| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Zhifang Tang, Jingyuan Li, Chuan L. Post-Transcriptional Regulator RBM47 Stabilizes FBXO2 mRNA to Advance Osteoarthritis Development: WGCNA Analysis and Experimental Validation. Biochemical genetics. 2023-12-09. PMID:38070024. |
functional analysis results from the mtt assay, flow cytometry, evaluation of ldh activities and inflammatory mediators, and western blot analysis of extracellular matrix (ecm) proteins, showed that rbm47 knockdown significantly alleviated inflammation, apoptosis, and ecm degradation in interleukin 1β (il-1β)-treated chondrocytes. |
2023-12-09 |
2023-12-17 |
Not clear |
| Yiting Hou, Lihua Dong, Lihua Ca. Saxagliptin reduces the injury of Alzheimer's disease cell model by down-regulating the expression of miR-483-5p. Cellular and molecular biology (Noisy-le-Grand, France). vol 69. issue 12. 2023-12-08. PMID:38063096. |
then the levels of mda, sod and gsh-px in each group were detected by elisa method; cell apoptosis was detected by flow cytometry; the protein expression levels of bax and bcl-2 were detected by western blot; the expression level of mir-483-5p was detected by rt-qpcr. |
2023-12-08 |
2023-12-17 |
Not clear |
| Daomin Lu, Yuzheng Yang, Wukai Ma, Xueming Yao, Yi Ling, Ying Huang, Qiuyi Wang, Chengyu Xia, Lei Ho. Stigmasterol Depresses the Proliferation and Facilitates the Apoptosis of Fibroblast-Like Synoviocytes via the PI3K/AKT Signaling Pathway in Collagen-Induced Arthritis Rats. Alternative therapies in health and medicine. 2023-12-08. PMID:38064612. |
in addition, we investigated the mechanism of stigmasterol inhibiting fls proliferation and promoting its apoptosis by qpcr, western blot, and immunofluorescence assays. |
2023-12-08 |
2023-12-17 |
rat |
| Xixi Li, Zhipeng Huang, Hua Chao, Xunxun Li. The mechanism of Semen Persicae-Flos Carthami in treating hypertrophic scar: A study based on network pharmacological analysis and in vitro experiments. Chemical biology & drug design. 2023-12-06. PMID:38056934. |
besides, a modified explant culture method was applied to separate primary hypertrophic scar fibroblasts (hsfs); mtt assay to detect cell viability of hsfs after treatment by sp-fc for 24 h; annexin v-fitc/pi double staining combined with flow cytometry to test apoptosis; western blot to check the protein expression level of p53; and real-time fluorescence quantitative pcr to determine mrna level of p53. |
2023-12-06 |
2023-12-10 |
Not clear |
| Yun Chen, Bocheng Zhang, Cui Zhong, Yuqing Zhou, Lei Xue, Chenhui Luo, Liang Yi, Qian Gong, Ying Lon. let-7g sensitized liver cancer cells to 5-fluorouracil by downregulating ABCC10 expression. Chemical biology & drug design. 2023-12-06. PMID:38054583. |
the levels of biomarkers related to cell cycle progression and apoptosis were detected by western blot assays. |
2023-12-06 |
2023-12-10 |
mouse |
| Yunchang Cao, Hongbo Wen, Chaoqun Leng, Shaolong Fen. MiR-29a mediates the apoptotic effects of TNF-α on endothelial cells through inhibiting PI3K/AKT/BCL-2 axis. Journal of biochemical and molecular toxicology. 2023-12-04. PMID:38047396. |
real-time quantitative pcr and western blot were performed to detect the levels of target rnas and proteins/their phosphorylation in huvecs. tnf-α could inhibit huvec proliferation and induce huvec apoptosis in a positive dose- and time-dependent manner, with a similar way of mir-29a upregulation, but no effects on mir-29b/c. |
2023-12-04 |
2023-12-10 |
human |
| Qin Liu, Zhen-Peng Niu, Kun Yang, Jing-Rui Song, Xue-Nai Wei, Yu-Bing Huang, Chun-Mao Yuan, Yan-Mei L. Synergistic combination of isogarcinol isolated from edible fruits of Garcinia multiflora and dexamethasone to overcome leukemia glucocorticoid resistance. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie. vol 170. 2023-12-01. PMID:38039755. |
furthermore, western blot analysis revealed that iso and dex inhibits the pi3k/akt/mtor signaling pathway and promotes the nuclear translocation of glucocorticoid receptor (gr), which activates target genes nr3c1 and tsc22d3, leading to apoptosis in jurkat cells. |
2023-12-01 |
2023-12-10 |
mouse |
| Sungryeal Kim, Jeong Mi Kim, Eun Jeong Jeon, Ji Won Kim, Mi Eun Choi, Jin-Mi Park, Jeong-Seok Cho. Supernatant of activated platelet-rich plasma rejuvenated aging-induced hyposalivation in mouse. Scientific reports. vol 13. issue 1. 2023-12-01. PMID:38040732. |
the decrease of senescence, apoptosis, and inflammation observed by western blot in 22 m + saprp group. |
2023-12-01 |
2023-12-10 |
mouse |
| Tong Wu, Yanan Sheng, Yu Tian, Changyuan Wan. Vitexin Regulates Heat Shock Protein Expression by Modulating ROS Levels Thereby Protecting against Heat-Stress-Induced Apoptosis. Molecules (Basel, Switzerland). vol 28. issue 22. 2023-11-25. PMID:38005362. |
therefore, in this study, we used caco-2 cells to establish a heat stress model and vitamin c as a positive control to investigate the regulatory effects of vitexin on heat-stress-induced apoptosis and the related mechanisms using cell counting kit-8, flow cytometry, real-time quantitative polymerase chain reaction, and western blot. |
2023-11-25 |
2023-11-28 |
human |
| Yiping Zhu, Junling Ren, Xu Wu, Yuan Zhang, Ying Wang, Jinwen Xu, Qiuyu Tan, Yuxin Jiang, Yulei L. lncRNA ENST00000422059 promotes cell proliferation and inhibits cell apoptosis in breast cancer by regulating the miR-145-5p/KLF5 axis. Acta biochimica et biophysica Sinica. 2023-11-24. PMID:37997376. |
we further verify that mir-145-5p regulated klf5 expression to promote cell apoptosis and inhibit cell proliferation in bc via dual-luciferase reporter assay, western blot analysis, qrt-pcr, cck-8 assay and cell apoptosis assay. |
2023-11-24 |
2023-11-29 |
Not clear |
| Yiping Zhu, Junling Ren, Xu Wu, Yuan Zhang, Ying Wang, Jinwen Xu, Qiuyu Tan, Yuxin Jiang, Yulei L. lncRNA ENST00000422059 promotes cell proliferation and inhibits cell apoptosis in breast cancer by regulating the miR-145-5p/KLF5 axis. Acta biochimica et biophysica Sinica. 2023-11-24. PMID:37997376. |
subsequently, fish, western blot analysis, qrt-pcr, cck-8 and cell apoptosis assays verified that enst00000422059 increases klf5 protein expression by sponging mirna to promote cell proliferation and inhibit cell apoptosis. |
2023-11-24 |
2023-11-29 |
Not clear |
| Meng Yuan, Asli F Ceylan, Rifeng Gao, Hong Zhu, Yingmei Zhang, Jun Re. Selective inhibition of the NLRP3 inflammasome protects against acute ethanol-induced cardiotoxicity in an FBXL2-dependent manner. Acta biochimica et biophysica Sinica. 2023-11-23. PMID:37994158. |
levels of nlrp3 inflammasome, mitophagy and apoptosis are evaluated by western blot analysis and immunofluorescence techniques. |
2023-11-23 |
2023-11-29 |
mouse |
| Jian Hao, Zhenhan Li, Li Xie, Bingbing Yu, Boyuan Ma, Yubiao Yang, Xuchen Ma, Bitao Wang, Xianhu Zho. Syringaresinol promotes the recovery of spinal cord injury by inhibiting neuron apoptosis via activating the ubiquitination factor E4B/AKT Serine/Threonine kinase signal pathway. Brain research. 2023-11-22. PMID:37992795. |
second, mtt was employed to evaluate cell proliferation rate, western blot was performed to detect protein expression, rt-qpcr was conducted to detect mrna expression levels, flow cytometry and 5-ethynyl-2'-deoxyuridine (edu) staining were used to determine cell apoptosis, and immunofluorescence and immunohistochemistry were used to estimate the expression of rna binding fox-1 homolog 3 and clipped caspase 3. |
2023-11-22 |
2023-11-29 |
rat |
| Dong Li, Lianggang Li, Shiyong Dong, Yaqun Yu, Lin Zhang, Shengli Jian. ALKBH5 regulates N(6)-methyladenosine (m6A) methylation of MG53 to attenuate myocardial infarction by inhibiting apoptosis and oxidative stress. Journal of cardiovascular pharmacology. 2023-11-21. PMID:37989146. |
apoptosis was evaluated by tunel assay, flow cytometry, and western blot. |
2023-11-21 |
2023-11-29 |
mouse |
| Huifang Zhang, Yudan Zhang, Kaixuan Xu, Lawen Wang, Xin Zhou, Mingge Yang, Jiangli Xie, Hui L. Inhibition of FLT1 Attenuates Neurodevelopmental Abnormalities and Cognitive Impairment in Offspring Caused by Maternal Prenatal Stress. Applied biochemistry and biotechnology. 2023-11-18. PMID:37979086. |
the apoptosis level of hippocampal neurons and the expression of nmdars were detected by mtt assay, tunel assay, and western blot. |
2023-11-18 |
2023-11-20 |
rat |
| Shuta Tamura, Hiroshi Tazawa, Naoto Hori, Yuncheng Li, Motohiko Yamada, Satoru Kikuchi, Shinji Kuroda, Yasuo Urata, Shunsuke Kagawa, Toshiyoshi Fujiwar. p53-armed oncolytic adenovirus induces autophagy and apoptosis in KRAS and BRAF-mutant colorectal cancer cells. PloS one. vol 18. issue 11. 2023-11-16. PMID:37972012. |
western blot analysis demonstrated that obp-301 induced autophagy and that obp-702 induced autophagy and apoptosis in human crc cells. |
2023-11-16 |
2023-11-20 |
human |
| Si-Min Liang, Gui-Bin Liang, Hui-Ling Wang, Hong Jiang, Xian-Li Ma, Jian-Hua Wei, Ri-Zhen Huang, Ye Zhan. Discovery of 4-(N-dithiobenzyl piperazine)-1,8-naphthalimide as a potent multi-target antitumor agent with good efficacy, limited toxicity, and low resistance. European journal of medicinal chemistry. vol 263. 2023-11-16. PMID:37972528. |
results from the omics research, confocal immunofluorescence, western blot, transmission electron microscopy, and flow cytometry indicated that compound 5j exerted antitumor effects through multiple mechanisms, including ferroptosis, autophagy, apoptosis, and cell cycle arrest. |
2023-11-16 |
2023-11-20 |
Not clear |
| Zhujie Xu, Peng Wang, Zhenyu Wang, Hao Cui, Tianshu Gao, Zhenting Wang, Yi Li. ER-β accelerates the process of primary osteoporosis by promoting VEGFA-mediated apoptosis of osteoblasts. Genomics. 2023-11-15. PMID:37967683. |
and western blot results showed that apoptosis was most likely realized through the regulation of downstream apoptosis markers c-jun (c-jun n-terminal kinase, jnk) and gadd45g (growth arrest and dna damage-inducible protein 45 gamma). |
2023-11-15 |
2023-11-20 |
Not clear |
| Pei-Qi Huang, Kai-Wen Kang, Dan-Yu Huang, Chun-Lin Zhao, Hao Zheng, Yong-Xin Luo, Yan Wen, Ming-Feng Zou, Dong-Li Li, Ri-Hui Wu, Yun-Cai Tian, Yong Tian, Wen-Huan Zhang, Jing-Wei Jin, Sheng Yin, Li-She Ga. Lignan glucosides from Gentiana macrophylla with potential anti-arthritis and hepatoprotective activities. Phytochemistry. 2023-11-11. PMID:37951561. |
flow cytometry and western blot analysis showed that compound 5 ameliorated d-galn induced aml12 cell apoptosis by upregulating the expression of anti-apoptotic bcl-2 protein and down-regulating the expression of pro-apoptotic bax protein. |
2023-11-11 |
2023-11-20 |
human |
| Qian Wu, Wenrui Yang, Yanju Bi, Yujie Yao, Chengzhi Li, Xiaojing L. Baicalein inhibits apoptosis and autophagy induced by chlorpyrifos exposure to kidney of Cyprinus carpio through activation of PI3K/AKT pathway. Pesticide biochemistry and physiology. vol 196. 2023-11-09. PMID:37945259. |
furthermore, cpf induced the increase of ros level and inhibition of pi3k and nrf2 pathways, which in turn triggered oxidative stress, autophagy and apoptosis in carp kidney according to western blot, rt-qpcr and kit assays. |
2023-11-09 |
2023-11-20 |
Not clear |