| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| M Fasshauer, J Klein, K M Kriauciunas, K Ueki, M Benito, C R Kah. Essential role of insulin receptor substrate 1 in differentiation of brown adipocytes. Molecular and cellular biology. vol 21. issue 1. 2001-01-18. PMID:11113206. |
using differentiation protocols, both with and without insulin, preadipocyte cell lines derived from irs-1 ko mice exhibited a marked decrease in differentiation and lipid accumulation (10 to 40%) compared to wild-type cells (90 to 100%). |
2001-01-18 |
2023-08-12 |
mouse |
| M Fasshauer, J Klein, K Ueki, K M Kriauciunas, M Benito, M F White, C R Kah. Essential role of insulin receptor substrate-2 in insulin stimulation of Glut4 translocation and glucose uptake in brown adipocytes. The Journal of biological chemistry. vol 275. issue 33. 2000-09-21. PMID:10829031. |
to study the metabolic effects of these proteins in more detail, we established brown adipocyte cell lines from wild type and various irs knockout (ko) animals and characterized insulin action in these cells in vitro. |
2000-09-21 |
2023-08-12 |
mouse |
| M Fasshauer, J Klein, K Ueki, K M Kriauciunas, M Benito, M F White, C R Kah. Essential role of insulin receptor substrate-2 in insulin stimulation of Glut4 translocation and glucose uptake in brown adipocytes. The Journal of biological chemistry. vol 275. issue 33. 2000-09-21. PMID:10829031. |
insulin signaling studies revealed a total loss of irs-2-associated phosphatidylinositol (pi) 3-kinase activity and a reduction in phosphotyrosine-associated pi 3-kinase by 30% (p < 0.05) in the ko cells. |
2000-09-21 |
2023-08-12 |
mouse |
| R R Shankar, Y Wu, H Q Shen, J S Zhu, A D Baro. Mice with gene disruption of both endothelial and neuronal nitric oxide synthase exhibit insulin resistance. Diabetes. vol 49. issue 5. 2000-08-07. PMID:10905473. |
enos ko mice are insulin resistant at the level of the liver and peripheral tissues, whereas the nnos ko mice are insulin resistant only in the latter. |
2000-08-07 |
2023-08-12 |
mouse |
| L Jacobson, L J Muglia, S C Weninger, K Pacák, J A Majzou. CRH deficiency impairs but does not block pituitary-adrenal responses to diverse stressors. Neuroendocrinology. vol 71. issue 2. 2000-08-04. PMID:10686522. |
insulin injection in fasted crh ko mice elicited increases in corticosterone that were markedly lower than those in wild type but marginally significant relative to corresponding ko controls. |
2000-08-04 |
2023-08-12 |
mouse |
| K Sooy, T Schermerhorn, M Noda, M Surana, W B Rhoten, M Meyer, N Fleischer, G W Sharp, S Christako. Calbindin-D(28k) controls [Ca(2+)](i) and insulin release. Evidence obtained from calbindin-d(28k) knockout mice and beta cell lines. The Journal of biological chemistry. vol 274. issue 48. 1999-12-29. PMID:10567411. |
the role of the calcium-binding protein, calbindin-d(28k) in potassium/depolarization-stimulated increases in the cytosolic free ca(2+) concentration ([ca(2+)](i)) and insulin release was investigated in pancreatic islets from calbindin-d(28k) nullmutant mice (knockouts; ko) or wild type mice and beta cell lines stably transfected and overexpressing calbindin. |
1999-12-29 |
2023-08-12 |
mouse |
| K Sooy, T Schermerhorn, M Noda, M Surana, W B Rhoten, M Meyer, N Fleischer, G W Sharp, S Christako. Calbindin-D(28k) controls [Ca(2+)](i) and insulin release. Evidence obtained from calbindin-d(28k) knockout mice and beta cell lines. The Journal of biological chemistry. vol 274. issue 48. 1999-12-29. PMID:10567411. |
in addition to the increase in [ca(2+)](i) in response to kcl there was also a significant increase in insulin release in islets isolated from ko mice. |
1999-12-29 |
2023-08-12 |
mouse |
| K Sooy, T Schermerhorn, M Noda, M Surana, W B Rhoten, M Meyer, N Fleischer, G W Sharp, S Christako. Calbindin-D(28k) controls [Ca(2+)](i) and insulin release. Evidence obtained from calbindin-d(28k) knockout mice and beta cell lines. The Journal of biological chemistry. vol 274. issue 48. 1999-12-29. PMID:10567411. |
in summary, our findings, using both isolated islets from calbindin-d(28k) ko mice and beta cell lines, establish a role for calbindin in the modulation of depolarization-stimulated insulin release and suggest that calbindin can control the rate of insulin release via regulation of [ca(2+)](i). |
1999-12-29 |
2023-08-12 |
mouse |
| A C Gazdag, C L Dumke, C R Kahn, G D Carte. Calorie restriction increases insulin-stimulated glucose transport in skeletal muscle from IRS-1 knockout mice. Diabetes. vol 48. issue 10. 1999-10-19. PMID:10512356. |
to determine whether insulin receptor substrate (irs)-1 is essential for the insulin-sensitizing effect of cr, we measured in vitro 2-deoxyglucose (2dg) uptake in the presence and absence of insulin by skeletal muscle isolated from wild-type (wt) mice and transgenic mice lacking irs-1 (knockout [ko]) after either ad libitum (al) feeding or 20 days of cr (60% of ad libitum intake). |
1999-10-19 |
2023-08-12 |
mouse |
| L Jacobso. Lower weight loss and food intake in protein-deprived, corticotropin releasing hormone-deficient mice correlate with glucocorticoid insufficiency. Endocrinology. vol 140. issue 8. 1999-08-17. PMID:10433210. |
the remaining catabolic response in crh ko mice did not correlate with morning plasma catecholamines or insulin. |
1999-08-17 |
2023-08-12 |
mouse |
| D Bleich, S Chen, B Zipser, D Sun, C D Funk, J L Nadle. Resistance to type 1 diabetes induction in 12-lipoxygenase knockout mice. The Journal of clinical investigation. vol 103. issue 10. 1999-06-07. PMID:10330425. |
12-lo ko mice were highly resistant to diabetes development compared with control mice and had higher serum insulin levels on day 28. |
1999-06-07 |
2023-08-12 |
mouse |
| P S Brzović, W E Choi, D Borchardt, N C Kaarsholm, M F Dun. Structural asymmetry and half-site reactivity in the T to R allosteric transition of the insulin hexamer. Biochemistry. vol 33. issue 44. 1994-12-06. PMID:7947711. |
the zinc-insulin hexamer, the storage form of insulin in the pancreas, is an allosteric protein capable of undergoing transitions between three distinct conformational states, designated t6, t3r3, and r6, on the basis of their ligand binding properties, allosteric behavior, and pseudo point symmetries [kaarsholm, n. c., ko, h.-c., & dunn, m. f. (1989) biochemistry 28, 4427-4435]. |
1994-12-06 |
2023-08-12 |
human |
| U Pante. Effects of alpha-ketomonocarboxylic acids upon insulin secretion and metabolism of isolated pancreatic islets. Naunyn-Schmiedeberg's archives of pharmacology. vol 291. issue 4. 1976-03-05. PMID:1687. |
insulin release in response to alpha-ketooctanoic (ko) acid started slowly and was accompanied by a decrease of the nad(p)h-fluorescence trace. |
1976-03-05 |
2023-08-11 |
Not clear |