| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Min Huang, Junfeng Wang, Zhengrong Zhang, Xueliang Zu. ZMIZ1 Regulates Proliferation, Autophagy and Apoptosis of Colon Cancer Cells by Mediating Ubiquitin-Proteasome Degradation of SIRT1. Biochemical genetics. 2024-01-12. PMID:38214831. |
specific shrna transfection was used to knock down the expression of zmiz1 in colon cancer cell lines (hct116 and ht29), and cell proliferation was detected using edu and cck-8 reagents, apoptosis by flow cytometry, and autophagy by western blot. |
2024-01-12 |
2024-01-14 |
Not clear |
| Xue-Ting Cao, Bo-Ya Wu, Jing Che. [Eleutheroside B induces apoptosis and autophagy of lung cancer cells by regulating PI3K/Akt/mTOR pathway]. Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica. vol 48. issue 24. 2024-01-11. PMID:38212029. |
ao/eb fluorescence double staining and hoechst 33342 fluorescence staining were used to detect the effect of eleutheroside b on apoptosis of lung cancer a549 and h460 cells, and western blot was used to detect apoptosis-related proteins to explore the apoptosis-related molecular mechanism. |
2024-01-11 |
2024-01-14 |
Not clear |
| Mei Cao, Zhisheng Duan, Xianting Wang, Pan Gong, Limei Zhang, Bin Rua. Curcumin Promotes Diabetic Foot Ulcer Wound Healing by Inhibiting miR-152-3p and Activating the FBN1/TGF-β Pathway. Molecular biotechnology. 2024-01-11. PMID:38206528. |
various techniques, including western blot, rt‒qpcr, flow cytometry, transwell, cell scratch test and h&e staining, were employed to measure the levels of relevant genes and proteins, as well as to assess cell proliferation, apoptosis, migration, and pathological changes. |
2024-01-11 |
2024-01-13 |
rat |
| Xiao-Li Min, Wen-Ji Jia, Li Guo, Rui Jing, Xiao-Hong Zhao, Jia-Yi Hu, Xu-Hui Li, Wei Liu, Tao Wang, Xing-Kui Do. Brain microvascular endothelial cell-derived exosomes transmitting circ_0000495 promote microglial M1-polarization and endothelial cell injury under hypoxia condition. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. vol 38. issue 2. 2024-01-09. PMID:38193649. |
endothelial cell damage was assessed by cell viability, apoptosis, apoptosis-related proteins, oxidative stress, and angiogenic activity using cck-8, flow cytometry, western blot, elisa, and endothelial tube formation assay, respectively. |
2024-01-09 |
2024-01-10 |
mouse |
| Wenbo Xu, Shoubin Gu, Gang Zhang, Ren Wang, Songcen Lv, Jinglong Yan, Yong Qi. APOD acts on fibroblast-like synoviocyte and chondrocyte to alleviate the process of osteoarthritis in vitro. Journal of orthopaedic research : official publication of the Orthopaedic Research Society. vol 42. issue 2. 2024-01-09. PMID:37728985. |
subsequently, the inflammatory factors of the two cells were detected by quantitative reverse-transcription polymerase chain reaction, enzyme-linked immunosorbent assay, and western blot, and the apoptosis and autophagy-related substances were determined at the same time. |
2024-01-09 |
2024-01-10 |
Not clear |
| Qionghua Hu, Lingqin Qiu, Li Ge, Yi We. Sevoflurane postconditioning alleviates hypoxic-ischemic brain damage in rats by inhibiting the endoplasmic reticulum stress PERK/ATF4/CHOP pathway. Tissue & cell. vol 86. 2024-01-09. PMID:38194851. |
additionally, nissl staining assessed hippocampal ca1 area neuronal density, tunel staining evaluated hippocampal ca1 area neuronal apoptosis, and western blot determined hippocampal ca1 area protein levels, including bax, bcl-2, p-perk/perk, p-eif2/eif2, atf4, chop, grp78, bax, and bcl-2 protein levels. |
2024-01-09 |
2024-01-13 |
rat |
| Saurabh Shah, Paras Famta, Rahul Kumar, Anamika Sharma, Ganesh Vambhurkar, Giriraj Pandey, Gurpreet Singh, Prakash Kumar, Ankit Mehra, Atul Mourya, Dadi A Srinivasarao, Akshay Shinde, Sajja Bhanu Prasad, Dharmendra Kumar Khatri, Jitender Madan, Saurabh Srivastav. Quality by design fostered fabrication of cabazitaxel loaded pH-responsive Improved nanotherapeutics against prostate cancer. Colloids and surfaces. B, Biointerfaces. vol 234. 2024-01-05. PMID:38181691. |
western blot studies revealed an elevation in caspase-1 and suppression in bcl-2 indicating enhanced apoptosis compared to the control group. |
2024-01-05 |
2024-01-08 |
Not clear |
| Sheng Wang, Bang-Shuo Zhang, Yi Yang, Lin-Lin F. CircFN1 promotes acute myeloid leukemia cell proliferation and invasion but refrains apoptosis via miR-1294/ARHGEF10L axis. The Kaohsiung journal of medical sciences. 2024-01-05. PMID:38180276. |
cck-8 was used to detect cell proliferation, transwell to cell invasion, tunel staining and flow cytometry to detect cell apoptosis, rt-qpcr to circfn1 rna, mir-1294, and arhgef10l expression levels in hl-60 cells, and western blot to arhgef10l protein expression level in hl-60 cells. |
2024-01-05 |
2024-01-07 |
Not clear |
| Chunyan Wu, Qiao Song, Sophie Gao, Shaoling W. Targeting HDACs for diffuse large B-cell lymphoma therapy. Scientific reports. vol 14. issue 1. 2024-01-03. PMID:38168914. |
chidamide was found to have a cytotoxic effect on dlbcl cells in a dose-dependent manner, while transcriptome analysis and western blot revealed that using it for treatment impacted several biological processes, including pi3k/akt signaling, mtor signaling, the cell cycle, and apoptosis pathways. |
2024-01-03 |
2024-01-06 |
Not clear |
| Cairui Li, Shuguang Sun, Yanmei Zhuang, Zhaokui Luo, Guangquan Ji, Zhong Li. CTSB Nuclear Translocation Facilitates DNA Damage and Lysosomal Stress to Promote Retinoblastoma Cell Death. Molecular biotechnology. 2023-12-30. PMID:38159170. |
real-time quantitative polymerase chain reaction (rt-qpcr), western blot analysis, a comet assay, a cell counting kit-8 (cck-8) assay and flow cytometry were used to measure the levels of the interrelated genes and proteins and to evaluate alterations in autophagy, apoptosis, proliferation, dna damage and cell cycle arrest. |
2023-12-30 |
2024-01-05 |
mouse |
| Qi Tang, Yanghai Zhang, Linxiu Yue, Hongying Ren, Chuanying Pa. Ssc-miR-429 expression proliles and functions on inducing Leydig cells apoptosis. Theriogenology. vol 216. 2023-12-29. PMID:38157808. |
in this study, we examined the expression of mir-429 in seven pig tissues (heart, liver, spleen, lung, kidney, testis, epididymis, brain) and investigated its impact on the proliferation and apoptosis of testicular interstitial cells using various techniques such as cck-8, edu, tunel, western blot, among others. |
2023-12-29 |
2024-01-05 |
Not clear |
| Xiaomei Zhang, Jian Wang, Haiying L. RTKN2 knockdown alleviates the malignancy of breast cancer cells by regulating the Wnt/β-catenin pathway. Scientific reports. vol 13. issue 1. 2023-12-28. PMID:38155217. |
fcm and western blot assay were performed to measure the function of rtkn2 silencing on bc cell apoptosis. |
2023-12-28 |
2023-12-31 |
Not clear |
| Dan Cui, Zexi Li, Chao Wei, Qianjin Zhang, Can Xia. Long non-coding RNA LINC00491 accelerates head and neck squamous cell carcinoma progression through regulating miR-508-3p/SATB1 axis and activating Wnt signaling pathway. Cytokine. vol 175. 2023-12-27. PMID:38150791. |
functionally, we carried out a series of assays to measure cell proliferation, apoptosis, migration and invasion, such as edu assay, colony formation, wound healing and western blot assays. |
2023-12-27 |
2023-12-30 |
Not clear |
| Lihong Zhang, Qingzi Guo, Ran An, Shuhan Shen, Lin Yi. In vitro ischemic preconditioning mediates the Ca Brain research. 2023-12-23. PMID:38141801. |
cell viability was detected by mtt, apoptosis by hoechst 33,258 staining, the levels of tnf-α and il-1β mrna by rt-qpcr and elisa, and the levels of can, nfat, cox-2 by rt-qpcr and western blot. |
2023-12-23 |
2023-12-25 |
Not clear |
| Mine Caglayan, Sibel Ozde. Potential impacts of bisphenols on prostate cells: An overview of cytotoxicity, proliferation, oxidative stress, apoptosis, and ER-stress response activation. Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association. 2023-12-22. PMID:38134982. |
pnt1a and pc-3 were treated with bpa, bpf and bps at concentrations of 0.1, 1 and 10 μm for 48 h cytotoxicity, brdu cell proliferation, ros generation, apoptosis detection, gene expression analysis and western blot analysis were performed. |
2023-12-22 |
2023-12-25 |
human |
| Ling-Ray Tai, Yi-Fen Chiang, Ko-Chieh Huang, Hsin-Yuan Chen, Mohamed Ali, Shih-Min Hsi. Hinokitiol as a modulator of TLR4 signaling and apoptotic pathways in atopic dermatitis. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie. vol 170. 2023-12-21. PMID:38128179. |
additionally, the cell culture model enabled the evaluation of apoptosis and ros levels using mtt assay, crystal violet staining, western blot, and dcfda assays. |
2023-12-21 |
2023-12-24 |
Not clear |
| Gao Yuan, Ning Li, Xue Qing, Wu Lujia, Huang Kai, Xu Xiangyang, Yuan Ye, Li Qin, Liu Xiaohong, Han Li. BFGF attenuates aortic valvular interstitial cell calcification by inhibiting endoplasmic reticulum stress-mediated apoptosis. Experimental cell research. 2023-12-19. PMID:38113969. |
the protein expression level was detected by western blot, and apoptosis was determined by flow cytometry. |
2023-12-19 |
2023-12-23 |
rat |
| Ting-Ting Li, Jie Chen, En-Sheng Ji, Ya-Jing Gu. [Effect and mechanism of Danggui Buxue Decoction-containing serum in mitigating H9c2 cell injury caused by exposure to intermittent low oxygen]. Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica. vol 48. issue 21. 2023-12-19. PMID:38114184. |
western blot was employed to determine the expression levels of the autophagy-related proteins microtubule-associated proteins light chain 3Ⅱ(lc3Ⅱ), microtubule-associated proteins light chain 3Ⅰ(lc3Ⅰ), p62, parkin and apoptosis related proteins pro caspase-3, caspase-3, b-cell lymphoma-2(bcl-2), bcl-2-associated x(bax). |
2023-12-19 |
2023-12-23 |
Not clear |
| Xinru Yang, Jian Liang, Yue Shu, Linlin Wei, Cailing Wen, Hui Luo, Liqing Ma, Tian Qin, Bin Wang, Siyu Zeng, Ying Liu, Chun Zho. Asperosaponin VI facilitates the regeneration of skeletal muscle injury by suppressing GSK-3β-mediated cell apoptosis. Journal of cellular biochemistry. 2023-12-11. PMID:38079224. |
apoptosis and autophagy-related protein expression were evaluated t s by western blot, and muscle regeneration markers were quantified by quantitative polymerase chain reaction. |
2023-12-11 |
2023-12-17 |
mouse |
| Ramadan F Abdelaziz, Ahmed M Hussein, Mohamed H Kotob, Christina Weiss, Krzysztof Chelminski, Tamara Stojanovic, Christian R Studenik, Mohammed Auf. Enhancement of Radiation Sensitivity by Cathepsin L Suppression in Colon Carcinoma Cells. International journal of molecular sciences. vol 24. issue 23. 2023-12-09. PMID:38069428. |
in addition, the study conducted protein expression analysis by western blot and immunofluorescence assay, cytotoxicity mtt, and apoptosis assays. |
2023-12-09 |
2023-12-17 |
Not clear |