| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Lei Wang, Weijun Yan, Jing Wan. Silencing MEKK3 attenuates cardiomyocyte injury caused by hypoxia/reoxygenation via the sonic hedgehog pathway. Journal of cellular physiology. 2019-11-20. PMID:30712259. |
cell viability, cell apoptosis, caspase 3/7 activity, and cleaved-caspase 3 expression were tested using a cck-8 assay, cell death detection plus elisa, caspase-glo 3/7 assay kit and western blot analysis, respectively. |
2019-11-20 |
2023-08-13 |
Not clear |
| Anjun Zuo, Peng Zhao, Yu Zheng, Hui Hua, Xingang Wan. Tripterine inhibits proliferation, migration and invasion of breast cancer MDA-MB-231 cells by up-regulating microRNA-15a. Biological chemistry. 2019-11-20. PMID:30913029. |
viability, proliferation, migration, invasion and apoptosis of mda-mb-231 cells were assessed using the cell counting kit-8 (cck-8) assay, brdu incorporation assay, annexin v-fitc/pi apoptosis detection kit and two-chamber transwell assay, respectively. |
2019-11-20 |
2023-08-13 |
Not clear |
| Shanshan Wang, Mingyue Zhu, Qiaoyun Wang, Yuli Hou, Lei Li, Honglei Weng, Yan Zhao, Dexi Chen, Huiguo Ding, Junli Guo, Mengsen L. Alpha-fetoprotein inhibits autophagy to promote malignant behaviour in hepatocellular carcinoma cells by activating PI3K/AKT/mTOR signalling. Cell death & disease. vol 9. issue 10. 2019-11-13. PMID:30301886. |
cell autophagy, apoptosis, proliferation, migration and invasion were analysed with western blotting, co-immunoprecipitation (coip), immunofluorescence, animal models, mtt assays, flow cytometry (fcm), cell counting kit (cck)-8, and scratch and transwell assays. |
2019-11-13 |
2023-08-13 |
Not clear |
| Xiguang Yang, Jingliang He, Bowei Li, Jinqian Zhang, Ke He, Xiaopeng Duan, Rui Huang, Guoan Xian. SWNHs (Single-Wall Carbon Nanohorns) Supervises Endoplasmic Reticulum (ER) Stress in Hepatocellular Carcinoma. Journal of nanoscience and nanotechnology. vol 18. issue 10. 2019-11-11. PMID:29954489. |
and then 38 apoptosis proteins were determined using human apoptosis antibody array kit. |
2019-11-11 |
2023-08-13 |
mouse |
| Fang Yuan, Lu Liu, Yonghong Lei, Yi H. MiRNA-142-3p increases radiosensitivity in human umbilical cord blood mononuclear cells by inhibiting the expression of CD133. Scientific reports. vol 8. issue 1. 2019-10-22. PMID:29618746. |
cck-8 kit was used to detect cell viability, annexin v-fitc/pi cell apoptosis detection kit was used for the detection of apoptotic cells and the brdu assay was performed by flow cytometry. |
2019-10-22 |
2023-08-13 |
human |
| Ding-Yan Lu, Jing Li, Jia Sun, Wei-Na Xue, Bin He, Yong-Jun Li, Yong-Lin Wang, Chang-Hu Lin, Ting Li. [Shenxiong Glucose Injection inhibits H_2O_2-induced apoptosis of H9c2 cells by activating PI3K/AKT pathway]. Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica. vol 44. issue 17. 2019-10-21. PMID:31602952. |
cell viability was detected by mts assay,morphological changes of apoptosis were observed by ao/eb fluorescence staining,apoptosis rate was detected by annexin/pi method,cell expression profile was detected by gene chip technology,the mrna of pik3 ca,bcl-2,bax,caspase-3 and gapdh were detected by qrt-pcr,the protein expression levels of pik3 ca,akt,p-akt,bcl-2,bax and caspase-3 were detected by western blot,and the contents of ldh and mda were detected by kit. |
2019-10-21 |
2023-08-13 |
Not clear |
| Yunjie Zhou, Huichao Zhang, Zhixia Su. [Effect of miR-873 on cardiomyocyte apoptosis induced by hypoxia reoxygenation and its mechanism]. Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences. vol 44. issue 8. 2019-10-17. PMID:31570671. |
cell apoptosis elisa kit and cysteine-containing, aspartate-specific proteases-3 (caspase-3) activity kit was used to detect cell apoptosis and caspase-3 activity, respectively. |
2019-10-17 |
2023-08-13 |
mouse |
| Wen-Hu Liu, Jiang-Bei Yuan, Fan Zhang, Jin-Xia Chan. [Curcumin inhibits proliferation,migration and invasion of gastric cancer cells via Wnt3a/β-catenin/EMT signaling pathway]. Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica. vol 44. issue 14. 2019-10-16. PMID:31602860. |
the migration and invasiveness of cells were determined by wound healing and transwell assays,the apoptosis rate was assessed by flow cytometry,the expression of n-cadherin,e-cadherin,snail1,wnt3 a,p-β-catenin,p-lrp6,bcl-2 and bax were detected by western blot,and the enzymatic activity of caspase-3,caspase-8 and caspase-9 was evaluated via caspase kit. |
2019-10-16 |
2023-08-13 |
human |
| Tao Yao, Dongqing Zha, Ping Gao, Hua Shui, Xiaoyan W. MiR-874 alleviates renal injury and inflammatory response in diabetic nephropathy through targeting toll-like receptor-4. Journal of cellular physiology. vol 234. issue 1. 2019-10-02. PMID:30171701. |
the cell counting kit (cck-8) assay and 5-ethynyl-2'-deoxyuridine (edu) assay demonstrated that glucose simulation significantly inhibited podocyte proliferation and induced cell apoptosis, which can be reversed by mir-874 mimics significantly. |
2019-10-02 |
2023-08-13 |
mouse |
| Jiping Zhu, Jingjing Li, Xiaomei Wang, Xiaoting Feng, Yi L. Nonstructural Protein NP1 of Human Bocavirus 1 suppresses the growth of A549 cell by promoting autophagy. The new microbiologica. vol 42. issue 3. 2019-09-20. PMID:31157401. |
the apoptosis of a549 was evaluated by apoptosis detection kit and expression of caspase3. |
2019-09-20 |
2023-08-13 |
human |
| Shan Yang, Peng Wu, Jianwen Xiao, Li Jian. Overexpression of COX6B1 protects against I/R‑induced neuronal injury in rat hippocampal neurons. Molecular medicine reports. vol 19. issue 6. 2019-09-09. PMID:31059068. |
cell viability was assessed using a cell counting kit, and the levels of apoptosis and cytosolic ca2+ were analyzed by flow cytometry. |
2019-09-09 |
2023-08-13 |
rat |
| Hamid Bakhshi Aliabad, Soudeh Khanamani Falahati-Pour, Hadis Ahmadirad, Maryam Mohamadi, Mohammad Reza Hajizadeh, Mehdi Mahmood. Vanadium complex: an appropriate candidate for killing hepatocellular carcinoma cancerous cells. Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine. vol 31. issue 6. 2019-09-03. PMID:30255365. |
the early apoptosis and necrosis/late apoptosis were determined by means of annexin v/pi apoptosis detection kit. |
2019-09-03 |
2023-08-13 |
human |
| Hongbo Li, Yanan Liu, Minyong Wen, Fu Zhao, Zhihui Zhao, Yunsong Liu, Xinfeng Lin, Lin Wan. Hydroxysafflor yellow A (HSYA) alleviates apoptosis and autophagy of neural stem cells induced by heat stress via p38 MAPK/MK2/Hsp27-78 signaling pathway. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie. vol 114. 2019-08-29. PMID:30954890. |
annexin v/pi apoptosis kit was used to detect nsc apoptosis. |
2019-08-29 |
2023-08-13 |
rat |
| Yang Shu, Junpeng Long, Weixiong Guo, Wei Y. MicroRNA‑195‑5p inhibitor prevents the development of osteoarthritis by targeting REGγ. Molecular medicine reports. vol 19. issue 6. 2019-08-26. PMID:30942447. |
then an mtt assay was conducted to detect cell proliferation ability, and an annexin v‑fluorescein isothiocyanate/propidium iodide apoptosis detection kit was used to measure cell apoptosis. |
2019-08-26 |
2023-08-13 |
Not clear |
| Qin Xia, Jiangdong Ni, Jun Huang, Baiqi Pan, Mingming Yan, Wenzhao L. [Suppression of miR-30a/HMGA2-mediated autophagy in osteosarcoma cells impacts chemotherapeutics-induced apoptosis]. Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences. vol 44. issue 7. 2019-08-23. PMID:31413213. |
the expression levels of beclin 1, lc3b and p62 in osteosarcoma u2-os cells after treatment of cisplatin and doxorubicin in these 3 groups were detected by western blotting; the level of autophagy was detected by monodansylcada (mdc) staining; the level of ros was detected by dihydroethidium (dhe); the level of cell surviving rate was detected by cell counting kit-8 (cck-8); the level of apoptosis was detected by annexin apc/pi double staining; the level of mitochondria oxidative damage was detected by mitochondrial membrane potential assay kit with jc-1 (jc-1 method). |
2019-08-23 |
2023-08-13 |
Not clear |
| Qianying Liu, Zhixin Lei, Kaixiang Zhou, Huiru Yu, Shenhe Liu, Qiliang Sun, Xu Wang, Menghong Dai, Zonghui Yua. N-O Reduction and ROS-Mediated AKT/FOXO1 and AKT/P53 Pathways Are Involved in Growth Promotion and Cytotoxicity of Cyadox. Chemical research in toxicology. vol 31. issue 11. 2019-08-06. PMID:30265530. |
in the present study, cyadox and its main metabolites of cy1, cy4, cy6, and cy12 were selected; the growth promotion characteristic was indicated by the mrna level of egf; and the cytotoxicity of cyadox was determined by methylthiazol tetrazolium bromide (mtt) assay, lactate dehydrogenase (ldh) release, and annexin v-fitc/pi apoptosis detection kit with flow cytometry. |
2019-08-06 |
2023-08-13 |
Not clear |
| Zhenwei Deng, Huaiming Wang, Guohu Guo, Xiyao Li, Yongchang Cai, Yuxin Tang, Yijun Wang, Jiabao Li, Zhibin Lu, Xueqiao Yu, Ruiping Li, Libo L. Next-Generation Sequencing Analysis of mRNA Profile in Cisplatin-Resistant Gastric Cancer Cell Line SGC7901. Medical science monitor : international medical journal of experimental and clinical research. vol 25. 2019-07-19. PMID:30938333. |
the data were verified by quantitative polymerase chain reaction and their functional role was evaluated by cell counting kit 8 assay and cell apoptosis and cell cycle flow cytometric analysis. |
2019-07-19 |
2023-08-13 |
human |
| Yongjun Hu, Zhaofen Zheng, Hongwei Pan, Jianqiang Peng, Yangping Luo, Bo Cui, Xin Zhon. Notch1 signaling activation protected myocardium against hypoxia injury via reducing programmed cell death. Pakistan journal of pharmaceutical sciences. vol 30. issue 6(Supplementary). 2019-07-08. PMID:29188781. |
condition of hypoxic experiment was 1% oxygen centration and culturing for 12hours, then the cell proliferation activity and apoptosis rate was assessed by mts kit and flow cytometry, respectively. |
2019-07-08 |
2023-08-13 |
Not clear |
| Zihao Chen, Hongping Ju, Shan Yu, Ting Zhao, Xiaojie Jing, Ping Li, Jing Jia, Nan Li, Bibo Tan, Yong L. Prader-Willi region non-protein coding RNA 1 suppressed gastric cancer growth as a competing endogenous RNA of Clinical science (London, England : 1979). vol 132. issue 9. 2019-07-01. PMID:29535266. |
cell proliferation, apoptosis rate, metastasis abilities were respectively determined by cell counting kit 8 (cck8), flow cytometry, wound healing, and transwell assay. |
2019-07-01 |
2023-08-13 |
Not clear |
| Eva Serrano-Candelas, Erola Ainsua-Enrich, Arnau Navinés-Ferrer, Paulo Rodrigues, Alfonso García-Valverde, Sarah Bazzocco, Irati Macaya, Joaquín Arribas, César Serrano, Joan Sayós, Diego Arango, Margarita Marti. Silencing of adaptor protein SH3BP2 reduces KIT/PDGFRA receptors expression and impairs gastrointestinal stromal tumors growth. Molecular oncology. vol 12. issue 8. 2019-07-01. PMID:29885053. |
our current study shows that sh3bp2 is expressed in primary tumors and cell lines from gist patients and that sh3bp2 silencing leads to a downregulation of oncogenic kit and pdgfra expression and an increase in apoptosis in imatinib-sensitive and imatinib-resistant gist cells. |
2019-07-01 |
2023-08-13 |
human |