| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| K Yonezawa, K Yokono, K Shii, J Hari, S Yaso, T Sakamoto, Y Kawase, H Akiyama, S Taketomi, S Bab. Biological properties of an initial degradation product of insulin by insulin-degrading enzyme. Endocrinology. vol 124. issue 1. 1989-02-07. PMID:2642422. |
to assess whether the initial degradation product of insulin generated by ide preserves biological properties, we analyzed several insulin-like activities of peak vi. |
1989-02-07 |
2023-08-11 |
human |
| K Yonezawa, K Yokono, K Shii, J Hari, S Yaso, T Sakamoto, Y Kawase, H Akiyama, S Taketomi, S Bab. Biological properties of an initial degradation product of insulin by insulin-degrading enzyme. Endocrinology. vol 124. issue 1. 1989-02-07. PMID:2642422. |
on the other hand, cross-linking analysis suggested that peak vi preserves almost the same affinity for ide as does insulin. |
1989-02-07 |
2023-08-11 |
human |
| K Yonezawa, K Yokono, K Shii, J Hari, S Yaso, T Sakamoto, Y Kawase, H Akiyama, S Taketomi, S Bab. Biological properties of an initial degradation product of insulin by insulin-degrading enzyme. Endocrinology. vol 124. issue 1. 1989-02-07. PMID:2642422. |
these results suggest that pig skeletal muscle ide may cleave peptide bonds within the a-chain early in insulin degradation, generating peak vi; this then serves as the next substrate of ide while exerting reduced insulin-like activity, and peak vi is converted to several relatively low mol wt products. |
1989-02-07 |
2023-08-11 |
human |
| J V Garcia, B W Fenton, M R Rosne. Isolation and characterization of an insulin-degrading enzyme from Drosophila melanogaster. Biochemistry. vol 27. issue 12. 1988-11-18. PMID:3139025. |
the high degree of evolutionary conservation between the drosophila and mammalian ide suggests an important role for this enzyme in the metabolism of insulin and also provides further evidence for the existence of a complete insulin-like system in invertebrate organisms such as drosophila. |
1988-11-18 |
2023-08-11 |
drosophila_melanogaster |
| H Akiyama, K Shii, K Yokono, K Yonezawa, S Sato, K Watanabe, S Bab. Cellular localization of insulin-degrading enzyme in rat liver using monoclonal antibodies specific for this enzyme. Biochemical and biophysical research communications. vol 155. issue 2. 1988-10-25. PMID:3048264. |
although insulin-degrading enzyme (ide) has been implicated in the intracellular degradation of insulin, the cellular localization of this enzyme is still controversial. |
1988-10-25 |
2023-08-11 |
rat |
| S Yas. [Kinetic studies of insulin degrading enzyme in cultured human lymphocytes]. Nihon Naibunpi Gakkai zasshi. vol 64. issue 4. 1988-09-20. PMID:3042472. |
n-ethylmaleimide and bacitracin, inhibitors which inhibit the activity of insulin-degrading enzyme (ide), and anti-ide serum were decreased insulin degradation in bri-7 cells which does not contain lysosomal pathway. |
1988-09-20 |
2023-08-11 |
human |
| S Yas. [Kinetic studies of insulin degrading enzyme in cultured human lymphocytes]. Nihon Naibunpi Gakkai zasshi. vol 64. issue 4. 1988-09-20. PMID:3042472. |
the kinetic changes of insulin receptors and cell surface ide was determined in bri-7 cells after preincubation with or without insulin. |
1988-09-20 |
2023-08-11 |
human |
| S Yas. [Kinetic studies of insulin degrading enzyme in cultured human lymphocytes]. Nihon Naibunpi Gakkai zasshi. vol 64. issue 4. 1988-09-20. PMID:3042472. |
bri-7 cells were preincubated with 10(-6) m insulin for 30 min to 18 h. fifty percent of the receptors were lost at 6 h after the preincubation, and level of the receptors achieved a steady state at 18 h. although the loss of surface ide was slightly slower than that of receptors, the curves were essentially parallel to each other. |
1988-09-20 |
2023-08-11 |
human |
| S Yas. [Kinetic studies of insulin degrading enzyme in cultured human lymphocytes]. Nihon Naibunpi Gakkai zasshi. vol 64. issue 4. 1988-09-20. PMID:3042472. |
cell surface insulin receptor was decreased, whereas cytosolic ide was increased in bri-7 cells incubated with insulin. |
1988-09-20 |
2023-08-11 |
human |
| S Yas. [Kinetic studies of insulin degrading enzyme in cultured human lymphocytes]. Nihon Naibunpi Gakkai zasshi. vol 64. issue 4. 1988-09-20. PMID:3042472. |
thus, it is likely that both cell surface and cytosolic ide, acting either individually or in concert, constitute a physiological mechanism by which the cellular response to insulin is terminated. |
1988-09-20 |
2023-08-11 |
human |
| S Yas. [Kinetic studies of insulin degrading enzyme in cultured human lymphocytes]. Nihon Naibunpi Gakkai zasshi. vol 64. issue 4. 1988-09-20. PMID:3042472. |
these results suggest that ide may play a major role in insulin degradation in the intact cell. |
1988-09-20 |
2023-08-11 |
human |
| S Yas. [Kinetic studies of insulin degrading enzyme in cultured human lymphocytes]. Nihon Naibunpi Gakkai zasshi. vol 64. issue 4. 1988-09-20. PMID:3042472. |
moreover, one possible mechanism of intracellular insulin degradation is that cell surface ide may be internalized with the insulin receptor complex and may degrade insulin during the intracellular process. |
1988-09-20 |
2023-08-11 |
human |
| M P Stoppelli, J V Garcia, S J Decker, M R Rosne. Developmental regulation of an insulin-degrading enzyme from Drosophila melanogaster. Proceedings of the National Academy of Sciences of the United States of America. vol 85. issue 10. 1988-06-22. PMID:3130628. |
the potential importance of this insulin protease is illustrated by the fact that there is an ide in drosophila melanogaster kc cells that shares both physical and kinetic properties with its mammalian counterpart. |
1988-06-22 |
2023-08-11 |
drosophila_melanogaster |
| K Yonezawa, K Yokono, K Shii, J Hari, S Yaso, K Amano, T Sakamoto, Y Kawase, H Akiyama, M Nagat. Insulin-degrading enzyme is capable of degrading receptor-bound insulin. Biochemical and biophysical research communications. vol 150. issue 2. 1988-03-09. PMID:3277630. |
in the investigation of the intracellular sites of insulin degradation, it might be important whether receptor-bound insulin could be a substrate for insulin-degrading enzyme (ide). |
1988-03-09 |
2023-08-11 |
rat |
| K Yonezawa, K Yokono, K Shii, J Hari, S Yaso, K Amano, T Sakamoto, Y Kawase, H Akiyama, M Nagat. Insulin-degrading enzyme is capable of degrading receptor-bound insulin. Biochemical and biophysical research communications. vol 150. issue 2. 1988-03-09. PMID:3277630. |
with increasing amounts of ide, the radioactivity of 135 kd band (insulin receptor alpha-subunit) decreased, whereas that of 110 kd band (ide) appeared then gradually increased, suggesting that ide could bind to receptor-bound insulin. |
1988-03-09 |
2023-08-11 |
rat |
| K Yonezawa, K Yokono, K Shii, J Hari, S Yaso, K Amano, T Sakamoto, Y Kawase, H Akiyama, M Nagat. Insulin-degrading enzyme is capable of degrading receptor-bound insulin. Biochemical and biophysical research communications. vol 150. issue 2. 1988-03-09. PMID:3277630. |
during incubation of insulin-receptor complex with ide at 37 degrees c, about half of the [125i]insulin was dissociated from the complex. |
1988-03-09 |
2023-08-11 |
rat |
| K Yonezawa, K Yokono, K Shii, J Hari, S Yaso, K Amano, T Sakamoto, Y Kawase, H Akiyama, M Nagat. Insulin-degrading enzyme is capable of degrading receptor-bound insulin. Biochemical and biophysical research communications. vol 150. issue 2. 1988-03-09. PMID:3277630. |
cross-linked, non-dissociable receptor-bound [125i]insulin was also degraded by ide. |
1988-03-09 |
2023-08-11 |
rat |
| K Yonezawa, K Yokono, K Shii, J Hari, S Yaso, K Amano, T Sakamoto, Y Kawase, H Akiyama, M Nagat. Insulin-degrading enzyme is capable of degrading receptor-bound insulin. Biochemical and biophysical research communications. vol 150. issue 2. 1988-03-09. PMID:3277630. |
rebinding studies to im-9 cells showed that the receptor binding activity of dissociated [125i]insulin from insulin-receptor complex incubated with ide was significantly (p less than 0.001) decreased as compared with that without the enzyme. |
1988-03-09 |
2023-08-11 |
rat |
| K Yonezawa, K Yokono, K Shii, J Hari, S Yaso, K Amano, T Sakamoto, Y Kawase, H Akiyama, M Nagat. Insulin-degrading enzyme is capable of degrading receptor-bound insulin. Biochemical and biophysical research communications. vol 150. issue 2. 1988-03-09. PMID:3277630. |
these results, therefore, show that ide could recognize and degrade receptor-bound insulin, and suggest that ide may be involved in insulin metabolism during receptor-mediated endocytosis through the degradation of receptor-bound insulin in early neutral vesicles before their internal ph is acidified. |
1988-03-09 |
2023-08-11 |
rat |
| M Kobayashi, M Iwasaki, N Watanabe, O Ishibashi, Y Takata, T Haruta, T Sasaoka, Y Shigeta, K Inouy. Metabolism of a mutant insulin by a receptor-mediated process and an insulin degrading enzyme. Diabetes research and clinical practice. vol 2. issue 5. 1987-01-08. PMID:3536368. |
the media containing the insulin degrading enzyme (ide) of im-9 cells and rat livers degraded porcine and mutant insulin to the same extent. |
1987-01-08 |
2023-08-11 |
rat |