta. Rifampicin pre-treatment inhibits the toxicity of rotenone-induced PC12 cells by enhancing sumoylation modification of α-synuclein. Biochemical and biophysical research communications. vol 485. issue 1. 2017-06-06. PMID:28132806. the cell viability and apoptosis rate were measured by a cck-8 assay kit and flow cytometry, respectively. 2017-06-06 2023-08-13 Not clear
Ling Liu, Ping Zhan, Dan Nie, Lingye Fan, Hairui Lin, Lanyang Gao, Xiguang Ma. Intermediate-Conductance-Ca2-Activated K Channel IKCa1 Is Upregulated and Promotes Cell Proliferation in Cervical Cancer Medical science monitor basic research. vol 23. 2017-06-01. PMID:28280257. cell apoptosis was assessed using the annexin v-fitc/propidium iodide (pi) double-staining apoptosis detection kit. 2017-06-01 2023-08-13 human
Zhong-Jun Du, Guan-Qun Cui, Juan Zhang, Xiao-Mei Liu, Zhi-Hu Zhang, Qiang Jia, Jack C Ng, Cheng Peng, Cun-Xiang Bo, Hua Sha. Inhibition of gap junction intercellular communication is involved in silica nanoparticles-induced H9c2 cardiomyocytes apoptosis via the mitochondrial pathway. International journal of nanomedicine. vol 12. 2017-05-22. PMID:28356735. the aim of the present study is to determine whether snps-decreased gjic promotes apoptosis in rat cardiomyocytes cell line (h9c2 cells) via the mitochondrial pathway using cck-8 kit, scrape-loading dye transfer technique, annexin v/pi double-staining assays, and western blot analysis. 2017-05-22 2023-08-13 rat
Li Qiao, Chengshan Xu, Qiang Li, Zhusong Mei, Xinji Li, Hong Cai, Wei Li. Photodynamic therapy activated STAT3 associated pathways: Targeting intrinsic apoptotic pathways to increase PDT efficacy in human squamous carcinoma cells. Photodiagnosis and photodynamic therapy. vol 14. 2017-05-17. PMID:26607555. apoptosis and cell viability after pdt were evaluated using annexin v-fitc apoptosis detection kit and mtt assay. 2017-05-17 2023-08-13 human
Muming Yu, Bin Lu, Yancun Liu, Ying Me, Lijun Wang, Hui L. Interference with Tim-3 protein expression attenuates the invasion of clear cell renal cell carcinoma and aggravates anoikis. Molecular medicine reports. vol 15. issue 3. 2017-05-09. PMID:28112366. apoptosis was measured using flow cytometry, e‑cadherin and n‑cadherin protein expression were determined using western blotting and a chemicon cell invasion assay kit was used to quantify the invasive capacity of 786‑o and caki‑2 cells. 2017-05-09 2023-08-13 Not clear
Jin-Guo Li, Qi Hao, Ying-Xue Liu, Peng Li, Peng Li, Xia-Yan Shao, Hua Tian, Yong-Qi Fang, Shu-Tong Ya. [Advanced glycated albumin induces macrophage apoptosis via activating caspase-12 pathway]. Sheng li xue bao : [Acta physiologica Sinica]. vol 68. issue 6. 2017-05-05. PMID:28004067. after incubation for 24 h, the cell viability and apoptosis were determined by using mtt assay and tunel detection kit, respectively. 2017-05-05 2023-08-13 Not clear
Bingqian Xie, Zhijian Xu, Liangning Hu, Gege Chen, Rong Wei, Guang Yang, Bo Li, Gaomei Chang, Xi Sun, Huiqun Wu, Yong Zhang, Bojie Dai, Yi Tao, Jumei Shi, Weiliang Zh. Pterostilbene Inhibits Human Multiple Myeloma Cells via ERK1/2 and JNK Pathway In Vitro and In Vivo. International journal of molecular sciences. vol 17. issue 11. 2017-04-28. PMID:27869675. in the present study, we examined the anti-tumor effect of pte on mm cell lines both in vitro and in vivo using the cell counting kit (cck)-8, apoptosis assays, cell cycle analysis, reactive oxygen species (ros) generation, jc-1 mitochondrial membrane potential assay, western blotting and tumor xenograft models. 2017-04-28 2023-08-13 mouse
Peijun Tian, Li Ya. Inhibition of MicroRNA-149-5p Induces Apoptosis of Acute Myeloid Leukemia Cell Line THP-1 by Targeting Fas Ligand (FASLG). Medical science monitor : international medical journal of experimental and clinical research. vol 22. 2017-04-28. PMID:28013316. aml cell line thp-1 was transfected with mir-149-5p mimic or inhibitor, and then cell apoptosis was determined using the apo percentage assay kit. 2017-04-28 2023-08-13 Not clear
Lian Jian, Yuan Lu, Shan Lu, Chengzhi L. Chemical Chaperone 4-Phenylbutyric Acid Reduces Cardiac Ischemia/Reperfusion Injury by Alleviating Endoplasmic Reticulum Stress and Oxidative Stress. Medical science monitor : international medical journal of experimental and clinical research. vol 22. 2017-04-24. PMID:28036323. a tunel apoptosis assay kit was used to detect apoptosis. 2017-04-24 2023-08-13 rat
Tie-Jun Liang, Zhong-Mei Zhou, Ying-Qing Cao, Ming-Ze Ma, Xiao-Jun Wang, Kai Jin. Gemcitabine-based polymer-drug conjugate for enhanced anticancer effect in colon cancer. International journal of pharmaceutics. vol 513. issue 1-2. 2017-04-21. PMID:27613255. gem-pl formulation exhibited a significantly higher apoptosis of cancer cells (∼25%) when stained using annexin-v/pi kit. 2017-04-21 2023-08-13 Not clear
Guicheng Li, Tao Li, Yunfeng Li, Shumin Cai, Zhiming Zhang, Zhenhua Zeng, Xingmin Wang, Youguang Gao, Yunfeng Li, Zhongqing Che. Ulinastatin inhibits oxidant-induced endothelial hyperpermeability and apoptotic signaling. International journal of clinical and experimental pathology. vol 7. issue 11. 2017-04-13. PMID:25550770. monolayer permeability was determined by transendothelial electrical resistance (ter); mitochondrial release of cytochrome c was determined by enzyme-linked immunosorbent assay; caspase-3 activity was measured by fluorometric assay; adherens junction protein β-catenin was detected by immunofluorescense staining; ratio of cell apoptosis was evaluated by annexin-v/pi double stain assay; mitochondrial membrane potential (Δψm) was determined with jc-1; intracellular atp content was assayed by a commercial kit; bax and bcl-2 expression were estimated by western blotting; intracellular reactive oxygen species (ros) level was measured by dcfh-da. 2017-04-13 2023-08-13 Not clear
K Wasilewska, Ł Zasiadczyk, L Fraser, M Mogielnicka-Brzozowska, W Korda. The benefits of cooling boar semen in long-term extenders prior to cryopreservation on sperm quality characteristics. Reproduction in domestic animals = Zuchthygiene. vol 51. issue 5. 2017-04-10. PMID:27554400. the vybrant apoptosis assay kit was used to analyse the proportions of viable and plasma membrane apoptotic-like changes in spermatozoa. 2017-04-10 2023-08-13 Not clear
Chang-Long Xu, Bo Zheng, Ji-Hua Pei, Su-Jian Shen, Jian-Zhang Wan. Embelin induces apoptosis of human gastric carcinoma through inhibition of p38 MAPK and NF-κB signaling pathways. Molecular medicine reports. vol 14. issue 1. 2017-04-06. PMID:27175982. cellular and nuclear apoptosis were analyzed with an annexin v‑fitc/pi apoptosis detection kit and dapi staining assay, respectively. 2017-04-06 2023-08-13 human
Hongyan Zhao, Dali Zhao, Huilin Jin, Hongwei Li, Xiaoying Yang, Liwei Zhuang, Tiefu Li. Bufalin reverses intrinsic and acquired drug resistance to cisplatin through the AKT signaling pathway in gastric cancer cells. Molecular medicine reports. vol 14. issue 2. 2017-04-06. PMID:27357249. effects of cisplatin and bufalin on gc cells were measured by a cell counting kit, apoptosis was analyzed by flow cytometry, and immunoblotting was used to detect proteins associated with the akt signaling pathway. 2017-04-06 2023-08-13 human
Xin-Jun Chen, Mian-Yun Wu, Deng-Hui Li, Jin Yo. Apigenin inhibits glioma cell growth through promoting microRNA-16 and suppression of BCL-2 and nuclear factor-κB/MMP‑9. Molecular medicine reports. vol 14. issue 3. 2017-04-06. PMID:27430517. cell counting kit‑8 solution and annexin v-fluorescein isothiocyanate/propidium iodide apoptosis detection kit were used to analyze the effect of apigenin on u87 cell viability and apoptotic cell death. 2017-04-06 2023-08-13 human